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"Aspergillus flavus UICC 360 telah diketahui dapat menghasilkan senyawa anti-Candida albicans. Penelitian bertujuan untuk mengetahui pengaruh konsentrasi natrium nitrat terhadap kemampuan anti-C. albicans dari Aspergillus flavus UICC 360. Sebanyak (2,8--3,7) x 107 CFU/ml inokulum Aspergillus flavus UICC 360 dengan konsentrasi 1,96% (v/v), diinokulasikan ke dalam medium Czapek?s Dox Broth yang berisi variasi konsentrasi natrium nitrat (0 mM, 23 mM, 29 mM, 35 mM, 41 mM, dan 47 mM). Fermentasi dilakukan selama 7 hari pada suhu ruang (27--30° C). Pengujian kemampuan anti-C. albicans dilakukan dengan menggunakan metode difusi agar cara cakram. Kemampuan anti-C. albicans ditunjukkan oleh terbentuknya zona hambat. Uji perbandingan berganda Least Significancy Difference (LSD) (P < 0,05) memperlihatkan adanya pengaruh nyata pemberian variasi konsentrasi natrium nitrat terhadap ukuran diameter zona hambat.Hasil penelitian menunjukkan NaNO3 29 mM (ekstrak E3 dalam etil asetat) merupakan konsentrasi terbaik untuk aktivitas anti-C. albicans, ditandai dengan diameter zona hambat, yaitu 8,70 ± 0,53 mm (setara dengan nistatin pada konsentrasi 1.581,8 ppm).

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Aspergillus flavus UICC 360 has been known to produce anti-Candida albicans compound. The research aims to determine the effect of sodium nitrate concentration on Aspergillus flavus UICC 360 in producing anti-C. albicans. Inoculum of (2.8--3.7) x 107 CFU/ml of Aspergillus flavus UICC 360 in 1.96% (v/v) concentration was inoculated into Czapek's Dox Broth medium containing various sodium nitrate concentration (0 mM, 23 mM, 29 mM, 35 mM, 41 mM, and 47 mM).

The fermentation was carried out for 7 days at 27--30° C. Investigation of anti-C. albicans test was carried out by disc agar diffusion method. Anti-C. albicans from Aspergillus flavus UICC 360 was shown by the formation of inhibitory zones. Least Significancy Difference test (P < 0.05) showed significant effect of the varying sodium nitrate concentration on inhibitory zone diameter.

The result showed that highest anti-C. albicans was shown by highest inhibition zone diameter at 8.70 ± 0.53 (equivalent to the activity of nystatin at concentration of 1,581.8 ppm) which was achieved at 29 mM NaNO3 (extract of E3 in ethyl acetate)."

"Penelitian bertujuan untuk mengetahui identitas khamir yang hidup pada putik bunga Ceiba pentandra (L.) Gaertn dan saluran pencernaan Apis mellifera L., lebah pengumpul polen yang mengunjungi bunga Ceiba pentandra. Sebanyak 12 isolat khamir yang terdiri dari tiga isolat dari putik bunga Ceiba pentandra dan sembilan isolat dari saluran pencernaan Apis mellifera digunakan pada penelitian. Isolat-isolat khamir diidentifikasi berdasarkan hasil Basic Local Alignment Searching Tools (BLAST) data sequence daerah ITS rDNA, analisis filogenetik dengan metode Neighbor Joining, dan pengamatan alat reproduksi seksual dan aseksual. Primer forward ITS1 dan primer reverse ITS4 digunakan untuk mengamplifikasi daerah ITS rDNA. Hasil elektroforesis gel produk PCR menunjukkan ukuran daerah ITS rDNA isolat khamir tersebut bervariasi antara 400 hingga 800 pb. Hasil menunjukkan bahwa 12 isolat khamir terdiri dari enam species. Lima species khamir termasuk ke dalam phylum Ascomycota, order Saccharomycetales, class Saccharomycetes dan satu species khamir termasuk ke dalam phylum Basidiomycota, order Tremellales,dan class Tremellomycetes. Tiga isolat khamir dari putik bunga C. pentandra diidentifikasi sebagai Bullera coprosmaensis (JZ137), Candida orthopsilosis (JZ053), dan Debaryomyces hansenii (JZ051). Sembilan isolat khamir dari saluran pencernaan A. mellifera diidentifikasi sebagai Candida fermentatii (JZ059 dan JZ060), Candida mesorugosa (JZ057, JZ058, dan JZ063), Candida orthopsilosis (JZ064 dan JZ065), Candida parapsilosis (JZ066), dan Debaryomyces hansenii (JZ061). Dua species khamir yaitu Candida orthopsilosis dan Debaryomces hansenii ditemukan pada putik C. pentandra dan saluran pencernaan A. mellifera.

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The aim of this study was to identify yeasts from the pistils of Ceiba pentandra (L.) Gaertn and digestive tracts of pollen collecting bee, Apis mellifera L. Twelve yeast isolates were identified which were consisted of three isolates from the pistils of C. pentandra and nine isolates from digestive tracts of A. mellifera. Identification was based on homology sequences analysis using Basic Local Alignment Searching Tools (BLAST), phylogenetic analysis by Neighbor Joining method, and observation of sexual and asexual reproduction. The primer set of ITS1 (forward primer) and ITS4 (reverse primer) were used to amplify ITS region rDNA of the isolates. Gel electrophoresis results showed that the size of ITS region of the isolates were varied on the range of 400--800 bp.

The results showed that twelve yeast isolates were identified as six species. Taxonomically, five species belong to phylum Ascomycota, order Saccharomycetales, class Saccharomycetes and one species belong to phylum Basidiomycota order Tremellales, class Tremellomycetes. Three yeast isolates from the pistils of C. pentandra were identified as Bullera coprosmaensis (JZ137), Candida orthopsilosis (JZ053), and Debaryomyces hansenii (JZ051). Nine yeast isolates from digestive tracts of pollen collecting A. mellifera were identified as Candida fermentatii (JZ059 & JZ060), Candida mesorugosa (JZ057, JZ058, dan JZ063), Candida orthopsilosis (JZ064 & JZ065), Candida parapsilosis (JZ066), and Debaryomyces hansenii (JZ061). Debaryomyces hansenii and Candida orthopsilosis were found on the pistils of C. pentandra and digestive tracts of A. mellifera."

"Aspergillus flavus UICC 360 merupakan fungi yang mampu menghasilkan senyawa metabolit sekunder berupa lovastatin. Penelitian bertujuan untuk mengetahui pengaruh variasi konsentrasi urea terhadap kemampuan Aspergillus flavus UICC 360 dalam menghasilkan lovastatin. Proses fermentasi menggunakan konsentrasi inokulum Aspergillus flavus UICC 360 sebesar 1,96% (v/v) dalam medium Czapek?s Dox Broth (CDB) modifikasi dengan variasi konsentrasi urea (0 mM, 33 mM, 42 mM, 50 mM, 58 mM, dan 67 mM) dan inkubasi selama 7 hari pada suhu ruang (27--300C) dengan kecepatan agitasi 90 rpm. Ekstrak hasil fermentasi dalam etil asetat diuji terhadap Candida albicans UICC Y-29 menggunakan metode difusi agar cara cakram. Ekstrak hasil fermentasi dari konsentrasi urea 42 mM mempunyai indeks penghambatan rata-rata tertinggi sebesar 0,54 ± 0,15. Hasil Kromatografi Lapis Tipis (KLT) menunjukkan bahwa nilai Rf ekstrak hasil fermentasi dari konsentrasi urea 42 mM sama dengan lovastatin standar, yaitu 0,42 yang mengindikasikan ekstrak mengandung lovastatin. Uji Least Significant Difference (LSD) (P < 0,05) menunjukkan terdapat perbedaan nyata variasi konsentrasi urea terhadap kemampuan Aspergillus flavus UICC 360 dalam menghasilkan lovastatin. Hal tersebut menunjukkan bahwa pemberian variasi konsentrasi urea berpengaruh terhadap kemampuan Aspergillus flavus UICC 360 dalam menghasilkan lovastatin.

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Aspergillus flavus UICC 360 is capable of producing secondary metabolites such as lovastatin. The study aims to determine the effect of variations of urea concentration on the ability of Aspergillus flavus UICC 360 to produce lovastatin. The fermentation process using 1.96% (v/v) inoculum concentration of Aspergillus flavus UICC 360 in the Czapek?s Dox Broth (CDB) medium modified with urea concentration variations (0 mM, 33 mM, 42 mM, 50 mM, 58 mM, and 67 mM) and incubated for 7 days at room temperature (27--30 °C) with agitation speed of 90 rpm. Ethyl acetate extracts were tested against Candida albicans UICC Y-29 using agar disc diffusion method. The extract from fermentation medium of 42 mM urea has the highest average of inhibition index of 0.54 ± 0.15.

Results of Thin Layer Chromatography (TLC) showed that the extract from fermentation medium of 42 mM urea has the same Rf value with lovastatin standard Rf 0.42 which indicated that the extract contained lovastatin. Least Significant Difference (LSD) test showed that there were significant difference in the urea concentration variation in the ability of Aspergillus flavus UICC 360 to produce lovastatin. It shows that variation of urea concentrations affect the ability of Aspergillus flavus UICC 360 to produce lovastatin."

"ABSTRAKAspergillus flavus UICC 360 telah diketahui mampu menghasilkan lovastatinpada fermentasi menggunakan sumber nitrogen NaNO3. Penelitian bertujuanuntuk mengetahui pengaruh variasi konsentrasi NH4NO3 terhadap kemampuankapang tersebut dalam menghasilkan lovastatin. Fermentasi menggunakanmedium Czapek’s Dox Broth modifikasi dengan variasi konsentrasi NH4NO3 (0mM; 25,00 mM; 31,25 mM; 37,50 mM; 43,75 mM; dan 50,00 mM). Aspergillusflavus UICC 360 dengan konsentrasi inokulum sebesar 1,96% (v/v)diinokulasikan ke dalam medium, kemudian diagitasi 90 rpm, pada suhu ruang(27o--30oC) selama 7 hari untuk mendapatkan ekstrak hasil fermentasi. Pengujianekstrak lovastatin di dalam etil asetat dilakukan terhadap Candida albicans UICCY-29 dengan metode difusi agar cara cakram. Ekstrak hasil fermentasi denganperlakuan 37,50 mM NH4NO3 menunjukkan indeks penghambatan tertinggi, yaitusebesar 0,84 ± 0,07. Hasil Kromatografi Lapis Tipis (KLT) ekstrak hasilfermentasi perlakuan 25,00 mM NH4NO3 dan 37,50 mM NH4NO3 memiliki Rf(0,45), perlakuan 31,25 mM NH4NO3 dan 43,75 mM NH4NO3 memiliki Rf (0,47),sedangkan nilai Rf perlakuan 50 mM NH4NO3 (0,48). Nilai Rf ekstrak hasilfermentasi tersebut hampir sama dengan Rf lovastatin standar, yaitu (0,46),sehingga mengindikasikan adanya senyawa lovastatin di dalam ekstrak. Hasil ujiperbandingan berganda Least Significant Differences (LSD) (P < 0,05)menunjukkan adanya pengaruh nyata pemberian variasi konsentrasi NH4NO3terhadap kemampuan A. flavus UICC 360 dalam menghasilkan lovastatin.

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ABSTRACTAspergillus flavus UICC 360 has been reported to produce lovastatin infermentation by using nitrogen source such as NaNO3. The research aims todetermine the effect of variations of NH4NO3 concentration on the ability of A.flavus UICC 360 to produce lovastatin. Fermentation was carried out by usingCzapek's Dox Broth modified with variations of NH4NO3 concentration (0 mM;25.00 mM; 31.25 mM; 37.50 mM; 43.75 mM; and 50.00 mM). Aspergillus flavusUICC 360 with inoculum concentration of 1.96% (v/v) was inoculated into themedium and then agitated 90 rpm, at room temperature (27o--30oC) for 7 days toobtain the fermentation extract. Extract in ethyl acetate was tested with a discdiffusion method against Candida albicans UICC Y-29. The extract from thefermentation using 37.50 mM NH4NO3 showed the highest inhibition index 0.84± 0.07. The results of Thin Layer Chromatography (TLC) of extract from thefermentation of using 25.00 mM NH4NO3 and 37.50 mM NH4NO3 have Rf (0.45),31.25 mM NH4NO3 and 43.75 mM NH4NO3 have Rf (0.47), and 50 mM NH4NO3have Rf (0.48). The Rf value of extracts have nearly similiar with a lovastatinstandard 0.46 which indicated there was lovastatin in the extract. The results ofLeast Significant Differences (LSD) (P <0.05) showed there was a significanteffect of NH4NO3 concentration variation in the ability of A. flavus UICC 360 toproduce lovastatin."

"ABSTRAKAspergillus flavus UICC 360 koleksi Universitas Indonesia Culture Collection (UICC) telah diteliti dan diuji mampu menghasilkan lovastatin. Penelitian bertujuan untuk mengetahui pengaruh variasi konsentrasi amonium sulfat (0 mM, 15,15 mM, 18,94 mM, 22,73 mM, 26,52 mM, dan 30,30 mM) sebagai sumber nitrogen terhadap kemampuan Aspergillus flavus UICC 360 menghasilkan lovastatin. Fermentasi menggunakan 1,96% (v/v) inokulum sel kapang selama 7 hari pada medium Czapek?s Dox Broth modifikasi dalam suhu ruang (27--30oC) dengan pengocokan 90 rpm. Ekstrak dalam etil asetat diuji terhadap Candida albicans UICC Y-29 dengan metode difusi agar cara cakram. Ekstrak hasil fermentasi dari perlakuan 22,73 mM amonium sulfat memiliki kemampuan tertinggi menghambat Candida albicans UICC Y-29 dengan indeks penghambatan rata-rata 0,94 ± 0,06. Hasil Kromatografi Lapis Tipis (KLT) menunjukkan bahwa ekstrak hasil fermentasi perlakuan amonium sulfat 15,15 mM memiliki nilai Rf sama dengan lovastatin standar sebesar 0,48. Ekstrak hasil fermentasi perlakuan amonium sulfat 18,94 mM, 22,73 mM, 26,52 mM, dan 30,30 mM memiliki nilai Rf hampir sama dengan nilai Rf lovastatin standar. Hasil KLT tersebut dapat mengindikasikan ekstrak mengandung lovastatin. Uji Least Significant Difference (LSD) (P<0,05) menunjukkan ada perbedaan nyata variasi konsentrasi amonium sulfat terhadap kemampuan Aspergillus flavus UICC 360 menghasilkan lovastatin. Hal tersebut menunjukkan bahwa terdapat pengaruh variasi konsentrasi amonium sulfat terhadap kemampuan Aspergillus flavus UICC 360 dalam menghasilkan lovastatin.

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ABSTRACTThe ability of Aspergillus flavus UICC 360 to produce lovastatin had been shown in previous study. The aim of this study is to determine the effect of variation in ammonium sulphate concentration at 0 mM, 15.15 mM, 18.94 mM, 22.73 mM, 26.52 mM, and 30.30 mM toward the ability of Aspergillus flavus UICC 360 in producing lovastatin. Fermentation was carried out by using 1.96% (v/v) of inoculum in modified Czapek?s Dox Broth for seven days at room temperature (27--30oC) with 90 rpm agitation. The extract in ethyl acetate was tested by disk diffusion method against Candida albicans UICC Y-29. The extract from fermentation of 22.73 mM ammonium sulphate showed the highest inhibition index of 0.94 ± 0.06. The result of Thin Layer Chromatography (TLC) showed that extract from fermentation of 15.15 mM ammonium sulphate had similar Rf value with lovastatin standard. Meanwhile, extract from fermentation of 18.94 mM, 22.73 mM, 26.52 mM, and 30.30 mM ammonium sulphate had nearly similar Rf value with lovastatin standard. The TLC result indicated that the extract contained lovastatin. Least Significant Difference test (LSD) (P<0.05) showed there was significant difference of variation in ammonium sulphate concentration toward the ability of Aspergillus flavus UICC 360 to produce lovastatin. The result of this study showed that the variation in ammonium sulphate concentration affect the ability of Aspergillus flavus UICC 360 in producing lovastatin."

"Penelitian ini bertujuan untuk memperoleh isolat Actinobacteria termofilik potensial dari tanah di sekitar geiser Cisolok yang dapat mendegradasi xylan dan mengetahui hubungan kekerabatannya dengan taksa terdekat dari Actinobacteria penghasil xylanase. Tujuh belas isolat Actinobacteria termofilik diisolasi dari tanah di sekitar geiser Cisolok, Jawa Barat. Penapisan kemampuan 17 isolat Actinobacteria dan type strain Actinomadura keratinilytica NBRC 105837T mendegradasi xylan dilakukan menggunakan medium Minimal (Mm) padat dengan penambahan substrat xylan 0,5, inkubasi selama 7 hari. Pewarnaan dengan Congo red 0,2 (b/v) menunjukkan terbentuknya zona bening di sekitar koloni isolat Actinobacteria yang dapat mendegradasi xylan 0,5 pada suhu 45 C (15 isolat), 50 C (14 isolat), 55 C (4 isolat), dan 60 C (3 isolat). Type strain NBRC 105837T dapat mendegradasi xylan 0,5 pada suhu 45 C hingga 60 C. Tiga isolat (SL1- 2-R-2, SL1-2-R-3, dan SL1-2-R-4) yang mendegradasi xylan 0,5 hingga suhu 60 C dipilih sebagai isolat potensial. Tiga isolat potensial dan type strain NBRC 105837 dapat mendegradasi substrat Remazol Brilliant Blue R-xylan (RBB-xylan) 0,1 pada medium Mm padat setelah 3 hari inkubasi pada suhu 45 hingga 60 C. Tiga isolat potensial telah diidentifikasi pada penelitian sebelumnya sebagai Actinomadura keratinilytica berdasarkan karakter genotip dan fenotip. Crude enzyme dari tiga isolat potensial dan type strain NBRC 105837 dapat mendegradasi xylan 0,5 dan RBB-xylan 0,1 pada medium Mm padat setelah 24 jam inkubasi pada suhu 45 hingga 60 C. Berdasarkan analisis filogenetik sequence gen 16S rRNA menggunakan metode neighbor-joining, minimum evolution, dan maximum likelihood, 3 isolat potensial membentuk clade yang monofiletik dengan dua spesies Actinomadura termofilik yang dapat mendegradasi xylan (A. keratinilytica dan A. miaoliensis). Tiga isolat potensial membentuk clade yang monofiletik dengan empat spesies Actinomadura termofilik (A. keratinilytica, A. miaoliensis, A. rubrobrunea, dan A. viridilutea). Tiga isolat potensial menghasilkan miselium substrat yang bercabang dan tidak berfragmen, serta miselium aerial yang menghasilkan spora pada medium modified Bennetts padat setelah 14 hari inkubasi pada suhu 45 C. Penelitian ini memberikan informasi tambahan mengenai kemampuan typestrain A. keratinilytica NBRC 105837 mendegradasi xylan.

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The aims of this study were to obtain the potential xylan-degrading thermophilic Actinobacteria isolates from soil of Cisolok geysers and to understand their relationship with the closely related taxa of xylanase-producing Actinobacteria. Seventeen thermophilic Actinobacteria isolates were isolated from soil collected around Cisolok geysers, West Java. Xylan-degrading ability of 17 Actinobacteria isolates and type strain Actinomadura keratinilytica NBRC 105837T were screened by using Minimal (Mm) agar medium with the addition of 0.5 xylan substrate, incubated for 7 days. Clear zone was formed around the colony of Actinobacteria isolates which showed xylan-degrading ability at 45 C (15 isolates), 50 C (14 isolates), 55 C (4 isolates), and 60 C (3 isolates) after staining by 0.2 (w/v) Congo red. Type strain NBRC 105837T was able to degrade 0,5 xylan at 45 to 60 C. Three isolates (SL1-2-R-2, SL1-2-R-3, dan SL1-2-R-4) that showed xylan-degrading ability at 45 to 60 C were choosen as potential isolates. Three potential isolates and type strain NBRC 105837T were able to degrade 0,1 Remazol Brilliant Blue R-xylan (RBB-xylan) substrate on Mm agar after 3 days incubation at 45 to 60 C. In the previous study, these potential isolates were identified as Actinomadura keratinilytica based on genotypic and phenotypic characters. Crude enzyme of 3 potential isolates and type strain NBRC 105837T were able to degrade both 0.5 xylan and 0.1 RBB-xylan on Mm agar after 24 hours at 45 to 60 C. Phylogenetic analyses based on 16S rRNA gene using neighbor-joining, minimum evolution, and maximum likelihood methods showed the 3 potential isolates formed monophyletic clade with two thermophilic xylan-degrading Actinobacteria species (A. keratinilytica and A. miaoliensis). Three potential isolates formed monophyletic clade with four thermophilic Actinobacteria species (A. keratinilytica, A. miaoliensis, A. rubrobrunea, and A. viridilutea). These isolates produced non-fragmented branched substrate mycelia and spores produced from aerial mycelia after 14 days incubation at 45 C. This study reports a new information regarding the xylan-degrading ability of A. keratinilytica NBRC 105837."

"Tujuan dari penelitian ini adalah untuk memperoleh isolat 'Actinobacteria' termofilik dari tanah di sekitar geiser Cisolok, Jawa Barat yang memiliki aktivitas selulolitik pada suhu tinggi serta mengetahui posisi filogenetik isolat terpilih terhadap spesies-spesies terdekatnya berdasarkan gen 16S rRNA. Penapisan kemampuan degradasi selulosa 17 isolat dilakukan secara kualitatif pada 'Minimal medium' (Mm) padat yang ditambahkan substrat yaitu 'carboxymethyl cellulose' (CMC) 1% (b/v) atau  'microcrystalline cellulose' (MCC) 1% (b/v) kemudian diinkubasi selama 7 hari. Pengamatan dilakukan dengan pewarnaan 'Congo red' 0,2% (b/v) dan zona bening pada sekitar koloni mengindikasikan degradasi substrat. Hasil penapisan menunjukkan bahwa 15 isolat mendegradasi CMC 1% dan 12 isolat mendegradasi MCC 1% pada suhu 45 ^oC, 14 isolat mendegradasi CMC 1% dan MCC 1% pada suhu 50 ^oC, 4 isolat mendegradasi CMC 1% dan MCC 1% pada suhu 55 ^oC, dan 3 isolat mendegradasi CMC 1% dan MCC 1% pada suhu 60 ^oC. Tiga isolat (SL1-2-R-2, SL1-2-R-3, dan SL1-2-R-4) yang mendegradasi CMC 1% dan MCC 1% hingga 60 ^oC merupakan isolat terpilih. Identifikasi dan karakterisasi telah dilakukan pada penelitian sebelumnya dan melaporkan tiga isolat terpilih memiliki kekerabatan terdekat dengan 'Actinomadura keratinilytica' WCC-2665^T(=NBRC 105837^T). Hasil pengujian menunjukkan 'type strain' NBRC 105837^T mendegradasi CMC 1% dan MCC 1% pada medium Mm padat dengan suhu 45, 50, 55, dan 60 ^oC setelah inkubasi 7 hari. 'Crude enzyme' dari tiga isolat potensial dan 'type strain' NBRC 105837^T menunjukkan aktivitas selulolitik pada medium Mm padat yang ditambahkan CMC 1% atau MCC 1% pada suhu 45, 50, 55, dan 60 ^oC. Analisis filogenetik tiga isolat terpilih berdasarkan gen 16S rRNA menggunakan metode 'Neighbor-Joining' (NJ), 'Minimum Evolution' (ME), dan 'Maximum Likelihood' (ML) menunjukkan bahwa tiga isolat terpilih berada pada satu 'clade' monofiletik dengan 'Actinomadura' 'keratinilytica' WCC-2665^T. Analisis filogenetik juga menunjukkan dua kelompok yang terpisah berdasarkan kemampuan menghasilkan selulase pada anggota famili 'Thermomonosporaceae'.

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The aims of this study were to obtained thermophilic 'Actinobacteria' isolates from soil around Cisolok geyser, West Java with the ability to degrade cellulose at high temperatures and to analyze the phylogenetic position based on 16S rRNA gene of the selected isolates compared to closely related species. Cellulose degradation screening was performed on Minimal (Mm) medium with the addition of 1% (w/v) carboxymethyl cellulose (CMC) or 1% (w/v) microcrystalline cellulose (MCC) as substrate then incubated for 7 days. Cellulose degradations were observed by staining the plates with  0,2% (w/v) Congo red and clear zone formation around the bacterial colony would indicate the cellulose degradation. The results showed that 15 isolates were able to degrade 1% CMC and 12 isolates were able to degrade 1% MCC at 45 ^oC, 14 isolates were able to degrade 1% CMC and 1% MCC at 50 ^oC, 4 isolates were able to degrade 1% CMC and 1% MCC at 55 ^oC, and 3 isolates were able to degrade 1% CMC and 1% MCC at 60 ^oC. Three isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) were selected due to their CMC and MCC degrading ability at 60 ^oC. Molecular identification based on 16S rRNA gene and characterization in previous study showed that the three selected isolates are closely related to 'Actinomadura keratinilytica' WCC-2665^T(=NBRC 105837^T). The assay showed that type strain NBRC 105837^T was able to degrade 1% CMC and 1% MCC at 45, 50, 55, and 60 ^oC after 7 days of incubation. Cellulolytic activity show that the crude enzymes of the three selected isolates and type strain were able to degrade 1% CMC and 1% MCC at 45, 50, 55, and 60 ^oC. Phylogenetic analysis using Neighbour-Joining (NJ), Minimum Evolution (ME), and Maximum Likelihood (ML) methods showed that the  three selected isolates  were  clustered  together in monophyletic clade with 'Actinomadura keratinilytica' WCC-2265^T with 100% bootstrap value. Phylogenetic analysis also showed that cellulase  producers  and  non-cellulase  producers  in 'Thermomonosporaceae' were grouped into different clades."