"Minat masyarakat terhadap produk makanan dan minuman berbasis keju semakin meningkat. Keju mengandung berbagai bahan tambahan pangan, seperti pengawet yang jika dikonsumsi secara berlebih dapat menyebabkan penyakit. Penelitian ini dilakukan untuk menghasilkan metode analisis nisin dan kalium sorbat secara simultan yang tervalidasi serta menetapkan kadarnya pada sampel dan batas konsumsi hariannya. Dilakukan pengembangan metode analisis menggunakan kromatografi cair kinerja tinggi (KCKT) dengan detektor UV-Vis dan didapatkan kondisi optimum analisis menggunakan kolom Waters® Spherisorb ODS2 C18 (250 × 4,6 mm, 5 μm), fase gerak dapar amonium asetat (pH 4,2):asetonitril (40:60 v/v) dengan elusi isokratik pada panjang gelombang 233 nm, laju alir 1,0 mL/menit, dan volume injeksi 10 μL. Waktu retensi kedua senyawa berada pada rentang 2,401-3,937 menit. Metode yang dikembangkan telah tervalidasi sesuai dengan pedoman Farmakope Indonesia Edisi VI dan ICH dengan rentang nilai r, LOD, LOQ, persentase perolehan kembali, dan koefisien variasi dari uji presisi secara berturut-turut 0,9997-0,9998; 0,000284-0,0368 mg/mL; 0,000863-0,112 mg/mL; 99,73-101,85%; dan 1,021-1,215%. Didapatkan rentang kadar nisin 9,703-16,128 mg/kg produk keju dan rentang kadar kalium sorbat 807,049-1371,641 mg/kg produk keju. Kemudian didapatkan batas konsumsi harian sampel pada kelompok umur 19-29 tahun, yaitu 0,979-1,663 kg produk keju.
Public interest in cheese-based food and beverage products is increasing. Cheese contains various food additives, such as preservatives, which if consumed excessively can cause diseases. The purpose of this study was to produce a validated method for simultaneous analysis of nisin and potassium sorbate and to determine their content in samples and their daily consumption limits. The development of an analytical method using HPLC with a UV-Vis detector was carried out and the optimum analysis were obtained using a Waters® Spherisorb ODS2 C18 column (250 × 4.6 mm, 5 μm), a mobile phase of ammonium acetate buffer (pH 4.2): acetonitrile (40:60 v/v) with isocratic elution at a wavelength of 233 nm, a flow rate of 1.0 mL/min, and an injection volume of 10 μL. The retention time of both compounds was 2.401-3.937 minutes. The developed method has been validated in accordance with the guidelines of the Indonesian Pharmacopoeia Edition VI and ICH with a range of r values, LOD, LOQ, percentage recovery, and coefficient of variation from the precision test respectively 0.9997-0.9998; 0.000284-0.0368 mg/mL; 0.000863-0.112 mg/mL; 99.73-101.85%; and 1.021-1.215%. The range of nisin levels was 9.703-16.128 mg/kg of cheese products and the range of potassium sorbate levels was 807.049-1371.641 mg/kg of cheese products. Then the daily consumption limit of samples in the 19-29 year age group was 0.979-1.663 kg of cheese products."