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"Advances in stem cell research discusses recent advances in stem cell science, including therapeutic applications. This volume covers such topics as biomanufacturing iPS cells for therapeutic applications, techniques for controlling stem cell fate decisions, as well as current basic research in such areas as germ line stem cells, genomics and proteomics in stem cell research. "

"Skripsi ini membahas mengenai pertanggungjawaban hukum bagi dokter dan klinik kecantikan yang menyediakan layanan stem cell untuk perawatan estetika beserta analisis penulis terhadap Putusan Pengadilan Negeri Jakarta Selatan Nomor 563/Pid.Sus/2020/PN.Jkt.Sel. Fokus dari penelitian ini adalah mengenai legalitas penggunaan stem cell di Indonesia baik untuk tujuan pelayanan kesehatan maupun untuk digunakan dalam perawatan estetika. Bentuk penelitian skripsi ini adalah yuridis normatif dengan tipe deskriptif dan metode kualitatif. Hasil penelitian ini menyimpulkan bahwa penggunaan stem cell di Indonesia untuk tujuan penyembuhan penyakit maupun perawatan estetika diperbolehkan secara terbatas pada fasilitas pelayanan kesehatan yang telah mendapatkan izin dari kementerian kesehatan. Putusan Nomor 563/Pid.Sus/2020/PN.Jkt.Sel merupakan putusan yang menjatuhkan hukuman pidana terhadap seorang dokter yang menggunakan stem cell untuk perawatan estetika pada pasiennya. Hasil penelitian ini menyarankan agar pemerintah terutama kementerian beserta organisasi kedokteran yang berwenang untuk segera mengeluarkan pengaturan terkait kompetensi dokter yang diperbolehkan menggunakan stem cell baik untuk pelayanan kesehatan maupun untuk perawatan estetika sehingga kepastian hukum bagi dokter yang melakukan praktik stem cell dapat terwujud.

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This study discusses legal accountability for doctors and beauty clinics that provide stem cell for aesthetic treatment along with the author's analysis of the South Jakarta District Court Verdict Number 563 / Pid.Sus / 2020 / PN.Jkt.Sel. The focus of this research is on the legality of using stem cells in Indonesia both for health care purposes and for use in aesthetic treatments. The form of this thesis research is normative juridical with descriptive type and qualitative methods. The results of this study conclude that the use of stem cells in Indonesia for the purpose of curing diseases and aesthetic treatments is limited to health care facilities that have obtained permission from the ministry of health. Verdict Number 563 / Pid.Sus / 2020 / PN.Jkt.Sel is a verdict that imposes a criminal sentence on a doctor who uses stem cell for aesthetic treatment of his patient. The results of this study suggest that the government, especially the ministry of health and medical organizations that are authorized to immediately issue regulations regarding the competence of doctors who are allowed to use stem cells for both health services and for aesthetic treatment, so that legal certainty for doctors who uses stem cell in their practicecan be realized."

"Latar Belakang: pulpa memiliki sifat low-compliance yang memengaruhi proses regenerasinya. Tujuan: menganalisis potensi Platelet- Rich Plasma (PRP) Eksosom terhadap regenerasi pulpa gigi secara in-vitro viabilitas sel, aktivitas migrasi, dan ekspresi Vascular Endothelial Growth Factor-A (VEGF-A) hDPSCs. Metodologi: hDPSC sembilan gigi molar tiga dikultur dengan metode enzyme digestion (ED) yang dipanen pada P3 dan P4. Kemudian dikultur di dalam enam media, yaitu: Dulbecco's Modified Eagle Medium (DMEM) dan 10% PRP sebagai kelompok kontrol, dan 0,5%, 1%, dan 5% eksosom dari PRP. Semua kelompok memiliki tiga rangkap biologis (Triplo). Uji viabilitas sel dievaluasi dengan MTT assay, aktivitas migrasi sel dengan Scratch Assay dan Transwell Migration Assay, dan ekspresi VEGF-A dengan Enzyme-Linked Lmmunosorbent Assay (ELISA). Analisis data dilakukan dengan uji One Way ANOVA (p <0,05) serta uji Kruskal-Wallis dan post hoc Mann-Whitney (p <0,05). Hasil: viabilitas hDPSCs tertinggi pada 24, 48 dan 72 jam observasi pada kelompok Eksosom dari PRP 5% (p <0,05). Eksosom dari PRP 5% menunjukkan aktivitas migrasi yang lebih tinggi dibandingkan dengan kelompok lain, meskipun terdapat perbedaan tidak bermakna dengan kontrol PRP 10% (p >0,05). Ekspresi VEGF-A hDPSCs tertinggi terdapat pada kelompok PRP Eksosom 5% pada 72 jam observasi. Kesimpulan: eksosom dari PRP 5% berpotensi menginduksi regenerasi pupa gigi manusia.

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Background: pulp has low-compliance properties that affect its regeneration process. Objective: to analyze the potential of Platelet-Rich Plasma (PRP) exosomes on the regeneration of dental pulp by in-vitro evaluation of cell viability, migration activity, and expression of Vascular Endothelial Growth Factor-A (VEGF-A) hDPSCs. Methodology: hDPSCs of nine third molars cultured by enzyme digestion (ED) method were harvested at P3 and P4. Then cultured in six media, Dulbecco's Modified Eagle Medium (DMEM) and 10% PRP as a control group, and 0.5%, 1%, and 5% exosomes of PRP. All groups had a biological triple (Triplo). Cell viability assay was evaluated by MTT assay, cell migration activity by Scratch Assay and Transwell Migration Assay, and VEGF-A expression by Enzyme-Linked Lmmunosorbent Assay (ELISA). Data analysis was performed using One Way ANOVA (p < 0.05) and Kruskal-Wallis and post hoc Mann-Whitney tests (p < 0.05). Results: The viability of hDPSCs was highest at 24, 48 and 72 hours of observation in the Exosomes group of 5% PRP (p < 0.05). Exosomes from 5% PRP showed higher migratory activity compared to other groups, although there was no significant difference with 10% PRP control (p > 0.05). The highest expression of VEGF-A hDPSCs was found in the 5% PRP Exosomes group at 72 hours of observation. Conclusion: exosomes of 5% PRP have the potential to induce the regeneration of human dental pulp."

"Chemical and functional genomic approaches to stem cell biology and regenerative medicine consolidates the latest information on functional genomics and chemical biology approaches that are used to study and control the fate of stem cells. It discusses new technologies and their recent applications in various areas of stem cell biology, covering :- The use of both embryonic and adult stem cells- A vast array of technologies, including: genome-wide expression analysis, functional genomic profiling with arrayed cDNA and RNAi expression libraries, informatics, chemical genomics, mass spectrometry, and proteomics- The applications of advanced technologies in various areas of stem cell biology, encompassing: self-renewal, differentiation, and reprogramming of different types of embryonic and adult stem cells, as well as regeneration in model organisms- Technological tools for studying the biology of stem cells"