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"Latar Belakang: Sel kanker membutuhkan asam amino untuk metabolismenya telah ditemukan pada beberapa studi. Metabolisme ini meyebabkan profil asam amino mengalami perubahan pada kanker payudara. Profil asam amino pada kanker payudara ini dapat menentukan prognosis penyakit pada kanker payudara.Tujuan: Mengetahui hubungan profil asam amino dengan faktor risiko kanker payudara di RS Ciptomangunkusumo, Indonesia.Metode: Penelitian ini merupakan penelitian cross-sectional pada 19 subjek kanker payudara dan 19 subjek kontrol yang memenuhi kriteria penelitian di RSCM, Jakarta, Indonesia. Asam amino dianalisis menggunakan teknik liquid chromatography. Data tersebut dianalisis menggunakan uji independent sample t-test dan uji Mann-Whitney U.Hasil:Perbedaan profil asam amino pada kanker payudara yang signifikan dibandingkan dengan kontrol ditemukan pada peningkatan asam amino sistin dan penurunan asam amino valin, lisin, histidin, alanin, ornitin, tirosin, glutamin, fenilalanin, dan asam amino prolin. Terdapat signifikansi antara asam amino dengan faktor risiko usia, jumlah paritas, riwayat pemberian ASI, usia menarke, dan kebiasaan berolahraga.

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Background: Cancer cell needs amino acids for its metabolism has been found in recent studies. Amino acids profile changes due to cancer cells’ metabolism. This amino acids profile in breast cancer could determine the prognosis of disease in breast cancer.

Objective: To aimed relationship between amino acids profile in breast cancer with its risk factor in Ciptomangunkusumo Hospital, Indonesia.

Method: A cross-sectional study in 19 breast cancer subjects and 19 control subjects that included into the criteria was conducted in this research. Amino acids was examined with liquid chromatography technique. The data was analyzed with independent sample t-test and Mann-Whitney U test.

Result: There are significant differences of amino acids profile between breast cancer subjects and control subjects, essentially for increased cystine profile and decreased valine, lysine, histidine, alanine, ornithine, tyrosine, glutamine, phenylalanine, and proline. There are also significance between amino acids profile in breast cancer with risk factors, including age, parity, breastfeeding history, menarche age, and workout habit."

"Aplikasi metabolomik dalam analisis subtipe kanker payudara dinilai cukup menjanjikan, salah satunya melalui penilaian profil asam amino. Informasi profil asam amino pada pasien kanker payudara berperan penting dalam tatalaksana pengobatan dan prognosis kanker payudara. Beberapa penelitian sebelumnya menunjukkan suatu pola perubahan asam amino pada pasien kanker payudara dibandingkan kontrol orang sehat, yang mengindikasikan kaitan asam amino dengan kanker payudara. Beberapa asam amino dapat menjadi biomarker yang menunjukkan adanya asosiasi dengan progresivitas kanker maupun subtipe IHK kanker payudara. Penelitian ini bertujuan untuk mengetahui hubungan profil asam amino berdasarkan subtipe imunohistokimia kanker payudara. Penelitian menggunakan studi desain potong lintang yang melibatkan 51 pasien kanker payudara di RSUPN Cipto Mangunkusumo. Sampel darah pasien yang terdiagnosis kanker payudara diukur kadar asam aminonya menggunakan metode HPLC dan nilai yang diperoleh dibandingkan dengan nilai rujukan normal untuk mengetahui adanya pola kenaikan dan penurunan. Pola asam amino akan dianalisis dan diuji asosiasinya berdasarkan pengelompokan subtipe imunohistokimia, yang dibagi menjadi Luminal A, Luminal B+HER2 dan Triple Negative Breast Cancer (TNBC) Sebanyak 51 pasien kanker payudara didominasi oleh kelompok Luminal B+HER2 diikuti oleh Luminal A dan TNBC. Sebagian besar pola asam amino berdasarkan subtype IHK menunjukkan kadar normal, kecuali asam amino arginin dan histidin yang mengalami peningkatan pada kelompok Luminal A dan Luminal B+HER, serta penurunan kadar asam amino ornitin pada kelompok TNBC. Analisis bivariat meunjukkan adanya hubungan yang signifikan (p<0.05) antara asam amino arginin dan ornitin dengan subtipe IHK. Luminal B+HER2 menjadi kelompok subtipe imunohistokimia kanker payudara yang mendominasi. Berdasarkan 19 asam amino yang diuji, asam amino dari ketiga kelompok subtipe imunohistokimia cenderung normal, dimana hanya tiga asam amino yang menunjukkan pola perubahan, yaitu histidin, ornitin dan arginin. Asam amino arginin dan ornitin menunjukkan hubungan yang signifikan dengan subtipe imunohistokimia. 

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Metabolomic approach to analyze the immunohistochemistry subtype in breast cancer is a promising tool, especially measuring amino acid levels. The amino acid profile on breast cancer patients has a significant role as guidance and prognosis. Previous studies showed alteration of amino acid levels in breast cancer patients compared to healthy women, indicating an association between amino acid and breast cancer. Some amino acids can be used as a biomarker for determining a relationship between breast cancer with cancer progression or immunohistochemistry. This study aims to investigate the association of amino with immunohistochemistry in breast cancer. This is a cross-sectional study that involved 51 breast cancer patients in RSUPN Cipto Mangunkusumo. A blood sample was collected from patients and analyze using High-Performance Liquid Chromatography (HPLC) methods to calculate the level of amino acid. The measurement of amino acid was compared to standard to determine amino acid alteration whether amino acid is increased or decrease. The data are analyzed and tested statistically to investigate the association of amino acid alteration based on three categories of immunohistochemistry (Luminal A, Luminal B+HER2 dan Triple-Negative Breast Cancer (TNBC)). A total of 51 breast cancer patients showed Luminal B+HER2 group has the highest frequency of immunohistochemistry subtype, followed by Luminal A and TNBC, respectively. There were mostly no changes in amino acid levels among the three subtypes, except arginine and histidine, which showed increased amino acid levels in Luminal A and Luminal B+HER2, whereas a decrease of ornithine level showed in TNBC group. Bivariate analysis showed significantly association between amino acid arginine and ornithine with immunohistochemistry subtype in breast cancer (p<0.05).  The majority of immunohistochemistry subtypes in breast cancer were Luminal B+HER2. Out of 19 amino acids, most of the amino acid are stable in three groups, excluding arginine, histidine and ornithine. Arginine dan ornithine showed a significantly association with immunohistochemistry subtype of breast cancer."

"Protein energy wasting (PEW) merupakan sindrom gangguan nutrisi yang sering terjadipada pasien penyakit ginjal kronik (PGK) dengan hemodialisis rutin sekitar 28-80%.Proses hemodialisis dapat meyebabkan hilangnya nutrien seperti asama amino,meningkatkan proses inflamasi yang kemudian dapat meningkatkan katabolisme protein,dan dapat menghambat utilisasi asam amino dalam sintesis protein. Jika tidak ditangani,PEW dapat meningkatkan morbiditas dan mortalitas pasien PGK. Tujuan utamapenelitian adalah untuk mengetahui profil asam amino pasien PGK dengan hemodialisisrutin. Desain penelitian adalah potong lintang dengan 60 subjek pasien PGK usia >18tahun dengan hemodialisis rutin di RS. Umum Pusat Nasional Dr. Cipto Mangukusumo.Sampel berupa dried blood spot (DBS) dan pemeriksaan asam amino menggunakanmetode Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Asam aminoyang diperiksa adalah asam amino nonesensial (alanin, arginin, asam aspartat, asamglutamat, asparagin, glisin, glutamin, prolin, serin, tirosin), esensial (histidin, fenilalanin,isoleusin, leusin, lisin, metionin, treonin, triptofan, valin), dan khusus (ornitin, sitrulin).Hasil penelitian didapatkan hampir semua kadar asam amino pada subjek lebih rendahterutama alanin, tirosin, histidin, dan valin; sebaliknya asam aspartat dan serin ditemukanlebih tinggi kadarnya dibandingkan nilai rujukan Mayo dan data internal dewasa sehat.Didapatkan adanya hubungan bermakna antara jenis kelamin dengan fenilalanin,isoleusin, leusin; hipoalbuminemia (albumin <4 g/dL) dengan glisin; hipoalbuminemia(<3,5 g/dL) dengan arginin, asam aspartat, asparagin, histidin, lisin, metionin, danornitin. Didapatkan korelasi yang bermakna antara usia dengan BCAA (isoleusin, leusin,valin), dan metionin; dan hemoglobin dengan isoleusin. Penelitian ini merupakanpenilitian pertama tentang profil asam amino pada pasien PGK dengan hemodialisis diIndonesia dan penelitian pertama kali yang menggunakan sampel DBS pada orangdewasa. Dengan diketahuinya profil asam amino pada PGK dapat dimanfaatkan sebagaidasar pemberian jenis suplementasi asam amino yang sesuai dengan populasi pasien PGKdengan hemodialisis di Indonesia.

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Protein energy wasting (PEW) is a nutritional disorder syndrome that often occurs in

patients with chronic kidney disease (CKD) on routine hemodialysis around 28-80%. The

process of hemodialysis can cause the loss of nutrients such as amino acids, increase the

inflammatory process which can increase protein catabolism, and be able to inhibit the

utilization of amino acids in protein synthesis. If untreated, PEW can increase the

morbidity and mortality of CKD patients. The main objective of the study was to

determine the amino acid profile of CKD patients on routine hemodialysis. The study

design was cross sectional with 60 subjects of CKD patients aged >18 years on routine

hemodialysis at Dr. Cipto Mangunkusumo National Public Hospital. Samples in the form

of dried blood spot (DBS) and amino acid examination using the Liquid Chromatography

Tandem Mass Spectrometry (LC-MS/MS) method. Amino acids examined were

nonessential amino acids (alanine, arginine, aspartic acid, glutamic acid, asparagine,

glycine, glutamine, proline, serine, tyrosine), essential (histidine, phenylalanine,

isoleucine, leucine, lysine, methionine, glycine, glutamine, proline, serine, tyrosine),

special (ornithine, citrulline). The results showed that almost all amino acid levels in the

subjects were lower especially alanine, tyrosine, histidine, and valine; in contrast, aspartic

acid and serine were found to be higher than Mayo reference value and internal data of

healthy adults. A significant relationship was found between gender and phenylalanine,

isoleucine, leucine; hypoalbuminemia (albumin <4g/dL) with glycine; hypoalbuminemia

(<3.5 g/dL) with arginine, aspartate acid, asparagine, histidine, lysine, methionine, and

ornithine. Significant correlation was obtained between age with BCAA (isoleucine,

leucine, valine), and methionine; and hemoglobin with isoleucine. This study is the first

study of the amino acid profile in CKD patients with hemodialysis in Indonesia and the

first study using DBS samples in adults. Knowing the amino acid profile in CKD can be

used as a basis for the of amino acid supplementation that is suitable for the population

of CKD patients with hemodialysis in Indonesia."

"Latar Belakang: Hitung limfosit total berhubungan dengan prognosis serta harapan hidup pasien kanker kepala leher. Regulasi limfosit dipengaruhi berbagai hal termasuk nutrisi. Salah satu zat gizi yang berperan dalam proliferasi limfosit adalah asam amino rantai cabang. Penelitian ini bertujuan untuk melihat korelasi antara asupan asam amino rantai cabang dengan hitung limfosit total pada pasien kanker kepala dan leher.Metode: Studi potong lintang ini dilakukan pada subjek dewasa dengan kanker kepala leher yang belum menjalani terapi di poliklinik radioterapi dan hematologi onkologi medik RSCM. Asupan asam amino rantai cabang dinilai dengan 3 x 24-h food recall dan FFQ semi kuantitatif. Hitung limfosit total diukur dengan differential blood cell counter.Hasil: Sebanyak 85 subjek penelitian dengan rerata usia 53 tahun, dengan sebagian besar laki-laki, terdiagnosis kanker nasofaring dengan jenis karsinoma sel skuamosa dan stadium IV. Rerata subjek memiliki status gizi normal, dengan rerata asupan energi 29,99 ± 0,95 kkal/kgBB dan protein 1,39 ± 0,05 g/kgBB dengan penilaian FFQ semi kuantitatif. Rerata asupan AARC pada subjek sebesar 10,92 ± 0,48 gram dengan FFQ semi kuantitatif. Sebagian besar subyek memiliki hitung limfosit total pada rentang normal. Terdapat sebanyak 17.6% subyek dengan hitung limfosit total yang rendah. Terdapat korelasi lemah antara asupan asam amino rantai cabang dengan hitung limfosit total (r=0,230, p=0,029).Kesimpulan: Terdapat korelasi bermakna yang lemah antara asupan AARC dengan hitung limfosit total pada subjek kanker kepala leher yang belum menjalani kemoradioterapi.

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Background: Total lymphocyte count is related with prognosis and survival rate of head and neck cancer patients. Lymphocyte regulation is affected by multiple factors, including nutrition. One of the nutrients that plays role in lymphocyte proliferation is branched-chain amino acids. This study aims to investigate the correlation between branched-chain amino acid and total lymphocyte count in head and cancer patients.

Method: This cross-sectional study was conducted on adults with head and neck cancer who had not undergone therapy at the radiotherapy and medical hematology oncology clinic at RSCM. Branched-chain amino acid intake was assessed using 3x24-h food recall and semi quantitative FFQ. Total lymphocyte count was measured with differential blood cell counter.

Results: Eighty-five subjects with a mean age of 53 years, mostly are male, diagnosed with nasopharyngeal cancer, with histopathology appearance of squamous cell carcinoma, and stage IV cancer. The average subject had normal nutritional status, with an average intake of 29.99 ± 0.95 kcal/kgBW of energy and 1.39 ± 0.05 g/kgBW of protein with a semi quantitative FFQ assessment. The average branched-chain amino acid intake in subjects was 10,92 ± 0,48 gram with semi quantitative FFQ. There were 17.6% subjects with low total lymphocyte count. There was a low correlation between intake of branched-chain amino acids and total lymphocyte count (r=0,230, p=0,029).

Conclusion: There was a significant low correlation between branched-chain amino acids intake with total lymphocyte count in head and neck cancer subjects who had not undergone chemoradiotherapy."

"ABSTRAKPasien kanker payudara di Indonesia sering mengalami ketidaknyamanan fisik dan psikologis, namun perawat belum dapat secara optimal membantunya. Hal ini terjadi antara lain karena sulitnya mengkaji kondisi/tingkat kenyamanan dan mengidentifikasi faktor-faktor yang mempengaruhi kenyamanannya. Penelitian ini bertujuan untuk mengembangkan instrumen pengkajian kenyamanan dan mengidentifikasi faktor-faktor yang mempengaruhi kenyamanan melalui suatu pemodelan teoritis kenyamanan dalam pendekatan Kolcaba. Penelitian ini diawali dengan studi literatur, wawancara mendalam dengan 10 pasien, dan konsultasikan dengan 10 orang pakar dari berbagai keilmuan terkait, dilanjutkan dengan menggunakan metode cross sectional untuk uji construct validity dan uji model teoritis dengan menggunakan Structural Equation Modelling pada 308 pasien kanker payudara. Hasil penelitian ini adalah instrumen pengkajian kenyamanan kanker payudara yang diberi nama ldquo;PKKP rdquo; dan pemodelan teoritis kenyamanan pada pasien kanker payudara di Indonesia dengan pendekatan Kolcaba. Hasil penelitian menjelaskan bahwa dukungan emosi, perawatan paliatif, spiritual, dan usia pasien mempengaruhi kenyamanan pasien melalui mediator kondisi fisik dan emosi. Pengembangan perawatan paliatif yang memperhatikan aspek psikologis, spiritual, dan karakteristik usia pasien, diperlukan di Indonesia untuk meningkatkan kenyamanan pada pasien kanker, khususnya pada pasien kanker payudara.

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ABSTRACTBreast cancer rsquo patients in Indonesia frequently experience physical and psychologist discomforts. Nevertheless, it is difficult for nurses, to precisely measure comforts and identify factors affecting comforts among cancer patients. This study aimed to develop a comfort assessment tool and generate a theoretical comfort model for breast cancer patients in Kolcaba approach. The first stage of this study used literature reviews, in depth interviews with 10 cancer patients, and consulted with 10 experts, followed by cross sectional method for construct validity test and theoretical model tests using Structural Equation Modelling to 308 breast cancer patients. Results of our study were the comfort assessment breast cancer instrument and a theoretical comfort model for breast cancer patients in Indonesia. The results of this research concluded emotional support, palliative care, spiritual, and patients rsquo age affects patients 39 comfort through the mediator of physical and emotional conditions. Our study suggested the development of palliative care that takes into account psychologist, spiritual and patient rsquo s characteristic aspect to promote comforts among breast cancer patients."

"ABSTRAKLatar belakang: Protein merupakan salah satu nutrisi penting dalam pertumbuhan yang kualitasnya dipengaruhi oleh asam amino pembentuknya. Asam amino merupakan bahan baku pembangun semua jenis sel, berperan dalam homeostasis, pertahanan tubuh, pertumbuhan, dan perkembangan. Penelitian ini merupakan penelitian pendahuluan untuk mendapatkan gambaran profil asam amino meliputi glisin, alanin, prolin, valin, leusin, ornitin, metionin, fenilalanin, arginin, sitrulin, tirosin, aspartat, dan glutamat menggunakan metode LC-MS/MS pada anak undenutrition dan anak normal di RSUPN Cipto Mangunkusumo. Metode: Desain penelitian adalah deskriptif analitik dengan 60 subjek, penelitian berlangsung pada bulan Desember 2016 sampai April 2017. Sampel menggunakan dry blood spot dan diperiksa dengan metode LC-MS/MS. Hasil: Hasil penelitian didapatkan 12 anak undernutrition dan 18 anak normal dengan rerata berat badan, tinggi badan dan ketiga z-score BB_TB, BB_U, dan TB_U didapatkan lebih rendah secara bermakna pada kelompok undernutrition. Hasil CV uji ketelitian within run asam amino dengan LC-MS/MS berkisar 1.76 ndash; 12.03 . Kesimpulan: Tidak terdapat perbedaan bermakna antara profil asam amino esensial anak undernutrition dan anak normal, namun didapatkan perbedaan untuk asam amino non esensial kadar glisin dan glutamat lebih tinggi pada kelompok undernutrition dan bermakna secara statistik.

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ABSTRACTBackground Protein is one of the nutrients needed for child rsquo s growth, of which quality is affected by its constituent amino acids. Amino acids are essential to all types of cells, playing a role in homeostasis, the body 39 s defenses, growth, and development. This study is a preliminary study that aims to determine the profile of amino acids consisting of glycine, alanine, proline, valine, leucyne, ornithine, methionine, phenylalanine, arginine, citruline, tyrosine, aspartic acid, and glutamic acid using LC MS MS method in normal and undernutrition child at RSUPN CM. Method This was a descriptive analitic study conducted on 60 subjects, the study was held on December 2016 until April 2017. Sample using dry blood spot and analyzed with LC MS MS method. Result Study subjects consisted of 12 undernutrition and 18 normal children with a mean weight, height, and all z score W H, W A, H A are lower in undernutrition group. Within run result demonstrated a CV amino acid with LC MS MS ranged from 1.76 ndash 12.03 . Conclusion There were no difference between normal child rsquo s essential amino acid profile with undernutrition child rsquo s, but there were difference for non essential amino acid glisine and glutamate gives a significantly higher result in undernutrition group. "

"Coronavirus Disease 2019 (COVID-19) disebabkan oleh infeksi virus SARS CoV-2, dan telah dilaporkan banyak menyebabkan kematian di berbagai negara. Pada pasien COVID-19, ditemukan perubahan kadar asam amino, baik asam amino esensial maupun non esensial, yang dikaitkan dengan proses inflamasi dan infeksi. Tujuan penelitian ini adalah mengetahui profil asam amino esensial dan non esensial, serta mengetahui perbedaannya pada pasien terkonfirmasi COVID-19 severe dan non severe. Penelitian dilakukan dengan metode potong lintang di RSUPN Dr. Cipto Mangunkusumo dan Laboratorium HGRC pada Januari-Desember 2021. Total subjek adalah 128 subjek, terdiri dari 70 subjek (54,7%) kelompok severe dan 58 subjek (45,3%) kelompok non severe. Profil asam amino pada pasien terkonfirmasi COVID-19 severe dan non-severe, secara klinis ditemukan sedikit perbedaan dengan rentang effect size d 0,08-0,48. Tidak terdapat perbedaan bermakna keseluruhan profil asam amino antara kelompok severe dan non severe (p>0,05). Temuan ini diharapkan dapat memberi kontribusi dalam proses penyembuhan pasien terutama pada kondisi infeksi/inflamasi akut, serta menurunkan angka morbiditas dan mortalitas melalui penambahan asupan makanan atau terapi suplementasi potensial pada penderita dengan kadar asam amino yang lebih rendah

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Coronavirus Disease 2019 (COVID-19) is an infection, caused by SARS CoV-2 virus infection, and had been reported that cause death in many countries. Patients with COVID-19 infection, could have amino acid alteration, both in essential and non essential, which are associated in inflammation and infectious processes. The main objective of this study, was to know the essential and non essential amino acid profile, and to determine the differences in severe and non severe COVID-19 patients. This cross sectional study was conducted at Dr. Cipto Mangunkusumo National Central Public Hospital and HGRC Laboratory, from January-December 2021. There were 128 subjects, consisted of 70 subjects (54.7%) in severe group and 58 subjects (45.3%) in non severe group. The amino acid profile in severe and non-severe COVID-19 patients, clinically were found slight different, with the effect size range d 0.08-0.48. There was no significant difference in all amino acid, between severe and non severe group (p>0.05). These findings were expected to contibute in recovery process especially in infection/acute inflammation state, decreased the morbidity and mortality, through additional intake and potential supplementation therapy in lower amino acid patients."

"ABSTRAKStunting merupakan kondisi malnutrisi pada anak yaitu tinggi badan menurut usia lebih dari minus 2 simpang baku. Indonesia menempati urutan kelima di dunia. Stunting berkorelasi dengan asupan makanan terutama protein, IGF-1 dan protein pengikat Insulin like Growth Factor Binding Protein IGFBP-3 , dan Zinc Zn . Kualitas protein dinilai dari profil asam amino bebas plasma Plasma Free Amino Acid = PFAA dan kuantitas dinilai dari jumlah asupan protein harian. Beberapa penelitian menemukan kadar IGF-1 dipengaruhi oleh polimorfisme SNP rs5742612, rs35767 dan rs35766. Penelitian ini bertujuan untuk menganalisis peran profil PFAA, IGF-1, IGFBP-3, polimorfisme IGF-1, Insulin, dan Zn pada anak.Penelitian ini merupakan studi comparative cross sectional dilakukan di RS Cipto Mangunkusumo, Lembaga Biomolekular Eijkman, dan Labkesda DKI Jakarta. Subjek penelitian adalah anak usia 1 ndash;3 tahun berasal dari UPTD Puskesmas Jatinegara dibagi menjadi 2 kelompok yaitu 101 anak stunted dan 101 anak nonstunted.Pada penelitian ini didapatkan kadar 8 dari 9 AA esensial, 2 AA esensial kondisional, dan 2 AA nonesensial lebih rendah bermakna kelompok stunted dibandingkan nonstunted. Profil PFAA yaitu jumlah anak di bawah nilai rujukan berbeda bermakna antara kelompok stunted dan nonstunted. Terdapat korelasi 8 AA esensial, 1 AA esensial kondisional, dan 2 AA nonesensial dengan tinggi badan anak. Pada kelompok anak stunted, IGF-1, IGFBP-3, insulin, Zn, energi total dan protein lebih rendah bermakna dari kelompok anak nonstunted. Terdapat korelasi bermakna AA esensial dengan IGF-1 dan IGFBP-3. Polimorfisme rs35766 genotipe AG kodominan memiliki pengaruh terhadap kadar IGF-1 pada kelompok nonstunted. Faktor yang memengaruhi kejadian stunting adalah energi atau protein, IGF-1 yang berinteraksi dengan genotipe kodominan AG, IGFBP-3, dan Zn.Simpulan: PFAA, IGF-1 yang berinteraksi dengan SNP rs35766 genotipe kodominan AG, memiliki pengaruh terhadap kejadian stunting. Perlu penelitian lebih lanjut mengenai pemberian pola makan yang tepat untuk mencegah dan mengatasi anak stunted. Kata kunci: AA esensial, AA nonesensial, IGFBP-3, insulin, PFAA, stunting, Zn

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ABSTRACTStunting is a malnourished condition in children defined by height for age is under minus 2 standard deviation. Indonesia ranked fifth in world for this condition. Stunting mainly corelates with low protein intakes, IGF-1 and its binding protein Insulin-like Growth Factor Binding Protein/IGFBP-3 , and zinc Zn . Plasma free amino acid profile PFAA measures quality of protein intake, whilst its quantity measured by daily protein intake records. Previous studies found IGF-1 level affected by single nucleotide polymorphism SNP on rs5742612, rs35767 and rs35766. This study aims to analyze the role of PFAA, IGF-1, IGFBP-3, and IGF-1 polymorphism, insulin, and Zn in children.This study is a comparative cross-sectional study held in Cipto Mangunkusumo National General Hospital, Eijkman Institute for Molecular Biology, and Jakarta Provincial Public Health Laboratory. Subjects were children age 1 ndash;3 years old from Jatinegara Region Public Health Centre divided into two groups of 101 stunted children and 101 non-stunted children.Eight essential AA levels, 2 conditional essential AAs, 2 nonessential AAs were significantly lower in stunted groups than non-stunted. There was significant difference of profile PFAA below normal range between stunted and non-stunted group. Eight essential amino acids, 1 conditional essential amino acid, and 2 non-essential amino acid correlate with children rsquo;s height. IGF-1, IGFBP-3, insulin, Zn, total energy, and protein were significantly lower in stunted children compare to non-stunted children. Significant correlations found for all essential amino acids with IGF-1 and IGFBP-3. The rs35766 AG codominant polymorphism affects IGF-1 level in non-stunted group. Factors affects stunting condition were total energy or protein intake, IGF-1 that interacts with AG codominant genotype, IGFBP-3, and Zn.Conclusion: PFAA and IGF-1 that interacts with SNP rs35766 AG codominant genotype affect stunting. Further study needed to determine appropriate dietary habit for stunting prevention and treatsment. Keywords: Essential amino acid, IGFBP-3, insulin, non-essential amino acid, plasma free amino acid profile, stunting, zinc"