Hasil Pencarian  ::  Simpan CSV :: Kembali

"Latar belakang: Peningkatan suhu tubuh ekstrim menyebabkan denaturasi protein,terhentinya reaksi enzimatik, hilangnya aktivitas dan integritas membran, sertamemicu terjadinya kerusakan sel. Peningkatan suhu tubuh juga mempengaruhiterjadinya efek inotropik dan kronotropik positif pada jantung. Diperkirakan bahwapajanan panas dapat meningkatkan ekspresi protein Transient Receptor PotentialVanilloid 1 (TRPV1), Heat Shock Factor 1 (HSF1) dan Heat Shock Protein 70(Hsp70) pada kardiomiosit berperan penting dalam proses termotoleran danaklimatisasi terhadap panas serta berguna sebagai mekanisme adaptasi secara sistemikdan seluler. Tujuan dari penelitian adalah untuk menganalisis ekspresi TRPV1, HSF1,dan Hsp70 pada jantung sebagai respons protektif terhadap pajanan panas.Metode: Penelitian bersifat eksperimental in vivo menggunakan hewan coba tikusjenis Sprague Dawley (SD) berumur 12 minggu dengan berat badan 200-300 gram dilaboratorium hewan Balitbangkes Kemenkes RI, pada Oktober-Desember 2014.Sebanyak 28 ekor tikus jantan dengan n=4 pada tiap kelompoknya, dibagi dalamkelompok Kontrol (K) dan kelompok Perlakuan (P). Kelompok perlakuan terdiri dari6 subkelompok (kelompok hari ke-1,3,7,10,14 dan 21) mendapatkan pajanan panas didalam hyperthermic chamber bersuhu (45oC ± 0.3oC) dan kelembaban relatif (70% ±3%) selama 60 menit. Dilakukan pengukuran berat badan, suhu kulit, suhu rektal danfrekuensi denyut jantung. Perubahan morfologi kardiomiosit diamati menggunakanpewarnaan Hematoksilin-Eosin. Ekspresi TRPV1, HSF1 dan Hsp70 diperiksamenggunakan metode imunohistokimia dan ELISA.Hasil: Penelitian menunjukan pajanan panas 45oC; kelembaban relative 70% selama60 menit menyebabkan penurunan berat badan sejak hari ke-1 hingga hari ke-21perlakuan. Terjadi peningkatan suhu kulit, suhu rektal dan heart rate yang puncaknyaterjadi pada hari ke-7, dan menurun mulai pada hari ke-10 sampai pada hari ke-21meskipun intensitas pajanan panas tetap sama. Hal tersebut menandakan mekanismeaklimatisasi dan proses termotoleransi telah terjadi pada hari ke-7 perlakuan. Terjadipenambahan ukuran lebar kardiomiosit dan peningkatan berat pada jantung seiringlamanya pajanan panas, Hasil ini menunjukkan terjadinya hipertrofi jantung namuntidak disertai adanya fibrosis. Secara molekuler melalui pemeriksaan Imunohistokimiadan ELISA pada kardiomiosit menunjukkan ekspresi TRPV1, HSF1 dan Hsp70 yangbersifat sebagai protein protektif dan kardioprotektor cenderung mengalamipeningkatan sejak hari ke-1 sampai pada hari ke-7 perlakuan dan cenderung menurunpada hari ke-10 sampai dengan hari ke-21. Perubahan kadar ekspresi TRPV1, HSF1dan Hsp70 sejalan dengan perubahan yang terjadi pada suhu kulit, suhu rektal danheart rate.Kesimpulan: Pajanan panas pada tubuh memberikan pengaruh pada jantung berupaterjadinya hipertrofi konsentris disertai adanya peningkatan ekspresi TRPV1, HSF1dan Hsp70 yang berperan penting sebagai protein protektif dan kardioprotektorBackground: Increased extreme body temperature causes protein denaturation,cessation of enzymatic reactions, loss of membrane activity and integrity, and triggerscellular damage. Increased body temperature also affects the occurrence of positiveinotropic and chronotropic effects on the heart. It is postulated that increase inexpression Transient Receptor Potential Vanilloid 1 (TRPV1), Heat Shock Factor 1(HSF1), Heat Shock Protein 70 (Hsp70) in cardiomyocytes is activated by extremetemperatures and has an important role in thermotolerance and heat acclimatizationprocesses -and as a mechanism of systemic and cellular adaptation. The aim of thestudy was to analyze the expression of TRPV1, HSF1, and Hsp70 on cardiac muscleas a protective response to heat exposure.Methods: This in vivo experimental research was conducted using Sprague-Dawley(SD) rats (age 12 weeks, 200-300 gram) in animal laboratory National Institute ofHealth Research and Development, Indonesian Ministry of Health, October-December2014. A total of 28 male rats with n = 4 in each group, divided into Control group (K)and Treatment group (P). The treatment group consisted of 6 sub-groups (i.e.1,3,7,10,14 and 21 days) received heat exposure in hyperthermic chamber at (45oC ±0.3oC) and (70% ± 3%) with relative humidity of 60 minutes. Body weight, skintemperature, rectal temperature and heart rate were measured. Changes incardiomyocyte morphology were observed using Hematoxylin-Eosin staining.Expressions of TRPV1, HSF1 and Hsp70 were examined using immunohistochemicaland ELISA methods.Results: The results of this study showed that heat exposure at 45oC;70% RH for 60minutes resulted in weight loss from day 1st to day 21st of the treatment. Peakselevation in skin temperature, rectal temperature and heart rate were reached at day7th, and decreased gradually from day 10th to day 21st even though the intensity ofheat exposure was unchanged. This indicated the mechanism of acclimatization andthermotolerance process had occurred on the 7th day of heat treatment. There wasincreased in the size of the cardiomyocyte width and heart weight along with theduration of heat exposure. These results indicated the occurrence of heart hypertrophybut not accompanied by fibrosis. Molecular aspects on cardiomyocytes throughImmunohistochemistry and ELISA showed TRPV1, HSF1 and Hsp70 expression asprotective proteins and cardioprotectors, which tended to increase from day 1st to 7thday of treatment and decrease gradually on day 10th to day 21st. Changes inexpression levels of TRPV1, HSF1 and Hsp70 coincided with changes in skintemperature, rectal temperature and heart rate.Conclusion: Heat exposure to the body induced the development of heart hypertrophyand coincided with the increased expression of TRPV1, HSF1 and Hsp70 which act asa protective protein and cardioprotector."

"Olahraga aerobik terbukti mampu meningkatkan struktur dan fungsi kognitif. Data tentang jenis olahraga aerobik yang terbaik untuk menjaga keseimbangan oksidatif, serta memicu angiogenesis dan neuroplastisitas di berbagai regio otak masih terbatas. Penelitian ini menganalisis pengaruh olahraga aerobik halang rintang terhadap kadar neuroglobin otak, protein pemicu angiogenesis, neuroplastisitas di hipokampus dan korteks prefrontal, serta fungsi memori relasional. Mencit Mus musculus CBS-Swiss strain jantan berusia 10 bulan dilatih berlari di roda berjalan yang diberi halang rintang, berlari dengan kecepatan 10 m/menit, 30 menit/hari, 5 hari/minggu sambil melewati halang rintang untuk setiap 78 cm. Tiga jenis halang rintang diganti setiap 3 hari. Kelompok pembanding adalah mencit yang berlari dengan kecepatan dan waktu yang sama, namun tanpa halang rintang, serta kelompok kontrol yang tidak berolahraga. Kadar neuroglobin otak tidak berbeda bermakna pada tiga kelompok mencit. Latihan lari halang rintang memberikan efek lebih baik dibanding lari tanpa halang rintang pada kadar developmentally regulated brain protein-A (drebrin-A) di hipokampus. Kedua kelompok olahraga memiliki efek yang lebih baik dibanding kontrol pada ekspresi vascular endothelial growth factors (VEGF), kadar drebrin-A, dan paired associative cognitive test. Olahraga aerobik kompleks memicu neuroplastisitas yang lebih baik dibanding aerobik sederhana di hipokampus. Kedua tipe olahraga aerobik mampu meningkatkan angiogenesis dan neuroplastisitas di otak, dan meningkatkan kemampuan memori relasional. Olahraga aerobik tidak meningkatkan kadar neuroglobin secara bermakna. Hal ini menunjukkan bahwa intensitas olahraga aerobik memberikan efek hipoksia yang dapat ditoleransi oleh jaringan otak.

---

Aerobic exercise has been proven to improve of cognitive structure and function. Study about the type of aerobic exercise affects on angiogenesis, neuroplasticity and oxidative homeostasis in brain?s specific regions is still limited. The present study was conducted to investigate the effects of hurdle aerobic exercise on brain neuroglobin level, angiogenesis and neuroplasticity proteins in hippocampus and prefrontal cortex, and relational memory among middle aged CBS-Swiss strain mice. Mice, age 10 months were subjected to hurdle running wheel for 8 weeks. They ran at speed of 10 m/min, 30 min/day, and 5 days/week with hurdles for every 78 cm. Three types of hurdles were changed for every 3 days. Another group of same age mice ran at same speed, time, and period, without hurdle as comparison, while other control group never exercises. The hurdle group exercise has significant higher level of developmentally regulated brain protein-A (drebrin- A) in hippocampus compared to non-hurdle group. Both of exercise groups have significant higher ability on paired associative cognitive test, and they have significant higher expression of vascular endothelial growth factor (VEGF) and higher level of drebrin-A compared to control. Neuroglobin level was not significant different among of all groups. More complex aerobic exercise has better effect on hippocampus neuroplasticity. Both of aerobic exercise has better effect on angiogenesis and neuroplasticity in the brain, and also on cognitive function. Aerobic exercise does not resulting high hypoxic stress and could be tolerated by brain."

"ABSTRAKOverweight dan obesitas adalah akumulasi lemak yang berlebihan yang dapat mengganggu kesehatan. Obesitas merupakan salah satu risiko untuk terjadinya gangguan kardiometabolik. Adanya polimorfisme gen UCP1, menyebabkan bervariasinya respons terhadap olahraga teratur SALI.Penelitian ini bertujuan menganalisis pengaruh senam aerobik low impact SALI pada perempuan obes abdominal yang memiliki polimorfisme gen UCP1 terhadap parameter kardiometabolik lingkar pinggang LP , kadar trigliserida TG dan penanda inflamasi Monocyte Chemmoattractant Protein-1 MCP1 .Desain penelitian adalah non randomized controlled trial. Intervensi 12 minggu terhadap 55 orang wanita obes abdominal, terdiri dari 32 orang kelompok intervensi SALI dan 23 orang kelompok kontrol. Sebelum dan sesudah program dilakukan pemeriksaan parameter kardiometabolik LP, kadar TG dan MCP1. Pemeriksaan polimorfisme -3826 A>G gen UCP1 menggunakan teknik PCR diikuti teknik RFLP.Ditemukan frekuensi genotip AA 21 orang 38,2 , genotip AG 27 49,1 dan genotip GG 7 12,7 , dengan frekuensi alel G 0,40 . Subjek dengan kadar TG ge; 130 mg/dL kelompok SALI 100 responders, Kontrol 55 , dan kelompok kadar TG < 130 mg/dL, 22 . Subjek genotip GG polimorfisme gen UCP1 dengan kadar TG ge; 130 mg/dL high responders. genotip AA low responders. Temuan ini diperkirakan terkait jumlah langkah per hari kelompok genotip GG dengan TG ge; 130 mg/dL lebih tinggi dibandingkan kelompok nonresponders.

---

ABSTRACTOverweight and obesity were the accumulation of excessive fat that could harm health. Obesity was a risk for cardiometabolic disorders. The polymorhism of UCP1 gene, caused variations in response to regular exercise. This study aims to investigate the influence of low impact aerobics exercise LIAE in abdominal obes women who had the polymorphism of UCP1 gene on cardiometabolic parameters, waist circumference WC , levels of triglyceride TG and inflammatory markers Monocyte Chemoattractant Protein 1 MCP1 . The study design was non randomized controlled trial. A total of 55 women subjects moderately obes women were divided into two groups on the basis of location. Thirty two subjects of LIAE group and 23 a non LIAE control group. Subjects were not restricted in foods consumed. The study period was 12 weeks. Outcome assessments for analyses were completed at baseline and 12 weeks for cardiometabolic parameters WC, TG and MCP1. Examination of the polymorphism 3826 A G UCP1 gene using PCR technique followed by RFLP technique. The frequencies of three genotypes of 3826 A G polymorphism of UCP1 gene were AA, AG, and GG were of 21 38.2 , 27 49.1 and 7 12.7 respectively with the G allele frequency of 0.40 . Post study obtained the subjects with TG baseline TG ge 130 mg dL 100 LIAE responders group, Control 55 , while TG "

"ABSTRAKPada perempuan pascamenopause diperlukan marka biokimiawi dan klinis pada masa jendela terapeutik yang diharapkan dapat digunakan untuk menapis HKND yang berperan sangat penting dalam menghindari dampak demensia tipe alzheimer setelah terapi hormon. Penapisan HKND dapat dimanfaatkan juga untuk upaya terapeutik HKND pada perempuan pascamenopause dan mencegah perburukan ke dalam kondisi demensia tipe alzheimer. Dilakukan studi potong lintang pada 282 perempuan pascamenopause di Jakarta yang dibagi menjadi 2 kelompok, yaitu kelompok positif dengan HKND dan kelompok tanpa HKND. Pemeriksaan dilakukan pada sejumlah variabel seperti usia, lama menopause, keluhan vasomotor, IMT, kadar FSH, kadar LH, kadar leptin, kadar estradiol, dan status kognitif, kemudian dianalisis secara statistik. Diperoleh nilai FSH yang berhubungan bermakna dengan kejadian HKND p = 0,018 , serta variabel lain seperti nisbah FSH/estradiol p = 0,029 dan nisbah FSH/sOB-R p = 0,011 , sementara variabel lain tidak bermakna. Analisis multivariat menunjukkan nisbah FSH/estradiol adalah variabel yang paling berperan terhadap kejadian HKND, dengan nilai OR 1,15. Berdasarkan kurva ROC didapat nilai titik potong nisbah FSH/estradiol dalam memprediksi HKND adalah 1,94 dengan sensitivitas 66,5 dan spesifisitas 46,8 . Nisbah FSH/estradiol pada perempuan pascamenopause yang menderita HKND yang lebih tinggi secara bermakna dibandingkan dengan kelompok tanpa HKND dikaitkan dengan peran neuron KNDy terhadap peningkatan sekresi GnRH dan rendahnya neurosteroid estradiol di otak perempuan pascamenopause yang berisiko menderita HKND. Nilai nisbah FSH/estradiol > 1,94 dapat digunakan untuk penapis diagnostik HKND pada perempuan pascamenopause.

---

ABSTRACTBiochemical and clinically important markers are needed in the window therapeutic period for postmenopausal women which are expected to be used as a screening methods for CIND as it is very important in avoiding the effect of dementia associated Alzheimer disease after hormone therapy. CIND screening was also useful for CIND treatment strategies in postmenopausal women and preventing postmenopausal women from impaired cognitive function due to dementia. A cross sectional study included 282 postmenopausal women in Jakarta was done, and subjects were further classified into two groups, with CIND and without CIND. Several related variables such as age, duration of menopause, vasomotor symptoms, BMI, FSH level, LH level, leptin level, estradiol level, and cognitive status, were assessed and analyzed statistically. The prevalence of CIND was significantly correlated with FSH level p 0.018 , along with ratio of FSH levels estradiol p 0.029 and ratio of FSH sOB R p 0.011 , while other variables were not. By multivariate analysis, FSH estradiol ratio of 1.15 was found as the most significant factor with probability of having CIND in postmenopausal women. Using the ROC curve, the ratio threshold of FSH estradiol to predict CIND was 1.94, with sensitivity 66.5 and specificity 46.8 . Level of FSH estradiol ratio in postmenopausal women with CIND was significantly higher than women without CIND, and is related to the role of KNDy neurons that induce the secretion of GnRH, and low level of neurosteroid estradiol in postmenopausal women rsquo s brain with risk of CIND. Ratio of FSH estradiol levels 1.94 could be used for screening methods of CIND in postmenopausal women."