Hasil Pencarian  ::  Simpan CSV :: Kembali

"Pembentukan 8-Hidroksi-2′-Deoksiguanosin (8-OHdG) dalam Calf thymus DNA yang Disebabkan dari Reaksi 2′-Deoksiguanosin dengan Senyawa Propil Galat dan 2,6-Di-Tert-Butil-p-Benzoquinon secara In Vitro. Kerusakan oksidatif DNA yang disebabkan oleh propil galat (PG) dan 2,6-di-tert-butil-p-benzoquinon (BHT-quinon, metabolit BHT), dianalisis dari pembentukan DNA adduct, 8-hidroksi-2’-deoksiguanosin (8-OHdG), terhadap calf thymus DNA dan basa tunggal DNA, 2′-deoksiguanosin (dG) secara in vitro. PG dengan dimediasi oleh CuCl2 menyebabkan peningkatan 8-OHdG terhadap calf thymus DNA sebesar 9,17 kali lebih besar dibandingkan terhadap kontrol (DNA tanpa perlakuan). Dengan adanya CuCl2 pada konsentrasi 1,28 x10-5 M, rasio pembentukan 8-OHdG dari hasil interaksi antara dG dengan PG pada berbagai variasi konsentrasi (20-150 ppm) berkisar antara 75,50-312,06 8-OHdG terhadap 105 dG. Pembentukan 8-OHdG tersebut, meningkat dengan bertambahnya konsentrasi PG dari 20-80 ppm, kemudian mulai stabil dengan bertambahnya konsentrasi PG diatas 80 ppm. Sementara itu, BHT-quinon dengan adanya CuCl2 menyebabkan peningkatan 8-OHdG terhadap Calf thymus DNA sebesar 0,05 kali dibandingkan kontrol (DNA tanpa perlakuan). Analisis menggunakan LC-MS/MS dilakukan untuk mengidentifikasi 8-OHdG, dengan puncak induk (M+. + 1) 284 dan memiliki dua fragmen utama m/z 167,9 dan m/z 139,9.

---

Oxidative DNA damage caused by propyl gallate (PG) and 2,6-di-tert-butyl-p-benzoquinone (BHT-quinone, a metabolite of butylated hydroxytoluene (BHT)) was analyzed from the 8-hydroxy-2′-deoxyguanosine (8-OHdG) formation in calf thymus DNA and DNA base, 2′-deoxyguanosine (dG). PG in the presence of CuCl2 increased the 8-OHdG formation in calf thymus DNA by around 9.17 times as compared to the control (untreated DNA). In the presence of CuCl2 at 1.28×10-5 M, the 8-OHdG per dG ratio resulting from the reaction of dG with PG at various concentrations (20-150 ppm) ranged from 75.50 to 312.06 8-OHdG per 105 dG. The 8-OHdG formation increased when the PG concentration was increased from 20 ppm to 80 ppm, and then, it began to plateau around 80 ppm. On the other hand, BHT-quinone increased the formation of 8-OHdG in the presence of CuCl2 by 0.05 times as compared to the control (untreated DNA). LC-MS/MS analysis was used to identify the molecular structure of 8-OHdG, which had a base peak (M+. + 1) at m/z = 284 and two main fragments at m/z = 167.9 and m/z = 139.9."

"Konsumsi Energi Saat Laktasi dan Durasi Menyusui di Wilayah Kerja Puskesmas Margajaya Kota Bekasi Tahun 2014. Kendati rekomendasi angka kecukupan gizi menunjukkan lebih tingginya kebutuhan energy ibu saat laktasi dibandingkan saat hamil namun penelitian di Jakarta dan Depok menunjukkan konsumsi energy ibu laktasi justru lebih rendah. Penelitian ini bertujuan untuk mengetahui faktor yang berhubungan dengan konsumsi energi ibu laktasi serta hubungan antara konsumsi energy ibu laktasi dengan durasi menyusui di Puskesmas Margajaya Kota Bekasi tahun 2014. Penelitian dilakukan secara cross sectional terhadap 60 orang ibu menggunakan kuesioner termasuk Semi- quantitative Food Frequency Questionnaire. Analisis dilakukan menggunakan uji chi-square. Hasil penelitian menunjukkan adanya hubungan bermakna antara umur ibu, paritas, dan konsumsi energy ibu hamil dengan konsumsi energi ibu menyusui. Ibu cukup umur (>27 tahun), multipara dan konsumsi energy hamil rendah (<2.100 Kal/hari) berisiko untuk memiliki konsumsi energy laktasi yang rendah. Selain itu, ditemukan hubungan bermakna antara konsumsi energy ibu laktasi dengan durasi menyusui. Ibu yang konsumsi energinya saat laktasi rendah berpeluang 4 kali lebih besar untuk memiliki durasi menyusui yang singkat. Direkomendasikan perlunya informasi kepada ibu tentang pentingnya konsumsi energi saat laktasi karena kebutuhannya yang tinggi untuk mendukung produksi ASI.Disarankan juga agar peningkatan rekomendasi angka kecukupan energi untuk ibu laktasi bulan ke 6-12 dimajukan menjadi bulan ke-4 mengingat kebutuhan gizi memasuki bulan ke-4 tersebut sudah sangat besar.;Despite recommendation for higher energy intake during lactation than during pregnancy, researches at Jakarta and Depok showed that energy consumption during lactation was lower than during pregnancy. The purpose of this study is to investigate the association between individual characteristics and energy consumption during lactation, and to assess the relationship between energy consumption during lactation to duration of breastfeeding among 60 mothers in the working area of PuskesmasMargajayaBekasi City in 2014. This research used a cross-sectional design; data was collected through primary data collection by questionnaire and Semi-quantitative Food Frequency Questionnaire. Analysis was conducted using chi-square technique. The study found that mothers of sufficient age (>27 years old), multiparous, and had low (<2,100 kcal/day) energy intake during pregnancy had significant higher risk to low energy consumption during lactation. Mothers with low energy consumption during lactation had 4 times higher risk of short duration of breastfeeding. It is recommended to provide information on the importance of higher energy intake during lactation due to the higher need to support breastmilk production and also to shift forward the nutrition recommendation regarding additional energy intake during lactation period from month 6 to month 4 due to higher energy requirement that commences when lactating mothers enter the fourth month of lactation.

---

Despite recommendation for higher energy intake during lactation than during pregnancy, researches at Jakarta and Depok showed that energy consumption during lactation was lower than during pregnancy. The purpose of this study is to investigate the association between individual characteristics and energy consumption during lactation, and to assess the relationship between energy consumption during lactation to duration of breastfeeding among 60 mothers in the working area of PuskesmasMargajayaBekasi City in 2014. This research used a cross-sectional design; data was collected through primary data collection by questionnaire and Semi-quantitative Food Frequency Questionnaire. Analysis was conducted using chi-square technique. The study found that mothers of sufficient age (>27 years old), multiparous, and had low (<2,100 kcal/day) energy intake during pregnancy had significant higher risk to low energy consumption during lactation. Mothers with low energy consumption during lactation had 4 times higher risk of short duration of breastfeeding. It is recommended to provide information on the importance of higher energy intake during lactation due to the higher need to support breastmilk production and also to shift forward the nutrition recommendation regarding additional energy intake during lactation period from month 6 to month 4 due to higher energy requirement that commences when lactating mothers enter the fourth month of lactation."

"Despite recommendation for higher energy intake during lactation than during pregnancy, researches at Jakarta andDepok showed that energy consumption during lactation was lower than during pregnancy. The purpose of this study isto investigate the association between individual characteristics and energy consumption during lactation, and to assessthe relationship between energy consumption during lactation to duration of breastfeeding among 60 mothers in theworking area of PuskesmasMargajayaBekasi City in 2014. This research used a cross-sectional design; data wascollected through primary data collection by questionnaire and Semi-quantitative Food Frequency Questionnaire.Analysis was conducted using chi-square technique. The study found that mothers of sufficient age (>27 years old),multiparous, and had low (<2,100 kcal/day) energy intake during pregnancy had significant higher risk to low energyconsumption during lactation. Mothers with low energy consumption during lactation had 4 times higher risk of shortduration of breastfeeding. It is recommended to provide information on the importance of higher energy intake duringlactation due to the higher need to support breastmilk production and also to shift forward the nutrition recommendationregarding additional energy intake during lactation period from month 6 to month 4 due to higher energy requirementthat commences when lactating mothers enter the fourth month of lactation.Konsumsi Energi Saat Laktasi dan Durasi Menyusui di Wilayah Kerja Puskesmas Margajaya Kota BekasiTahun 2014. Kendati rekomendasi angka kecukupan gizi menunjukkan lebih tingginya kebutuhan energy ibu saatlaktasi dibandingkan saat hamil namun penelitian di Jakarta dan Depok menunjukkan konsumsi energy ibu laktasi justrulebih rendah. Penelitian ini bertujuan untuk mengetahui faktor yang berhubungan dengan konsumsi energi ibu laktasiserta hubungan antara konsumsi energy ibu laktasi dengan durasi menyusui di Puskesmas Margajaya Kota Bekasi tahun2014. Penelitian dilakukan secara cross sectional terhadap 60 orang ibu menggunakan kuesioner termasuk SemiquantitativeFood Frequency Questionnaire. Analisis dilakukan menggunakan uji chi-square. Hasil penelitianmenunjukkan adanya hubungan bermakna antara umur ibu, paritas, dan konsumsi energy ibu hamil dengan konsumsienergi ibu menyusui. Ibu cukup umur (>27 tahun), multipara dan konsumsi energy hamil rendah (<2.100 Kal/hari)berisiko untuk memiliki konsumsi energy laktasi yang rendah. Selain itu, ditemukan hubungan bermakna antarakonsumsi energy ibu laktasi dengan durasi menyusui. Ibu yang konsumsi energinya saat laktasi rendah berpeluang 4kali lebih besar untuk memiliki durasi menyusui yang singkat. Direkomendasikan perlunya informasi kepada ibutentang pentingnya konsumsi energi saat laktasi karena kebutuhannya yang tinggi untuk mendukung produksiASI.Disarankan juga agar peningkatan rekomendasi angka kecukupan energi untuk ibu laktasi bulan ke 6-12 dimajukanmenjadi bulan ke-4 mengingat kebutuhan gizi memasuki bulan ke-4 tersebut sudah sangat besar."