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"Penelitian in vitro ini bertujuan untuk mengetahui variasi morfologi gigi insisif, caninus, premolar dan molar pada penampang melintang 1/3 servikal, 1/3 tengah dan 1/3 apikal akar.Metode: Penelitian dengan desain observasi deskriptif ini menggunakan 128 gigi yang telah dicabut. Sampel ditandai dengan spidol permanen pada 1/3 servikal (garis batas CEJ), 1/3 tengah dan 1/3 apikal akar. Selanjutnya dilakukan pemotongan secara melintang menggunakan diamond disc dengan low speed straight hand piece pada 1/3 servikal. Saluran akar dipreparasi dengan file kemudian diirigasi dengan larutan NaOCl 2,5%. Setelah saluran akar bersih, dilanjutkan dengan pemotongan secara melintang pada 1/3 tengah dan 1/3 apikal akar. Penampang melintang yang mempunyai 2 saluran akar dipisahkan untuk kemudian dideteksi adanya isthmus dengan pengaplikasian methylene blue. Pengamatan dilakukan dengan stereomicroscope perbesaran 10x dengan digital camera.Hasil: 100% gigi anterior hanya memiliki 1 saluran akar dengan variasi bentuk oval, long oval dan bulat pada penampang melintangnya. 80% P1 atas memilki 2 saluran akar dan 100% gigi premolar bawah memiliki 1 saluran akar dengan dominasi bentuk oval, long oval dan bulat pada penampang melintangnya. Gigi molar mayoritas memiliki 3 saluran akar dan bentuk saluran akar bervariasi mulai dari oval, long oval, bulat dan flat/pipih. Isthmus ditemukan pada gigi P1 atas, P bawah, akar mesiobukal M1 atas, dan akar mesial molar bawah dengan berbagai tipe. Pada gigi molar 2 bawah ditemukan saluran akar berbentuk C (c-shaped) walaupun sangat sulit membedakan c-shaped dengan menyatunya 2 atau 3 saluran akar akibat berfusinya akar.Kesimpulan: Berbagai variasi morfologi saluran akar yang ditemukan pada potongan melintang 1/3 servikal, 1/3 tengah dan 1/3 apikal akar harus menjadi pertimbangan dalam melakukan perawatan saluran akar.

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The purpose of this study was to assess the root canal morphological variations of insisive, caninus, premolar and molar using the cross section method.Methods: 128 extracted teeth were collected and stored in NaCl solution. The teeth were equally divided into a coronal, middle, and apical third. To divide it, the exact lengths of the root canals were determined. The root were resected using diamond disc with low speed straight hand piece. The resected root surface were polished, rinse and dried. The cross-sectional root surfaces that have two canals stained with methylene blue to investigate root canal isthmus classification. All the cross-sectional root surface examine using a stereomicroscope with digital camera.Results: 100% of the anterior teeth demonstrated a single canal, 80% of maxillary first premolar demonstrated two canals and 100% of mandibular molar had single canal. The shaped of canal was varied from oval, long oval or round in the coronal, middle and apical third. The molar teeth showed a high incidence of three root canals and the shaped more varied from oval, long oval, round or flat in the coronal, middle, and apical third. Isthmus frequently presence between two root canals within one root. It can observed in the maxillary first premolar, mandibular premolar, mesiobuccal roots of maxillary first molar and mesial roots of mandibular molar with different type. C-shaped mostly found in mandibular second molar. It may be difficult to distinguish between a C-shaped canal and a mandibular second molar with single or three canals joining apically.Conclussion: These root canal morphological variations should be considered during root canal treatment."

"Latar Belakang: Kebocoran mikro dipengaruhi oleh jenis semen saluran akar. Tujuan: menganalisis tingkat kebocoran mikro pengisian saluran akar menggunakan semen resin epoksi (SRE) dan Mineral Trioxide Aggregate (SMTA). Metode: Tiga puluh dua gigi premolar bawah, dibagi dua kelompok sama besar, yaitu kelompok SRE dan SMTA. Setelah pengisian saluran akar, sampel diinkubasi (370C, 24 jam), kemudian direndam dalam tinta India selama 7 X 24 jam. Sampel didekalsifikasi sampai dengan transparan. Kedalaman penetrasi tinta dievaluasi dengan mikroskop stereo. Skor 1 untuk penetrasi tinta 0-0,5 mm, skor 2 untuk penetrasi tinta 0,51-1 mm, dan skor 3 untuk penetrasi tinta >1 mm. Hasil: Distribusi proporsi kebocoran terbesar kelompok SRE terdapat pada skor 1, yaitu sebesar 37,5%. Sedangkan distribusi proporsi kebocoran terbesar kelompok SMTA terdapat pada skor 1, yaitu sebesar 21,9%. Tidak terdapat perbedaan bermakna antara kelompok SRE dan SMTA. Kesimpulan: Semen resin epoksi dan semen MTA memiliki tingkat kebocoran yang sama.

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Background: The microleakage affected by type of root canal sealer.

Purpose: to analyze the microleakage of obturation using epoxy resin-based (SRE) and mineral trioxide aggregate-based (SMTA) as root canal sealer.

Methods: Thirty two mandibular first premolars were equally divided into two groups. They were SRE group and SMTA group. After obturation, the specimens were incubated (370C, 24 h), immersed in Indian ink for 7 days, decalcified, dehydrated, and made transparent. Dye penetration were evaluated under stereomicroscope and given score 1-3. Specimen with 0-0,5 mm dye penetration was given score 1, while 0,51-1 mm penetration was given score 2, and > 1 mm was given score 3. The results were statistically analyzed with Kolmogorov Smirnov test.

Results: The largest proportion distribution in SMTA group was score 1 (37,5%), whilst the largest proportion distribution in SMTA group was score 1 (21,9%). There was no significant difference between the microleakage of epoxy resin-based and mineral trioxide aggregate-based sealer, observed from the one-third apical leakage.

Conclusion: The microleakage of mineral trioxide aggregate based sealer and epoxy resin-based sealer was relatively similar."

"Pada tahap pengerasannya, material bioaktif akan mengalami mekanisme hidrasi dengan melepaskan beberapa ion yang dikandungnya. Reaksi pada permukaan material ini dapat melepaskan dan merubah konsentrasi dari ion-ion terlarut yang akan memicu terjadinya respon intraseluler dan ekstraseluler dan akan mengkonduksi terjadinya pembentukan jaringan keras. Ion kalsium (Ca2+) yang dilepas material bioaktif berperan dalam fungsinya sebagai peningkat pH, bakterisid, menekan aktivitas osteoklas, serta merangsang pembentukan fibroblas.Tujuan dari penelitian ini adalah untuk menganalisis pelepasan ion Ca2+ dari material bioaktif MTA-Angelus® dan Biodentine®. Sebanyak 46 sampel dipersiapkan dengan ukuran Ø 2 mm dan tinggi 2 mm, terdiri dari 23 sampel kelompok MTA Angelus®, dan 23 sampel kelompok Biodentine® direndam dalam air deionisasi selama 1 jam dan 48 jam. Larutan perendam kemudian diukur kadar pelepasan ion Ca2+-nya menggunakan alat atom absorption sphectropometer, kemudian hasilnya diuji statistik menggunakan uji Kruskal Wallis. Hasil uji statistik post hoc Mann Whitney menunjukkan adanya perbedaan yang bermakna diantara semua kelompok dengan nilai signifikansi p≤0,05. Biodentine® terbukti melepaskan ion Ca2+ lebih banyak dibandingkan dengan MTA Angelus® pada waktu pengukuran 1 jam dan 48 jam.MTA Angelus® melepaskan ion Ca2+ lebih cepat jika dibandingkan dengan Biodentine®.

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On the setting stage, the bioactive materials will experience hydration mechanism by releasing a number of their ions. The reaction on the surface of these materials can release and alter dissolved ions concentration which will trigger an intracellular and extracellular responses. This process will also conduct remineralization. The released Ca2+ ions will increase alkalinizing activity, antibacterial, suppressing osteoclast activity as well as stimulating fibroblast formation. The aim of this study is to analyze Ca2+ ion release from MTA Angelus® dan Biodentine® as a bioactive material. As many as 46 samples are prepare with the size of 2 mm in diameters and 2 mm in height. The samples consist of 23 of MTA Angelus® samples, and 23 of Biodentine® samples. Both materials were soaked in deionized water for an hour which will then be measured. Both materials will then be transferred into fresh solution and will be soaked for 48 hours before they would be measured for the second time. The measurements will be conducted by using atom absorption sphectropometer. The result will later be statistically tested using a Kruskal Wallis test. Mann Whitney post hoc’s statistic test result showed a significant discrepancy among all groups, whit the significant value of p≤0,05. . Biodentine® was proven to release more Ca2+ ions compared to MTA Angelus® during the 1 and 48-hour measurments. MTA Angelus® released Ca2+ ion faster than Biodentine® does."

"Resin komposit merupakan material tumpat yang sensitif dan membutuhkan area kerja yang kering. Penelitian ini bertujuan untuk mengetahui efek kontaminasi saliva dan darah setelah bonding terhadap kekuatan ikat geser resin komposit dengan email dan membandingkan beberapa metode pembersihan kontaminan. 25 spesimen gigi premolar dibagi ke dalam 5 kelompok berdasarkan tipe kontaminasi dan metode pembersihan kontaminan. Kelompok kontrol menunjukkan rerata kekuatan ikat tertinggi dan rerata nilai paling rendah terdapat pada kelompok kontaminasi darah-alkohol. Terdapat perbedaan bermakna antara kelompok kontrol dengan kelompok kontaminasi saliva dan darah. Dapat disimpulkan, kontaminasi saliva dan darah setelah bonding menurunkan kekuatan ikat geser resin komposit dengan email.

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Composite resin is sensitive material that needs dry operative area. This study aimed to determine the effect of saliva and blood contamination after bonding on shear bond strength of composite resin to enamel and to compare several cleansing methods. 25 samples of human premolars were divided into 5 groups based on type of contamination and cleansing procedures. The highest bond strength was seen in control group, while group blood-alcohol showed the lowest bond strength. Significant differences were seen between control group, compared to contamination groups. In conclusion, contamination of saliva and blood after bonding decreased the shear bond strength of composite resin to enamel."

"Tingginya permintaan akan restorasi estetik, membuat pentingnya kontrol kontaminasi membutuhkan perhatian khusus karena komposit sensitif terhadap kontaminasi. Penelitian ini bertujuan untuk mengetahui efek dari kontaminasi sarung tangan, darah, dan saliva terhadap kekuatan tekan resin komposit nanohybrid. 24 spesimen dikelompokkan secara acak ke dalam empat kelompok berdasarkan tipe kontaminasi. Hasil nilai rerata dan standar deviasi (MPa) dari setiap grup adalah Grup 1= 151.57±17.97, Grup 2= 159.94±18.55, Grup 3= 134.91±25.53, Grup 4= 151.72±17.34. Secara statistik, tidak ada perbedaan secara signifikan (p>0.05) pada nilai kekuatan tekan di semua kelompok. Dapat disimpulkan bahwa kontaminasi sarung tangan, darah, dan saliva tidak mempengaruhi kekuatan tekan resin komposit nanohybrid.

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Due to highly demand of aesthetic restorations, the importance of contamination control needs a special concern since composites are sensitive to contamination. The aims of this study were to determine the effect of gloves, blood, and saliva contamination on the compressive strength of nanohybrid composite resin. 24 specimens were randomly divided into four groups based on the type of contamination.

The mean and standard deviation values (MPa) for compressive strength were Group 1= 151.57±17.97, Group 2= 159.94±18.55, Group 3= 134.91±25.53, Group 4= 151.72±17.34. All groups did not show statistically significant difference (p>0.05) in the compressive strength. In conclusion, gloves, blood, and saliva contamination do not affect the compressive strength of nanohybrid composite resin."

"ABSTRAK Odontocem® dan Biodentine®, berbahan dasar kalsium silikat. Tetapi terdapat kandungan lain yang mungkin memiliki efek pada viabiltas sel. Contohnya penambahan steroid dalam Odontocem®. Belum diketahui apakah penambahan steroid tersebut memiliki efek atau tidak terhadap viabilitas sel. Sel fibroblas yang diambil dari Telur Embrio Tertunas (TET), dipajan dengan Odontocem® dan Biodentine® dengan jumlah masing-masing kelompok sebesar 15 well. Kemudian viabilitas sel diukur selama 24 jam dan 72 jam. Hasil penelitian diuji dengan uji statistik Kruskal Wallis dengan uji post hoc Mann-Whitney. Berdasarkan hasil penelitian, didapatkan bahwa Odontocem® dan Biodentine® memiliki efek menurunkan viabiltas sel fibroblas pada pengukuran 24 jam dan 72 jam. Juga viabilitas sel fibroblas pada kelompok perlakuan Odontocem ® lebih tinggi daripada Biodentine® baik pada pengukuran 24 jam maupun 72 jam. ABSTRACT Odontocem® and Biodentine®, both consist of calcium silicate. But there are other substances which may have an effect on cell viability. For example, addition of steroids in Odontocem®. It is not yet known whether these steroids have an effect or not on the cell viability. Fibroblasts taken from Chicken Embryo, added with Odontocem® and Biodentine® by each group of 15 wells. Then the cell viability was measured for 24 hours and 72 hours. The result were tested by Kruskal Wallis statistical test with post hoc test of Mann-Whitney. Based on this research, it was found that Odontocem® and Biodentine® have a lowering effect on the fibroblast cell viability for 24 hours and 72 hours. Thus, fibroblast cell viability the treatment group Odontocem ® is higher than both the measurement Biodentine® 24 hours or 72 hours.;Odontocem® and Biodentine®, both consist of calcium silicate. But there are other substances which may have an effect on cell viability. For example, addition of steroids in Odontocem®. It is not yet known whether these steroids have an effect or not on the cell viability. Fibroblasts taken from Chicken Embryo, added with Odontocem® and Biodentine® by each group of 15 wells. Then the cell viability was measured for 24 hours and 72 hours. The result were tested by Kruskal Wallis statistical test with post hoc test of Mann-Whitney. Based on this research, it was found that Odontocem® and Biodentine® have a lowering effect on the fibroblast cell viability for 24 hours and 72 hours. Thus, fibroblast cell viability the treatment group Odontocem ® is higher than both the measurement Biodentine® 24 hours or 72 hours."

"ABSTRAK Latar Belakang: Berbagai material kaping pulpa berbahan dasar kalsium silikatterus dikembangkan, diantaranya semen berbasis kalsium silikat denganpenambahan steroid. Tujuan: Menganalisis efek penambahan steroid pada semenberbasis kalsium silikat terhadap viabilitas sel fibroblas. Metode: Sel fibroblasembrio ayam direndam dalam ekstrak larutan semen berbasis kalsium silikatdengan penambahan steroid dan MTA. Viabilitas sel dihitung dengan menggunakan uji MTT. Hasil: Terdapat perbedaan bermakna (p ≤ 0,05)viabilitas sel pada kelompok semen berbasis kalsium silikat dengan penambahansteroid dibandingkan kelompok kontrol dan MTA. Kesimpulan: Penambahansteroid menurunkan viabilitas sel. Terdapat peningkatan pada 72 jam, yang menandakan terjadinya proliferasi sel.ABSTRACT Background: Calcium silicate based materials are being developed continuously,one of them is calcium silicate based cement containing steroid. Objective: Toanalyze the effect of steroid addition in calcium silicate based cement onfibroblast cells viability. Methods: Chicken embryonic fibroblast cells wereimmersed in extract solution of calcium silicate based cement containing steroidand MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid groupwas found, compared to control and MTA group. Conclusion: Steroid additiondecrease viability. There was an increase in 72 hours, marking cells proliferation.;Background: Calcium silicate based materials are being developed continuously,one of them is calcium silicate based cement containing steroid. Objective: Toanalyze the effect of steroid addition in calcium silicate based cement onfibroblast cells viability. Methods: Chicken embryonic fibroblast cells wereimmersed in extract solution of calcium silicate based cement containing steroidand MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid groupwas found, compared to control and MTA group. Conclusion: Steroid additiondecrease viability. There was an increase in 72 hours, marking cells proliferation.;Background: Calcium silicate based materials are being developed continuously,one of them is calcium silicate based cement containing steroid. Objective: Toanalyze the effect of steroid addition in calcium silicate based cement onfibroblast cells viability. Methods: Chicken embryonic fibroblast cells wereimmersed in extract solution of calcium silicate based cement containing steroidand MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid groupwas found, compared to control and MTA group. Conclusion: Steroid additiondecrease viability. There was an increase in 72 hours, marking cells proliferation."

"ABSTRAK Latar Belakang: siler polidimetilsiloksan merupakan siler baru yang berpotensi menutup saluran akar lebih baik. Tujuan: membandingkan tingkat kerapatanpengisian di sepertiga apeks antara teknik kon tunggal (KT) dan downpackbackfill(DB) menggunakan siler polidimetilsiloksan. Metode: empat puluh gigipremolar bawah dibagi menjadi dua kelompok KT dan DB. Setelah pengisiansaluran akar, sampel diinkubasi, dilapisi cat kuku, direndam dalam tinta India, laludibuat transparan dengan metode Robertson. Kebocoran di sepertiga apeksdievaluasi dengan mikroskop stereo. Hasil: skor kebocoran terendah (0-0,5 mm)dimiliki oleh kelompok KT dan skor kebocoran tertinggi (>1 mm) dimiliki olehkelompok DB. Kesimpulan: teknik kon tunggal memberikan tingkat kerapatanyang lebih baik dibandingkan downpack-backfill, walaupun secara statistik perbedaan antar keduanya tidak signifikan. ABSTRACT Background: polydimethylsiloxane sealer is a brand new sealer that potentiallyhas a better sealing ability to the root canal. Aim: to compare the apical sealingability in one third apex between single cone (SC) and downpack-backfill (DB)technique using polydimethylsiloxane sealer. Methods: fourty extracted humanmanibular premolars devided into two group, SC and DB. After obturated withpolydimethylsiloxane sealer, samples were stored in incubator, coated with nailvarnish, immersed in India ink, and then specimens were cleared usingRobertson?s technique. The apical dye penetration was evaluated using astereomicroscope. Result: the lowest leakage score (0-0,5 mm) was found in SCgroup and the highest score(>1 mm) in DB group. Summary: single conetechnique exibited better sealing ability rather than downpack-backfill technique, although no statistical significant difference between this two techniques. ;Background: polydimethylsiloxane sealer is a brand new sealer that potentiallyhas a better sealing ability to the root canal. Aim: to compare the apical sealingability in one third apex between single cone (SC) and downpack-backfill (DB)technique using polydimethylsiloxane sealer. Methods: fourty extracted humanmanibular premolars devided into two group, SC and DB. After obturated withpolydimethylsiloxane sealer, samples were stored in incubator, coated with nailvarnish, immersed in India ink, and then specimens were cleared usingRobertson?s technique. The apical dye penetration was evaluated using astereomicroscope. Result: the lowest leakage score (0-0,5 mm) was found in SCgroup and the highest score(>1 mm) in DB group. Summary: single conetechnique exibited better sealing ability rather than downpack-backfill technique, although no statistical significant difference between this two techniques. ;Background: polydimethylsiloxane sealer is a brand new sealer that potentiallyhas a better sealing ability to the root canal. Aim: to compare the apical sealingability in one third apex between single cone (SC) and downpack-backfill (DB)technique using polydimethylsiloxane sealer. Methods: fourty extracted humanmanibular premolars devided into two group, SC and DB. After obturated withpolydimethylsiloxane sealer, samples were stored in incubator, coated with nailvarnish, immersed in India ink, and then specimens were cleared usingRobertson?s technique. The apical dye penetration was evaluated using astereomicroscope. Result: the lowest leakage score (0-0,5 mm) was found in SCgroup and the highest score(>1 mm) in DB group. Summary: single conetechnique exibited better sealing ability rather than downpack-backfill technique, although no statistical significant difference between this two techniques. "

"Latar Belakang: Semen Ionomer Kaca (SIK) adalah bahan restorasi yang terdiri dari bubuk kaca kalsium fluoroaluminosilikat dan asam oliakrilik. Pada tahap awal reaksi setelah dilakukan pencampuran, SIK sensitif terhadap udara dan air yang dapat menghambat reaksi pengerasan, sehingga perlu diberikan perlindungan dengan material yang kedap air dan salah satu material tersebut adalah bonding agent.Tujuan: Menganalisis hubungan kedalaman intrusi air terhadap kekerasan SIK.Metode: 12 spesimen SIK dengan diameter 5 mm dan tebal 2 mm, dibagi menjadi 3 kelompok: kelompok 1 tanpa bahan pelindung, kelompok 2 diaplikasikan varnis, dan kelompok 3 diaplikasikan bonding agent. Seluruh spesimen direndam dalam methylene blue 0,1% selama 24 jam dan di masukkan ke dalam inkubator dalam suhu 370C. Selanjutnya setiap sampel dibelah menjadi 2 bagian, pada satu bagian dilakukan pengukuran terhadap kedalaman intrusi air menggunakan measuring microscope sedangkan pada satu sisi lainnya dilakukan pengukuran terhadap kekerasan menggunakan Knoop Microhardness Tester. Kemudian hasilnya dianalisis secara statistik.Hasil: Pada tiap kelompok terdapat hubungan korelasi yang kuat antara kedalaman intrusi air dan kekerasan SIK dengan nilai korelasi -0,868 dan nilai p < 0,05 .Kesimpulan: Semakin dalam intrusi air pada SIK, semakin rendah kekerasan SIK.

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Background: Glass Ionomer Cement (GIC) is a restorative material consisting of calcium fluoroaluminosillicate glass powder and polyacrylic acid. At the initial reaction after mixing, GIC is sensitive to the air and water which can inhibit the setting reaction, therefore it is needed a protection by materials which are watertight and one of them is bonding agent.Aim: To analyze the relation of the depth water intrusion to the hardness of GIC.Method: 12 specimen with 5 mm in diameter and 2 mm in thickness, were divided into 3 groups: the first group wasn?t given a coating, the second group was given varnish, and the third group was given bonding agent. All specimen were soaked in methylene blue 0,1% during 24 hours and placed in incubator with 370C in temperature. Furthermore, each sample was cut into 2 parts, one part was measured to know the depth of water intrusion by measuring microscope while the other part was measured to know its hardness by Knoop Microhardness Tester. Afterwards, the result is analyzed statistically.Result: There is a strong correlation between the depth of water intrusion and the hardness of GIC in each group with correlation value -0,868 and p value < 0,05.Conclusion: The deeper the water intrusion of GIC, the lower the hardness of GIC."

"Latar Belakang: Semen Ionomer Kaca (SIK) adalah bahan restorasi yang terdiri dari bubuk kaca kalsium fluoroaluminosilikat dan asam poliakrilik. Pada tahap awal reaksi setelah pencampuran, SIK sensitif terhadap udara dan air yang dapat menghambat reaksi pengerasan sehingga perlu perlindungan dengan material yang kedap air dan salah satunya adalah bonding agent.Tujuan: menganalisis efek bonding agent terhadap kedalaman intrusi air pada SIK.Metode: 12 spesimen SIK diameter 5 mm dan tebal 2 mm, dibagi menjadi 3 kelompok: kelompok 1 tanpa pelapisan, kelompok 2 dilapis varnis, dan kelompok 3 dilapis bonding agent. Seluruh spesimen direndam dalam methylene blue 0,1% selama 24 jam dan dimasukkan dalam inkubator dengan suhu 370 C. Kemudian setiap sampel dibelah menjadi 2, yang satu sisi diukur kedalaman intrusi airnya menggunakan measuring microscope dan bagian lainnya diukur kekerasan permukaannya menggunakan Knoop Microhardness Tester. Kemudian hasilnya dianalisis secara statistik.Hasil: Pada ketiga kelompok terlihat adanya perbedaan bermakna dengan nilai kedalaman intrusi air tertinggi ada pada kelompok tanpa perlakuan dan paling rendah pada kelompok bonding agent.Kesimpulan: Aplikasi bonding agent dapat menurunkan kedalaman intrusi air pada SIK.

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Background: Glass Ionomer Cement (GIC) is a restorative material containing calcium fluoroaluminosilicate glass powder and polyacrylic acid. At initiation stage of reaction after mixing, GIC becomes sensitive with air and water which can inhibit setting reaction so it needs to be protected with waterproof material, the one is bonding agent.Aim: To analize bonding agent?s effect in depth of water intrusion in GIC.Method: 12 GIC speciments with 5 mm in diameter and 2 mm in height were divided into 3 groups: group 1 without any protecting layer, group 2 was coated with varnish, and group 3 was coated with bonding agent. All speciments were immersed in methylene blue 0,1% as long as 24 hours and was put into incubator 370 C. Then, each samples was cut off into 2 pieces, one side was measured for water intrusion using measuring microscope dan the other was measured for surface hardness using Knoop Microhardness Tester. After that, the result was analized statistical.Result: At 3 groups showed there was significant difference, the highest water intrusion depth score was group without any protecting layer and the lowest score was bonding agent?s group.Conclusion: Application of bonding agent could decrease the depth of water intrusion in GIC."