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"ABSTRAKBerbagai penelitian dalam bidang kedokteran gigi memerlukan sajian histologi yang baik dan terbaca dibawah mikroskop. Gigi dan jaringan pendukungnya terdiri dari jaringan keras dan jaringan lunak yang sama pentingnya, karena itu diperlukan tindakan dekalsifikasi yang dapat melunakan jaringan keras (dentin, sementum, tulang) agar dapat diiris dengan mikrotom, dan memberi hasil memuaskan pada jaringan lunak (gingiva, pulpa, ligamen periodontal). Pada penelitian yang dilakukan pada rahang tikus LMR ini, dibandingkan tiga bahan dekalsifikasi yaitu larutan sovin, EDT A 5% pH 7 dan asam formiat 5%, sebagian diwarna hematoksilin Eosin dan sebagian lagi Trikrom Masson Goldner, serta pengaruhnya terhadap hasil akhir sajian histology. Sajian dinilai oleh 3 pemeriksa secara uji buta. Hasil penelitian ditabulasi dan dianalisis dengan Kruskal Wallis one way for analysis of variances. Pada sajian yang diwarna HE, analisis statistik menunjukkan hasil berbeda bermakna. Kombinasi Bovin-HE memberi hasil paling melunakan mendekati sangat jelas (3,4), kemudian EDTA-HE (3,2) dan terakhir Formiat-HE (dengan nilai 3 = jelas. Dengan pewarna Trikrom Masson Goldner, analisis statistik tidak menunjukkan perbedaan bermakna dengan nilai rata-rata 2,5 (antara jelas dan kurang jelas). "

"Aim: to evaluate the role of clinical characteristics, functional markers of vasodilation, inflammatory response, and atherosclerosis in predicting wound healing in diabetic foot ulcer.

Methods: a cohort study (February-October 2010) was conducted from 40 subjects with acute diabetic foot ulcer at clinical ward of Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, Indonesia. Each subject underwent at least two variable measurements, i.e. during inflammatory phase and proliferation phase. The studied variables were clinical characteristics, complete peripheral blood count (CBC) and differential count, levels of HbA1c, ureum, creatinine, lipid profile, fasting blood glucose (FBG), marker of endothelial dysfunction (asymmetric dimethylarginine/ADMA, endothelin-1/ET-1, and flow-mediated dilation/FMD of brachial artery), and marker of vascular calcification (osteoprotegerin/OPG).

Results: median of time achieving 50% granulation tissue in our study was 21 days. There were nine factors that contribute in the development of 50% granulation tissue, i.e. family history of diabetes mellitus (DM), previous history of wound, wound area, duration of existing wound, captopril and simvastatin medications, levels of ADMA, ET-1, and OPG. There were three out of the nine factors that significantly correlated with wound healing, i.e. wound area, OPG levels, and simvastatin medications.

Conclusion: in acute diabetic foot ulcers, wound area and OPG levels had positive correlation with wound healing, whereas simvastatin medications had negative correlation with wound healing."