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ABSTRAKLATAR BELAKANG: Laki-laki dengan penuaan pada umumnya mengalami
penurunan kualitas hidup dan infertilitas, penyebabnya berkaitan dengan degenerasi
sel Leydig akibat akumulasi stres oksidatif sehingga terjadi penurunan kadar
testosteron. Daun Enhalus acoroides dilaporkan mengandung antioksidan yang dapat
menangkal radikal bebas dan fitosterol yang dapat diubah menjadi testosteron.
Penelitian ini dilakukan untuk mengetahui efek ekstrak etil asetat daun lamun
[Enhalus acoroides (L.f.) Royle] terhadap analisis semen, morfologi sel Leydig, dan
stres oksidatif mencit jantan tua
BAHAN DAN CARA KERJA: Sampel berjumlah 32 ekor mencit jantan galur DDY
yang diberikan ekstrak daun Enhalus acoroides selama 14 hari. Mencit dibagi dalam
8 kelompok perlakuan masing masing terdiri dari 4 kali ulangan yang terdiri dari
DK1 = dewasa kontrol 1 (mencit dewasa tanpa perlakuan); DK2=dewasa kontrol 2
(mencit dewasa minyak zaitun); DP1=dewasa perlakuan 1 (mencit dewasa ekstrak 25
mg/KgBB); DP2=dewasa perlakuan 2 (mencit dewasa ekstrak 50 mg/KgBB);
TK1=tua kontrol 1 (mencit tua tanpa perlakuan); TK2=tua kontrol 2 (mencit tua
minyak zaitun); TP1=tua perlakuan 1 (mencit tua ekstrak 25 mg/KgBB); TP2=tua
perlakuan 2 (mencit tua ekstrak 50 mg/KgBB).
HASIL: Peningkatan motilitas dan viabilitas spermatozoa mencit tua pada dosis 50
mg/KgBB, peningkatan morfologi spermatozoa normal pada mencit tua dengan dosis
25mg/KgBB dan penurunan konsentrasi spermatozoa mencit tua pada dosis 25
mg/KgBB. Adanya peningkatan sel Leydig tua pada dosis 25 mg/KgBB
KESIMPULAN: Pemberian ekstrak daun Enhalus acoroides meningkatkan kualitas
spermatozoa. Namun tidak ada perbedaan dalam penundaan degenerasi sel Leydig
dewasa serta tidak ada perbedaan pada tingkat stres oksidatif
ABSTRACTBackground: The men aging process occurs generally due to decreased quality of
life and infertility, the cause associated with Leydig cell degeneration due to
accumulation of oxidative stress resulting in a decrease in testosterone levels. At this
time the administration of synthetic antioxidants is limited because of the risk of
cancer, on the other hand administration of synthetic testosterone cause the cessation
of endogenous testosterone production and long-term risk. Lamun leaves Enhalus
acoroides reported to contain antioxidants which can counteract free radicals and
phytosterols that can be converted into testosterone. Therefore, this study was
conducted to determine effect of ethyl acetate extract of lamun leaves Enhalus
acoroides on semen analysis, Leydig cell morphology, and oxidative stress in old
male mice
Methods: The sample amounted to 32 mice (mus Musculus) strain DDY male given
extract of the leaves of seagrass for 14 days. Mice were divided into eight treatment
groups each consisting of four replications. Four groups are composed of adult mice
which consist of DK1=adult control 1, DK2= adult control 2 (adult mice with olive
oil), DP1= adult treatment 1 (extract 25 mg/KgBW), DP2=adult treatment 2 (extract
50 mg/KgBW). 4 groups male older mice consist of TK1 = old control 1, TK2= old
control 2 (old mice with olive oil), TP1= old treatment 1 (extract 25 mg/KgBW),
TP2= old treatment 2 (extract 50 mg/KgBW).
Results: An increased motility and viability of sperm at doses of 50 mg/KgBW,
increased morphology of sperm normally at doses of 25 mg/KgBW, and decreased
the concentration of sperm at doses of 25 mg/KgBW. An increased in aged Leydig
cells at doses 25 mg/KgBW
Conclusion: The extract of lamun leaves can qualitatively improve sperm, however
not significant in delays degeneration of adult Leydig cells and not significant
decreased levels of oxidative stress in old male mice"
