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"Latar belakang: Studi ini dilakukan untuk mempelajari pengaruh pemberian ekstrak akar Acalypha indica Linn terhadap viabilitas relatif dan proliferasi sel sebagai parameter neurogenesis pada kultur jaringan hipokampus tikus pascahipoksia.Metode: Studi eksperimental in vitro pada 24 kultur primer jaringan sel saraf tikus Sprague Dowley dewasa yang dipajankan terhadap hipoksia dengan gas 5% O2/5% CO2/N2 seimbang selama 24 jam. Pascahipoksia, ekstrak Acalypha indica Linn ditambahkan pada 3 kelompok perlakuan, masing-masing dengan dosis 10, 15, dan 20 mg/mL, sedangkan pada kelompok kontrol tidak ditambahkan apapun. Setiap kelompok terdiri atas 6 sampel. Setelah inkubasi selama 90 jam, viabilitas relatif sel diukur dengan 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), proliferasi sel diukur dengan 5-bromo2â??-deoxy-uridine (BrdU). Data dianalisis dengan menggunakan tes parametrik one way ANOVA yang dilanjutkan dengan analisis post-hoc.Hasil: Viabilitas relatif sel pada kultur jaringan hipokampus tikus pascahipoksia dengan pemberian ekstrak akar kucing pada dosis 10, 15, dan 20 mg/mL lebih tinggi secara bermakna dibandingkan dengan kontrol (176,95%, 220,62%, 386,02% vs. 100%). Proliferasi sel pada kultur jaringan hipokampus tikus pascahipoksia dengan pemberian ekstrak akar kucing pada dosis 10, 15, dan 20 mg/mL lebih tinggi secara bermakna dibandingkan dengan kontrol (0,132; 0,117; 0,114 vs. 0,096).Kesimpulan: Ekstrak Acalypha indica Linn dapat meningkatkan viabilitas relatif dan proliferasi sel pascahipoksia in vitro pada dosis 10, 15, dan 20 mg/mL. (Med J Indones 2011; 20:94-9)

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AbstractBackground: This research was done to study the infl uence of Acalypha indica Linn root extract towards relative cell viability and proliferation as parameters of neurogenesis in post-hypoxic hippocampal tissue culture.Methods Experimental in vitro study using 24 primary neuronal cell cultures obtained from adult Sprague Dawley rat exposed to hypoxia with 5% O2/5% CO2/N2 balance gas for 24 hours. Post-hypoxia, Acalypha indica Linn root extract was added at doses of 10, 15, and 20 mg/mL to 3 treatment groups. No treatment was given to the control group. Each group consists of 6 samples. After 90 hours of incubation, relative cell viability was measured by using 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) examination, and cell proliferation was measured by using 5-bromo2â??-deoxy-uridine (BrdU) for cell proliferation. Data was analyzed using one way ANOVA parametric tests, then further analyzed with post-hoc analysis.Results: The relative cell viability of rat hippocampal tissue culture treated with Acalypha indica Linn root extract with dose of 10, 15, and 20 mg/mL was signifi cantly higher than control (176.95%, 220.62%, and 386.02% vs. 100%). Cell proliferation of rat hippocampal tissue culture treated with Acalypha indica Linn root extract with dose of 10, 15, and 20 mg/mL was signifi cantly higher than control (0.132, 0.117, 0.114 vs 0.096).Conclusion: Acalypha indica Linn root extract with doses of 10, 15, and 20 mg/mL can increase relative cell viability and proliferation in post-hypoxic hippocampal tissue culture. (Med J Indones 2011; 20:94-9)"

"Saliva and Streptococcus mutans play role in biofilm formation. Saliva and S.mutans virulence are different between subjects with and without caries. Objective: The aim of this study was to evaluate the effects of autolog saliva on biofilm formation of S. mutans isolated from caries and caries-free subjects. Materials and Methods: Saliva and plaque samples are obtained from caries and caries-free subjects. Plaque samples were cultured on TYS20B for 3 days. Selected colonies were picked and cultured on TSB for 3 days. After colony counting, biofilm assay was conducted and inoculated for one day. The biofilm was tested using crystal violet binding assay and quantified by measuring the optical density at 655 nm wavelength. Result: The optical density of S. muttans biofilm isolated from subjects with caries were different from taste with no caries. Biofilm formation of S. muttans isolated from caries and caries-free subjects with and without the presence of autolog saliva were different. Conclusion: Autolog saliva influences S. mutans biofilm formation and there is a tendency that is higher than those from subjects with no caries. "