Hasil Pencarian

Hasil Pencarian  ::  Simpan CSV :: Kembali

Hasil Pencarian

Ditemukan 50 dokumen yang sesuai dengan query
cover
Siahaan, Samuel Dominggus Chandra
"Metode In Vitro Fertilization (IVF) sudah dikenal sebagai salah satu metode reproduksi berbantu yang efektif menangani masalah infertilitas. Angka kehamilan IVF siklus baru Indonesia tahun 2013 untuk usia <35 tahun berada di angka 41,48%, masih di bawah angka kehamilan siklus IVF pada usia sama di dunia tahun 2012, yaitu 46,6%. Terdapat beberapa faktor yang dapat berpengaruh pada angka kehamilan siklus IVF, salah satunya adalah fase embrio saat transfer embrio. Berdasarkan penelitian sebelumnya ditemukan bahwa fase blastokista memiliki angka kehamilan yang lebih tinggi. Tujuan penelitian ini adalah untuk meningkatkan efektivitas prosedur IVF melalui pemahaman akan faktor yang mempengaruhinya. Penelitian dilakukan dengan menggunakan data dari rekam medik Klinik Yasmin RSCM Kencana. Sampel penelitian dibagi ke dalam dua kelompok, 120 pasien grup fase pembelahan dan 120 pasien grup fase blastokista. Pemilihan sampel menggunakan metode systematic random sampling. Pada analisis didapatkan beda proporsi antara grup fase blastokista dengan grup fase pembelahan sebesar 20,8% (50,8% dan 30,0%; p= 0,002). Pada analisis multivariat didapatkan tiga variabel perancu yang memiliki pengaruh dalam penelitian, yaitu tebal endometrium, endometriosis, dan faktor tuba. Disimpulkan bahwa transfer embrio pada fase blastokista memiliki angka kehamilan lebih tinggi bermakna dibandingkan dengan fase pembelahan;In Vitro Fertilization (IVF) is known as one of assisted reproduction methods that effectively addresses the problem of infertility. IVF fresh cycle pregnancy rate in Indonesia for age <35 years in 2013 was at 41.48%. It was still below the pregnancy rate of fresh IVF cycles at the same age worldwide in 2012, 46.6%. There are several factors that can affect the pregnancy rate of IVF cycles, one of which is the stage when the embryo is transferred. Based on previous studies, it was found that the blastocyst stage have a higher pregnancy rate than the cleavage stage. The purpose of this study was to improve IVF effectiveness. The study was conducted using medical records from Klinik Yasmin RSCM Kencana. Samples were divided into two groups, 120 patients in cleavage stage group and 120 patients in blastocyst stage group. The samples were selected using systematic random sampling method. In the analysis, we found 20,8% proportion difference between the blastocyst stage group to the cleavage stage group (50.8% and 30.0%; p = 0.006). In the multivariate analysis, it was found that there were three confounding variables, endometrial thickness, endometriosis, and tubal factor, which significantly affected pregnancy rate. It was concluded that blastocyst embryo transfer have a significantly higher pregnancy rate than cleavage embryo transfer."
Lengkap +
2015
S-Pdf
UI - Skripsi Membership  Universitas Indonesia Library
cover
Markus Christian Hartanto
"Frozen Embryo Transfer (FET) merupakan metode transfer embrio yang memiliki keunggulan dibandingkan dengan fresh embryo transfer (ET) karena tidak melalui proses stimulasi ovulasi. Berbagai penelitian sudah dilakukan untuk membandingkan tingkat keberhasilan FET dan ET, namun hasil yang didapat masing-masing penelitian menunjukkan angka yang berbeda, serta ada yang signifikan dan tidak. Penelitian ini bertujuan untuk mengetahui tingkat keberhasilan kehamilan pasien yang menjalani FET dan dibandingkan dengan yang menjalani ET. Penelitian dilakukan dengan metode kohort retrospektif, dengan menggunaakan 288 data rekam medis Klinik Yasmin Departemen Obstetri dan Ginekologi Rumah Sakit Cipto Mangunkusumo. Kehamilan ditentukan dengan kadar hCG ≥50 mIU/mL. Data kemudian dianalisis menggunakan program SPSS 21.0 for Windows dengan uji chi-square untuk melihat hubungan jenis transfer embrio dengan tingkat keberhasilan kehamilan. Selain itu, faktorfaktor lain yang mempengaruhi tingkat keberhasilan kehamilan juga dianalisis secara multivariat dengan metode regresi logistik. Hasil penelitian menunjukkan angka kehamilan FET (39,6%) lebih tinggi daripada angka kehamilan ET (38,2%) namun tidak menunjukkan hubungan yang signifikan (p=0,809). Faktor-faktor yang berhubungan secara signifikan dengan keberhasilan kehamilan adalah adanya masalah ovarium, penurunan cadangan ovarium, dan masalah infertilitas pada laki-laki.

Frozen Embryo Transfer (FET) is an embryo transfer method on in vitro fertilization that has advantage compared to fresh embryo transfer (ET) because it does not need ovulatory stimulation. Several studies have been done to compare pregnancy rate outcome of FET and ET, but the results show different number and significancy. The aim of this research was to know the pregnancy rate of FET patients compared to ET. The research was retrospective cohort study and used 288 medical record datas in Obstetry and Gynecology Department of Cipto Mangunkusumo National Hospital. Pregnancy was measured by hcG level ≥50 mIU/mL on day 15. All results were statistically analysed by SPSS 21.0 for windows by using chi-square test to know the relation of embryo transfer method and pregnancy rate. Besides that, the other factors that affected pregnancy rate was also analyzed with multivariate logistic regression method. The result showed FET pregnancy rate (39,6%) was higher than ET (38,2%) but not statistically significant (p=0,809). Factors that had significant correlation with pregnancy rate was ovary problem, decrease of ovarian reserve, and male infertility problems"
Lengkap +
2015
S-Pdf
UI - Skripsi Membership  Universitas Indonesia Library
cover
Indra
"ABSTRAK
Latar Belakang: Sindroma ovarium polikistik (SOPK) merupakan kelainan
kompleks endokrin terbanyak pada usia reproduksi. Ketidakseimbangan hormon
merupakan salah satu temuan SOPK yang terkait dengan patofisiologinya.
Adanya perbedaan kejadian resistensi insulin mungkin terkait dengan perbedaan
karakteristik hormon pada pasien SOPK pada berbagai fenotipe, salah satunya
Hormon Anti-Mullerian (HAM).
Metode: Desain penelitian potong lintang (cross sectional), pengambilan subjek
dimulai dari identifikasi kelompok dengan SOPK menggunakan titik potong
HAM >4,45 ng/dl, lalu akan dikelompokkan menjadi empat kelompok sesuai
dengan fenotipnya berdasarkan kriteria Rotterdam 2003, di klinik Yasmin dan
Makmal Endokrinologi di bagian Obstetri dan Ginekologi RSUPN Dr.Cipto
Mangunkusumo selama tahun 2011 ?2014. Resistensi insulin dinilai
menggunakan homeostatic model assessment for insulin resistance (HOMA IR).
Hasil: Didapatkan 125 subjek SOPK dengan kadar HAM cenderung lebih tinggi
pada fenotipe 1 dan lebih rendah pada fenotipe 4 (p<0,05). Selain itu, ditemukan
perbedaan kadar LH dan rasio LH/FSH pada keempat fenotipe (p<0,05). Nilai
HOMA IR juga lebih tinggi pada fenotipe 1 (p<0,05). Berdasarkan analisis regresi
linear, didapatkan indeks massa tubuh dan HAM berkorelasi positif dengan nilai
HOMA IR (r=0,19 p=0,015 dan r=0,53 p<0,001, berturut-turut).
Kesimpulan: Terdapat korelasi positif kadar HAM terhadap nilai HOMA IR.
Pada keempat fenotipe SOPK didapatkan kadar HAM dan HOMA IR lebih tinggi
pada fenotipe 1 dan semakin rendah pada fenotipe 4. ABSTRACT Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder that is
the most complex in the reproductive age. Hormonal imbalance remains a part of
its pathophysiology. Insulin resistance might be linked with difference in
hormonal parameter across four phenotypes of PCOS, especillay in AMH level.
Method: The study design was cross-sectional (cross-sectional). Subject retrieval
starts from the identification of the group with PCOS (with cutt-off AMH serum
>4.45 ng/dl), and will be grouped into four groups according to their phenotype
based on the criteria of Rotterdam in 2003, in the clinic Yasmin and
Endocrinology Laboratory at the Obstetrics and Gynecology RSUPN Dr.Cipto
Mangunkusomo during 2011 -2014. Insulin resistance was counted using
homeostatic model assessment for insulin resistance (HOMA IR).
Result: A total of 125 PCOS patients were recruited with AMH serum
significantly higher among phenotype 1 and lower among phenotype 4 (p<0.05).
Besides, Lh serum and LH/FSH ratio were also different across four phenotypes
(p<0.05). The value of HOMA IR tend to be higher among phenotype 1 (p<0.05).
Using multivariable linear regression analysis, body mass index and AMH serum
were positively correlated with HOMA IR (r=0.19 p=0.015 and r=0.53 p<0.001,
respectively).
Conclusion: There was a positive correlation between AMH serum and HOMA
IR value. The four PCOS phenotypes had significantly different value of HAM
serum and HOMA IR with phenotype 1 possed higher value among the others.
;Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder that is
the most complex in the reproductive age. Hormonal imbalance remains a part of
its pathophysiology. Insulin resistance might be linked with difference in
hormonal parameter across four phenotypes of PCOS, especillay in AMH level.
Method: The study design was cross-sectional (cross-sectional). Subject retrieval
starts from the identification of the group with PCOS (with cutt-off AMH serum
>4.45 ng/dl), and will be grouped into four groups according to their phenotype
based on the criteria of Rotterdam in 2003, in the clinic Yasmin and
Endocrinology Laboratory at the Obstetrics and Gynecology RSUPN Dr.Cipto
Mangunkusomo during 2011 -2014. Insulin resistance was counted using
homeostatic model assessment for insulin resistance (HOMA IR).
Result: A total of 125 PCOS patients were recruited with AMH serum
significantly higher among phenotype 1 and lower among phenotype 4 (p<0.05).
Besides, Lh serum and LH/FSH ratio were also different across four phenotypes
(p<0.05). The value of HOMA IR tend to be higher among phenotype 1 (p<0.05).
Using multivariable linear regression analysis, body mass index and AMH serum
were positively correlated with HOMA IR (r=0.19 p=0.015 and r=0.53 p<0.001,
respectively).
Conclusion: There was a positive correlation between AMH serum and HOMA
IR value. The four PCOS phenotypes had significantly different value of HAM
serum and HOMA IR with phenotype 1 possed higher value among the others.
;Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder that is
the most complex in the reproductive age. Hormonal imbalance remains a part of
its pathophysiology. Insulin resistance might be linked with difference in
hormonal parameter across four phenotypes of PCOS, especillay in AMH level.
Method: The study design was cross-sectional (cross-sectional). Subject retrieval
starts from the identification of the group with PCOS (with cutt-off AMH serum
>4.45 ng/dl), and will be grouped into four groups according to their phenotype
based on the criteria of Rotterdam in 2003, in the clinic Yasmin and
Endocrinology Laboratory at the Obstetrics and Gynecology RSUPN Dr.Cipto
Mangunkusomo during 2011 -2014. Insulin resistance was counted using
homeostatic model assessment for insulin resistance (HOMA IR).
Result: A total of 125 PCOS patients were recruited with AMH serum
significantly higher among phenotype 1 and lower among phenotype 4 (p<0.05).
Besides, Lh serum and LH/FSH ratio were also different across four phenotypes
(p<0.05). The value of HOMA IR tend to be higher among phenotype 1 (p<0.05).
Using multivariable linear regression analysis, body mass index and AMH serum
were positively correlated with HOMA IR (r=0.19 p=0.015 and r=0.53 p<0.001,
respectively).
Conclusion: There was a positive correlation between AMH serum and HOMA
IR value. The four PCOS phenotypes had significantly different value of HAM
serum and HOMA IR with phenotype 1 possed higher value among the others.
"
Lengkap +
Fakultas Kedokteran Universitas Indonesia, 2015
SP-PDF
UI - Tugas Akhir  Universitas Indonesia Library
cover
Nabil Hasnaa Khoirunnisa
"

Pendahuluan: Kelainan kromosom meningkat secara eksponensial pada ibu usia lanjut dan prevalensi dapat mencapai hingga 80% pada wanita usia 40 tahun. Oleh karena itu, PGT-A telah digunakan di rumah sakit pada pasien IVF, untuk mencegah konsekuensi yang timbul dari kelainan genetik dan mengoptimalkan hasil klinis. Faktor ibu dan faktor laki-laki yang berhubungan dengan kesuburan telah dikaitkan dengan penemuan embrio aneuploid di PGT-A, namun data masih sedikit. Tujuan penelitian ini adalah menganalisis pengaruh usia ibu, penilaian embrio, konsentrasi sperma, dan motilitas sperma pasangan terhadap hasil PGT-A. Metode: Penelitian cross-sectional ini menggunakan data rekam medis dan buku embriologi Klinik Yasmin Kencana. Sebanyak 35 pasien IVF dengan embrio tunggal yang telah menjalani PGT-A dianalisis menggunakan uji Kruskal Wallis untuk kemungkinan pengaruh usia ibu, penilaian embrio, konsentrasi sperma, dan motilitas sperma pasangan, terhadap hasil PGT-A. Untuk mengidentifikasi faktor terkuat yang terkait dengan tingkat aneuploidi, dilakukan analisis regresi logistik multivariat. Hasil: Usia ibu (p=0,033), penilaian embrio (p=0,002), konsentrasi sperma (p=0,002) dan motilitas sperma (p=0,008), memiliki hubungan yang signifikan secara statistik dengan hasil PGT-A. Faktor terkuat dalam memprediksi hasil PGT-A pasien IVF adalah penilaian embrio (B=0.593, OR=1.809, 95% CI, 0.286-0.683, p<0.001). Kesimpulan: Penelitian ini menemukan bahwa usia ibu, penilaian embrio, konsentrasi sperma dan motilitas sperma mempengaruhi hasil PGT-A.


Introduction: Chromosomal abnormality increases exponentially in advanced maternal age and reaches up to 80% prevalence in women ages 40. For this reason, PGT-A has been utilized in hospital settings for IVF patients, to prevent consequences arising from genetic anomalies and optimize clinical outcomes. Maternal factors and fertility-related male factors have been associated with finding an aneuploid embryo in PGT-A, yet data remain scant. The aim of this study was to analyze the influence of maternal age, embryo grading, partner’s sperm concentration and sperm motility on PGT-A results. Method: This cross-sectional study used data from Yasmin Kencana Clinic medical record and embryology book. A total of 35 single-embryo IVF patients who had undergone PGT-A were analyzed using the Kruskal Wallis test, for the possible influence of maternal age, embryo grading, partner’s sperm concentration and sperm motility on PGT-A results. For the identification of the strongest factor associated with aneuploidy rates, multivariate logistic regression analysis was performed. Results: Maternal age (p=0.033), embryo grading (p=0.002), sperm concentration (p=0.002) and sperm motility (p=0.008), have a statistically significant relationship with the PGT-A results. The strongest factor in predicting PGT-A results of IVF patients is embryo grading (B=0.593, OR=1.809, 95% CI, 0.286- 0.683, p<0.001). Conclusion: This study found that maternal age, embryo grading, sperm concentration and sperm motility influence PGT-A results.

"
Lengkap +
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2023
S-pdf
UI - Skripsi Membership  Universitas Indonesia Library
cover
Wilton Wylie Iskandar
"[ABSTRAK
Azoospermia adalah kondisi tidak adanya sperma dalam ejakulat pria. Azoospermia ditemukan pada 1% pria dan 10-15% pada kasus infertilitas pria. Saat ini, Intracytoplasmic Sperm Injection (ICSI) diketahui sebagai tata laksana infertilitas pria dengan azoospermia. Keberhasilan kehamilan teknik ICSI tidak hanya dipengaruhi oleh tipe pengambilan sperma, tetapi juga oleh faktor perempuan seperti usia perempuan, ketebalan endometrium, dan adanya indikasi teknik bayi tabung pada perempuan. Saat ini belum ada studi yang menunjukkan keberhasilan kehamilan pada pasien azoospermia yang menggunakan teknik ICSI. Tujuan penelitian untuk mengetahui faktor-faktor yang mempengaruhi keberhasilan kehamilan bayi tabung pada pasien azoospermia yang menggunakan teknik ICSI. Penelitian ini dilakukan menggunakan data sekunder yang diperoleh dari Klinik Yasmin Departemen Obstetri dan Ginekologi Fakultas Kedokteran Universitas Indonesia pada Januari 2010-Oktober 2015. Data dianalisis menggunakan SPSS versi 16 secara bivariat menggunakan uji khi kuadrat untuk data kategorik dengan nilai p <0.05 dianggap signifikan. Selanjutnya dilakukan analisis multivariat menggunakan uji regresi logistik untuk menilai hubungan antar variabel dengan keberhasilan kehamilan. Pada uji bivariat, variabel usia perempuan memiliki hubungan bermakna dengan RO 3.382 dan IK95% berkisar antara 1.205 - 9.494. Kesimpulan: Teknik pengambilan sperma, ketebalan endometrium, dan adanya indikasi teknik bayi tabung tidak berhubungan dengan keberhasilan kehamilan pada pasien azoospermia yang menjalankan teknik ICSI, melainkan hanya usia wanita.

ABSTRAK
Azoospermia was defined as the absence of spermatozoa in ejaculate. Azoospermia was found in 1% men and 10-15% cases of male infertility in the world. Intracytoplasmic Sperm Injection (ICSI) was known as a management of male infertility. The success rate of ICSI did not only influenced by types of sperm retrieval, but also by female factors such as female age, endometrium thickness, and the presence of female’s in vitro fertilization indication. However, there was no study that shows success rate of ICSI on azoospermic patient. Objective of this study is to know factors that determine the success rate of ICSI on azoospermic patient. This study was conducted using secondary data from Klinik Yasmin Departemen Obstetri dan Ginekologi Fakultas Kedokteran Universitas Indonesia form January 2010- October 2015. These categorical data was analyzed using SPSS version 16, using chi square and p value < 0.05 was considered statistically significant. Multivariate logistic regression was done to test correlation between variables and success rate. The women’s age significantly influence the success rate of ICSI on azoospermic patient with RO 3.382 and CI95% range within 1.205 – 9.494. To conclude, type of sperm retrieval, endometrial thickness, and the presence of in vitro fertilization indication in women did not influence success rate of ICSI on azoospermic patient. Only women’s age that determined the success rate of ICSI on azoospermic patient.
;Azoospermia was defined as the absence of spermatozoa in ejaculate. Azoospermia was found in 1% men and 10-15% cases of male infertility in the world. Intracytoplasmic Sperm Injection (ICSI) was known as a management of male infertility. The success rate of ICSI did not only influenced by types of sperm retrieval, but also by female factors such as female age, endometrium thickness, and the presence of female’s in vitro fertilization indication. However, there was no study that shows success rate of ICSI on azoospermic patient. Objective of this study is to know factors that determine the success rate of ICSI on azoospermic patient. This study was conducted using secondary data from Klinik Yasmin Departemen Obstetri dan Ginekologi Fakultas Kedokteran Universitas Indonesia form January 2010- October 2015. These categorical data was analyzed using SPSS version 16, using chi square and p value < 0.05 was considered statistically significant. Multivariate logistic regression was done to test correlation between variables and success rate. The women’s age significantly influence the success rate of ICSI on azoospermic patient with RO 3.382 and CI95% range within 1.205 – 9.494. To conclude, type of sperm retrieval, endometrial thickness, and the presence of in vitro fertilization indication in women did not influence success rate of ICSI on azoospermic patient. Only women’s age that determined the success rate of ICSI on azoospermic patient.
;Azoospermia was defined as the absence of spermatozoa in ejaculate. Azoospermia was found in 1% men and 10-15% cases of male infertility in the world. Intracytoplasmic Sperm Injection (ICSI) was known as a management of male infertility. The success rate of ICSI did not only influenced by types of sperm retrieval, but also by female factors such as female age, endometrium thickness, and the presence of female’s in vitro fertilization indication. However, there was no study that shows success rate of ICSI on azoospermic patient. Objective of this study is to know factors that determine the success rate of ICSI on azoospermic patient. This study was conducted using secondary data from Klinik Yasmin Departemen Obstetri dan Ginekologi Fakultas Kedokteran Universitas Indonesia form January 2010- October 2015. These categorical data was analyzed using SPSS version 16, using chi square and p value < 0.05 was considered statistically significant. Multivariate logistic regression was done to test correlation between variables and success rate. The women’s age significantly influence the success rate of ICSI on azoospermic patient with RO 3.382 and CI95% range within 1.205 – 9.494. To conclude, type of sperm retrieval, endometrial thickness, and the presence of in vitro fertilization indication in women did not influence success rate of ICSI on azoospermic patient. Only women’s age that determined the success rate of ICSI on azoospermic patient.
, Azoospermia was defined as the absence of spermatozoa in ejaculate. Azoospermia was found in 1% men and 10-15% cases of male infertility in the world. Intracytoplasmic Sperm Injection (ICSI) was known as a management of male infertility. The success rate of ICSI did not only influenced by types of sperm retrieval, but also by female factors such as female age, endometrium thickness, and the presence of female’s in vitro fertilization indication. However, there was no study that shows success rate of ICSI on azoospermic patient. Objective of this study is to know factors that determine the success rate of ICSI on azoospermic patient. This study was conducted using secondary data from Klinik Yasmin Departemen Obstetri dan Ginekologi Fakultas Kedokteran Universitas Indonesia form January 2010- October 2015. These categorical data was analyzed using SPSS version 16, using chi square and p value < 0.05 was considered statistically significant. Multivariate logistic regression was done to test correlation between variables and success rate. The women’s age significantly influence the success rate of ICSI on azoospermic patient with RO 3.382 and CI95% range within 1.205 – 9.494. To conclude, type of sperm retrieval, endometrial thickness, and the presence of in vitro fertilization indication in women did not influence success rate of ICSI on azoospermic patient. Only women’s age that determined the success rate of ICSI on azoospermic patient.
]"
Lengkap +
2016
S-Pdf
UI - Skripsi Membership  Universitas Indonesia Library
cover
Kinandra Rafa Khalisha Rambey
"Latar Belakang: Growth Differentiation Factor 9 (GDF9) adalah salah satu anggota dari superfamili TGF-β yang merupakan salah satu oocyte-secreted factor (OSF). GDF9 memainkan peran penting dalam folikulogenesis ovarium, kompetensi perkembangan oosit, serta sebagai molekul esensial yang mengontrol berbagai proses sel granulosa dan laju ovulasi. GDF9 merupakan OSF yang memainkan peran penting dalam menjaga fertilitas wanita. Penelitian ini bertujuan untuk mengetahui apakah ekspresi GDF9 berkorelasi dengan kualitas oosit dan laju fertilisasi pada pasien fertilisasi in vitro.
Tujuan: Mengetahui korelasi ekspresi GDF-9 dengan kualitas oosit dan laju fertilisasi pada pasien Fertilisasi In Vitro
Metode: Penelitian ini adalah penelitian potong lintang yang dilaksanakan di Klinik Yasmin, Rumah Sakit dr. Cipto Mangunkusumo, Jakarta sejak bulan Juli 2019 sampai bulan Juli 2020. Terdapat 26 wanita berusia 25-40 tahun pasien Fertilisasi In Vitro tanpa penyakit endometriosis maupun Sindrom Ovarium Polikistik yang menjadi subjek penelitian ini. Cairan folikel pasien diambil, kemudian dilakukan pengukuran ekspresi gen GDF9. Setelah itu, dilakukan evaluasi pada data ekspresi gen GDF9 serta diuji korelasinya dengan kualitas oosit dan laju fertilisasi pasien menggunakan aplikasi SPSS.
Hasil: Sebanyak 26 pasien IVF berpartisipasi dalam penelitian ini. Median jumlah ekspresi gen GDF9 adalah sebanyak 2.47 x 10-5 ng/μl dengan median kualitas oosit dan laju fertilisasi pasien IVF sebesar 3.00 dan 0.60. Berdasarkan hasil uji korelasi, terdapat korelasi negatif antara ekspresi GDF9 terhadap laju fertilisasi dengan kekuatan korelasi sedang (r = -0.443, p = 0.012). Sementara, ditemukan korelasi tidak bermakna antara ekspresi GDF9 terhadap kualitas oosit (r = -0.306, p = 0.064).
Kesimpulan: Terdapat korelasi negatif dengan kekuatan korelasi sedang antara ekspresi GDF9 dengan laju fertilisasi, sementara hubungan ekspresi GDF-9 dengan kualitas oosit memiliki korelasi tidak bermakna.

Background: Growth Differentiation Factor 9 (GDF9) is a member of the TGF-β superfamily which is one of the oocyte-secreted factor (OSF). GDF9 plays an important role in ovarian folliculogenesis, the competence of oocyte development, as well as an essential molecule that controls various granulosa cells process and the rate of ovulation. GDF9 is an OSF which plays an important role in maintaining female fertility. This study is conducted to see the correlation between the expression of GDF9 gene with the oocyte quality and the fertilization rate in the IVF patients.
Aim: To find out the correlation between the expression of GDF9 with the oocyte quality and the rate of fertilization of IVF Patients
Methods: This study is a cross-sectional study which was conducted at Klinik Yasmin dr. Cipto Mangunkusumo General Hospital, Jakarta since July 2019 to July 2020. 26 IVF patients aged 25-40 years without the conditions of endometriosis or Polycystic Ovary Syndrome (PCOS) participated in this study. Follicular fluids are taken from the patients and the GDF9 gene expression were measured. The data were then evaluated for its correlation with the oocyte quality and the fertilization rate using the SPSS application.
Results: Abour 26 IVF patients were participated in this study. The median amount of GDF9 gene expression was 2.47 x 10-5 ng/μl. The median of the oocyte quality and the fertilization rate was 3.00 and 0.60. According to the correlation test, there is a negative correlation between the expression of GDF9 gene and the fertilization rate with a moderate statistical correlation (r = -0.443, p = 0.012). Meanwhile, the expression of GDF9 gene has no significance correlation with the oocyte quality (r = -0.306, p = 0.064).
Conclusion: This study showed a negative correlation between the expression of GDF9 gene and the fertilizatio rate, while the relation between the GDF9 expression and the occyte quality has no significance correlation.
"
Lengkap +
Depok: Fakultas Kedokteran Universitas Indonesia, 2020
S-pdf
UI - Skripsi Membership  Universitas Indonesia Library
cover
Tiara Berliana Azzahra
"Latar belakang. Metode penilaian viabilitas embrio yang masih kurang akurat menjadi tantangan dalam peningkatan keberhasilan fertilisasi in vitro. Kualitas embrio dipengaruhi oleh umur kronologis dan regulator ekspresi gen. Salah satu regulator ekspresi gen tersebut merupakan MicroRNA-135b. MicroRNA-135b sangat potensial untuk dikembangkan lebih lanjut sebagai biomarker kualitas embrio fertilisasi in vitro
yang bersifat non-invasif. MicroRNA-135b terekspresi secara stabil di medium kultur embrio. Selain itu, terjadi peningkatan ekspresi MicroRNA-135b di medium kultur embrio aneuploidi dibandingkan medium kultur embrio euploidi pada fertilisasi in vitro. Kondisi aneuploidi pada embrio memiliki korelasi positif dengan umur kronologis dari pasien fertilisasi in vitro. Oleh karena itu, akan diteliti apakah terdapat korelasi antara umur kronologis dan ekspresi MicroRNA-135b pada medium kultur embrio pasien fertilisasi in vitro.
Tujuan. 1) Mengetahui sebaran umur kronologis dan ekspresi MicroRNA-135b pada pasien fertilisasi in vitro. 2) Mengetahui korelasi umur kronologis dan ekspresi MicroRNA-135b di medium kultur embrio pasien fertilisasi in vitro. Metode. Studi ini merupakan sebuah studi cross-sectional yang dilakukan pada pasien fertilisasi in vitro Klinik Yasmin RSCM Kencana. Data umur kronologis pasien diperoleh dari data rekam medis pasien. Sampel medium kultur embrio diambil di hari ke-5 prosedur kultur embrio. Selain itu, dilakukan juga pengambilan sampel medium basal sebagai kelompok kontrol. Sampel yang telah diambil akan diperiksa nilai ekspresi MicroRNA-135b dengan analisis kuantitatif real time PCR. Analisis data dilakukan dengan IBM SPSS Statistics 25. Hasil. Total sampel penelitian adalah sebanyak 31 medium kultur embrio dari 11 orang pasien. Umur kronologis dan ekspresi MicroRNA-135b tersebar secara tidak normal. Dari penelitian ini, ditemukan bahwa terdapat korelasi positif bermakna dengan kekuatan statistik sedang antara umur kronologis dengan ekspresi MicroRNA-135b di medium kultur embrio pasien fertilisasi in vitro. Selain itu, ditemukan pula peningkatan relatif ekspresi MicroRNA-135b sebesar 4,9 fold pada medium kultur embrio dibandingkan dengan medium basal fertilisasi in vitro.
Kesimpulan. Umur kronologis yang semakin meningkat diikuti dengan peningkatan ekspresi MicroRNA-135b di medium kultur embrio pasien fertilisasi in vitro.

Background. The lack of accuracy in embryo viability assessment methods still become a challenge to increase the in vitro fertilization (IVF) success rate. The quality of embryo
influenced by the chronological age and gene expression regulator. One of the gene expression regulator is MicroRNA-135b. MicroRNA-135b is very potential to become a
noninvasive biomarker of IVF embryo quality. MicroRNA-135b express stably in the spent embryo media. There is an increasement of MicroRNA-135b expression in aneuploidy spent embryo media than euploidy spent embryo media of IVF. Aneuploidy embryo has positive correlation with the IVF patient’s chronological age. Therefore, in
this study, we will determine whether chronological age has correlation with MicroRNA- 135b expression in spent embryo media of IVF patient. Objectives. 1) To determine the chronological age and MicroRNA-135b expression
distribution at IVF patient. 2) To determine the correlation between chronological age and MicroRNA-135b expression in spent embryo media of IVF patient.
Methods. This study is a cross-sectional study which was done to IVF patient of Yasmin Clinic in RSCM Kencana. The chronological age data were collected from the medical
records of the patient. The spent embryo media sample were taken at the 5th day of the spent embryo media procedure. We also collected the basal media sample as the control
group. The MicroRNA-135b expression were analysed using quantitative real time PCR analysis. The data analysis was using IBM SPSS Statistics 25.
Results. There were 31 spent embryo media from 11 patients. The chronological age and MicroRNA-135b expression distribute abnormal. We also found that there was a positive
significant correlation with moderate statistical power between chronological age and MicroRNA-135b expression in spent embryo media of IVF patient. We also found that
MicroRNA-135b expression increased 4,9 fold in spent embryo media than basal media of IVF. Conclusion. The increase of chronological age followed by the increase of MicroRNA-
135b expression in spent embryo media of IVF patient.
"
Lengkap +
Depok: Fakultas Kedokteran Univesitas Indonesia, 2020
S-pdf
UI - Skripsi Membership  Universitas Indonesia Library
cover
Veinardi Madjid
"Latar Belakang: Penggunaan klomifen sitrat sebagai obat induksi kehamilan masih memiliki angka keberhasilan kehamilan yang rendah. Letrozol merupakan agen penghambat aromatase yang dianggap memiliki efektivitas lebih baik dibanding klomifen sitrat, namun efektivitasnya masih dilaporkan bervariasi. Penelitian ini bertujuan untuk mengetahui perbedaan pemberian klomifen sitrat dan letrozol terhadap ketebalan endometrium, morfologi endometrium dan jumlah folikel dominan pada perempuan yang dilakukan induksi ovulasi atau stimulasi ovarium.
Metode: Penelitian ini merupakan penelitian retrospektif dengan menggunakan medis pasien yang dilakukan induksi ovulasi atau stimulasi ovarium pada Januari 2011 - Mei 2015. Didapatkan 143 wanita siklus anovulasi yang terbagi dalam empat kelompok: klomifen sitrat 50 mg, klomifen sitrat 100 mg, letrozol 2,5 mg dan letrozol 5 mg. Agen pemicu ovulasi pada subjek dimulai pada hari ke-2 selama berlangsung selama lima hari. Data ketebalan endometrium, morfologi endometrium dan jumlah folikel dominan didapat pada status dari data pemeriksaan ultrosonografi transvaginal di hari ke-12 siklus haid.
Hasil: Dari semua subjek, didapatkan 45 subjek (31,5%) mendapat klomifen sitrat 50 mg, 29 subjek (20,3%) dengan klomifen sitrat 100 mg, 23 subjek (16,1%) dengan letrozol 2,5 mg, dan 46 subjek (32,2%) dengan letrozol 5 mg. Subjek dengan letrozol memiliki endometrium yang lebih tebal dibandingkan dengan klomifen sitrat (p<0,05). Didapatkan pulan subjek dengan letrozol memiliki lebih banyak proporsi subjek dengan morfologi endometrium trilaminer. Tidak dijumpai perbedaan ketebalan endometrium pada subjek dengan perbedaan dosis pada masing-masing obat. Selain itu, tidak ditemukan perbedaan jumlah folikel dominan pada keempat kelompok.
Kesimpulan: Penggunaan letrozol menghasilkan endometrium yang lebih tebal dan endometrium trilaminer dibandingkan klomifen sitrat. Tidak dijumpai perbedaan jumlah folikel dominan pada kedua kelompok.

Background: The use of clomiphene citrate as an induction agent still has dissappointing results regarding its pregnancy rate. Letrozole is an aromatase inhibitor that is perceived to has better efficacy compared to clomiphene citrate, however, the reporting results were still varied. This study aimed to know the efficacy of clomiphene citrate and letrozol for ovulation induction in anovulation women.
Method: This was a retrospective study using medical recors of women undergone ovulation induction from January 2011-May 2015. A number of 143 anovulation women were divided into clomiphene citrate 50 mg, clomiphene citrate 100 mg, letrozol 2,5 mg and letrozol 5mg. Every group of ovulation induction agent recieved the agent daily on 3rd until 7th day menstrual cycle. On 12th menstrual cycle the transvaginal ultrasound was performed to measure endometrial thickness and dominant follicle number.
Results: From all subjects, 45 subjects (31,5%) were in 50 mg clomiphene citrate groups, 29 subjects (20,3%) in 100 mg clomiphene citrate group, 23 subjects (16,1%) in 2,5 mg letrozole group, and 46 subjects (32,2%) in 5 mg letrozole group. Subjects receiving letrozol had thicker endometrium compared to clomiphene citrate (p<0,05). Different doses did not associated with different endometrial thickness between subjects receiving either letrozole or clomiphene citrate. In addition, subjects receiving letrozole had higher proportion of having trilaminar endometrium morphology. We did not observe a difference in total number of dominant follicle between groups.
Conclusion: The usage of letrozol resulted in thicker endometrium and proportion of subjects with trilaminar endometrium. Yet, there is no difference in number of dominant follicle between groups.
"
Lengkap +
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2015
SP-Pdf
UI - Tugas Akhir  Universitas Indonesia Library
cover
Pramety Utami
"Hampir 50% kasus infertilitas disebabkan oleh faktor pria. Infertilitas pria dapat tidak terdeteksi dengan analisis sperma dan mempengaruhi keluaran Teknologi Reproduksi Berbantu. Penelitian ini bertujuan untuk mengembangkan metode pemeriksaan untuk meramalkan infertilitas pria. Dengan desain potong lintang dan consecutive sampling didapatkan 2 kelompok subjek, infertil (78 subjek) dan fertil (36 subjek). IFD sperma diperiksa menggunakan metode sperm chromatin dispersion (SCD) dengan kit Halosperm®. Didapatkan nilai median IFD sperma kelompok infertil lebih tinggi secara bermakna dibandingkan kelompok fertil. IFD sperma juga memiliki AUC yang paling tinggi dibandngkan ketiga komponen analisis sperma (konsentrasi, motilitas, dan morfologi). IFD sperma memiliki nilai diagnostik yang lebih tinggi dibandingkan analisis sperma dengan titik potong optimal 26,1% dengan sensitivitas 80,8%, spesifisitas 86,1%, NDP 92,6%, dan NDN 67,4%.

Almost 50% of infertility are caused by male factors. Male infertility could not be detected by conventional sperm analysis and affect the outcome of Assissted Reproductive Technology. This study aim to develop a method to predict male infertility better. Using cross-sectional design and consecutive sampling, obtained two groups of subjects, infertile (78 subjects) and fertile (36 subjects). Sperm DNA fragmentation index (DFI) was examined using sperm chromatin dispersion (SCD) test by Halosperm® kit. Median value of sperm DFI on infertile group was significantly higher compared to fertile group. Sperm DFI also had the highest AUC compared to the three components of conventional sperm analysis (concentration, motility, and morphology). Sperm DFI had a higher diagnostic value than the sperm analysis with optimal cut-off-point of 26.1% with sensitivity of 80.8%, specificity of 86.1%, PPV of 92.6%, and NPV of 67.4%."
Lengkap +
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2016
SP-pdf
UI - Tugas Akhir  Universitas Indonesia Library
cover
Intan Dyah Puspitasari
"Latar Belakang: Dengan tingginya angka kasus infertilitas, terdapat beberapa teknologi yang dapat digunakan sebagai tata laksana. Salah satunya adalah teknologi Fertilisasi In Vitro (IVF), yang dilakukan dengan menstimulasi pasien dengan hormon FSH untuk mengembangkan lebih banyak oosit dan mengambil oosit tersebut setelahnya untuk menginduksi fertilisasi dengan sel sperma. Kualitas oosit dievaluasi dengan mengukur ekspresi oocytes-secreting factors yang salah satunya adalah gen BMP15. Penelitian ini dilaksanakan untuk melihat korelasi antara dosis rFSH dan ekspresi gen BMP15 pada pasien IVF. Tujuan: Mengetahui korelasi dosis rekombinan FSH terhadap ekspresi gen BMP15 sel granulosa pada pasien Fertilisasi In Vitro. Metode: Penelitian ini merupakan penelitian potong lintang yang dilaksanakan di Klinik Yasmin, Rumah Sakit dr. Cipto Mangunkusumo, Jakarta sejak bulan Juli 2019 hingga bulan Juli 2020. Terdapat 20 wanita berusia 25-40 tahun tanpa endometriosis dan Sindrom Ovarium Polikistik yang menjadi subjek penelitian ini. Pada pasien, dilakukan pengambilan cairan folikel dan kemudian dilakukan pengukuran ekspresi gen BMP15 pasien. Data ekspresi gen BMP15 kemudian dievaluasi dan diuji korelasinya dengan dosis rFSH menggunakan aplikasi SPSS. Hasil: Sebanyak 17 pasien IVF berpartisipasi dalam penelitian ini. Median jumlah ekspresi gen BMP15 adalah sebanyak 6.49 x 10-10 ng/ml dan rata-rata dosis rFSH yang diberikan pada pasien adalah sebanyak 2660.29±165.24. Berdasarkan hasil uji korelasi, terdapat korelasi positif antara dosis rFSH dan ekspresi BMP15 dengan signifikansi sedang (r = 0.419, p = 0.047). Kesimpulan: Terdapat korelasi positif dengan signifikansi sedang antara dosis rFSH dan ekspresi gen BMP15.

Background: With the high number of infertility cases, there are technologies that are used as the management of infertility. One of them is in vitro fertilization, which is done by stimulating the patients to develop more oocytes using FSH hormones and taking the oocytes soon after to induce a fertilization with the sperm cells. We can evaluate the quality of the oocytes by measuring the expression of oocytes-secreting factors, one of which is the BMP15 gene. This study is conducted to see the correlation between the dose of rFSH and the expression of BMP15 genes in the IVF patients. Aim: To find out the correlation between the dose of recombinant FSH and the expression of BMP15 Gene in the Granulosa Cells of IVF Patients. Methods: A cross sectional study was held in Klinik Yasmin, Dr. Cipto Mangunkusumo General Hospital, Jakarta since July 2019 to July 2020. 20 women aged 25-40 years old without the conditions of endometriosis and PCOS participated in this study. Follicular fluids were taken from the patients and the BMP15 gene expression were measured. The data were then evaluated with the amount of rFSH using SPSS. Results: About 17 IVF patients were included in this study. The median amount of BMP15 gene expression was 6.49 x 10-10 ng/ml and the average amount of rFSH dose given to the patients was 2660.29±165.24. According to the correlation test, there was a positive correlation between the rFSH dose and the expression of BMP15 gene with medium significance (r = 0.419, p = 0.047). Conclusion: This study showed a positive correlation between the dose of rFSH given to the IVF patients and the expression of BMP15 gene in the granulosa cells."
Lengkap +
Depok: Fakultas Kedokteran Universitas Indonesia, 2020
S-pdf
UI - Skripsi Membership  Universitas Indonesia Library
<<   1 2 3 4 5   >>