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Abstrak :
Tujuan dari penelitian ini adalah untuk menganalisis pengaruh Platelet-Rich Plasma (PRP) Eksosom terhadap potensi regenerasi jaringan pulpa gigi dengan evaluasiin vitro viabilitas sel, aktivitas migrasi, dan ekspresi Vascular Endothelial Growth Factor-A (VEGF-A) sel punca pulpa gigi manusia (hDPSCs). HDPSC diambil darisembilan gigi molar tiga dari sembilan donor sesuai kriterian inklusi, dan isolasi serta kultur dilakukan dengan metode enzyme digestion (ED) yang dipanen antara P3 dan P4. Setelah starvatation, hDPSCs dikultur di dalam enam media, yaitu sebagai berikut: Dulbecco's Modified Eagle Medium (DMEM) dan 10% PRP sebagai kelompok kontrol, dan 0,5%, 1%, dan 5% PRP eksosom sebagai kelompok eksperimental. Semua kelompok memiliki tiga rangkap biologis (Triplo). Uji viabilitas sel dievaluasi dengan MTT assay, aktivitas migrasi sel dengan Scratch Assay dan Transwell Migration Assay, dan ekspresi VEGF-A dengan Enzyme- Linked Lmmunosorbent Assay (ELISA). Analisis data dilakukan dengan uji One Way ANOVA (p <0,05) serta uji Kruskal-Wallis dan post hoc Mann-Whitney (p<0,05). Nilai rata-rata viabilitas hDPSCs tertinggi pada 24, 48 dan 72 jam observasi pada kelompok PRP-Eksosom 5% (p <0,05). PRP Eksosom 5% menunjukkan aktivitas migrasi yang lebih tinggi dibandingkan dengan kelompok lain, meskipun tidak terdapat perbedaan bermakna dengan kontrol PRP 10% (p> 0,05). Ekspresi VEGF-A hDPSCs tertinggi terdapat pada kelompok PRP Eksosom 5% pada 72 jampengamatan. Dapat disimpulkan bahwa eksosom PRP 5% berpotensi menginduksi regenerasi pupa gigi manusia. ......The purpose of this study was to analyze the effect of Exosome Platelet-Rich Plasma (PRP) on their potential for human Dental Pulp regeneration by evaluating in vitro cell viability, migration activity, and expression of Vascular Endothelial Growth Factor-A (VEGF-A) of human Dental Pulp Stem Cells (hDPSCs). hDPSCs was taken from nine third molars from nine donors thatfit to the inclusion criteria of this study, isolation and culture were carried out by the enzyme digestion (EZ) method harvested between P3 and P4. After starvatation,hDPSCs were cultured in six media, namely as follows: Dulbecco's Modified EagleMedium (DMEM) and 10% PRP as the control group, and 0.5%, 1%, and 5% PRP exosomes as experimental groups. All groups had a biological triplication (Triplo). Cell viability test was evaluated by MTT assay, cell migration activity with Scratch Assay and Transwell Migration Assay, and VEGF-A expression by Enzyme- Linked Immunosorbent Assay (ELISA). Data analysis was performed using One Way ANOVA (p <0.05) and Kruskal-Wallis (p <0.05) and Pearson/Spearman Correlation test (p<0.05). The highest mean hDPSCs viability was at 24, 48 and 72 hours of observation in the PRP-exosome group 5% (p <0.05). Exosome PRP 5% showed higher migration activity compared to other groups, although there was no significant difference with PRP control 10% (p> 0.05). The highest expression of VEGF-A hDPSCs was found in the PRP exosome group 5% at 72 hours of observation. It can be concluded that the PRP 5% exosome have the highest potential ability in inducing pulp regeneration.

Abstrak :
Latar Belakang: Siler biokeramik berbasis kalsium silikat diketahui memiliki biokompabilitas tinggi, mampu beradhesi dengan dinding saluran akar serta dapat menginduksi respon osteogenik, yang dikategorikan sebagai material biomimetik. Akan tetapi, siler biokeramik memiliki kekurangan pada sifat fisiknya. Kitosan larut air merupakan bentuk modifikasi kitosan yang memiliki keunggulan sebagai antioksidan, antibakteri, berperan dalam penyembuhan lesi dan regenerasi jaringan serta sebagai dapat memperbaiki sifat fisik semen. Perpaduan kedua bahan dapat menciptakan semen saluran akar biomimetik. Tujuan: Melakukan uji karakteristik, sifat fisik dan biologis terhadap novel siler hibrida biokeramik-kitosan larut air (BCC) sebagai semen biomimetik saluran akar. Metode: Novel siler hibrida BCC dimanipulasi dari semen biokeramik (BC) yang telah melalui proses ball-milling dan sintering, kemudian dicampur kitosan larut air dengan W/P 0,4. Empat variabel penelitian, yaitu semen biokeramik BC, novel siler hibrida BCC, siler biokeramik dan siler epoksi resin dilakukan uji karakterisasi (XRD, SEM/EDS), uji sifat fisik (setting time, daya alir, film thickness), serta uji biologis (sitotoksisitas dan bioaktivitas terhadap hPDLSCs). Hasil: Novel siler hibrida BCC mengandung unsur dan puncak kristalin yang serupa dengan siler biokeramik (Sure-Seal Root™), memiliki bentuk partikel yang cenderung globular dan homogen dengan jarak antarpartikel lebih rapat serta ukuran partikel deskriptif yang lebih besar dibandingkan Sure-Seal Root™ dan AH Plus® tetapi lebih kecil dibandingkan semen biokeramik BC. Hanya semen biokeramik BC dan novel siler BCC yang memiliki rasio Ca/Si/P. Novel siler BCC menunjukkan perbedaan setting time, daya alir dan film thickness yang bermakna dengan semen biokeramik BC, Sure-Seal Root™ dan AH Plus®. Keempat kelompok menunjukkan sitotoksisitas rendah terhadap hPDLSCs. Bioaktivitas novel siler BCC relatif lebih tinggi dibandingkan semua kelompok perlakuan dan kontrol. Kesimpulan: Novel siler hibrida BCC berpotensi memberikan implikasi klinis yang baik, menunjukkan sifat fisik yang mendekati standar semen saluran akar dan memiliki bioaktivitas sebagai semen biomimetik saluran akar. ......Background: Currently, bioceramic sealers with calcium silicate based have been developed and show high biocompatibility, are able to adhere to root canal dentin and can induce an osteogenic response, which can be categorized as biomimetic materials. On the other hand, water-soluble particle (WSP) chitosan, one of chitosan derivatives, has advantages as an antioxidant, antibacterial, plays a role in wound healing and tissue regeneration as well as a thickening agent. The combination of these two materials can create a biomimetic endodontic sealer. Objective: To examine the characteristics, physical and biological properties of novel bioceramic-chitosan hybrid sealer (BCC) as biomimetic endodontic sealer. Methods: Novel BCC sealer were manipulated from bioceramic cement (BC) which had been synthesized through a ball-milling and sintering process, then mixed with WSP chitosan with a W/P of 0,4. Four variables, namely novel BCC sealer, BC bioceramic cement, bioceramic and epoxy resin sealer were tested characterization (XRD, SEM/EDS), physical properties (setting time, flow, film thickness), and biological tests (cytotoxicity and bioactivity on hPDLSCs). Results: Novel BCC sealer showed bioceramic elements and crystalline peaks similar to bioceramic sealer (Sure-Seal Root™), had a particle shape that tends to be globular and homogeneous with descriptive particle size larger than Sure-Seal Root™ and AH Plus® but smaller compared to BC bioceramic cement. Only BC bioceramic cements and novel BCC sealer had Ca/Si/P ratios. Novel BCC sealer showed significant differences in setting time, flow, and film thickness with BC bioceramic cements, Sure-Seal Root™ and AH Plus®. The four groups showed low cytotoxicity to hPDLSCs. The bioactivity of novel BCC sealer was relatively higher among other groups and control group. Conclusion: Novel BCC hybrid sealer has good clinical implications, exhibit physical properties close to standard root canal cements and have bioactivity as root canal biomimetic sealer.