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Abstrak :
Latar Belakang: Resin komposit single-shade merupakan resin komposit yang dapat menghasilkan warna menyerupai berbagai shade gigi tanpa tambahan pigmen. Resin komposit single-shade tetap memiliki potensi perubahan warna saat terpapar zat pewarna. Teh hitam dan oolong memiliki kadar tanin yang dapat mempengaruhi stabilitas warna resin komposit. Maka, dilakukan pengujian perubahan warna resin komposit single-shade setelah perendaman dalam larutan teh hitam dan oolong. Tujuan: Mengetahui perbedaan perubahan warna antara resin komposit single-shade yang direndam dalam larutan teh hitam dan oolong. Metode: Spesimen resin komposit single-shade dan konvensional nanohybrid (n = 42) dibagi ke dalam 6 kelompok, kemudian direndam dalam larutan teh hitam dan oolong selama 24 jam/hari dalam waktu 7 hari. Pengukuran perubahan warna dilakukan dengan colorimeter. Hasil: Perendaman dalam larutan teh hitam menghasilkan perubahan warna yang tidak sesuai pada resin komposit single-shade. Terdapat perbedaan perubahan warna signifikan antara resin komposit konvensional dalam kedua larutan teh, serta antara resin komposit single-shade dalam kedua larutan teh (p < 0.05). Kesimpulan: Perendaman resin komposit dalam teh hitam atau oolong menyebabkan perubahan warna resin komposit konvensional dan single-shade. Teh hitam menyebabkan perubahan warna lebih besar dibandingkan teh oolong pada kedua jenis resin komposit. ......Single-shade composite resin is a composite resin that produces various teeth shades without additional pigments. Single-shade composite resin still has its color change potential when exposed to colorants. Black and oolong tea possess tannin contents that influence composite resin’s color stability. Therefore, single-shade resin composite’s color change was evaluated after its immersion in black and oolong tea solutions. Objective: To determine color change difference of single-shade composite resin after its immersion in black and oolong tea solutions. Methods: Single-shade and conventional nanohybrid composite resin specimens (n = 42) were divided into 6 groups, then immersed in black and oolong tea solutions for 24 hours/day for 7 days. Color change measurements were taken with a colorimeter. Results: Immersion in black tea resulted in unacceptable color change in single-shade composite resin. Significant difference in color change was found between conventional composite resin immersed in black and oolong tea, and between single-shade composite resin immersed in black and oolong tea (p < 0.05). Conclusions: Black and oolong tea immersion causes color change in conventional and single-shade composite resins. Black tea causes larger color change compared to oolong tea in both composite resins.

Abstrak :
Latar Belakang : Nanosilika sekam padi diekstraksi melalui metode sol-gel dan pirolisis yang memiliki struktur amorf, berpori, dan permukaannya mengandung gugus silanol (Si-OH). Interaksi gugus silanol (Si-OH) dengan ion kalsium membentuk kristal apatit. Tujuan : Penelitian ini bertujuan untuk mengetahui efek nano silika sekam padi metode sol-gel dan pirolisis terhadap peningkatan jumlah hidroksiapatit dentin. Metode : 12 sampel kavitas dibagi menjadi 4 kelompok. Kelompok 1 (dentin normal) sebagai kontrol, kelompok 2 dentin demineralisasi, kelompok 3 dentin demineralisasi diaplikasi nanosilika sekam padi metode sol-gel, dan kelompok 4 dentin demineralisasi diaplikasi nanosilika sekam padi metode pirolisis. Kemudian seluruh sampel disimpan dalam shaking incubator pada suhu 37°C. Selanjutnya dilakukan pemeriksaan dengan XRD untuk melihat derajat kristalinitas hidroksiapatit. Hasil : Terdapat peningkatan derajat kristalinitas hidroksiapatit dentin setelah aplikasi nanosilika sekam padi metode pirolisis dan sol-gel yang nilainya tidak berbeda bermakna dengan kelompok dentin normal (kontrol). Kesimpulan: Nanosilika sekam padi metode sol-gel dan pirolisis mempunyai kemampuan yang sama dalam meningkatkan jumlah kristal hidroksiapatit dentin. ......Background: Rice husk nanosilica is a material extracted through sol-gel and pyrolysis methods, has amorphous, porous, and contain silanol (Si-OH) groups on their surface. The silanol group (Si-OH) interacting with calcium ions will induce the formation of apatite crystals. Objective: To determine the effect of rice husk nanosilica sol-gel and pyrolysis methods on incresed amount of dentin hydroxyapatite. Methods: 12 cavity samples were divided into 4 groups. Group 1 (normal dentin) as control group, group 2 is a demineralized dentin group, group 3 is a demineralized dentin group applied to rice husk nanosilica through sol-gel method, and group 4 is a demineralized dentin group applied to rice husk nanosilica through pyrolysis method. All samples are then kept inside a shaking incubator at a temperature of 37°C. Next an examination was done using an XRD to see the degree of hydroxyapatite crystallinity Result: There is an increase degree of crystallinity of dentin hydroxyapatite, after the application of rice husk nanosilica and sol-gel and pyrolysis methods whose value was not significantly different from the normal dentin (control) group. Conclusion: Rice husk nanosilica sol-gel and pyrolysis methods have the same capability to increase the amount of dentin hydroxyapatite crystals.

Abstrak :
lingkungan kondusif untuk proses penyembuhan. Biological sealing dapat diperoleh melalui sifat bioaktivitas suatu material, salah satunya kemampuan biomineralisasi material, yaitu kemampuan material dalam membentuk apatite like layer pada permukaan ketika berkontak dengan cairan fisiologis. Karakteristik tersebut dapat diperoleh dari material bioaktif, seperti kalsium silikat. Siler berbasis kalsium silikat pre- mixed saat ini telah banyak berkembang, di antaranya adalah Ceraseal® (Metabiomed, Korea) dan AH Plus® Bioceramic (Dentsply, USA). Komposisi masing-masing siler yang bervariasi menghasilkan perbedaan karakteristik, salah satunya kemampuan biomineralisasi. Tujuan: Menganalisis potensi biomineralisasi antara siler berbasis kalsium silikat pada dentin saluran akar dengan perendaman phosphate buffered saline (PBS). Metode: Siler berbasis kalsium silikat Ceraseal® dan AH Plus® Bioceramic diaplikasikan ke dalam dentin saluran akar gigi premolar yang telah dilakukan prosedur preparasi saluran akar, dan dilanjutkan dengan perendaman dalam PBS selama 14 hari. Analisis biomineralisasi dilakukan dengan menganalisis pembentukan lapisan apatit setelah 14 hari perendaman melalui penghitungan ketebalan deposit lapisan apatit menggunaan Scanning Electron Microscope (SEM), serta peningkatan pH larutan yang dihitung pada waktu observasi hari-ke 0, 7 dan 14 menggunakan pH-meter. Hasil: Terdapat perbedaan deposisi apatit pada interfacial layer antara Ceraseal® dan AH Plus® Bioceramic dalam waktu observasi 14 hari. Terdapat perbedaan bermakna antara nilai pH Ceraseal® dan AH Plus® Bioceramic pada waktu observasi 7 dan 14 hari. ......Background: Endodontic treatment currently refers to the concept of biological sealing to form an environment conducive to the healing process. Biological sealing can be obtained through the bioactivity properties of a material, one of which is its biomineralization ability to form an apatite-like layer on the surface when in contact with physiological fluids. This characteristic is present in bioactive materials like calcium silicate. There are currently many developed pre-mixed calcium silicate based sealers, including Ceraseal® (Metabiomed, Korea) and AH Plus® Bioceramic (Dentsply, USA). The variation in their composition results in different characteristics, including biomineralization ability. Objective: To analyze the biomineralization potential of calcium silicate-based sealers on root canal dentine by phosphate-buffered saline (PBS) immersion. Methods Calcium silicate based sealer Ceraseal® and AH Plus® Bioceramic were applied to the root canal dentin of premolar teeth that had undergone root canal preparation procedures. The samples were then immersed in PBS for 14 days. Biomineralization analysis was performed by measuring the apatite layer thickness formed after 14 days of immersion using a Scanning Electron Microscope (SEM). Additionally, the pH of the solution was measured at observation times of 0,7 and 14 days using a digital pH-meter. Results: There was a significant differences in in apatite deposition at the interfacial layer between Ceraseal® and AH Plus® Bioceramic during 14 days of observation. Furthermore, there was a significant difference in pH values between Ceraseal® and AH Plus® Bioceramic at 7 and 14 days of observation.

Abstrak :
Latar Belakang: L-arginin merupakan asam amino semiesensial yang produksinya tidak mencukupi kebutuhan dalam kondisi stres oksidatif akibat inflamasi. L-arginin adalah satu-satunya substrat bagi enzim nitric oxide synthase (NOS) yang memproduksi nitric oxide (NO) yang dapat mengaktivasi focal adhesion kinase (FAK) pathwaydan memicu terjadinya proses migrasi sel. Tujuan: Mengetahui potensi media kultur asam amino L-arginin terhadap laju kecepatan migrasi hDPSCs. Metode: Evaluasi media kultur asam amino L-arginin konsentrasi 300, 400, 500 ¼mol/L, serta DMEM sebagai kontrol terhadap laju kecepatan migrasi hDPSCs menggunakan uji scratch assay menggunakan uji scratch assay yang dihitung dengan rumus laju kecepatan migrasi setelah 24 jam. Analisis statistic menggunakan Paired T-Test dan Oneway ANOVA dengan post hoc LSD. Hasil: Terdapat perbedaan bermakna potensi L-arginin 500 μmol/L dibandingkan konsentrasi 300 dan 400 μmol/L, serta kontrol. Kesimpulan: Media kultur asam amino L-arginin 500 ¼mol/L memiliki potensi laju kecepatan migrasi yang lebih baik dibandingkan konsetrasi 300, 400 ¼mol/L dan kontrol. ...... Background: L-arginine is semiessential amino acid which the production is insufficient under oxidative stress due to inflammation. L-arginine is the only substrate of nitric oxide synthase (NOS) enzyme that produces nitric oxide (NO) which activates focal adhesion kinase (FAK) pathway to stimulate cell migration. Objective: To understand potential of L-arginine amino acid culture media towards speed rate of hDPSCs migration. Methods: Evaluation of 300, 400, 500 ¼mol/L of L-arginin amino acid culture media and DMEM as control towars speed rate of hDPSCs migration using scratch assay and calculation of migration speed rate after 24 hours. Statistical analysis using Paired T-Test and Oneway ANOVA with post hoc LSD. Results: Significant result was shown between 500 ¼mol/L of L-arginin amino acid culture media compared with 300 and 400 ¼mol/L concentration and control towards migration speed rate after 24 hours. Conclusion: 500 ¼mol/L of L-arginin amino acid culture media has a better migration rate compared with lower concentrations and control.

Abstrak :
Latar Belakang: Pada kondisi inflamasi pulpa, terdapat penurunan dari jumlah protein dan asam amino. L-Arginine adalah asam amino semi-esensial karena berperan penting pada kondisi tertentu, gangguan imun berat dan luka bakar, yang membutuhkan asupan tambahan L-Arginine eksternal. Asam amino L-Arginine menjadi satu-satunya substrat sintesis nitric oxide (NO) dan poliamina berasal dari konversi L-Arginine menjadi ornithinen melalui arginase. NO dan poliamina merangsang proliferasi sel dan memiliki efek positif pada perkembangan melalui siklus sel. Tujuan: Mengetahui potensi asam amino L-Arginine terhadap proliferasi hDPSCs. Metode: Evaluasi asam amino L- Arginine konsentrasi 300, 400, 500 μmol/L, serta DMEM sebagai kontrol terhadap proliferasi hDPSCs menggunakan uji cell count setelah 24 jam. Analisis statistic menggunakan Oneway ANOVA dengan post hoc Bonferroni. Hasil: Terdapat perbedaan bermakna potensi L-Arginine 300,400 dan 500 μmol/L dibandingkan kontrol, dan L- Arginine 500 μmol/L memiliki rerata proliferasi hDPSCs paling tinggi sebesar 436.666 sel/ml. Kesimpulan: Asam amino L-Argininee memiliki potensi terhadap proliferasi hDPSCs dan proliferasi tertinggi pada asam amino L-Arginine konsentrasi 500 μmol/L. ......Background: In the inflammatory condition of the pulp, there is a decrease in the amount of protein and amino acids. L-Arginine is a semi-essential amino acid because it plays an important role in certain conditions, severe immune disorders and burns, which require additional intake of external L-Arginine. The amino acid L-Arginine is the sole substrate for the synthesis of nitric oxide (NO) and polyamines derived from the conversion of L- Arginine to ornithine via arginase. NO and polyamines stimulate cell proliferation and have a positive effect on progression through the cell cycle. Objective: To determine the potential of L-Arginine amino acid on the proliferation of hDPSCs. Methods: Evaluation of L-Arginine amino acid with concentrations of 300, 400, 500 μmol/L, and DMEM as a control for hDPSCs proliferation using cell count test after 24 hours. Statistical analysis using Oneway ANOVA with Bonferroni post hoc. Results: There was a significant difference in the potency of L-Arginine 300,400 and 500 μmol/L compared to control, and L-Arginine 500 mol/L had the highest average proliferation of hDPSCs of 436.666 cells/ml. Conclusion: The amino acid L-arginine has the potential to proliferate hDPSCs and the highest concentration at 500 μmol/L.