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Abstrak :
The application of fish recombinant growth hormone (rGH) has been known as one of the methods to improve the growth performance of cultured fishes, one of which is the striped catfish Pangasianodon hypophthalmus, a species that is becoming commercially attractive in Indonesia. Hence, this study was aimed to evaluate the effects of rGH supplementation in commercial diet on the growth, feed utilization and flesh quality in P. hypophthalmus grow out. The rGH was mixed with chicken egg yolk and sprayed on the commercial feeds with different protein levels (32, 28 and 23%). In the control, the feeds were also sprayed with chicken egg yolk but without rGH. Striped catfish with body weights of 110.66 ± 1.32 g ind-1 were fed on rGH-supplemented diets two times a week during the first and third months, and during the rest of the months they were fed on diet without rGH supplementation. The fishes were reared for 120 days in 18 hapa (2×1×1.5 m3) with initial density of 20 fishes per hapa. The result showed that the highest weight gain, specific growth rate (SGR), and lowest feed conversion ratio (FCR) were obtained by fishes fed on 32% protein content with rGH-supplemented diet. No significant difference was observed in the weight gain, SGR and FCR in rGH treated group with 28% protein content with rGH supplemented diet and non-rGH control group receiving 32% protein diet. Similar moisture content of meat, protein content of meat, belly fat and edible portion were observed in rGH-supplemented diet and their control. Except in the treatment 23% protein content rGH supplemented diet that has lower lipid content in fish body and meat. The highest SGR was obtained when the fishes were fed on the 32% protein feed combined with rGH. Enrichment with rGH depleted the fat content in the meat of fish fed on all levels of protein in which the lowest fat was found in the 23% protein feed

Abstrak :
Latar Belakang: Luka bakar masih menjadi masalah kesehatan yang berat khususnya di Indonesia. Pada kasus luka bakar mayor, penutupan luka sementara dengan menggunakan xenograft terbukti memberikan keuntungan. Akan tetapi tidak semua jenis xenograft tersedia akibat latar belakang kultur, biaya, dan agama disamping tampilan bersisik pada jenis xenograft ikan tilapia yang kurang estetik. Patin siam (Pangasius hypophthalmus) adalah ikan tidak bersisik yang memiliki banyak kandungan kolagen tipe I. Studi ini bertujuan untuk melakukan komparasi kulit ikan patin siam terhadap kulit ikan tilapia dan babi yang telah umum dijadikan material xenograft pada luka bakar. Metode: Studi ini merupakan studi eksperimental menggunakan sembilan sampel berbeda dari kulit ikan patin siam, ikan tilapia, dan babi. Setiap sampel dilakukan preparasi dan dilakukan evaluasi secara histologi dengan menggunakan pewarnaan hematoxylin-eosin stained. Dilakukan dokumentasi dan analisa pada tampilan makroskopik dan mikroskopik setiap sampel. Hasil: Tampilan makroskopik kulit ikan patin siam menggambarkan kulit yang tidak berbulu, tidak bersisik, berwarna hitam – perak, dan memiliki ketebalan yang moderat. Tampilan mikroskopik kulit ikan patin siam memiliki ketebalan epidermis (8.49±1.60 μm) yang berbeda secara signifikan terhadap ikan tilapia (2.18±0.37 μm; p<0.001) dan babi (42.22±14.85 μm; p=0.002). Ketebalan dermis kulit ikan patin siam (288.46±119.04 μm) menyerupai ikan tilapia (210.68±46.62 μm; p=0.783) namun berbeda signifikan terhadap babi (1708.44±505.12 μm; p<0.001). Integritas dan susunan kolagen ikan patin siam serupa dengan tilapia berdasarkan penilaian histologi semi-kuantitatif (p>0.05). Kesimpulan: Ikan patin siam memiliki tampilan makroskopik dan tampilan mikroskopik yang dapat dibandingkan dengan ikan tilapia; tampilan makroskopik lebih halus, epidermis lebih tebal, dan tebal dermis yang serupa. Oleh karena itu, kulit ikan patin siam dipercaya dapat menjadi materi xenograft. Studi lanjutan diperlukan untuk mengevaluasi efektivitas dan kelayakan xenograft patin siam dalam tata laksana luka bakar. ......Background: Burn injury remains a health problem, specifically in Indonesia. In major burns, xenograft had been proved to be useful as temporary wound coverage. However, some xenografts are not widely available due to cultural, financial, and religious backgrounds or have unesthetic appearance, such as scaly appearance of tilapia fish xenograft. Striped catfish (Pangasius hypophthalmus) is a scaleless fish that has abundant type 1 collagen. This study aimed to compare striped catfish skin to commonly used xenograft (Nile tilapia and porcine skin) as xenograft material for burn wound. Methods: In this experimental study, nine different skin samples of striped catfishes, Nile tilapias, and porcines were prepared and histologically examined using hematoxylin- eosin stained samples. Macroscopic and microscopic features of each samples were documented and analysed. Results: The macroscopic skin appearances of striped catfishes were hairless and scaleless with black-silver color and moderate thickness. As for microscopic features, the epidermal thickness of striped catfish’s skin (8.49±1.60 μm) was significantly different to both Nile tilapia (2.18±0.37 μm; p<0.001) and porcine skin (42.22±14.85 μm; p=0.002). The dermal thickness of striped catfish’s skin (288.46±119.04 μm) was similar to Nile tilapia (210.68±46.62 μm; p=0.783) but differs significantly to porcine skin (1708.44±505.12 μm; p<0.001). The integrity and collagen organization of striped catfishes was also similar to tilapia based on semi-quantitative histology scoring system (p>0.05). Conclusion: Striped catfishes had potential macroscopic appearance and comparable microscopic features to Nile tilapia; smoother macroscopic appearance, thicker epidermis, and similar dermis thickness. Therefore, we believe it can be potentially used as a xenograft material. Further studies are required to evaluate the effectiveness and feasibility of striped catfish xenograft in burn wound management.