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Abstrak :
[ABSTRAK
Latar Belakang: Peran root surface conditioning terhadap keberadaan smear layer. Tujuan: Menganalisis hubungan antara penggunaan root surface conditioning (minosiklin 2,1% dan EDTA 24%) terhadap keberadaan smear layer setelah penghalusan akar gigi. Metoda: Sepuluh gigi manusia yang dicabut akibat kelainan periodontal dan dilakukan penghalusan akar. Gigi dipotong pada daerah sepertiga servikal, dan 30 spesimen yang terbentuk dibagi dalam tiga kelompok. Hasil: Tidak ada perbedaan signifikan terhadap tingkat keberadaan smear layer antara kelompok minosiklin maupun EDTA (p=0,759). Terdapat perbedaan signifikan antara kelompok minosiklin dan EDTA dengan salin sebagai kontrol (p=0,00). Kesimpulan: Ada hubungan antara penggunaan root surface conditioning terhadap keberadaan smear layer.

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ABSTRACT
Background: Role of root surface conditioning of the existence of smear layer. Objective: To analyze the smear layer on root surface conditioned with minocycline HCl 2,1% , EDTA gel 24%, after root planed. Materials and methods: Ten human teeth removed due to chronic periodontitis were collected and root planed. The teeth were sectioned on 1/3 cervikal, 30 specimens were divided into three groups. Results: No significant differences of smear layer between minocycline and EDTA (p=0,759). There was significant differences of minocycline and EDTA group compare to saline (p=0,00). Conclusion: There was relationship of root surface conditioning treatment with smear layer.;Background: Role of root surface conditioning of the existence of smear layer. Objective: To analyze the smear layer on root surface conditioned with minocycline HCl 2,1% , EDTA gel 24%, after root planed. Materials and methods: Ten human teeth removed due to chronic periodontitis were collected and root planed. The teeth were sectioned on 1/3 cervikal, 30 specimens were divided into three groups. Results: No significant differences of smear layer between minocycline and EDTA (p=0,759). There was significant differences of minocycline and EDTA group compare to saline (p=0,00). Conclusion: There was relationship of root surface conditioning treatment with smear layer., Background: Role of root surface conditioning of the existence of smear layer. Objective: To analyze the smear layer on root surface conditioned with minocycline HCl 2,1% , EDTA gel 24%, after root planed. Materials and methods: Ten human teeth removed due to chronic periodontitis were collected and root planed. The teeth were sectioned on 1/3 cervikal, 30 specimens were divided into three groups. Results: No significant differences of smear layer between minocycline and EDTA (p=0,759). There was significant differences of minocycline and EDTA group compare to saline (p=0,00). Conclusion: There was relationship of root surface conditioning treatment with smear layer.]

Abstrak :
Kromosom merupakan substansi genetik pada makhluk hidup yang diwariskan ke generasi selanjutnya. Kromosom terbentuk melalui proses kondensasi selama siklus sel. Faktor-faktor yang mempengaruhi kondensasi kromosom adalah protein scaffold dan kation divalen, terutama ion kalsium (Ca2+). Peran ion kalsium terhadap kromosom masih terbatas pada kromosom manusia dan belum pernah dilaporkan pada kromosom tumbuhan. Tujuan penelitian ini untuk mengetahui peran ion kalsium terhadap struktur kromosom barley menggunakan mikroskop fluoresens dan scanning electron microscope. Kromosom barley diisolasi kemudian diberi perlakuan 1 mM BAPTA sebagai agen pengikat ion kalsium dan PBS sebagai kontrol. Terdapat perbedaan panjang dan struktur kromosom barley setelah diberikan perlakuan 1 mM BAPTA dibandingkan dengan kromosom kontrol. Kromosom kontrol memiliki panjang rata-rata 5,3 μm dengan struktur kromosom yang padat, sedangkan kromosom dengan perlakuan BAPTA memiliki panjang rata-rata 10,7 μm dengan struktur kromosom lebih renggang dan kurang padat. Hasil tersebut menunjukkan bahwa ion kalsium memiliki peranan penting dalam menjaga struktur kromosom barley. ......Chromosomes are genetic substances in living organisms that are passed on to the next generation. Chromosomes are formed through the process of condensation during the cell cycle. Factors that influence chromosome condensation are scaffold proteins and divalent cations, especially calcium ions (Ca2+). The role of calcium ions on chromosomes is still limited to human chromosomes and has never been reported on plant chromosomes. The purpose of this study was to determine the role of calcium ions on the structure of barley chromosomes using a fluorescence microscope and scanning electron microscope. The barley chromosome was isolated and then treated with 1 mM BAPTA as a calcium ion chelating agent and PBS as a control. According to the data obtained, there are differences in length and structure of barley chromosomes after being treated with 1 mM BAPTA compared to control chromosomes. The control chromosome has an average length of 5.3 μm with a compact chromosome structure and chromosomes with BAPTA treatment have an average length of 10.7 μm with a less condense chromosome structure. These results indicated that calcium ions have an important role on maintaining the structure of barley chromosomes.

Abstrak :
Di tengah wabah Tuberkulosis TB, penelitian tentang obat tuberkulosis baru yang dapat memberikan resolusi penyembuhan lebih cepat sangatlah penting. Penemuan baru tersebut berperan penting dalam menurunankan jumlah pasien yang terjangkit wabah TB di seluruh dunia. Efektivitas rifapentin telah dinilai dan telah terbukti menjadi antibiotik yang paling efektif untuk menyembuhkan TB. Penggunaan rifapentin yang sering dengan dosis yang tinggi dapat menghasilkan resolusi penyembuhan yang lebih cepat. Namun, berdasarkan penelitian terdahulu, tingkat penggunaan yang sering tidak dapat dicapai secara oral melainkan dalam bentuk aerosol sehingga langsung menuju ke paru-paru yang terinfeksi. Oleh karena itu, dengan meningkatnya minat terhadap efektivitas rifapentin, penelitian ini mengintegrasikan bentuk hirup dari kristal rifapentin yang diperoleh dengan menggunakan metode kristalisasi dengan penambahan anti-pelarut dalam sistem batch dengan menggunakan aseton sebagai pelarut dan air suling sebagai anti-pelarut. Selain itu, penelitian ini menyelidiki mengenai pengaruh jumlah benih seed , rasio supersaturasi, dan waktu pengkristalan terhadap karakterisasi produk kristal yang dihasilkan agar dapat diperoleh ukuran yang sesuai. Berdasarkan penelitian terhadap masing-masing pengaruh, diperoleh hasil optimal pada penelitian tanpa menggunakan benih unseeded dengan rasio supersaturasi = 1.26. ......In the midst of Tuberculosis pandemic, a research about new tuberculosis drug that results in more rapid resolution of tubercular infection is important. It will play a crucial role in accelerating the reductions in tuberculosis incidence that is occurring worldwide. The effectiveness of rifapentine has been assessed and it has been proven to be the most effective antibiotics for Tuberculosis. A frequent administration and dose of rifapentine resulted in more rapid resolution of tubercular infection. However, based on former research, high exposure levels for treatment shortening may be unachievable with oral administration and might instead be achieved by direct aerosol delivery of rifapentine to the pulmonary site of infection. Therefore, with the growing interest toward the effectiveness of rifapentine in frequent administration and dose, this research integrates an inhalable form of crystalline rifapentine prepared using anti solvent batch crystallization method with acetone as a solvent and distilled water as an anti solvent. Moreover, this research investigates the effect of seed loading, supersaturation ratio, and residence time on the characterization of crystalline rifapentine in order to form a crystalline rifapentine in an inhalable size. Based on the assessment of each effect, optimum result was obtained at unseeded experiment with supersaturation ratio 1.26.

Abstrak :
Kondensasi kromosom pada tahap metafase memiliki peranan penting dalam keberlangsungan siklus sel. Sejauh ini masih terus dilakukan penelitian mengenai faktor yang mempengaruhi kondensasi kromosom. Kation divalen, terutama kalsium (Ca2+) diketahui memiliki peranan penting dalam kondensasi kromosom manusia. Akan tetapi, peranan Ca2+ terhadap kromosom tumbuhan belum diketahui. Gandum merupakan salah satu organisme model dalam sitogenetika. Oleh karena itu, diperlukan penelitian mengenai efek Ca2+ kalsium terhadap kondensasi kromsosom gandum (Triticum spp.). Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian 1 mM BAPTA (1, 2-bis(2-aminophenoxy) ethane-N, N, N′, N′-tetraacetic acid), 1 mM EDTA (Ethylenediaminetetraacetic acid), sebagai pelekat Ca2+, terhadap struktur kromosom gandum menggunakan mikroskop cahaya dan Scanning Electron Microscope (SEM). Kecambah gandum dipotong bagian ujung akar dan diinkubasi di dalam larutan PFA (Paraformaldehyde) 2%. Sampel diinkubasi dengan enzim selulase 2,5 % dan pectoliase 2,5%. Setelah itu sampel diberi perlakuan masing-masing dengan 1 mM BAPTA, 1 mM EDTA, dan kontrol dengan PBS. Kromosom kemudian diwarnai dengan aceto orcein sedangkan yang diamati SEM dipreparasi melalui tahap fiksasi, post-fiksasi, staining, dehidrasi, dan dikeringkan dengan HMDS (hexamethyldisilazane) sebelum diamati menggunakan SEM. Hasil yang diperoleh pada pengamatan kuantitatif menunjukkan panjang rata-rata untuk kromosom kontrol 12,5±3,4 µm sedangkan pada perlakuan 1 mM BAPTA dan 1 mM EDTA panjang rata-rata yang diperoleh adalah 17,7±6,6 µm dan 41,9±16,3 µm. Struktur kromosom kontrol secara kualitatif lebih padat dan lebih terkondensasi. Akan tetapi kromosom yang diberi perlakuan 1 mM BAPTA dan 1 mM EDTA menunjukkan hasil yang lebih panjang, tersebar, dan terjadi dekondensasi kromosom sehingga serat-serat kromatin lebih terlihat jelas. Hal tersebut menunjukkan Ca2+ memiliki peranan penting dalam kondensasi kromosom gandum. ......Chromosome condensation at metaphase has an important role in the continuation of the cell cycle. Researches on the investigation of the major factors affecting chromosome condensation has been conducted so far. Divalent cations, especially Ca2+ has been reported to have an important role in human chromosome condensation. Nevertheless, its effect on the plant chromosome has yet to be evaluated. Wheat (Triticum spp.) is one of the model organisms in cytogenetics.Therefore, study to evaluate the effect of Ca2+ on wheat chromsosomes is required. The purpose of this study was to determine the effect of 1 mM BAPTA and 1 mM EDTA as the Ca2+ chelating agent on the wheat chromosomes using light microscope and SEM (Scanning Electron Microscope). The root tips of wheat sprouts was cut off and incubated in a 2% PFA (Paraformaldehyde)  solution. The sample was incubated with 2.5% cellulase and 2.5% pectoliase enzyme. After that, the samples were treated with 1 mM BAPTA, 1 mM EDTA, and PBS as control. Chromosome were then stained with aceto orcein, while sample for SEM were subjected to SEM preparation including fixation, post-fixtaion, staining, dehydration, and HMDS (hexamethyldisilazane)  drying. The results showed that the average length of the control chromosome was 12.5±3.4 µm, while those treated with 1 mM BAPTA and 1 mM EDTA showed the average length of 17.7 ± 6.6 µm and 41. 9 ± 16.3 µm, respectively. The qualitative observation of the control chromosomes showed the more compact and condensed structure. However, chromosomes treated with 1 mM BAPTA and 1 mM EDTA showed a longer, dispered, and decondensed chromosome showing the chromatin fibers. This result indicated that Ca2+ play an important role in wheat chromosome condensation.

Abstrak :
Kanker serviks merupakan salah satu jenis kanker yang paling banyak diderita wanita di Indonesia. Pengobatan kanker serviks seperti operasi, kemoterapi, dan radioterapi masih terus diupayakan, namun tingkat kesembuhan penderita kanker serviks masih rendah, yaitu dengan 1-year survival rate hanya sekitar 10-20%. Salah satu tanaman terapeutik di Indonesia yang memiliki aktivitas antikanker adalah bajakah tampala (Spatholobus littoralis Hassk). Nanopartikel bajakah tampala dilaporkan memiliki sitotoksisitas sedang terhadap sel HeLa. Namun, belum diketahui konsentrasi optimal ekstrak bajakah tampala terhadap sel HeLa berdasarkan viabilitas dan ultrastruktur sel. Tujuan dari penelitian ini adalah mengetahui pengaruh variasi konsentrasi ekstrak bajakah tampala (100, 150, 200 dan 250 µg/mL) terhadap viabilitas sel HeLa menggunakan trypan blue dan ultrastruktur sel HeLa dengan scanning electron microscope (SEM). Hasil analisis statistik dengan tingkat kepercayaan 0,05 menunjukkan terdapat perbedaan signifikan nilai persentase viabilitas sel antara kontrol dan perlakuan konsentrasi ekstrak bajakah tampala. Hasil pengamatan ultrastruktur dengan scanning electron microscope menunjukkan terdapat perbedaan ultrastruktur sel HeLa antara sampel kontrol dengan perlakuan. Hasil tersebut menunjukkan bahwa konsentrasi ekstrak bajakah tampala 100 μg/mL, 150 μg/mL, 200 μg/mL dan 250 μg/mL memiliki pengaruh terhadap viabilitas dan ultrastruktur sel HeLa. Konsentrasi 200 μg/mL cenderung lebih mampu menekan viabilitas sel dan menunjukkan karakteristik apoptosis secara ultrastruktur. ...... Cervical cancer is one of the cancer that most women suffer from in Indonesia. Treatment for cervical cancer such as surgery, chemotherapy and radiotherapy is still being attempted, but the cure rate for cervical cancer sufferers is still low, with a 1-year survival rate of only around 10-20%. One of the therapeutic plants in Indonesia that has anticancer activity is bajakah tampala (Spatholobus littoralis Hassk.). Bajakah tampala nanoparticles were reported to have moderate cytotoxicity against HeLa cells. However, the optimal concentration of bajakah tampala extract for HeLa cells is not yet known based on cell viability and ultrastructure. The aim of this research was to determine the effect of varying concentrations of bajakah tampala extract (100, 150, 200 and 250 µg/mL) on HeLa cell viability using trypan blue and HeLa cell ultrastructure using a scanning electron microscope (SEM). The results of statistical analysis with a confidence level of 0.05 show that there is a significant difference in the percentage value of cell viability between the control and the bajakah tampala extract concentration treatment. The results of ultrastructural observations using a scanning electron microscope showed that there were differences in the ultrastructure of HeLa cells between control and treated samples. These results indicate that the concentrations of bajakah tampala extract of 100 μg/mL, 150 μg/mL, 200 μg/mL and 250 μg/mL have an influence on the viability and ultrastructure of HeLa cells. A concentration of 200 μg/mL tends to be more capable of suppressing cell viability and shows ultrastructural characteristics of apoptosis.

Abstrak :
Penelitian terfokus pada Formasi Klapanunggal pada kala Miosen Tengah tersusun atas batugamping yang merupakan bagian dari Cekungan Jawa Barat dan Palung Bogor bagian Utara. Tujuan dari penelitian adalah menentukan karakteristik fasies, lingkungan pengendapan, dan sejarah diagenesis batugamping pada Formasi Klapanunggal, Jawa Barat. Metode penelitian yang dilakukan adalah pengumpulan data dan pengukuran penampang stratigrafi, analisis petrografi, X-ray Diffraction, dan Scanning Electron Microscope. Klasifikasi fasies yang didapatkan dari hasil deskripsi makroskopis dan analisis petrografi sebanyak enam (6) fasies, yaitu Skeletal Wackestone, Skeletal Packstone, Benthic Foram Grainstone, Skeletal Rudstone, Coral Rudstone, dan Bindstone. Kemudian, fasies-fasies tersebut diklasifikasikan menjadi asosiasi fasies berdasarkan makroskopis dan petrografi menghasilkan tiga (3) asosiasi fasies, yaitu Platform Interior (Open Marine), Platform Margin Reef, dan Slope. Selanjutnya, hasil analisis diagenesis dan paragenetik menghasilkan sejarah diagenesis batugamping pada Formasi Klapanunggal secara berurutan, yaitu tahapan eogenetik (marine phreatic dan meteoric phreatic), tahapan mesogenetik (burial), dan tahapan telogenetik (meteoric phreatic dan meteoric vadose). Kemudian, terdapat hubungan asosiasi fasies dengan diagenesis, yaitu fitur sementasi marine lebih intensif pada asosiasi fasies Platform Margin Reef dibandingkan dengan asosiasi fasies Slope dan Platform Interior (Open Marine). Selain itu, pengaruh kompaksi mekanik lebih kuat pada asosiasi fasies Platform Margin Reef dan Slope dibandingkan dengan asosiasi fasies Platform Interior (Open Marine). ......The research focused on the Klapanunggal Formation during the Middle Miocene which was composed of limestone which was part of the West Java Basin and the Northern part of the Bogor Trench. The purpose of this research is to determine facies characteristics, facies association, depositional environment and the history of limestone diagenesis in Klapanunggal Formation, West Java. Research method is data collection and measurement of stratigraphic sections, petrographic analysis, X-ray Diffraction, and Scanning Electron Microscope. The classification of facies obtained from the result of macroscopic description and petrographic analysis as many as six (6) facies, namely Skeletal Wackestone, Skeletal Packstone, Benthic Foram Grainstone, Skeletal Rudstone, Coral Rudstone, and Bindstone. Then, those facies classified into facies association based on macroscopic and petrographic analysis produces in three (3) facies association, namely Platform Interior – Open Marine, Platform Margin Reef, and Slope Furthermore, the results of diagenesis and paragenetic analysis produce a history of limestone diagenesis in the Klapanunggal Formation sequentially, namely the eogenetic stages (marine phreatic and meteoric phreatic), mesogenetic (burial) stages, and telogenetic stages (meteoric phreatic and meteoric vadose). Then, there is a relationship between facies associations with diagenesis, namely marine cementation features are more intensive in the Platform Margin Reef facies association than the Slope and Platform Interior (Open Marine) facies associations. In addition, the effect of mechanical compaction is stronger on the Platform Margin Reef and Slope facies association compared to the Platform Interior (Open Marine) facies association.

Abstrak :
Material nilon memiliki banyak kegunaan dan aplikasi yang luas di berbagai bidang. nilon terkenal dengan higroskopisitasnya yakni menyerap kelembaban dari lingkungan sekitar dan juga rentan terhadap degradasi dengan adanya uap air pada temperatur tinggi. Penggunaan nilon dalam jangka waktu yang cukup panjang, perlu pertimbangan yang lebih lanjut mengenai kekuatan serta umur pakainya. Pelapis silika merupakan produk lapisan nano yang terdiri atas oksida dan silikon. Dengan dimensi yang kurang dari 100 nm dan disebut sebagai nanopartikel ini menawarkan berbagai keuntungan dalam memproteksi material seperti hidrofobisitas, stabilitas pH, dan konsistensi dimensi. Tujuan dari penelitian ini adalah untuk menganalisis pengaruh pelapis silika pada nilon hasil cetak tiga dimensi (3D) terhadap daya serap air dan uap air pada temperatur tinggi. Penelitian ini dilakukan dengan beberapa tahapan proses, dimulai dari pencetakan sampel dengan pencetakan 3D, pengaplikasian lapisan SiO2, perlakuan penuaan hidrotermal, analisa daya serap air dan desorpsi massa, uji tekuk, dan pengamatan melalui mikroskop elektron. Hasil penelitian menunjukan bahwa terdapat pengaruh pelapis silika yang diaplikasikan pada sampel nilon hasil cetak tiga dimensi (3D) terhadap penuaan hidrotermal, baik sebagai penghalang penyerapan kelembapan, kekuatan mekanis tambahan, dan juga pelindung terhadap pembebanan. ......Nylon material has many uses and wide applications in various fields. Nylon is famous for its hygroscopicity, namely absorbing moisture from the surrounding environment and is also susceptible to degradation in the presence of water vapor at high temperatures. When using nylon for a long period of time, further consideration is needed regarding its strength and lifespan. Silica coating is a nano-coating product consisting of oxide and silicon. With dimensions of less than 100 nm and referred to as nanoparticles, they offer various advantages in protecting materials such as hydrophobicity, pH stability and dimensional consistency. The aim of this research is to analyze the effect of silica coating on three-dimensional (3D) printed nylon on the absorption capacity of water and water vapor at high temperatures. This research was carried out in several process stages, starting from printing the sample using 3D printing, applying a silica coating, hydrothermal aging treatment, analyzing water absorption and mass desorption, bending tests, and observing via an electron microscope. The research results show that there is an effect of silica coating applied to three-dimensional (3D) printed nylon samples on hydrothermal aging, both as a barrier to moisture absorption, additional mechanical strength, and also protection against loading.

Abstrak :
Latar Belakang : Studi ini mengevaluasi sifat mekanik kekuatan tekan dan sifat fisik morfologi permukaan Resin Komposit Bulk-fill Packable dengan Flowable. Kedua material ini memiliki kemampuan berpenetrasi cahaya hingga kedalaman 4 mm namun dengan viskositas yang berbeda. Keduanya memiliki kandungan monomer dan konsentrasi filler yang berbeda dimana hal tersebut mempengaruhi sifat mekanik dan fisiknya. Tujuan : Mengetahui perbandingan kekuatan tekan Resin Komposit Bulk-fill Packable dengan Flowable, serta hubungannya dengan morfologi permukaan. Metode : 12 spesimen Resin Komposit Bulk-fill Packable dan Flowable disiapkan untuk uji kekuatan tekan lalu dianalisis dengan uji Independent T-test. Kemudian spesimen Resin Komposit Bulk-fill Packable dan Flowable disiapkan untuk analisis morfologi permukaan menggunakan SEM. Hasil Penelitian : Tidak terdapat perbedaan bermakna nilai kekuatan tekan antara Resin Komposit Bulk-fill Packable dan Flowable dengan nilai kekuatan tekan RK Bulk-fill Packable lebih tinggi dibandingkan dengan Flowable. Resin Komposit Bulk-fill Packable memiliki permukaan eksternal yang lebih tidak beraturan, ukuran partikel yang beragam, dan lebih banyak porus. Kesimpulan : Resin Komposit Bulk-fill Packable menunjukkan nilai kekuatan tekan yang sama dengan Resin Komposit Bulk-fill Flowable. Material RK Bulk-fill Packable memiliki morfologi permukaan yang tidak beraturan, lebih banyak porus, dan ukuran partikel yang lebih beragam dibandingkan dengan RK Bulk-fill Flowable. ......Background : This study evaluates the compressive strength and surface morphology of the Resin Composite Bulk-fill Packable and Flowable. Both of these materials have the ability to penetrate light to a depth of 4 mm but with different viscosities. Each Resin Composite Bulk-fill have different monomer content and filler concentrations which affect their mechanical and physical properties. Objective : Knowing the comparison of the compressive strength of Resin Composite Bulk-fill Packable with Flowable, and its relationship with surface morphology. Methods : 12 specimens of Resin Composite Bulk-fill Packable and Flowable were prepared for compressive strength test and then analyzed by Independent T-test. Then specimens of Resin Composite Bulk-fill Packable and Flowable were prepared for surface morphology analysis using SEM. Results : There was no significant difference in the compressive strength values between Resin Composite Bulk-fill Packable and Flowable with the compressive strength of Resin Composite Bulk-fill Packable being higher than Flowable. Resin Composite Bulk-fill Packable have a more irregular external surface, a variety of particle sizes, and are more porous. Conclusion : Resin Composite Bulk-fill Packable shows the same compressive strength value as Resin Composite Bulk-fill Flowable. The Resin Composite Bulk-fill Packable material has an irregular surface morphology, more voids, and more diverse particle size compared to Resin Composite Bulk-fill Flowable.