Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Gejala infeksi virus chikungunya dapat menjadi hambatan sosioekonomi sehingga diperlukan deteksi dini infeksi tersebut. Badan Pengkajian dan Penerapan Teknologi (BPPT) memulai pengembangan kit diagnostik infeksi virus chikungunya berbasis imunologi menggunakan Rapid Diagnostic Test dari antibodi monoklonal envelope 1 (E1). Penelitian kloning dan ekspresi gen E1 virus chikungunya dari isolat Jambi bertujuan untuk memperoleh plasmid rekombinan pYES2-E1 dan protein rekombinan E1 dari host Saccharomyces cerevisiae INVSc1. Teknik yang digunakan yaitu kloning DNA plasmid pembawa gen E1 yang akan ditransformasikan ke Escherichia coli dan S. cerevisiae. Analisis hasil ekpresi protein E1 rekombinan menggunakan teknik western blot. Hasil dari penelitian ini menunjukkan plasmid rekombinan pYES2-E1 diperoleh menggunakan verifikasi teknik PCR dan double digestion. Hasil ekspresi protein rekombinan E1 pada S. cerevisiae INVSc1 menunjukkan terdapat band yang berukuran 34—43 kDa menggunakan teknik western blot. Protein E1 rekombinan yang berhasil terekspresi pada S. cerevisiae diperlukan validasi menggunakan antibodi monoklonal E1. Hasil isolasi plasmid pYES2-E1 dilanjutkan dengan verifikasi sekuensing DNA.

---

Symptoms of chikungunya virus infection can be a socioeconomic challenges, so early detection of the infection is needed. The Agency for the Assessment and Application of Technology (BPPT) began the development of an immunology-based chikungunya virus infection diagnostic kit using the Rapid Diagnostic Test from monoclonal envelope 1 (E1) antibodies. Research on cloning and expression of the chikungunya virus E1 gene from the Jambi isolate aimed to obtain recombinant pYES2-E1 plasmids and E1 recombinant proteins from the host Saccharomyces cerevisiae INVSc1. The technique used is to clone plasmid DNA E1 gene carriers which will be transformed into Escherichia coli and S. cerevisiae. Analysis of recombinant E1 protein expression using the western blot technique. The results of this study indicate that the recombinant pYES2-E1 plasmid was obtained using PCR verification techniques and double digestion. The results of E1 recombinant protein expression in S. cerevisiae INVSc1 showed a band of 34—43 kDa using western blotting technique. The recombinant E1 protein in S. cerevisae that was successfully expressed required validation using monoclonal E1 antibodies. The results of the pYES2-E1 plasmid isolation were followed by verification of DNA sequencing.

Abstrak :
ABSTRAK
Penelitian Microbial Fuel Cell skala laboratorium dilaksanakan dengan tujuan untuk mengetahui kapasitas dan efisiensi produksi energi listrik dalam sistem Microbial Fuel Cell dengan menggunakan mikroorganisme. Medium yang digunakan merupakan golongan bakteri berupa isolat dari bakteri Saccharomces cereviciae. Sejumlah media dievaluasi kapasitasnya dalam memberikan fase pertumbuhan yang terbaik untuk Saccharomces cereviciae menggunakan metode Optical Density dengan Spektrofotometer pada panjang gelombang 550 nm. Proton Exchange Membrane yang digunakan adalah jenis Nafion 117, Lynctech, USA. Elektroda yang digunakan sebagai mediator elektron pada kedua kompartmen baik anoda maupun katoda, merupakan elektroda grafit di dalam bejana bervolume 5 x 10-2 m. Sedangkan pada kompartmen katoda merupakan elektrolit berupa campuran senyawa K3Fe(CN)6 dan K2HPO4. Mikroba yang telah dikultur akan diaplikasikan ke dalam reaktor Microbial Fuel Cell untuk dibaca kemampuannya dalam menghasilkan energi listrik dengan mengaplikasikannya pada sistem elektrik yaitu sebuah digital multimeter (microampermeter) dengan penghubung kabel sepanjang 3,0 x 10-1 m. Elektron dialirkan melalui sebuah grafit seluas 1.46 x 10-3 m2 untuk diukur besar kuat arus dan tegangannya. Sejumlah faktor perlu dikontrol sehingsga mikroba dapat menghasilkan energi listrik secara efisien, diantaranya dengan melakukan pengukuran terhadap derajat keasaman dan nilai DO dalam kompartemen anoda. Dari hasil penelitian MFC, diperoleh efisiensi listrik sebesar 53,90% untuk perbandingan antara meggunakan dan tanpa riboflavin sebagai mediator. Sedangkan penambahan minyak nabati ke dalam sistem MFC menghasilkan nilai optimum sebesar 189 µA. Selain itu, dalam penelitian ini diperoleh bahwa minyak nabati yang ditambahkan saat inokulasi Saccharomyces cerevisiae, terbukti dapat meningkatkan kadar riboflavin hingga 42,19 % selama 35 jam proses inkubasi.

---

ABSTRACT
A laboratory-scale of Microbial Fuel Cell carried out in order to determine the capacity and efficiency of electricity production in microbial fuel cell systems by using microorganisms. The medium used is an isolate culture of Saccharomces cereviciae. A number of media evaluated its capacity to provide the best growth phase for Saccharomces cereviciae using Optical Density method with spectrophotometer at a wavelength of 550 nm. Proton Exchange Membrane used was kind of Nafion 117, Lynctech, USA. Electrodes are used as electron mediator in both anode and cathode compartment either, a graphite electrode in the vessel volume of 5 x 10-2 m3. While in the cathode compartment is a mixture of electrolyte compounds K3Fe(CN)6 and a buffer solution. Microbes that have been cultured to be applied into the reactor Microbial Fuel Cell for reading ability in generating electrical energy by applying it to the electric system is a digital multimeter (microampermeter) with connecting cable along the 3.0 x 10-1 m. Electrons flow through a graphite covering 1,46 x 10-3 m2 to measure the large currents and voltage. A number of factors need to be controlled so that microbes can generate electrical energy efficiently, such as by measuring the degree of acidity and the DO in the anode compartment. From the results of MFC research, obtained by electrical efficiency of 53.90% for the comparison between receipts and without riboflavin as a mediator. While the addition of vegetable oil into the MFC system produces the optimum value of 189 μA. In addition, in this study shows that vegetable oils are added during inoculation of Saccharomyces cerevisiae, is proven to increase levels of riboflavin up to 42.19% after 35 hours incubation process.

Abstrak :
The bioetanol development from biomass bases of lignocellulose like bagasse is one of alternative energy which has potential to be applied in Indonesia. Beside of raw material source that is so many in our country, the process is also environmentally friendly. Conversion of bagasse becomes etanol using Simultaneous Sacharification and Fermentation (SSH technology by cellulose and cellobiase enzyme had been done on this research. Sacharification process or hydrolysis process, cellulose enzyme will break cellulose polymer becomes glucose whereas cellobiose enzyme will break cellobiose becomes glucose. Then, glucose through fermentation is changed to etanol by using yeast Saccharomyces cerevisiae. The variations include pH of system that is pH 4' ; 4,5 and 5, HCI addition low concentrated HCI at pH 5 with variation of concentration that is 0,5 % and I %, also variation of sample at pH 5 where bagasse without pretreatment is compared with bagasse which had been done pretreatment by using fungi Lentinus edodes for 4 weeks. The result shows that the use of cellulose and cellobiase enzyme with system optimum condition pH 5 produce etanol concentration is higher than using only cellulose enzyme at the same pH condition. For substrate concentration 50 g/L, on the use of cellulose and cellobiase, the highest etanol concentration which is produced bagasse without pretreatment is 5,62 g/L or li,24 % from bagasse. On HCI addition, the highest etanol concentration is produced by concentration HCI i % with amount 6,52 g/L or 13,04 % from bagasse. With bagasse L. edodes and P. ostreatus 6 weelts, the highest etanol concentration that is 6 86 g/L and 6,50 g/L or 13, 72% and l2,99% from bagasse. It also shows that HCl addition low concentrated and pretreatment by white rot fungi L. edodes and P. ostreatus can increase the etanol quantity that is produced from bagasse conversion.