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Abstrak :
Objective: To investigate the phenotype and genotype of E. faecalis isolated from the root canal and saliva of primary endodontic patients with periapical lesions. Methods: Eighte variations en adult male and female individuals suffering from primary endodontic infection, either with or without periapical lesions, were involved in this study. Root canal scraping and saliva were collected from each subject and used for bacterial quantitation using a real-time polymerase chain reaction (RT-PCR). Enterococci were isolated using ChromAgar medium and then identified using both biochemical (Gram staining and catalase tests) and molecular biology (conventional PCR) methods. Gelatinase activity, polysaccharide capsul profile and mRNA ace expression level were determined using microbiological, biochemical and molecular biology approach, respectively. Genotype of E. faecalis was determined based on nucleotide sequence of ace and gelE genes analyzed using web-based 3730xl DNA Analyze software. Results: The results showed that except for its proportion, no significant difference was found in phenotypes (gelatinase activity and mRNA ace expression levels) and genotypes (polymorphism of Cps operon and variation of ace and gelE nucleotide sequences) of E. faecalis isolated from the root canal and saliva of primary endodontic patients had or had no periapical lesions. Conclusion: It can be concluded that E. faecalis proportion had a role in the occurrence of periapical lesions in the primary endodontic patients, but not gelatinase activity, mRNA ace expression level, Cps operon polymorphism or ace and gelE nucleotide sequence variations.

Abstrak :
Abfraction, a cervical non caries lesion which occur mostly as a result of traumatic occlusion, needs to be taken into consideration. The aim of this case study is to understand how to treat abfraction lesion. First the focus of treatment is only to endodontic case but pain was still existed after the root canal treatment and it revealed that there were abfraction lesion on the other 5 teeths. Treatment of those lesions consisted of filling with GIC cement and occlusal adjustment.