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"Fatmawati U, Suranto, Sajidan. 2009. Protein expression on Cr resistant microorganism using electrophoresis method. Nusantara Bioscience 1: 31-37. Hexavalent chromium (Cr(VI)) is known as toxic heavy metals, so the need is reduced to Cr(III) is much less toxicity. Pseudomonas aeruginosa, Pseudomonas putida, Klebsiella pneumoniae, Pantoea sp. and Saccharomyces cerevisiae are resistant Cr(VI) microorganism and have ability to reduce Cr(VI). The aim of this research is to know ability of microorganism to reduce Cr(VI) and to know protein band pattern between Cr(VI) resistant microorganism and non resistant microorganism which inoculated on LB broth. SDS-PAGE was used to indentify protein expression. While, Cr(VI) concentration was identified by 1.5 diphenylcarbazide method. The quantitative data was analyzed by two factorial ANOVA that continued with DMRT at 1% level test. The qualitative data i.e. protein expression analyzed by relative mobility (Rf). The results showed that the ability of microorganisms to reduce Cr(VI) at initial concentration of 0.5 ppm, 1 ppm, 5 ppm and 10 ppm may vary, the average percentage of the ability of each microorganism in reducing Cr(VI) is P. putida (65%) > S. cerevisiae (64.45%) >. P. aeruginosa (60.73%) > Pantoea sp. (50.22%) > K. pneumoniae (47.82%) > without microorganisms (34.25%). The adding microorganisms have significantly influenced toward reduction of Cr(VI). The SDS-PAGE shows that protein expression between resistant and not resistant microorganisms are no different, but resistant microorganisms have more protein (protein band is thicker)."

"Kurkumin, bahan aktif tanaman kurkuma diduga bermanfaat dalam pengobatan penyakit hati. Dalam penelitian terdahulu, diperlihatkan efek protektif kurkumin terhadap peroksidasi lipid dan swelling mitokondria yang diisolasi dari hati tikus karena pemberian t-butilhidroperoksida (t-BuOOH). Dalam penelitian ini, pemberian t-BuOOH 90 M menyebabkan mitokondria tidak dapat membentuk potensial transmembran (m). Kegagalan pembentukan potential transmembran diduga berhubungan dengan transisi permeabilitas dan apoptosis. Dari 3 dosis kurkumin yang dicoba (0,5 M, 2,5 M dan 5,0 M), ternyata kurkumin dosis 2,5 M dapat mencegah kegagalan pembentukan potensial transmembran akibat t-BuOOH (79,13 + 6,28%). Pemeriksaan elektroforesis protein mitokondria menunjukkan kurkumin 1000 M dapat mencegah agregasi protein yang terjadi akibat t-BuOOH. Dari penelitian ini diperlihatkan efek proteksi kurkumin terhadap kerusakan sistem pembentukan energi dan protein mitokondria yang disebabkan oleh t-BuOOH. (Med J Indones 2007; 16:139-45)

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Curcumin, an active ingredient of curcuma plant has been thought to be beneficial in the treatment of liver diseases. In the previous studies, we have shown the protective effects of curcumin against lipid peroxidation and swelling of the rat liver mitochondrial preparation induced by tert-butylhydroperoxide (t-BuOOH). In the present study, the administration of t-BuOOH of 90 M caused the mitochondria failed to generate a transmembrane potential (m). Of 3 doses of curcumin administered (0.5 M, 2.5 M dan 5.0 M) the maximum protective effect against failure to generate a transmembrane potential caused by t- BuOOH was obtained by 2.5 M of curcumin (79.13 + 6.28%). Further, curcumin of 1000 M could prevent protein aggregation formation caused by t-BuOOH in the electrophoretogram. This study shows the protective effects of curcumin against damaged of energy production system and protein of the mitochondria caused by t-BuOOH. (Med J Indones 2007; 16:139-45"

"Amyloidosis is a rare disease, when diagnosed it's incurable and mostly affect over 40 years old male. Diagnostic is confirmed if histopathologic stained positive with Congo red and evidence of monoclonal protein. Survivals for untreated patients are 13 months in primary amyloidosis but if secondary to other chronic disease and systemic, survival could be 3-4 years. It can not be prevented but when affected, control of the underlying illness can prevent progression of amyloidosis.

We report a rare case of a 67 year old male, who came with chronic diarrhea. The stool analysis, there were no negative gram microorganisme found, only food maldigestion and fungus infection. Stool analyze from parasitology department were found microspore, but the stool culture were sterile. The patient underwent colonoscopy which revealed hyperemis mucosa in rectum, sigmoid, descending & transverse colon. From the biopsy was concluded intestinal amyloidosis. We treated the patient symptomatically and couldn't find the underlying inflammatory disease which causes the problem."