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"Teh hijau diolah dari daun teh yang tidak difermentasi. Berbagai aktivitas biologis teh hijau telah dilaporkan. Bentuk infus dan kandungan polifenolnya telah diketahui mempunyai efek antimutagenik, antibakterial, menurunkan kadar kolesterol, antioksidan, dan mitogen limfosit B. Penelitian membuktikan bahwa polifenol teh hijau dapat meningkatkan produksi IL-12. Infeksi yang disebabkan oleh kuman salmonella spp sampai saat ini masih merupakan masalah kesehatan di berbagai negara di seluruh dunia. Peran imunitas tubuh, diantaranya imunitas seluler yang diperantarai sel T helper sangat diperlukan untuk mengatasi infeksi akibat kuman ini. Berbagai penelitian menunjukkan bahwa IL-12 berperan penting dalam mekanisme imunitas seluler. Penelitian ini bertujuan untuk mengetahui efek polifenol terhadap respon imun seluler mencit selama infeksi Salmonella typhimurium. Subjek penelitian adalah mencit Balb/C betina berumur 6-8 minggu yang dibagi menjadi 3 kelompok. Kelompok pertama mendapat polifenol dosis 10 mg/hari, kelompok kedua 5 mg/hari selama 1 bulan, dan kelompok ketiga tidak mendapat polifenol. Pada hari ke-31 semua kelompok diinfeksi dengan Salmonella typhimurium 108 CFU per oral. Pada hari 0, 3, 5, dan 7 setelah infeksi masing-masing kelompok dibunuh 3 ekor mencit untuk diekstraksi splenosit dan sel makrofag peritonealnya. Kadar IFN-g supernatan kultur splenosit dan aktivitas fagosit oleh makrofag peritoneum diperiksa pada hari tersebut. Kadar IFN-g pada supernatan kultur splenosit meningkat selama infeksi pada semua kelompok, tetapi kadarnya pada kelompok yang mendapat polifenol lebih tinggi daripada kelompok kontrol. Persentase aktivitas fagositosis makrofag peritoneal juga lebih tinggi pada kelompok yang mendapat polifenol daripada kelompok kontrol. Peningkatan aktivitas fagositosis makrofag ini berkorelasi positif dengan kadar IFN-g pada supernatan kultur splenosit. (Med J Indones 2004; 13: 1-7)

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Green tea is an aqueous infusion of dried unfermented leaves of Camellia sinensis. Numerous biological activities of green tea have been reported. The aqueous infusion and its polyphenolic substance are known for their activity as an antimutagenic, antibacterial, hypocholesterolemic, antioxidant, and mutagenic of B lymphocyte. Studies have demonstrated that green tea polyphenols increase IL-12 production. Salmonella spp infection is an important public health problem in many countries. Cell-mediated immunity (CMI), especially T-cell help is important for protection against this infection. Recent evidence indicates that IL-12 is one such factor that plays a crucial role in the development of CMI. These studies were carired out to investigate the effect of green tea polyphenols to the immune cellulare in mice responses of mice during Salmonella typhimurium infection. The subject consisted of 36 female mice (Balb/C), 6-8 weeks old, divided into 3 groups. The first group was given 10 mg polyphenols/mouse, the second group was given 5 mg polyphenols/mouse, and the third group as the control. In day 31, all mice were infected with 108 CFU Salmonella typhimurium orally. On day 0, 3, 5, and 7 postinfection, 3 mice from each groups were sacrificed, the splenocytes were extracted and cultured to measure the level of IFN-g in the supernatan and. The peritoneal macrophages were also extracted and cultured to measure the phagocytic activity. The level of IFN-g in splenocyte culture supernatant increased during infection in all groups, but the level of the experimental groups were higher than in control group. The percentage of phagocytic activity of peritoneal macrophages were higher in the experimental groups than in the control group. The increase of the phagocytic activities were seen corelate with the level of IFN-g supernatan splenocyte culture. (Med J Indones 2004; 13: 1-7)"

"Tuberkulosis (TB) sampai dengan saat ini masih merupakan salah satu masalah kesehatanutama di Indonesia. Sepuluh persen dari TB ekstraparu adalah TB tulang, dan sekitar 50%penderita TB tulang menyerang tulang belakang (Spinal Tuberkulosis). Respon tubuhterhadap Mycobacterium tuberculosis (M.tb) sehingga menimbulkan penyebaranekstraparu, khususnya respon makrofag sebagai pertahanan lini pertama, masih belumsepenuhnya dimengerti. Makrofag menghasilkan molekul reactive oxygen species (ROS)sebagai hasil dari oxygen burst untuk mengeliminasi antigen. Nitrat Oksida (NO) danmieloperoksidase (MPO) berperan pada oxygen burst Selain itu, Pada fagositosisterdapat organel lisosom yang di dalamnya terdapat enzim hidrolase (fosfatase asam danbeta glukuronidase) berguna pada pencernaan intraseluler. Penelitian ini mengujihipotesis bahwa ada gangguan fungsi makrofag pada pasien TB tulang belakang. Monositdiisolasi dari peripheral blood mononuclear cells (PBMC) dari lima pasien TB tulangbelakang dan lima orang sehat sebagai kontrol. Monosit yang terisolasi dikultur denganstimulasi dari macrophage colony-stimulating factor (M-CSF) selama tujuh hari untukpematangan. Kemampuan fagositosis makrofag dinilai aktivitasnya terhadapa sel darahmerah domba (SDMD). Sedangkan nitrat oksida (NO), mieloperoksidase (MPO), betaglukuronidase,dan aktivitas fosfatase asam diselidiki dengan metode spektrofotometer.Analisis data dengan menggunakan aplikasi SPSS versi 20. Kami menemukan bahwamonosit yang diisolasi dari PBMC pasien TB tulang belakang secara signifikan lebihsedikit dibandingkan dengan kelompok kontrol (2992.103 vs 6474.103 (sel / mL)) danjuga lebih sedikit makrofag yang melekat pada sel darah merah domba (SDMD) (264.103vs 598.103 (sel / mL)). Namun, produksi NO (2346 vs 325,17 (μmol / gram protein)), danMPO (570,7 vs 17,4 (unit / mg), beta-glukuronide (0,149 vs 0,123 (unit / mg)), dan asamfosfatase aktivitas (1776,9 vs 287,9 (unit / mg)) dari makrofag kelompok TB tulangbelakang lebih tinggi daripada kelompok yang sehat serta korelasi negatif kuat danbermakna antara fagositosis makrofag dengan tiap variabel tersebut. Selain itu, Terdapatkorelasi positif lemah dan tidak bermakna antara kejadian fagositosis dan uji WST.Meskipun pengenalan rendah pada benda asing, proses makrofag intraseluler, termasukaktivitas oksidatif dan fungsi lisosom, tinggi secara signifikan. Hasil ini menunjukanpenurunan fungsi makrofag pada pasien TB tulang belakang serta terdapat kemungkinanadanya dominasi imunitas non-spesifik bawaan pada infeksi TB tulang belakangTuberculosis (TB) is still one of the main health problems in Indonesia. Ten percent ofextrapulmonary TB is bone TB and about 50% of people with bone TB affected to thespine. The immune response against Mycobacterium tuberculosis (M.tb), which causesextrapulmonary spread, particularly the response of macrophages as a first-line defense,is still not fully understood. Macrophages produce reactive oxygen species (ROS)molecules as a result of oxygen bursts to eliminate antigens. Nitric Oxide (NO) andmyeloperoxidase (MPO) play a role in oxygen burst. Also, phagocytosis process involvedlysosomal organelles in which there are hydrolase enzymes (acid phosphatase and betaglucuronidase),which have important role in intracellular digestion. This study examinedthe hypothesis about impairment of macrophage function in spondylitis TB patients.Monocytes were isolated from peripheral blood mononuclear cells (PBMC) collectedfrom five spinal TB patients and five healthy people as controls. Isolated monocytes werecultured by stimulation of macrophage colony-stimulating factor (M-CSF) for seven daysfor maturation. The phagocytic ability of macrophages is assessed as to their activity onsheep red blood cells. Whereas nitric oxide (NO), myeloperoxidase (MPO), betaglucuronidase,and acid phosphatase activity were investigated by spectrophotometermethods. Data was analyzed using SPSS version 20. We found that monocytes isolatedfrom PBMC of spondylitis TB patients were significantly less than in the control group(2992,103 vs 6474,103 (cells / mL)) and also fewer macrophages attached to red bloodcells sheep (264,103 vs 598,103 (cells / mL)). However, NO production (2346 vs 325.17(μmol / gram protein)), MPO (570.7 vs. 17.4 (units/mg), beta-glucuronide (0.149 vs 0.123(units/mg)), and acids phosphatase activity (1776.9 vs 287.9 (units/mg)) of macrophagesin the spondylitis TB group were higher than in the healthy group. There was a strongand significant negative correlation between phagocytosis of macrophages with each ofthe previous variables. There was no significant difference between phagocytic abilityand the WST test. Although the recognition against foreign bodies was low, intracellularmacrophage processes, including oxidative activity and lysosomal function, weresignificantly higher than control. This result showed a decrease of macrophage functionin spondylitis TB patients as well as a possible dominance of non- specific immunity."

"Pendahuluan: Interaksi Cryptococcus neoformans dengan makrofag mempengaruhi kejadian kriptokokosis meningeal, infeksi oportunistik fatal pada populasi AIDS, dimana pada keadaan imunokompromi, kemampuan fagositosis makrofag terganggu. Penelitian ini bertujuan untuk menganalisis aktifitas fagositik makrofag penderita HIV, profil sitokin yang terbentuk serta pengaruh pemberian obat anti retroviral. Metode: Desain penelitian eksploratif-analitik terhadap interaksi makrofag-C. neoformans. Makrofag pasien HIV dan orang sehat (selanjutnya disebut Kasus dan Kontrol). Penelitian mencakup pengukuran kadar nitrit petanda aktivasi makrofag, uji indeks internalisasi jamur (IIJ), laju fagositosis (LF), dan daya bunuh (DB) makrofag terhadap jamur yang diamati pada menit ke 30, 120 dan 240. Selain itu juga diteliti profil sitokin yang terbentuk (IL-5, IL-10, IL-6, TNF-?, IFN-?) dan uji serologis terhadap plasma menggunakan Cryptococcus antigen lateral flow assay (CrAg-LFA). Hasil: Terkumpul 38 Kasus dan 42 Kontrol dengan hasil uji LFA seluruh subyek, Kasus maupun Kontrol, negatif. Kadar nitrit yang terbentuk lebih tinggi pada kelompok Kontrol. IIJ makrofag Kasus lebih tinggi pada T30 dan T120. LF makrofag kontrol lebih tinggi pada T30 dan T120. DB makrofag Kontrol jauh lebih tinggi dibanding makrofag Kasus pada seluruh pengamatan. Pola sitokin yang terbentuk oleh makrofag kasus mengarah ke sitokin anti inflamasi (IL-5 dan IL-10 tinggi), sedangkan pola sitokin yng terbentuk oleh makrofag Kontrol mengarah ke sitokin pro inflamasi (IL-6 dan IFN-? tinggi) kecuali untuk TNF-? yang lebih tinggi pada supernatan makrofag Kasus. Pembahasan: Aktifitas fagositik makrofag Kasus terganggu, ditandai dengan daya bunuh yang jauh lebih rendah. Selain itu, tingginya kadar sitokin pro inflamasi pada populasi kontrol menunjukkan pembersihan jamur yang lebih efektif sedangkan sitokin anti-inflamasi yang lebih tinggi pada subjek terinfeksi HIV memungkinkan terjadinya parasitisme intraseluler makrofag oleh C. neoformans. Kesimpulan: terdapat perbedaan daya bunuh dan pola sitokin pro dan anti inflamasi pada subjek terinfeksi HIV dibanding kontrol.

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Introduction: interaction of Cryptococcus neoformans-macrophage affecting the incidence of cryptococcal meningitis, a fatal opportunistic infection in AIDS population. In immunocompromised condition, macrophage phagocytic activity was impaired. This study aimed to analyze phagocytic activity of macrophage derived from HIV infected individuals against C. neoformans, the cytokine profile and the role of antiretroviral therapy in that interaction. Method: using explorative-analytical design on the interaction between macrophageyeast seen as: internalization index, phagocytic rate, killing ability, production of cytokine and NO. We also tested the plasma against Cryptococcus antigen lateral flow assay (CrAg-LFA). Result: out of 38 HIV(+) subjects and 42 healthy subject all were negatif for LFA. Nitrite formed were higher in the Control group. Internalization index were higher in the Cases group, Phagocytosis rate were higher in the Control group: Killing ability were far superior in the Control group. Cytokine profile of the Cases group were anti inflammatory (higher IL-5 and IL-10) while in the Control group, were more pro inflammatory (higher IL-6 and IFN-?) with the exception of TNF-? which was higher in the Cases group. Discussion: the higher level of pro-inflammatory cytokine among control group represent a more effective clearance of fungal by macrophages while higher level of anti-inflamatory cytokine among HIV+vderived macrophage indicates profound intracellular parasitism of macrophage by C. neoformans. Conclusion: there is difference of killing ability, NO production and antiinflammatory cytokine production among macrophage derived from healthy subjects that showed us a more effective fungal clearance and activation of macrophage."

"ABSTRAKRuang Lingkup dan Cara Penelitian: Streptokok hemolitik beta grup A (SH-A) adalah kuman patogen pada manusia menyebabkan radang tenggorok dan kulit dengan sequelae demam rematik. SH-A mempunyai protein M pada dinding selnya yang menyebabkan kuman tersebut tahan terhadap fagositosis. Tujuan penelitian ini adalah untuk mengetahui pengaruh ampisilin subkadar hambat minimal (sub KHM) terhadap daya fagositosis makrofag. Kuman SH-A dicampur dengan ampisilin sub KHM (1/4 KHM dan 1/8 KHM) dengan makrofag dan diinkubasi selama 60 menit dan 120 menit. Penelitian ini menggunakan SH-A strain standar WHO (Ceko), dan ampisilin trihidrat diperoleh dari PT Kalbe Farma. Makrofag diambil dari peritoneal mencit strain CBR umur 4-8 minggu. Sebagai kontrol dilakukan terhadap kuman yang dibiakkan dalam kaldu Todd Hewitt yang mengandung ampisilin sub KHM tanpa dicampur makrofag.Hasil dan Kesimpulan: Terdapat penurunan populasi kuman pada perbenihan yang mengandung makrofag tanpa ampisilin setelah diinkubasi 120 menit karena penurunan pH pada media. Populasi kuman menurun setelah kuman dicampur ampisilin sub KHM pada inkubasi 60 menit dan 120 menit dibandingkan dengan kontrol. Prosentase fagositosis makrofag dan indeks fagositosis makrofag terhadap kuman yang dicampur ampisilin sub KHM pada inkubasi 60 menit dan 120 menit meningkat. Dengan demikian dapat diambil kesimpulan bahwa secara in vitro daya fagositosis makrofag meningkat setelah dicampur ampisilin sub KHM pada inkubasi 60 menit dan 120 menit.

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ABSTRACT

Effect Of Ampicillin At Sub Mic On The Phagocytosis By Macrophage Of Streptococcus Hemolytic Beta Group AScope and Method of Study: Streptococcus beta-hemolyticus group A (SH-A) is pathogenic for man, the most usual causative agent for acute streptococcal upper respiratory tract and skin diseases with sequelae namely rheumatic fever. The bacterial cell wall contains protein M, a virulence factor, which is responsible for the resistance to phagocytic activity of macrophage. The aim of this research was study the phagocytosis of streptococci grown in subminimum inhibitory concentration (sub MIC) of ampicillin by macrophage after incubation for 60 and 120 minutes. SH-A was obtained from Ceko Colaboratorium (standard strain of WHO), and ampicillin trihydrate was from Kalbe Farma. The mice were kindly supplied by Central Biomedical Research, Jakarta; age 4-8 weeks, were free from infections, and used as macrophage source.

Findings and Conclusions: The number of bacteria in the medium containing macrophage after incubation for 60 minutes increase, but after 120 minutes decreases, probably due to the low pH medium. The population of bacteria decreases in the medium treated with sub MIC of ampicillin after incubation for 60 and 120 minutes. Percentage of relative effect of phagocytosis and phagocytosis index of macrophage seem to be increasing after incubation of the whole component for 60 and 120 minutes. SH-A treated with sub MIC of ampicillin underwent rapid ingestion by macrophage after incubation for 60 and 120 minutes. The result showed that the hypothesis of the rapid ingestion of SH-A treated with sub MIC ampicilin by macrophage after incubation for 60 and 120 minutes could be accepted."

"This studies was carried out to evaluate the influence of ethanol extract of phyllanthus niruri(EEPN) to the macrophage phagocytosis activity of male Swiss mice during P. berghei infection. ...."

"The aim of this study was to investigate the increase in neutrophil count and the decrease in both phagocytosis and neutrophil oxidative burst (formation of radical oxygen) among type 2 diabetes mellitus (DM) patients and the normal range of phagocytosis function and oxidative burst in neutrophil from Non-DM control subjects. The aim of this study is also to investigate the factors that influence neutrophil count, phagocytosis function and neutrophil oxidative burst among type 2 diabetes mellitus (DM) patients. The examination was conducted using a flow cytometry. The study subjects were 142 type 2 DM patients and 65 Non-DM control subjects. Statistical analysis was performed using the Mann Whitney test and linear regression analysis. The results of this study indicated that there is impaired neutrophil function among type 2 DM patients. The results of this study also showed a correlation between Hemoglobin level, age, platelet count, and SGPT vs. neutrophil phagocytosis function, as well as a correlation between HDL cholesterol and (fMLP-stimulated) neutrophil oxidative burst. The study also showed a correlation between sex and stroke and (S. sureus-stimulated) neutrophil radical oxygen formation, a correlation between neutrophil count and platelet count, a correlation offibAlc and fasting blood glucose level and (fMLP-stimulated) neutrophil radical oxygen formation. In a multivariate analysis, when adjusted to age and sex, there was a correlation between triglyceride and (baseline) neutrophil radical oxygen formation and between HDL cholesterol and (fMLP-stimulated) neutrophil radical oxygen formation."

"Pendekatan sistem imun pada pejamu M. tuberculosismerupakan salah satu pilihan dalam pengembangan terapi tuberkulosis, terutama pada kasus tuberkulosis (TB)  resisten obat. Tujuan penelitian ini adalah untuk menganalisis perbedaan fungsi makrofag pada penderita TB resisten obat dibandingkan dengan kontak erat yang terinfeksi laten dan sehat. Sel Monosit Darah Tepi (SMDT) diisolasi dan dikultur selama 7 hari. Fagositosis dinyatakan jika terdapat minimal satu sel darah merah domba tampak melekat pada membran makrofag. Kemampuan lisosom diperiksa dengan uji aktivitas enzim fosfatase asam. Enam pasien TB-RO dan 18 kasus kontak erat (8 TB laten;10 sehat) di RS Universitas Indonesia direkrut sebagai subjek penelitian. Hasil menunjukkan bahwa aktivitas fagositosis kelompok infeksi laten lebih tinggi dibandingkan kelompok sehat dan TB RO (one-way ANOVA, p<0,05). Aktivitas enzim fosfatase asam lebih tinggi pada kelompok TB RO. Perbedaan fungsi makrofag ini diharapkan dapat menjadi referensi selanjutnya dalam terapi TB RO ataupun terapi pencegahan. 

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The immune system approach to the host of M. tuberculosis is an option in developing tuberculosis therapy, especially in drug-resistant tuberculosis (DR-TB) cases. This study aimed to analyze the differences in macrophage function in drug-resistant TB patients compared to close contacts who were latently infected and healthy. Peripheral Blood Mononuclear Cell (PMBC) was isolated and cultured for seven days. Phagocytosis is expressed when at least one sheep red blood cell appears attached to the macrophage membrane. The ability of lysosomes was examined by testing the activity of the acid phosphatase enzymes. Six DR-TB patients and 18 close contact cases (8 LTBI; 10 healthy) at Universitas Indonesia Hospital were recruited as research subjects. The results showed that the phagocytosis activity of the latent infection group was higher than that of the healthy and TB RO groups (one-way ANOVA, p<0.05). Acid phosphatase activity was higher in the DR-TB group. The difference in macrophage function is expected to be a further reference in DR-TB treatment or preventive therapy."

"Air Susu Ibu tidak hanya mengandung nutrisi namun juga sel-sel imun untuk melindungi bayi dari patogen pada awal kehidupannya. Salah satu sel yang berperan penting adalah makrofag (CD14+ mononuclear cells), sebagai komponen dari sistem kekebalan bawaan bagi bayi. Penelitian ini bertujuan untuk menganalisis perbandingan populasi, viabilitas serta kemampuan fagositosis CD14+ mononuclear cells ASI dan darah tepi. Total 20 subjek dianalisis populasi CD14+ mononuclear cells, M1 (CD86) dan M2 (CD206) dengan flow cytometry. Viabilitas sel dianalisis dengan CCK assay dan kemampuan fagositosis dengan sheep red blood cell (SRBC). Hasil penelitian menunjukkan bahwa populasi CD14+ mononuclear cells ASI lebih tinggi 20% dibanding darah tepi (38,93 ± 5,29% versus 1,88 ± 0,55%, p=0.0005). Populasi CD14+ mononuclear cells ASI terbukti memiliki kemampuan polarisasi yang ditandai dengan ekspresi M1 (CD86) dan M2 (CD206). Ratio M1/M2 pada ASI adalah < 1, namun tidak memiliki perbedaan signifikan dengan darah tepi (p=0,238). Viabilitas dan kemampuan fagositosis CD14+ mononuclear cells ASI secara signifikan lebih tinggi dibandingkan darah tepi (viabilitas, p=0,0032; kemampuan fagositosis, p=0,0001). Hasil penelitian ini menunjukkan bahwa CD14+ mononuclear cells ASI mempunyai populasi yang lebih tinggi dengan polarisasi dominan M2, serta mempunyai viabilitas dan kemampuan fagosistosis yang lebih baik daripada CD14+ mononuclear cells yang berasal dari darah tepi.

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Breast milk contains nutrients and immune cells that protect infants from early-life pathogens. Macrophages (CD14+ mononuclear cells), play a crucial role as a component of the innate immune system in infants. This study compared the populations, viability, and phagocytic ability of CD14+ mononuclear cells derived from breast milk and peripheral blood in 20 subjects. The population of CD14+ mononuclear cells, M1 (CD86), and M2 (CD206) were analyzed using flow cytometry. Cell viability was assessed using the CCK assay, and phagocytic ability was measured with sheep red blood cells (SRBC). The results showed that the CD14+ mononuclear cell population in breast milk was 20% higher than in peripheral blood (38.93 ± 5.29% versus 1.88 ± 0.55%, p=0.0005. Breast milk CD14+ mononuclear cells exhibit M1 (CD86) and M2 (CD206) polarization, with an M1/M2 ratio <1, compared to peripheral blood (p=0.238). The viability and phagocytic ability of CD14+ mononuclear cells in breast milk were significantly higher compared to those in peripheral blood (viability, p=0.0032; phagocytic ability, p=0.0001). These findings indicate breast milk CD14+ mononuclear cells have a higher population with dominant M2 polarization, viability, and phagocytic ability compared to those from peripheral blood."

"Latar Belakang: Infeksi merupakan penyebab kematian yang penting pada thalassemia. Peningkatan risiko infeksi disebabkan oleh banyak faktor antara lain karena kelebihan besi dan splenektomi. Penelitian ini bertujuan mengetahui perbedaan fungsi fagositosis monosit pada pasien thalassemia mayor pasca splenektomi dan non splenektomi serta mengetahui hubungan fungsi fagositosis monosit dengan kadar feritin serum. Metode: Penelitian dilakukan di Departemen Patologi Klinik RSCM, Jakarta pada September 2013 ? Februari 2014. Desain penelitian potong lintang, dengan subjek penelitian pasien thalassemia mayor, terdiri dari 58 subjek pasca splenektomi dan 58 subjek non splenektomi yang telah dilakukan macthing umur dan jenis kelamin. Dilakukan pemeriksaan fagositosis monosit menggunakan E.coli yang telah diopsonisasi dan dilabel FITC sebagai target, (PhagotestTM) dan diperiksa dengan flow cytometry BD FACSCalibur. Kadar feritin serum diperiksa dengan Cobas e 601. Hasil: Median fagositosis monosit pada 58 subjek pasca splenektomi 5,03 (0,17 ? 22,79) %, dan pada 58 subjek non splenektomi 7,09 (0,11 ? 27,24) %, dan nilai p > 0.05. Kadar feritin serum pada subjek pasca splenektomi 6.724 (644,60 ? 21.835) ng/mL dan subjek non splenektomi 4.702,50 (1.381 ? 14.554) ng/mL, dan nilai p < 0.05. Hasil uji korelasi fungsi fagositosis monosit dengan kadar feritin didapatkan r = 0.13 (nilai p = 1.00). Kesimpulan: Tidak terdapat perbedaan bermakna antara fungsi fagositosis monosit pada pasien thalassemia mayor pasca splenektomi dan non splenektomi. Kadar feritin serum pada pasien thalassemia mayor pasca splenektomi lebih tinggi secara bermakna dibandingkan non splenektomi. Tidak didapatkan hubungan antara fagositosis monosit dengan kadar feritin serum.

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Background: Infection is an important cause of death in thalassemia. Increase of risk of infection could be due to iron overload and post-splenectomy. The study aimed to determine the difference of phagocytosis function of monocyte between post-splenectomized and non- splenectomized patients with thalassemia major, and the correlation of phagocytosis function of monocyte and serum ferritin level.

Methods: The study was conducted in Department of Clinical Pathology Cipto Mangunkusumo hospital, Jakarta, in September 2013 ? Februari 2014. It was a cross sectional study. The study subjects consisted of 58 post-splenectomized patients and 58 non-splenectomized patients with age and sex matching. Phagocytosis function of monocyte was determined using E.coli opsonized and labelled with FITC as target, (Phagotest TM) and was measured by flow cytometry BD FACSCalibur. Serum ferritin level was measured using Cobas e 601.

Result: Median phagocytosis of monocyte was 5,03 (0,17 ? 22,79) %, in 58 post- splenectomized subjects and 7,09 (0,11 ? 27,24) % in non-splenectomized subjects; p value > 0.05. Serum ferritin level was 6.274 (644,60 ? 21.835) ng/mL in post-splenectomized subjects and 4.702,50 (1.381 - 14.554) ng/mL in non-splenectomy subjects; p value < 0.05. The correlation between phagocytosis function of monocyte and serum ferritin level was r = 0.13 ( p value = 1.00).

Conclusion: There was no statistical difference of phagocytosis function of monocyte between post-splenectomized subjects and non-splenectomized subjects. Serum ferritin level in post- splenectomized was higher than non-splenectomized subjects. There was no correlation between phagocytosis function of monocyte and serum ferritin level."