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"Latar Belakang : Mycobacterium tuberculosis merupakan bakteri intraselular fakultatif penyebab Tuberkulosis (TB). Jumlah penderita 1,7 milyar orang di seluruh dunia dan terdapat penambahan 3 juta kasus baru setiap tahunnya. Prevalensi TB di Indonesia tahun 2013 sebesar 297 per 100.000 penduduk dengan kasus baru setiap tahun mencapai 460.000 kasus. Total kasus hingga 2013 mencapai sekitar 800.000-900.000 kasus. Faktor yang menghambat diagnosis TB dapat ditegakkan adalah lamanya waktu menunggu hasil kultur dan uji identifikasi penyebab TB. Menumbuhkan kuman penyebab TB berkisar 6-8 minggu. Pemeriksaan identifikasi membutuhkan waktu 3 hari sampai 1 minggu. Total waktu yang diperlukan untuk pemeriksaan kultur yaitu 7-9 minggu. Oleh karena itu dibutuhkan metode yang dapat mengidentifikasi lebih cepat dan akurat. Tujuan : Penelitian ini bertujuan mendapatkan data keberhasilan identifikasi MTB dan MOTT menggunakan alat tes berupa Kit (SD TB AgMPT64®). Mengetahui nilai sensitivitas, spesifisitas, nilai prediktif positif (NPP) dan Nilai prediktif negative (NPN) dari uji imonochromatograpic (SD TB AgMPT64®). Metode : Menggunakan uji diagnostik, baku emas yang digunakan dalam penelitian ini dengan pemeriksaan PCR TB. Sampel Penelitian ATCC Mycobaterium non tuberculosis ( MOTT ), isolate Mycobacterium tuberculosis H37RV dan isolat Mycobacterium tuberculosis yang merupakan bahan biologi tersimpan milik Departemen Mikrobiologi FKUI. Hasil : Dengan sampel 46 isolat, nilai sensitivitas dan spesifisitas ICT TB Ag MPT64 yang diperoleh 100%(IK95%: 90,4%-100%) dan 100%,(IK95%: 66,2%-100%) NPP 100% (IK95%: 90,4%-100%). NPN 100%,(IK95%: 66,2%-100%) pemeriksaan niacin dan PNB nilai sensitivitasnya 100%(IK95%: 90,4%-100%), spesifisitas 88,8%(IK95%: 51,7%-98,1%). dan NPP 97,3% (IK 95%: 86,1%-99,6%), NPN 100% (IK95%: 62,9%-100%). Kesimpulan : Analisis hasil penelitian ini menunjukkan uji identifikasi ICT TB AgMPT64 memiliki nilai sensitivitas yang sama dengan uji Niasin paper strip, uji PNB LJ dan nilai spesifisitas yang lebih tinggi.

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Background: Mycobacterium tuberculosis is a facultative intracellular bacterium causes tuberculosis (TB).The number of patients 1.7 billion people around the world and there is an addition of 3 million new cases each year. The prevalence of TB in Indonesia besad on surveillance in 2013 was to 297 per 100,000 population with new cases every year reach in 460,000 cases. Thus, the total number of cases to 2013 reached approximately 800000-900000 cases. One of the efforts to control the spread of infection is to diagnose TB quickly and accurately so it can be reach all levels of society. There are several factors that hamper the diagnosis of TB one of them is the time of culture and species identification. The identification Mycobacterium is important to determine the approproate treatment. Growing the bacteria that causes TB ranges from 6-8 weeks. Identification takes 3 days to 1 week. So the total time required for culture is 7-9 weeks. Therefore, it needs a method that can do identification more quickly and accurately.

Objective: Knowing the value of the sensitivity, specificity, positive predictive value (NPP) and negative predictive value (NPN) of an imonochromatograpic (SD TB AgMPT64®) test with the gold standard TB PCR.

Methods: This study used a diagnostic test, the gold standard used in this study was TB PCR. Sample Research ATCC mycobaterium non tuberculosis (MOTT) and isolates of Mycobacterium tuberculosis H37Rv stock, M.tuberculosis isolate which is stored biological materials belong to Department of Microbiology, Faculty of Medicine.

Results: the sensitivity and specificity of ICT TB Ag MPT64 were 100%(CI95%: 90,4%-100%) dan 100%,(CI95%: 66,2%-100%) NPP 100%(CI95%: 90,4%-100%). NPN 100%,(CI95%: 66,2%-100%) sensitivity of niacin paper strip dan PNB LJ were 100%(CI95%: 90,4%-100%), spesificity 88,8%(IK95%: 51,7%-98,1%). and NPP 97,3% (IK 95%: 86,1%-99,5%), NPN 100% (IK95%: 62,9%-100%).

Conclusion: Analysis of the results of this study indicate the identification of ICT TB test AgMPT64 have the same sensitivity as niacin paper strip test, PNB LJ and had higher specificity values."

"Riset ini merupakan pengembangan berupa pengintegrasian perangkat alat uji golongan darah tipe ABO dan Rhesus berbasis kertas menggunakan metode kromatografi dan forward grouping dengan perangkat deteksi aglutinasi secara otomatis. Kertas uji dibuat dari kertas saring Whatman nomor 4 dengan tiga kolom hidrofilik dan diletakkan pada casing berbahan Polylactic Acid hasil cetak 3D. Masing-masing kolom hidrofilik diimobilisasi reagen anti-A, anti-B, dan Anti-D (resus) 3 μl dengan 2 kali iterasi, dan 40 μl jumlah sampel darah yang dibutuhkan dalam pengujian. Perangkat deteksi aglutinasi otomatis mengimplementasikan pengolahan citra metode Absolute Substract Difference (ASD) menggunakan Raspberry Pi dan PiCamera modifikasi. Tingkat akurasi perangkat 92,5% dan waktu proses selama 4 menit, waktu proses tersebut lebih singkat dari metode point of care test golongan darah lainnya.

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This research is a development of integration for ABO and Rhesus blood group typing devices based on paper using chromatography and forward grouping method with automatic agglutination detection device. Paper test was made by Whatman No. 4 filter paper with three hydrophilic columns, and it were placed on a Polylactic Acid cases that was printed by a 3D printer. Each hydrophilic column was immobilized 3 μL of anti-A, anti-B, and Anti-D (resus) reagents with 2 fold iteration, and 40 μl of blood samples required in the test. Automatic agglutination detection device implements Absolute Substract Difference (ASD) method as a image processing using Raspberry Pi and PiCamera modification. The accuracy is 92.5% and 4 minute processing time, the processing time is shorter than the other point-of-care test blood typing methods."