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Abstrak :
ABSTRACT
Marasmiellus is a tropical and subtropical genus which consists of more than 400 species. The genus plays important roles in ecosystem as a decomposer, and not many information on its economic value. Several monographs of the genus have been published based on morphological data from several tropical forests (neotropics, Africa, Srilanka), and no report on the Indonesian Marasmiellus. At present, taxonomic problem occurs in the genus related to the position of Marasmiellus juniperinus as a type genus in the phylogenetic tree based on Internal Transcribed Spacer (ITS) region. Marasmiellus juniperinus nested into different clade from other Marasmiellus species, and it was clustered in Gymnopus clade. Since Marasmiellus has never been reported from Indonesia and the taxonomic problem occurs at the genus, thus the objectives of this study are 1) To describe the species of Marasmiellus found in Java and Bali; 2) To provide an identification key to sections and species; 3) To analyse the phylogenetic relationship within the genus Marasmiellus in Java and Bali based on morphological and molecular characters performed by Maximum Parsimony method; 4) To clarify the relationship of the genus Marasmiellus and M. juniperinus to its closely related genera with inclusion of more Marasmiellus spp. based on ITS region of rDNA sequence data; and 5) To describe novel species of Marasmiellus based on morphological and molecular data. Java and Bali were chosen as research sites in this study due to several reasons. First, historically most of Indonesian reported agarics were collected from Java, particularly from Mount Gede-Pangrango, Cibodas and Bogor Botanical Gardens. Second, forest degradation is going rapidly in Java and Bali. As a result Indonesia is loosing habitats of Marasmiellus. This study was divided into three topics according to the purposes of this study. The first topic entitled Species of Marasmiellus in Java and Bali. The study was carried out in Herbarium Bogoriense, The Botany Division, Research Center for Biology, The Indonesian Institute of Sciences (LIPI). Herbarium materials are kept in Herbarium Bogoriense (BO) and Harry D. Thiers Herbarium, San Francisco State University (SFSU), SF, CA, USA. Materials used in this research were collected in 1998-2010 from 10 different locations in Java and Bali which consisted of 22 sites. A representative material of Marasmiellus juniperinus, as a type genus, was borrowed from the Herbarium of the University of Tennessee, Knoxville, Tennessee, USA (TENN). The identification results revealed that there were 35 known species; one species as a new combination; 17 undescribed species. Those 35 species consist of 25 species of Marasmiellus found in Java, 7 species found in Bali, and 3 species found both in Java and Bali. All described species were treated based on infrageneric classification of Singer (1973) which divided the genus into 10 sections based on morphological characters. Based on Singer?s infrageneric classification Marasmiellus in Java and Bali belonged to 5 sections, i.e sect. Marasmiellus, sect. Rameales, sect. Dealbati, sect. Candidi, and sect. Stenophylloides. The second topic entitled Phylogenetic study of the genus Marasmiellus based on morphological and molecular analysis. Thirty five morphological characters of 37 taxa were scored manually for phylogenetic analysis based on morphological characters. Those taxa consisted of 35 species of Marasmiellus in Java and Bali found (topic 1), a type species of Marasmiellus (M. juniperinus), and Crinipellis brunneipurpurea as an outgroup. Matrix data was analyzed by Phylogenetic Analysis Using Parsimony (PAUP) program. The topology of phylogenetic tree based on morphological data was compared to topology of phylogenetic tree based on molecular data. The phylogenetic tree based on morphological and molecular characters showed that Marasmiellus spp. were divided into four major clades. Each clade consisted of several Singer?s sections. This result indicated that the sections within Singer?s infrageneric classification were polyphyletic. Morphological and molecular phylogenies resulted in this study did not support traditional classification, i.e. Singer?s classification of Marasmiellus. The phylogenetic relationship of Marasmiellus taxa, Gymnopus and its closely related genera were analyzed using molecular data of ITS rDNA. Forty one sequences based on ITS region representing of 36 species of Marasmiellus were used for phylogenetic analysis. Nine sequences of Marasmiellus were generated from this study, and 32 sequences were obtained from the international DNA database (GenBank). The phylogenetic tree showed that Marasmiellus taxa were divided into 4 clades. The taxa of Marasmiellus and Gymnopus were clustered together in three of four clades. The type species, Marasmiellus juniperinus was located within the same clade as Gymnopus fusipes. This result indicated that the genus Marasmiellus was polyphyletic. The third topic entitled Description of a novel species, Marasmiellus javanicus, based on morphological and molecular analysis. Marasmiellus javanicus is one of the 17 new species in this study, and it was described by using the integrated morphological and molecular data. Marasmiellus javanicus was described as a new species based on 5 specimens found in Bogor Botanical Garden, West Java. Morphologically it was similar to M. hondurensis (Murrill) Singer which was collected from Belice in 1906 by Peck (Singer 1973). The characters similarities of M. javanicus and M. hondurensis were off-white, same size of width, convex with flattened disc of pileus; subdistant lamellae; tomentose and insititious stipe; cutis with diverticulate of pileipellis, and presence of stipe vesture. Marasmiellus hondurensis was different from M. javanicus in having longer stipe (6?22 mm diam), fusoid basidiospores, Rotalis-type of cheilocystidia, and M. hondurensis was found on dicotyledon plant. At present, there is no sequence based on ITS rDNA of M. hondurensis. Thus, genetically M. hondurensis was unable to be compared to M. javanicus. To know the closest species of M. javanicus, phylogenetic tree of Marasmiellus was constructed. Phylogenetic tree showed that the most closely related species to M. javanicus was M. mesosporus. Marasmiellus javanicus and M. mesosporus have ITS rDNA 94% sequence similarity. They differ in 27 nucleotides which were 7 insertion and deletion, and 24 of substitution (A↔G=11, C↔T=13). This study showed that integrated morphological and molecular data are required to identify the Marasmiellus.

Abstrak :
ABSTRAK


Panas testis yang melebihi suhu skrotum akan menyebabkan perubahan pada testis. Sebagaimana diketahui banwa apapun bentuk panas, apabila dikenai pada testis akan bersifat merusak jaringan testis dan perkembangan sel-sel spermatogenik yang ada di dalam tubulus testis.

Pada penelitian ini akan diteliti pengaruh pemanasan testis secara berulang terhadap viabilitas dan morfologi spermatozoa mencit (Mus musculus L.) strain CBR.

Dari 50 ekor mencit yang berumur 3-4 bulan dan mempunyai berat badan sekitar 20-25 gram, dibagi menjadi lima kelompok. Kelima kelompok mencit tersebut adalah, kelompok kontrol tanpa periakuan (K1), kelompok kontrol yang dibius selama 10 menit (K2), kelompok pemanasan testis 390C (P1), kelompok pemanasan testis 40°C (P2) dan kelompok pemanasan testis 410C (P3). Lamanya pemanasan untuk setiap kelompok diberikan selama 10 menit. Perlakuan diulang sebanyak empat kali dengan selang waktu sembilan hari atau satu siklus epitel seminiferus. Kemudian mencit dimatikan dengan eter dan dibedah untuk diambil sperma yang tersimpan di dalam vas deferens. Selanjutnya spermatozoa yang dikeluarkan dari vas deferens dihitung jumlah prosentase viabilitas dan morfologi spermatozoa yang abnormal.

Dari uji statistik diperoleh hasil bahwa pemanasan testis pada suhu 39 C, 40 C, dan 41°C berpengaruh terhadap viabilitas dan morfologi spermatozoa. Sedangkan berat testis terpengaruh pada pemanasan 41°C.

Abstrak :
Skripsi ini ditulis untuk menganalisis verba triliteral dalam juz 30 Al-Qur’an dari segi morfologi dan sintaksis. Analisis ini merupakan analisis kualitatif dan desain deskriptif. Tujuan pengalisisan verba triliteral dalam juz 30 Al-Qur’an untuk memamparkan pola-pola verba triliteral yang paling banyak digunakan dan memaparkan analisis sintaksis dalam bahasa Arab. Data dalam skripsi ini didapatkan dari juz 30 Al-Qur’an dan dipersempit hanya dengan menganalisa verba triliteral. Teori yang digunakan dalam menganalisa morfologi dan sintaksis dalam bahasa Arab ini adalah teori morfologi dari Holes (1995) dan teori morfologi yang dikemukakan oleh Ahmed (2008). Hasil dari analisis ini dalam segi morfologi adalah pola-pola konjugasi verba triliteral yang paling banyak digunakan dalam juz 30 dan dari segi sintaksis dengan mengalisa klasifikasi verba, modus, dan struktur yang terdapat dalam juz 30 Al-Qur'an. ......This thesis was written to analyze the triliteral verbs in chapter 30 of Quran in terms of morphology and syntax. This analysis is an analysis of qualitative and descriptive design. Purpose of analyzing triliteral verbs in 30 chapters of the Quran to describe patterns of triliteral verbs which are the most widely used and explained in Arabic syntactical analysis. The data in this paper come from chapter 30 of Quran and narrowed only by analyzing the triliteral verbs. The theory used to analyze the morphology and syntax of the Arabic language are the theory of morphology of Holes (1995) and morphology theory proposed by Ahmed (2008). The results of this analysis in terms of morphology is verb conjugation patterns triliteral of the most widely used in terms of chapters 30 and by analyzing the syntactic classification of verbs, mood, and structure contained in the 30 chapters of the Qur'an.