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Abstrak :
ABSTRAK
Biodiesel dan biobutanol merupakan contoh sumber energi alternatif pengganti bahan bakar cair yang bersifat karbon netral dan mempunyai beberapa keunggulan dari segi lingkungan apabila dibandingkan dengan bahan bakar fosil. Biodiesel dapat diperoleh dari tanaman, dan juga mikroalga melalui proses ekstraksi. Sedangkan biobutanol dapat diperoleh melalui proses fermentasi. Penelitian ini bertujuan untuk mengetahui kemampuan bakteri C. acetobutylicum dalam memfermentasi mikroalga Nannochloropsis sp dalam menghasilkan biobutanol, serta pengaruhnya terhadap perolehan lipid. Fermentasi terhadap mikroalga Nannochloropsis sp untuk produksi biobutanol sebagai perlakuan awal sebelum ekstraksi lipid mikroalga. Fermentasi dilakukan menggunakan bakteri C. acetobutylicum selama 96 jam. Hasil yang diperoleh menunjukkan bakteri C. acetobutylicum mampu menghasilkan butanol 2.61 v/v sebagai hasil yang tertinggi. Proses tersebut menggunakan media hidrolisat dan biomassa mikroalga Nannochloropsis sp hasil hidrolisis viscozyme. Hasil ekstraksi lipid mikroalga Nannochloropsis sp menunjukkan hasil tertinggi dengan diperoleh lipid 25,4 per gram berat kering. Ekstraksi dilakukan pada hidrolisat dan biomassa hasil hidrolisis viscozyme yang tersisa setelah fermentasi.

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ABSTRACT
Biodiesel and biobutanol are the examples of alternative energy sources to replace liquid fuel with the carbon neutral characteristic. It also has other benefits related to the environment compared with fossil fuel. Biodiesel can be obtained from plant and microalgae through the extraction process, while biobutanol obtained through fermentation. Fermentation used carbohydrates of microalgae cells wall or other parts as energy and carbon sources. The aim of this study is to determine the ability of C. acetobutylicum bacteria in the fermentation of Nannochloropsis sp microalgae to produce biobutanol, as well as its effect on lipid acquisition. Fermentation of Nannochloropsis sp microalgae for biobutanol production as an initial treatment before lipid extraction. Fermentation was performed with C. acetobutylicum bacteria for 96 hours. The result showed that C. acetobutylicum bacteria were able to produce 2.61 v v butanol as the highest result. This process used Nannochloropsis sp microalgae hydrolysates and biomass of viscozyme hydrolysis yield. The result of Nannochloropsis sp microalgae extraction also showed the highest lipid content 25.4 per gram dry weight. This used hydrolysates and biomass of viscozyme hydrolysis that remaining after fermentation as media.

Abstrak :
Microalgae can bring solution for reducing CO2 and as alternative sustainable energy source. CO2 reduction by microalgae was done by fixation of CO2 during photosynthesis while they grow. The objective of the study is to get pH condition from CO2 injection into cultivated microalga that gives better productivity of biomass

Abstrak :
Research on the oil contents of microalgae i.e. Botryococcus braunii, Nannochloropsisand Spirulina platensisof different cultivation time have been done at Research Center for Marine and Fishery Product Processing and Biotechnology(RCMFPPB) Laboratory, Slipi, Jakarta. The three species of microalgae were cultivated outdoor in 100 L of seawater medium of 20 ppt salinity using sun light intensity and continuous aerations. Experiments were conducted in three replicates. Observations on the cell growth were carried out every 2 days and the biomass were harvested on day 5, 9 and 15 and sun-dried. Oil were extracted from the dry biomass using hexane. The highest cell density was reached by S. platensiswith 8.46 log cell/mL on day 13, while the highest growth rate was shown by S. platensiswith growth rate (k) = 9.40 on day 3. The highest yield of oil was obtained from B. brauniion day 9 which was 14.90%.

Abstrak :
ABSTRACT
Microalgae cultures recently have been used for various puposes. Culture development of microalgae started from the late 1800s and still continue untul now. Specific media composition, lighting, aeraton, culture system were applied to many different species microalgae for specific purposes such as pharmaceutical, healthcare, cosmetics, environmental management, energy production, and aquaculture.

Abstrak :
ABSTRAK
Air asam tambang (AAT) merupakan salah satu dampak negatif dari kegiatan pertambangan yang dapat menyebabkan masalah lingkungan dan memerlukan penanganan yang efisien dan efektif. Pemanfaatan mikroalga dalam remediasi atau fikoremediasi merupakan sebuah alternatif pengolahan AAT, namun memiliki keterbatasan aplikasi. Kendala pada aplikasi fikoremediasi AAT adalah karateristik dari air limbah yang membatasi pertumbuhan mikroalga, dimana air asam tambang memiliki kandungan logam yang tinggi, serta pH rendah. Penelitian ini dilakukan untuk mengetahui potensi pertumbuhan mikroalga Botryococcus braunii sebagai agen fikoremediasi AAT. Eksperimen dilakukan dengan melakukan kultivasi mikroalga Botryococcus braunii pada AAT pada 5 photobioreactor (PBR) dengan variasi konsentrasi AAT (v v) 0, 2,5, 3, 3,5, 4, dan dilakukan kajian hubungan antara pertumbuhan mikroalga dengan konsentrasi logam Fe dan Mn, serta pH pada AAT. Botryococcus braunii menunjukkan laju pertumbuhan berbeda, dengan nilai berurutan dari konsentrasi AAT terendah sebesar 0,0862 hari, 1,403 hari, 1,374 /hari, 0,0738 hari, dan 0,0616 hari, dengan variasi fase pertumbuhan. Penyisihan logam Fe dengan nilai berurutan dari konsentrasi AAT terendah sebesar 27,96, 6,98, 51,42, 79,45 dan 84,29, dan penyisihan logam Mn sebesar 27,96, 6,98, 51,42, 79,45 dan 84,29. Diketahui pula pH masing-masing media dengan dengan nilai berurutan dari konsentrasi AAT terendah sebesar 8,0, 7,7, 7,7, 7,5, dan 7,5. Hasil penelitian ini menunjukkan mikroalga Botryococcus braunii yang teraklimatisasi pada media AAT memiliki potensi sebagai bahan yang ekonomis dan berkelanjutan untuk menjadi agen remediasi AAT.

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ABSTRACT
Acid mine drainage (AMD) is one of negative impact on the mining industry, which can cause an environmental problem and requires an efficient and effective treatment system. Utilization of microalgae for remediation, or phycoremediation, is an alternative for AMD treatment, but it has limited applications. Limitation in phycoremediation is the characteristics of AMD that are not suitable for microalgae cultivation, due to its high metal concentration and low pH. This research was conducted by cultivating Botryococcus braunii microalgae on AMD with 5 photobioreactors (PBR) with variation in AMD concentration of (v v) 0, 2,5, 3, 3,5, 4, and the relationship between microalgae growth with Fe and Mn concentration and pH on AMD was conducted. Botryococcus braunii showed different growth rate with values sequentially from the lowest AMD concentration is 0,0862 day, 1,403 day, 1,374 day, 0,0738 day, and 0,0616 /day, with variations on growth phase. Removal efficiency of Fe with values sequentially from the lowest AMD concentration is 27,96, 6,98, 51,42, 79,45 and 84,29 and removal efficiency of Mn is 27,96, 6,98, 51,42, 79,45 and 84,29. It is also known pH value sequentially from the lowest AMD concentration is 8,0, 7,7, 7,7, 7,5, and 7,5. This research shows that Botryococcus braunii acclimatized on AMD media has the potential to become an economical and sustainable material for AMD remediation.

Abstrak :
Media culture is one of the important factors in the growth of mivroalgae. Culture medium contains macro and micro nutrition required for the growth of microalgae. Composition of nutrition concentrations determine the production of microalgae biomass and nutrient content. The aim of this study is to evaluate the effect of medium composition of media for microalgae Scenedesmus sp. This study was conducted in a laboratory scale. The growth medium that were used in this tudy were BBL sm, SS, MN, M4N, "Sederhana 2", and "Sederhana 3" medium. The microalgae that were used in this study were mixed culture Chlorophyceae microalgae which is dominated by scenedesmus sp. The parameters observed were pH, optical density (OD), cell density and temperature. the results show that of the 6 variations of the growth medium used in this study M4N medium is the best medium for the growth of microalgae. At the end of the operation, cell density of Scenedesmus sp. from M4N medium was 306.83 x 106 cell/ML with a dark green color for the culture. The coefficient growth of Scenedesmus sp. which is grown in M4N medium was 0,36. This is likely to be due to the composition of nutrients in the medium M4N which were more complete than other media.

Abstrak :
Marine resources as energy resources has become one means for the construction maritime power and unifying the region's defense for Indonesia. The geographical condition of Indonesia as an archipelagic country with the vast majority of territorial waters, as well as its strategic cross-position, becomes a fundamental source for sustainable energy development through the utilization of microalgae as a potential marine plant for biodiesel fuel, a diesel mix for Indonesian coastal communities. The research method in this study uses literature study and observation of previous authors' research, which will result in an analysis of energy objectives as supporting maritime defense that includes: (1) comprehensive understanding on the management and development of marine energy resources through the use of biodiesel from renewable energy of marine microalgae; (2) raising effective awareness and cooperation among the coastal communities, government and industry regarding the greatness of Indonesian history that has been built from the control over the oceans as a source of defense in developing energy security to support maritime defense.