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"Penelitian mengenai produksi biomassa Mastigocladus HS-46 pada medium NPK dengan penambahan variasi konsentrasi ekstrak tauge dalam sistem flat photobioreactor telah dilakukan. Optimalisasi kandungan makronutrien medium NPK sebagai pengganti Bold's Basal Medium (BBM) untuk menumbuhkan cyanobacteria dapat melalui penambahan kandungan ekstrak tauge. Mastigocladus HS-46 diisolasi dari sumber air panas Maribaya pada suhu lingkungan 42 oC. Medium yang digunakan untuk menumbuhkan Mastigocladus HS-46 terdiri atas medium BBM sebagai kontrol dan medium NPK 350 ppm dengan penambahan konsentrasi ekstrak tauge 1%, 2%, dan 3%. Penelitian bertujuan untuk mengetahui pengaruh penambahan variasi konsentrasi ekstrak tauge dalam medium NPK 350 ppm dan medium BBM terhadap produksi biomassa Mastigocladus HS-46. Selain itu, penelitian bertujuan untuk mengetahui perbedaan kadar lipid Mastigocladus HS-46 dalam medium. Hasil penelitian menunjukkan medium NPK 350 ppm dengan penambahan ekstrak tauge 3% menghasilkan berat biomassa dan lipid tertinggi Mastigocladus HS-46 dibandingkan dengan medium BBM dan NPK 350 ppm dengan penambahan ekstrak tauge 2% dan 1%. Medium NPK 350 ppm dengan penambahan ekstrak tauge 3% menghasilkan berat biomassa tertinggi sebesar 0,1632 g/mL dengan kadar lipid tertinggi sebesar 62 %.

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Research on Mastigocladus HS-46 biomass production on NPK medium with the addition of bean sprout extract with varying concentrations in flat photobioreactor system has been done. Optimization of the macronutrient content as a replacement to the Bold's Basal Medium (BBM) for cyanobacteria cultivation can be done with the use of bean sprout extract. Mastigocladus HS-46 was isolated from Maribaya Hot Spring at the temperature of 42 °C. The mediums used for Mastigocladus cultivation are BBM as control, and NPK mediums with the addition of bean sprout extract of 1%, 2% and 3% concentrations for the experimental group. The purpose of his research is to understand the effect of BBM and bean sprout extract addition with varying concentrations in 350 ppm NPK medium on Mastigocladus HS-46 biomass production. This research also aims to determine differences in the lipid content of Mastigocladus HS-46 in mediums. The results showed that 350 ppm NPK medium with 3% bean sprout extract addition produces the highest amount of biomass and lipid compared to the BBM and 350 ppm NPK medium with 2% and 1% bean sprout extract addition, producing 0,1632 g/ml of biomass and containing 62% lipid."

"ABSTRAKPenelitian mengenai pengaruh variasi konsentrasi medium NPK terhadap pertumbuhan Mastigocladus HS-46 telah dilakukan. Penelitian bertujuan untuk mengetahui pengaruh medium NPK terhadap berat biomassa. Selain itu, penelitian bertujuan untuk mengetahui konsentrasi medium NPK yang tepat agar menghasilkan berat biomassa yang didukung dengan kandungan protein dan lipid yang tinggi. Mastigocladus HS-46 ditumbuhkan menggunakan medium BBM sebagai medium kontrol dan medium NPK sebagai medium uji. Konsentrasi medium NPK yang digunakan, yaitu 80 ppm, 160 ppm, dan 240 ppm. Mastigocladus HS-46 diinkubasi pada suhu 35 C, intensitas cahaya 1500 mdash;3000 lux, dan pH awal medium sebesar 6,5. Pertumbuhan Mastigocladus HS-46 dilihat secara kualitatif berdasarkan kurva pertumbuhan dan secara kuantitatif berdasarkan uji statistik. Hasil penelitian menunjukkan bahwa Mastigocladus HS-46 yang ditumbuhkan dalam medium 240 ppm tumbuh lebih baik dari medium BBM, medium NPK 80 ppm, dan 160 ppm. Hal tersebut didukung dengan berat biomassa tertinggi pada hari ke-25 t25 sebesar 2,09 mg/mL-1 dan kandungan lipid tertinggi 57

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ABSTRACTThe study about the effect of variation concentration medium NPK to the Biomass Mastigocladus Cyanobacteria had been done. The aim of the study was to known the effect of variance concentration of NPK growth media within the biomass production. The other aim of the study was to determine the best concentration NPK growth media for Mastigocladus HS 46 to produce higher biomass with high protein and lipid content. Mastigocladus HS 46 was grown in BBM as a control and NPK growth media as working media. The variance concentration of NPK growth media that had been used for this study were 80 ppm, 160 ppm and 240 ppm. Mastigocladus HS 46 was incubated on 35 C with light intensity 1500 mdash 3000 lux and initial pH 6.5. The growth of Mastigocladus HS 46 was meausured with qualitative data based on growth curve and quantitative data based on statistic. The result showed that the best concentration for Mastigocladus HS 46 growth was in 240 ppm than BBM, NPK medium 80 ppm, and NPK medium 160 ppm. Based on the highest biomass production was 2.09 mg.mL 1 and lipid content was 57. "