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Abstrak :
Penelitian bertujuan untuk mengetahui identitas khamir yang hidup pada putik bunga Ceiba pentandra (L.) Gaertn dan saluran pencernaan Apis mellifera L., lebah pengumpul polen yang mengunjungi bunga Ceiba pentandra. Sebanyak 12 isolat khamir yang terdiri dari tiga isolat dari putik bunga Ceiba pentandra dan sembilan isolat dari saluran pencernaan Apis mellifera digunakan pada penelitian. Isolat-isolat khamir diidentifikasi berdasarkan hasil Basic Local Alignment Searching Tools (BLAST) data sequence daerah ITS rDNA, analisis filogenetik dengan metode Neighbor Joining, dan pengamatan alat reproduksi seksual dan aseksual. Primer forward ITS1 dan primer reverse ITS4 digunakan untuk mengamplifikasi daerah ITS rDNA. Hasil elektroforesis gel produk PCR menunjukkan ukuran daerah ITS rDNA isolat khamir tersebut bervariasi antara 400 hingga 800 pb. Hasil menunjukkan bahwa 12 isolat khamir terdiri dari enam species. Lima species khamir termasuk ke dalam phylum Ascomycota, order Saccharomycetales, class Saccharomycetes dan satu species khamir termasuk ke dalam phylum Basidiomycota, order Tremellales,dan class Tremellomycetes. Tiga isolat khamir dari putik bunga C. pentandra diidentifikasi sebagai Bullera coprosmaensis (JZ137), Candida orthopsilosis (JZ053), dan Debaryomyces hansenii (JZ051). Sembilan isolat khamir dari saluran pencernaan A. mellifera diidentifikasi sebagai Candida fermentatii (JZ059 dan JZ060), Candida mesorugosa (JZ057, JZ058, dan JZ063), Candida orthopsilosis (JZ064 dan JZ065), Candida parapsilosis (JZ066), dan Debaryomyces hansenii (JZ061). Dua species khamir yaitu Candida orthopsilosis dan Debaryomces hansenii ditemukan pada putik C. pentandra dan saluran pencernaan A. mellifera. ......The aim of this study was to identify yeasts from the pistils of Ceiba pentandra (L.) Gaertn and digestive tracts of pollen collecting bee, Apis mellifera L. Twelve yeast isolates were identified which were consisted of three isolates from the pistils of C. pentandra and nine isolates from digestive tracts of A. mellifera. Identification was based on homology sequences analysis using Basic Local Alignment Searching Tools (BLAST), phylogenetic analysis by Neighbor Joining method, and observation of sexual and asexual reproduction. The primer set of ITS1 (forward primer) and ITS4 (reverse primer) were used to amplify ITS region rDNA of the isolates. Gel electrophoresis results showed that the size of ITS region of the isolates were varied on the range of 400--800 bp. The results showed that twelve yeast isolates were identified as six species. Taxonomically, five species belong to phylum Ascomycota, order Saccharomycetales, class Saccharomycetes and one species belong to phylum Basidiomycota order Tremellales, class Tremellomycetes. Three yeast isolates from the pistils of C. pentandra were identified as Bullera coprosmaensis (JZ137), Candida orthopsilosis (JZ053), and Debaryomyces hansenii (JZ051). Nine yeast isolates from digestive tracts of pollen collecting A. mellifera were identified as Candida fermentatii (JZ059 & JZ060), Candida mesorugosa (JZ057, JZ058, dan JZ063), Candida orthopsilosis (JZ064 & JZ065), Candida parapsilosis (JZ066), and Debaryomyces hansenii (JZ061). Debaryomyces hansenii and Candida orthopsilosis were found on the pistils of C. pentandra and digestive tracts of A. mellifera.

Abstrak :
Penelitian bertujuan mengetahui identitas khamir dari saluran pencernaan lebah pekerja pengumpul polen (pollen collecting bees, PCB) Apis mellifera. Sebanyak 12 isolat khamir dari saluran pencernaan PCB yang mengunjungi bunga kapuk Ceiba pentandra di Jepara, Jawa Tengah, diidentifikasi berdasarkan data sequence daerah internal transcribed spacer (ITS) rDNA. Amplifikasi daerah ITS rDNA menggunakan primer forward ITS1 dan primer reverse ITS4. Elektroforesis produk PCR menunjukkan bahwa daerah ITS rDNA khamir-khamir tersebut berukuran antara 400--900 pb. Berdasarkan hasil pencarian homologi sequence menggunakan program basic local alignment search tool (BLAST), analisis filogenetik menggunakan metode Neighbor Joining (NJ), dan karakterisasi morfologi, 12 isolat khamir tersebut terdiri dari tujuh spesies yang termasuk dalam lima genus. Secara taksonomi, seluruh khamir tersebut termasuk phylum Ascomycota, class Hemiascomycetes, dan order Saccharomycetales. Isolat-isolat tersebut diidentifikasi sebagai Candida magnoliae (isolat JZ002), Candida orthopsilosis (isolat JZ003, JZ008, JZ011, dan JZ034), Candida rugosa (isolat JZ010); Debaryomyces hansenii (isolat JZ001); Meyerozyma caribbica (isolat JZ013 dan JZ014), Pichia guilliermondii (isolat JZ015), dan Zygosaccharomyces siamensis (isolat JZ005 dan JZ006). ......The aim of this study was to obtain the identity of yeasts from digestive tracts of pollen collecting bees (PCB) Apis mellifera. A total of 12 yeast isolates obtained from digestive tract of PCB foraging on flowers Ceiba pentandra in Jepara, Central Java, were identified based on sequence data of internal transcribed spacer of ribosomal DNA (ITS rDNA). The primer set of ITS1 (forward primer) and ITS4 (reverse primer) were used to amplify the ITS region rDNA. Gel electrophoresis result showed that the size of ITS rDNA of those yeast were varied between 400--900 base pairs. Based on sequence homology search using basic local alignment search tool (BLAST) program, phylogenetic analysis by Neighbor Joining method, and morphological characterization, those 12 isolates belong to five genera and seven species. Taxonomically, all of those isolates belong to order Saccharomycetales, class Hemiascomycetes from the phylum Ascomycota. Those 12 isolates were identified as species Candida magnoliae (isolate JZ002); Candida orthopsilosis (isolates JZ003, JZ008, JZ011, and JZ034); Candida rugosa (isolate JZ010); Debaryomyces hansenii (isolate JZ001); Meyerozyma caribbica (isolates JZ013 and JZ014); Pichia guilliermondii (isolate JZ015); and Zygosaccharomyces siamensis (isolates JZ005 and JZ006).