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Abstrak :
Inoculum for making tempe is usually made from the mould Rhizopus oryzae or Rhizopus oligospons. Large scale use of an inoculum from one source only (LKN-LIPI) might threaten the biodiversity of microorganisms in traditionally made inoculum. The potential use of other Rhizopus moulds as inoculum for tempe production should be examined. Rh. microsporus var. rhizopodifonnis UICC 520 isolated from Hibiscus from Manado and Rh. microsporus var. chinensis UICC 521 isolated from tempe from Aceh were examined for their potentials to produce tempe. Rhizopus has also been known to be able to transform antioxidants such as isoflavone in tempe. The potential of Rhizopus microsporus v. Tiegh. var. chinensis (Saito) UICC 521 to transform glucoside isoflavones in soybean tempe has been evaluated. The results showed that Rh. microsporus var. rhizopodiformis UICC 520 has little potential as a tempe inoculum. Rh. microsporus var. chinensis UICC 521, however, is very potential as an inoculum to produce good tempe and produces abundant spores ((3-10)x106 cell/ml) on rice substrate. Two types of aglycon isoflavone, daidzein and genistein, were detected by TLC separation. The total isoflavone concentration during fermentation period of 24, 48, 72 hours was determined using HPLC. At 24 hours the concentration was 0.142 mg/g tempe, while at 48 and 72 hours the concentration were 0.181 and 0.135 mg/g tempe, respectively. The highest total isoflavone concentration was detected at 48 hours fermentation period. Hence, there was an increase of aglycon isoflavones up to 6000% after transformation of isoflavones in soybean by Rh. microsporus var. chinensis UICC 521 when compared to the initial concentration before transformation.

Abstrak :
Kacang kedelai merupakan sumber isoflavon terbanyak dan salah satu produk olahannya ialah susu kacang kedelai. Penelitian ini bertujuan untuk mengetahui efek pemberian susu kacang kedelai terhadap kadar glukosa darah mencit putih jantan galur ddY yang dibebani glukosa. Penelitian ini menggunakan rancangan acak lengkap dengan 25 ekor mencit putih jantan galur ddY yang terbagi dalam 5 kelompok, yaitu kontrol normal (CMC 0,5% 0,5 ml/20 g BB), kontrol pembanding (Metformin HCl 13 mg/20 g BB), dan 3 variasi dosis uji (0,325 g kedelai/20 g BB; 0,65 g kedelai/20 g BB; 1,3 g kedelai/20 g BB) yang diberikan dalam bentuk susu kacang kedelai. Mencit terlebih dahulu diukur kadar glukosa darah puasa, kemudian diberikan larutan uji. Tiga puluh menit setelah perlakuan, kadar glukosa darah diukur kembali, kemudian diberikan glukosa 2 g/kg BB per oral. Pengukuran kadar glukosa darah dilakukan pada menit ke-30, 60, 90, 120 setelah pembebanan glukosa. Kadar glukosa darah diukur dengan menggunakan glukometer ACCU-CHEK® Active. Pemberian susu kacang kedelai dapat menurunkan kadar glukosa darah mencit putih jantan galur ddY yang dibebani glukosa pada semua dosis (0,325; 0,65; 1,3 g kacang kedelai/20 g BB mencit), namun penurunan kadar glukosa darah yang terbaik terlihat pada dosis 1 (0,325 g kacang kedelai/20 g BB mencit).

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Soybean is most abundant source of isoflavones and one of soy products is soybean milk. This study was made to investigate the effect of soybean milk administration towards blood glucose level in glucose loaded male ddY mice. A completely randomized design was conducted using 25 male ddY mice that were divided into 5 groups; normal control (CMC 0,5% 0,5 ml/20 g b.w.), drug control (Metformin HCl 13 mg/20 g b.w.), and 3 different treatment doses (0,325 g soybean/20 g b.w.; 0,65 g soybean/20 g b.w.; 1,3 g soybean/20 g b.w.) which were given in soybean milk. Fasting blood glucose was measured and mice were treated based on their groups. Thirty minutes after treatment, blood glucose level was measured again and then mice were loaded glucose 2 g/kg b.w. orally. Blood glucose level was measured at 30, 60, 90, and 120 minutes postload glucose. Blood glucose level was measured by using ACCU-CHEK® Active meter. Administration of soybean milk lowered blood glucose level in glucose loaded male ddY mice treated with 0,325; 0,65; 1,3 g soybean/20 g b.w., but treatment with 0,325 g soybean/20 g b.w. showed the best reduction of blood glucose level.