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Abstrak :
Hem merupakan komponen penyusun hemoprotein, salah satunya yaitu sitoglobin. Sitoglobin diketahui memegang peranan dalam perkembangan kanker. Saat ini, belum diketahui peran hambatan hem terhadap ekspresi CYGB pada sel lini sel kanker hati, HepG2 Penelitian ini bertujuan untuk melihat kemampuan penghambatan hem dalam mencegah proliferasi sel HepG2. Penghambatan hem dilakukan dengan menggunakan suksinil aseton.  Analisis aktivitas enzim ALAD diukur secara kolorimetrik. Analisis viabilitas dan proliferasi (doubling time) dilakukan dengan menggunakan MTT assay. Analisis ekspresi mRNA CYGB dilakukan dengan qRT-PCR. Ekspresi protein CYGB dianalisis dengan ELISA. Hasil yang diperoleh adalah hambatan sintesis hem pada sel HepG2 dengan menggunakan suksinil aseton berhasil dilakukan. Penurunan sintesis hem berdampak pada menurunnya ekspresi CYGB baik tingkat mRNA maupun protein. Viabilitas dan proliferasi sel HepG2 menurun seiring dengan meningkatnya konsentrasi suksinil aseton. Sebagai kesimpulan, pemberian suksinil aseton mampu menghambat sintesis hem karena menekan ekspresi CYGB yang berdampak pada penurunan viabilitas dan proliferasi sel HepG2. ......Hem is a component of hemoprotein, one of which is cytogloblin. Cytoglobin is known to play a role in cancer development. Currently, the role of heme inhibitors on CYGB expression in the liver cancer cell line, HepG2, is unknown. This study aims to see the ability of heme inhibition in preventing HepG2 cell proliferation. Hem inhibition was carried out using succinyl acetone. Analysis of ALAD enzyme activity was measured colorimetrically. Viability and proliferation (doubling time) analyzes were performed using the MTT assay. Analysis of CYGB mRNA expression was performed by qRT-PCR. CYGB protein expression was analyzed by ELISA. The results obtained were thatinhibition of hem synthesis in HepG2 cells using succinyl acetone was successfully carried out. Decreased heme synthesis resulted in decreased CYGB expression both at the mRNA and protein levels. HepG2 cell viability and proliferation decreased with increasing succinyl acetone concentration. In conclusion, succinyl acetone was able to inhibit hem synthesis cause it suppressed CYGB expression which had an impact on reducing the viability and proliferation of HepG2 cells.

Abstrak :
Penelitian ini bertujuan untuk mengetahui faktor dominan terhadap kecukupan asupan zat besi pada mahasiswa S-1 Reguler Angkatan 2013 Rumpun Ilmu Kesehatan Universitas Indonesia Tahun 2014. Desain yang digunakan adalah cross sectional, melibatkan 290 (perempuan=214; laki-laki=76) mahasiswa S-1 Reguler Angkatan 2013 RIK UI yang berusia 17-20 tahun pada April-Mei 2014. Metode pengambilan sampel adalah proporsional cluster random sampling. Pengumpulan data dilakukan melalui pengisian kuesioner dan wawancara asupan makanan 2x24 jam. Hasil penelitian menunjukan bahwa 83.8% responden tidak tercukupi kebutuhan zat besinya. Rata-rata asupan zat besi pada perempuan dan laki-laki adalah 12.3 mg/hari dan 16.0 mg/hari. Uji chi square menunjukan terdapat hubungan yang bermakna antara jenis kelamin (OR=7.56), perilaku konsumsi heme dan non heme (OR=2.86), konsumsi suplemen zat besi (OR=4.73), persepsi citra tubuh (OR=2.38), dan keterpaparan media massa (OR=3.01) terhadap kecukupan asupan zat besi. Analisis regresi logistik ganda menunjukan bahwa jenis kelamin (OR=19.17) dan konsumsi suplemen zat besi (OR=11.28) merupakan faktor dominan kecukupan asupan zat besi pada mahasiswa S-1 Reguler Angkatan 2013 RIK UI Tahun 2014.

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The objective of this study is to identify iron sufficiency's dominant factor among College Students of Health Sciences Program Batch 2013, Universitas Indonesia in 2014. This study used cross sectional design which conducted on 290 respondents (female=214; male=76) of college students of Health Sciences Program batch 2013, Universitas Indonesia, April-May 2014. Subjects aged in 17-20 years old and was performed by Proporsionate Cluster Random Sampling. Data were collected through the questionnaire and iron intake was obtained by 2x24 hours food recall. This study showed that 83.8% respondents who consumed iron unsufficient. Iron intake among female respondent was 12.3 mg/day and male was 16.0 mg/day. Chi Square Analysis showed significant relation between sex (OR=7.56), heme and non heme consumption (OR=2.86), iron supplement consumption (OR=4.73), body image (OR=2.38), and mass media (OR=3.01) with iron sufficiency. Regression Binary Logistic Analysis also showed that sex (OR=19.17) and iron supplement consumption (OR=11.28) as iron sufficiency's dominant factor among college students of Health Sciences Program Batch 2013, Universitas Indonesia in 2014.

Abstrak :
Bahan dan Metode : Desain cross seksional pada 99 subyek laki-laki tahun yang dipilih secara simple random sampling dari sarnpel MONICA Jakarta III. Data yang dikumpulkan meliputi data umum subyek, asupan makanan, antropometri, tekanan darah, EKG dan pemeriksaan laboratorium darah. Uji statistik yang digunakan adalah uji X2, Fisher dan Kolmogorov-Smimov, Mann Whitney dan korelasi Pearson / Spearman rank. Hasil : Kadar feritin serum ?200 p.glL tedapat pads 8,1% subyek. Asupan besi total 4,81 mg (1,59-13,24 mglhari), besi hem 0,21 mg (0-1,22 mg/had), 93,9% asupan besi kurang 1 AKG. Terdapat 13,1% dengan IMT >27 kglm2, 20,2% dengan Lpe X94 cm aan rasio LpelLpa X0,95; 34,3% dengan tekanan darah >149190 mm Hg, Kadar kolesterol total abnormal 41,4% (?200 mgldL); kolesterol HDL abnormal 63,6%(z40 mgldL); kolesterol LDL abnormal 52,5% (?130 mgldL); trigiiserida abnormal 11,I%(200 mg/dL); gula puasa abnormal 5,1% (?126 mgldL). Kebiasaan merokok pada 54,5% subyek. Tidak trdapat korelasi bermakna antara asupan besi total (r--0,038) dan besi hem (r,027) dengan feritin serum. Rasio Odds kasar antara feritin serum dengan PJK (diagnostik EKG) 5,5 kali (CI. 0,87-34,33). Pada uji statistik didapat perbedaan bermakna median feritin serum pads subyek diabetes daengan non diabetes (p~,001) dan subyek dengan kelebihan lemak tubuh dengan subyek dengan lemak tubuh normal (Lpe dengan p:1,009; LpelLpa dengan p"0,047). Kesimpulan: Didapatkan hubungan tidak bermakna antara feritin serum dengan asupan zat gizi. Terdapat hubungan moderat antara feritin serum dengan risiko PJK. Subyek dengan feritin serum ? 200 .iglL mempunyai kecenderungan risiko 5,5 kali menderita PJK (diagnostik EKG) dibandingkan subyek dengan feritin serum <200 p.g(L,

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Serum ferritin in men 35 years old or over and its relating factors at Mampang PrapatanMethods : A cross sectional study had been carried out of on 99 subjects age 35 years selected using simple random sampling method from MONICA Jakarta's III sample. Data collected consist of socio-economic state, dietary intake, anthropometric, laboratory, blood pressure and electrocardiogram examination. Statistical analysis was performed by X-, Fisher, Kolmogorov-Sm imov, Mann-Whitney, and Pearson/ Spearman rank correlation. Result : Serum ferritin 1200 1.tglL was found in 8,1% subjects. Total iron intake 4,81 mg (1,59-13,24 mg/day), heme iron 0,21 mg (0-1,22 mg/day), 93,9%% of iron intake below the RDA. There were 13,1% subjects with BMI >27 kg/m2; 20,2% with AC >94 cm and WHR >0,95; 34,5% with blood pressure >140/90 mm Hg. Abnormal total cholesterol level 41,4% (1200 mg/dL); abnormal HDL cholesterol 63,6% (<40 mg/dL); abnormal LDL cholesterol 52,5% (1130 mg/dL); abnormal triglyceride 1,1% (~0d mg/dL); abnormal fasting glucose 5,1% (?126 mgldL); 54,5% had smoking habits. Lack association between total iron (r=-0,038) and heme iron (r 0,027) with serum ferritin. Men with ferritin serum 1200 l.tg1L had an crude odds ration 5,5 fold suffer from CHD (according to ECG diagnostic) compare to subjects with ferritn serum <200 .iglL (CI. 0,87-34,33). Statistical analysis showed significant difference of serum ferritin median in diabetic and non diabetic subjects (p:1,001), overfatness subjects and normo fatness subjects (AC with. pC,009 and WHR with p=0,047). Conclusion : There is no significant relationship between serum ferritin level and dietary intake. Bivariate analysis found moderate relationship between serum ferritin and CHD. Men with serum ferritin 1200 pglL had a crude odds ratio 5,5 fold suffer from CHD (according to ECG diagnostic) compare to the subjects with serum ferritin < 200 pg/L.

Abstrak :
Anemia gizi besi masih merupakan masalah kesehatan masyarakat dengan prevalensi pada anak 5 - 12 tahun sebesar 29% di Indonesia dan di Kota Makassar sebesar 37,6%. Penelitian ini bertujuan untuk mengetahui faktor determinan (status kecacingan, status seng, kebiasaan sarapan pagi, pola konsumsi makanan sumber heme dan nonheme, pola konsumsi sumber makanan pelancar dan penghambat zat besi) terhadap kejadian anemia. Desain penelitian yang digunakan adalah potong lintang yang dilaksanakan pada bulan April ? Juni 2014. Penelitian ini menggunakan desain potong lintang yang dilaksanakan pada siswa kelas 3 - 5 SD Negeri Cambaya Kecamatan Ujung Tanah Kota Makassar. Sampel sebanyak 120 siswa yang dipilih secara acak sederhana. Analisis data dilakukan secara univariat, bivariat dengan uji kai kuadrat dan multivariat dengan regresi logistik. Hasil penelitian menunjukkan faktor determinan kejadian anemia adalah status kecacingan (nilai p = 0,007), kebiasaan sarapan pagi (nilai p = 0,002), pola konsumsi makanan sumber heme (nilai p = 0,004), dan pola konsumsi sumber makanan penghambat zat besi (nilai p = 0,016). Hasil analisis multivariat menunjukkan bahwa pola konsumsi makanan sumber heme (OR = 5,09 dan 95% CI = 1,98 ? 13,08) dan pola konsumsi sumber makanan penghambat zat besi (OR = 4,53 dan 95% CI = 1,65 ? 12,43) adalah determinan utama kejadian anemia gizi.

Iron deficiacy anemia has been a public health problem with prevalence on 5 - 12 year old children worth 29% in Indonesia and 37.6% in Makassar. This study aimed to determine the determinant factors (worm status, zinc status, breakfast habit, consumption pattern of heme and nonheme source of food, consumption pattern of iron enhancer and inhibitor food) toward anemia incidence. The study used cross sectional design conducted in April - June 2014. The population was third to fifth grade students of Cambaya State Elementary School at Ujung Tanah District , Makassar City. Sample of 120 students were selected randomly. Data was analyzed using univariate, bivariate with chi-square test, and multivariate with logistic regression test. The results showed that the determinant factors of anemia incidence were wormy status (p value = 0.007), breakfast habits (p value = 0.002), consumption pattern of heme and non-heme source of food (p value = 0.004), and consumption pattern of iron enhancer and inhibitor (p value = 0.016). Multivariate analysis result showed that consumption pattern of heme (OR = 5.09 and 95% CI = 1.98 - 13.08) and consumption pattern of iron enhancer and inhibitor food (OR = 4.53 and 95% CI = 1. 65 - 12.43) was a major determinant of nutritional anemia.