Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :

Latar Belakang: Periodontitis disebabkan oleh infeksi mikroba sepertiStreptococcus sanguinisyang mengganggu respon imun dan integritas jaringan pendukung gigi.Ekstrak etanol kelopak bunga rosela (Hibiscus sabdariffa Linn.) memiliki kandungan antimikrobial terhadap bakteri Gram-positif seperti S. sanguinis. Untuk mengembangkan bentuk sediaan, dibuat gel ekstrak etanol kelopak bunga rosela.Tujuan: Mengetahui efektivitas antibakteri gel ekstrak etanol kelopak bunga rosela terhadap S. sanguinis. Metode: Studi in vitro metode difusi agar yang mengukur zona hambat (mm) gel rosela 10%, 15%, dan 25% yang dipaparkan pada agar Mueller Hinton terinokulasi bakteri S. sanguinis, diinkubasi 6 jam, serta metode total plate count (CFU/mL) untuk menghitung jumlah koloni hidup bakteri S. sanguinis setelah terpapar gel rosela 10%, 15%, dan 25%. Kontrol positif yaitu gel klorheksidin 0,2% dan kontrol negatif yaitu gel basis tanpa zat aktif. Hasil: Zona hambat gel ekstrak etanol kelopak bunga rosela konsentrasi 15% dan 25% berbeda bermakna dibandingkan kontrol (p<0,05), gel konsentrasi 10% tidak menghasilkan zona hambat. Ketiga konsentrasi gel secara signifikanmenurunkan jumlah koloni bakteri dibandingkan kontrol (p<0,05). Efek penghambatan terbesar terdapat pada gel konsentrasi 25%. Kesimpulan: Gel ekstrak etanol kelopak bunga rosela memiliki efek hambat terhadap bakteri S. sanguinis.

---

Background: Periodontitis is caused by microbial infection, such as Streptococcus sanguinisthat disturbs immune response and the integrity of tooth-supporting tissue. Roselle (Hibiscus sabdariffa Linn.) calyx ethanol extract has antibacterial properties against Gram-positive bacteria, including S. sanguinis. In order to develop the dosage form, roselle calyx ethanol extract gel was made. Objective:To observe the antibacterial effectiveness of roselle calyx ethanol extract gel against S. sanguinis. Method: in vitrostudy using disk diffusion method which measures clear zone of inhibition (mm)of 10%, 15%, and 25% roselle gel applied on Mueller Hinton agar inoculated with S. sanguinis, incubated for 6 hours, and total plate count method which counts the number of living S. sanguiniscolonies (CFU/mL)after being exposed to 10%, 15%, and 25% roselle gel. Positive control is 0.2% chlorhexidine gel and negative control is gel without active substances.Result: Inhibitory zones of 15% and 25% roselle gel have significant differences compared controls (p<0.05), 10% roselle gel did not show inhibitory zones.All three concentrations of gel significantly reduced the number of colonies compared to controls (p<0.05). Highest inhibitory effect was observed in 25% roselle gel.Conclusion: roselle calyx ethanol extract gel showed inhibitory effect against S. sanguinis.

Abstrak :
Pendahuluan: Salah satu bakteri penyebab periodontitis adalah Treponema denticola. Untuk perawatan penyakit periodontal diperlukan aplikasi terapi topikal antibakteri tambahan untuk mempercepat penyembuhan dibanding hanya dengan terapi tunggal seperti kuretase. Ekstrak tanaman rosela (Hibiscus sabdariffa Linn.) memiliki khasiat antibakteri yang dapat melawan bakteri Gram negatif seperti Treponema denticola. Dalam upaya pengembangan bentuk sediaan, ekstrak etanol kelopak bunga rosela dibuat dalam bentuk sediaan gel. Tujuan: Mengetahui efektivitas gel ekstrak etanol kelopak bunga rosela (Hibiscus sabdariffa Linn.) sebagai antibakteri terhadap Treponema denticola. Metode: Uji zona hambat dilakukan dengan meletakkan cakram kertas yang dipaparkan gel ekstrak etanol kelopak bunga rosela 10%, 15%, 25%, kontrol positif, dan kontrol negatif di atas medium mueller-hinton agar yang telah diinokulasi bakteri Treponema denticola lalu diinkubasi dalam waktu 6 jam dengan suhu 37^oC pada kondisi anaerob. Uji total plate count dilakukan dengan menghitung jumlah koloni Treponema denticola yang masih hidup setelah dipaparkan dengan gel ekstrak etanol kelopak bunga rosela 10%, 15%, 25% serta kontrol positif dan kontrol negatif. Hasil: Gel ekstrak etanol kelopak bunga rosela konsentrasi 15% dan 25% menunjukkan efek zona hambat terhadap bakteri Treponema denticola. Pada uji total plate count, gel ekstrak etanol kelopak bunga rosela (Hibiscus sabdariffa Linn.) dengan konsentrasi 10%, 15%, dan 25% menunjukkan pengurangan jumlah koloni bakteri Treponema denticola. Kesimpulan: Gel ekstrak etanol kelopak bunga rosela (Hibiscus sabdariffa Linn.) konsentrasi 25% terbukti paling efektif dalam menghambat pertumbuhan bakteri Treponema denticola ATCC 33520. ......Introduction: One of the bacteria that causes periodontitis is Treponema denticola. For the treatment of periodontal disease, an additional antibacterial therapy by topical application to accelerate healing is needed rather than a single therapy such as curettage. The ethanol extract of the rosella plant (Hibiscus sabdariffa Linn.) has an antibacterial agent that can against Gram-negative bacteria such as Treponema denticola. In an effort to develop the dosage form, ethanol extract of roselle calyx was made in gel form. Objective: To investigate the efficacy of gel ethanol extract of roselle calyx (Hibiscus sabdariffa Linn.) as an antibacterial against Treponema denticola. Methods: Inhibition zone test was carried out by placing paper discs exposed with 10%, 15%, 25% rosella calyx extract gel ethanol, positive control, and negative control on the mueller-hinton agar medium which had been inoculated with T. denticola bacteria and then incubated within 6 hours with a temperature of 37^oC in anaerobic conditions. Total plate count test is done by counting the number of T. denticola colonies that are still alive after being exposed with 10%, 15%, 25% rosella calyx extract gel as well as positive and negative controls. Result: The gel ethanol extract of roselle calyx (Hibiscus sabdariffa Linn.) concentration of 15% and 25% showed the effect of inhibitory zones on the bacterium Treponema denticola. In the total plate count test, gel ethanol extract of roselle calyx (Hibiscus sabdariffa Linn.) with a concentration of 10%, 15%, and 25% showed reduction in the number of Treponema denticola colonies. Conclusion: The gel ethanol extract of roselle calyx (Hibiscus sabdariffa Linn.) concentration of 25% was proven to be the most effective in inhibiting the growth of the bacterium Treponema denticola ATCC 33520.