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"The research was conducted to determine the effect of ascorbic acid (50 mgl-1, 100 mgl-1, 200 mgl-1) and activated charcoal (0.5 gl-1, 1 gl-1, 2 gl-1) independently with different light duration (4 weeks in darkness, 2 weeks in darkness followed by 2 weeks in 16 hours light and 4 weeks in 16 hours light) on shoot regeneration. Explants of banana cultivar Barangan (Musa acuminata L.) were planted on MS basal media supplemented with 1.6 mgl-1 IAA, 4.0 mgl-1 BAP and cultured for 4 weeks. After 4 weeks, explant browning level was evaluated. Explants were then cut vertically into two pieces and planted on the same media to induce shoot regeneration. After 4 weeks in shoot regeneration media, number of shoot, colour of shoot and height of shoot were evaluated. MS media supplemented with 1.6 mgl-1 IAA and 4.0 mgl-1 BAP without ascorbic acid and activated charcoal in darkness for 4 weeks was the most suitable media for shoot regeneration. The shoot regeneration gave average of 10,4 shoots per explant.

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The research was conducted to determine the effect of ascorbic acid (50 mgl-1, 100 mgl-1, 200 mgl-1) and activated charcoal (0.5 gl-1, 1 gl-1, 2 gl-1) independently with different light duration (4 weeks in darkness, 2 weeks in darkness followed by 2 weeks in 16 hours light and 4 weeks in 16 hours light) on shoot regeneration. Explants of banana cultivar Barangan (Musa acuminata L.) were planted on MS basal media supplemented with 1.6 mgl-1 IAA, 4.0 mgl-1 BAP and cultured for 4 weeks. After 4 weeks, explant browning level was evaluated. Explants were then cut vertically into two pieces and planted on the same media to induce shoot regeneration. After 4 weeks in shoot regeneration media, number of shoot, colour of shoot and height of shoot were evaluated. MS media supplemented with 1.6 mgl-1 IAA and 4.0 mgl-1 BAP without ascorbic acid and activated charcoal in darkness for 4 weeks was the most suitable media for shoot regeneration. The shoot regeneration gave average of 10,4 shoots per explant."

"ABSTRAKMelastoma malabathricum L. merupakan tumbuhan yang berpotensi untuk dikembangkan menjadi tanaman fitoremediasi. Perbanyakan tumbuhan M. malabathricum sebagai objek penelitian lanjutan diperlukan untuk mengembangkan potensi yang ada. Perbanyakan M. malabathricum dapat dilakukan melalui kultur daun secara in vitro pada medium MS dengan kombinasi Thidiazuron TDZ dan 1-Naphthaleneacetic Acid NAA . Penelitian dilakukan untuk mengetahui respons eksplan daun M. malabathricum yang dikultur pada medium MS dengan penambahan kombinasi TDZ 0 mgl-1; 0,1 mgl-1; 1 mgl-1; 2 mgl-1 dan NAA 0 mgl-1; 0,1 mgl-1; 1 mgl-1 . Hasil pengamatan menunjukkan bahwa eksplan daun M. malabathricum dapat merespons medium perlakuan dengan membentuk kalus, kecuali pada medium dengan kombinasi 2 mgl-1 TDZ dan 1 mgl-1 NAA. Hasil pengamatan pada pekan ke-8 setelah penanaman menunjukkan bahwa kalus yang terbentuk cenderung memiliki tekstur remah kompak hingga kompak, dengan pencokelatan cenderung terjadi pada kalus yang terbentuk di medium dengan penambahan NAA tunggal 0,1 mgl-1; 1 mgl-1 . Penggunaan 1 mgl-1 NAA serta 0,1 mgl-1 TDZ memberikan hasil tertinggi dalam persentase eksplan yang membentuk kalus 100 . Rerata hari pembentukan kalus tercepat 6,25 hari terdapat pada medium dengan penambahan 1 mgl-1 NAA. Hasil pengamatan juga menunjukkan bahwa eksplan dapat membentuk kalus dan akar pada medium dengan penambahan NAA tunggal. Medium dengan penambahan 1 mgl-1 NAA memberikan hasil terbaik dalam menginduksi pembentukan akar pada eksplan daun M. malabathricum. Lebih lanjut, terdapat satu eskplan pada medium dengan kombinasi 2 mgl-1 TDZ dan 0,1 mgl-1 yang mampu membentuk kalus dan tunas.

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ABSTRACTMelastoma malabathricum L. is a plant that has the potential to be developed into phytoremediation plants. Propagation of M. malabathricum as a further research object is needed to develop the existing potential. Thus, can be done through in vitro culture of leaves in MS medium with the combination of Thidiazuron TDZ and 1 Naphthaleneacetic Acid NAA . This study was conducted to investigate the response of M. malabathricum leaf, when cultured on MS medium with the combination of TDZ 0 mgl 1, 0,1 mgl 1, 1 mgl 1 and 2 mgl 1 and NAA 0 mgl 1 0.1 mgl 1 and 1 mgl 1 . The results show that M. malabathricum leaf explants could respond to treatment medium by forming callus, except on medium with combination of 2 mgl 1 TDZ and 1 mgl 1 NAA. The results showed that the callus tended to have a friable compact and compact texture at 8th week, with browning tends to occur in callus formed on medium with the addition of single NAA 0.1 mgl 1 1 mgl 1 . The use of 1 mgl 1 NAA and 0.1 mgl 1 TDZ gave the highest results in the percentage of explants forming callus 100 . The average of the fastest callus forming time 6.25 days was found in the medium with the addition of 1 mgl 1 NAA. The result also show that explants could be forming callus and roots on a medium with the addition of a single NAA. Medium with addition of 1 mgl 1 NAA gave the best result in inducing root formation on M. malabathricum leaf explants. Moreover, there was one explant on the medium with a combination of 2 mgl 1 TDZ and 0.1 mgl 1 that capable to forming callus and shoots."

"Produksi benih secara in vitro dapat dijadikan metode alternatif dalam perbanyakan benih sumber kultivar unggul, namun penelitian penggunaan zat pengatur tumbuh yang tepat dalam mendukung regenerasi dan enkapsulasi pada kultivar ini belum pernah dilaporkan. Penelitian ini bertujuan untuk mendapatkan kombinasi TDZ dan NAA pada media dasar MS dan vitamin dari media B5 terbaik dalam regenerasi eksplan embryonic axis dan menentukan apakah metode enkapsulasi yang digunakan dapat mengenkapsulasi eksplan kedelai kultivar Rajabasa secara in vitro dengan baik. Penelitian dilaksanakan di Laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran, yang berlangsung dari bulan Mei hingga Agustus 2014. Eksplan yang digunakan adalah embrionic axis kedelai kultivar Rajabasa. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap. Media yang digunakan adalah MS dan Vitamin dari media B5 dengan penambahan zat pengatur tumbuh TDZ (0 mg L-1; 0,01 mg L-1; 0,1 mg L-1; 1,0 mg L-1) dan NAA (0 mg L-1; 0,01 mg L-1; 0,1 mg L-1; 1,0 mg L-1), kemudian tahap kedua dilakukan enkapsulasi pada eksplan hasil regenerasi menggunakan Na-Alginat 4% + CaCl2.2H2O 100 mM. Perlakuan yang terbaik diperoleh pada perlakuan TDZ 0,01 mgL-1 + NAA 0 mgL-1, tetapi tahap enkapsulasi yang dilakukan belum mampu mengenkapsulasi eksplan hasil regenerasi secara in vitro dengan baik.

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In vitro seed production can be used as an alternative method in seed multiplication of superior cultivars sources, but the research concerning the use of the growth regulators to support regeneration and encapsulation in this cultivar has never been done. The objective of this experiments is to find out the best combination of TDZ and NAA on medium MS + vitamin from medium B5 in the regeneration of embryonic axis explant and determine the encapsulation method used in this research that can encapsulate soybean cv Rajabasa in vitro. Current research was carried out from May to August 2014 at Tissue Culture Laboratory, Faculty of Agriculture, Universitas Padjadjaran. The explants used in the research are embryonic axis of Rajabasa soybean cultivar. Its experimental design was a Completely Randomized Design (CRD). The used medium was MS and vitamin from medium B5 with the addition of growth regulators TDZ (0 mgL- 1; 0.01 mgL- 1; 0.1 mgL- 1; 1.0 mgL- 1) and NAA (0 mgL- 1; 0.01 mgL- 1; 0.1 mgL- 1; 1.0 mgL- 1). Second stage of encapsulation in explants regenerated using Na-Alginate 4% + CaCl2.2H2O 100 mM. The best treatment was obtained on combination of TDZ 0,01 mgL-1 + NAA 0 mgL-1, but the encapsulation stage in this research has not been able to encapsulates regenerated explants in vitro. "