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Abstrak :
This major reference work is a one-shot knowledge base on electroporation and the use of pulsed electric fields of high intensity and their use in biology, medicine, biotechnology, and food and environmental technologies. The Handbook offers a widespread and well-structured compilation of chapters ranging from the foundations to applications in industry and hospital. It is edited and written by most prominent researchers in the field. With regular updates and growing in its volume it is suitable for academic readers and researchers regardless of their disciplinary expertise, and will also be accessible to students and serious general readers. The authors of chapters have established scholarly credentials and come from a wide range of disciplines. This is crucially important in a highly interdisciplinary field of electroporation and the use of pulsed electric fields of high intensity and its applications in different fields from medicine, biology, food processing, agriculture, process engineering, energy and environment.

Abstrak :
Telah dilakukan penelitian yang bertujuan untuk melakukan transformasi gen OsERA1 ke kalus padi cv. Taipei 309 menggunakan Agrobacterium tumefaciens. Gen OsERA1 adalah gen yang berperan dalam meningkatkan sensitifitas dari sel penjaga pada stomata terhadap asam absisat. Transformasi gen OsERA1 dilakukan menggunakan Agrobacterium tumefaciens strain LBA4404 yang membawa plasmid rekombinan pCAMBIA1301-OsERA1. Gen OsERA1 telah dikloning sebelumnya pada vektor pengklonaan pGEM-T Easy. Gen dipotong dengan enzim restriksi BamHI dan SalI. Gen diligasikan dengan pCAMBIA 1301 dan ditransformasikan ke dalam Escherichia coli DH5α dihasilkan vektor rekombinan pCAMBIA-ERA1. Plasmid vektor rekombinan pCAMBIA-ERA1 belum berhasil ditransformasi ke dalam Agrobacterium tumefaciens dengan elektroporasi tetapi berhasil dilakukan dengan particle bombardment. ......Research about transformation of OsERA1 gene into rice calli cv. Taipei 309 using Agrobacterium tumefaciens had been done. OsERA1 gene is a gene that has response to enhance sensitivity of guard cell to absicid acid. Transfer of OsERA1gene into calli was carried out using Agrobacterium tumefaciens strain LBA4404 harboring recombinant plasmid pCAMBIA1301-OsERA1. OsERA1 gene had been cloned previously in the pGEM-T Easy cloning vector and for inserting into pCAMBIA1301, the gene was digested from cloning vector using restriction enzymes BamHI and SalI. Furthermore, the gene was ligated into vector pCAMBIA 1301 and transformed into Escherichia coli DH5α in order to obtain recombinant plasmid pCAMBIA-OsERA1. The recombinant plasmid pCAMBIAOsERA1has not sucessfully transformed with electroporation into Agrobacterium tumefaciens yet, but it can be transformed by particle bombardment method.

Abstrak :
The Handbook of Single Cell Technology provides an overview of single cell manipulation, injection, lysis, and dynamics analysis with the aid of various miniaturized devices. The role of single cell analysis in system biology, proteomics, genomics, metabolomics and fluxomics, the applications of single cell analysis for bio-catalysis, metabolic and bioprocess engineering, and the future challenges for single cell analysis given its advantages and limitations are also elaborated. The respective chapters introduce readers to various approaches for single cell analysis. Further, they address the fabrication of different types of bio-micro/nano devices in the context of cutting-edge analysis and screening for e.g. cancers, HIV etc., which is beneficial for society at large. This handbook is intended for academic researchers, undergraduate and graduate students in the fields of Biomedical Engineering, Bio-nanoengineering, and Bio-micro/nano Fabrication. It can be used for courses on Bio-MEMS/Bio-NEMS, Biomicrofluidics, Biomicrofabrications, Micro/Nanofluidics, Biophysics, Single Cell Analysis, Bionanotechnology, Drug Delivery Systems and Biomedical Microdevices. Bringing together contributions from respected experts, it will also benefit researchers and practitioners in the biotechnology industry, where diseases analysis, diagnosis and drug screening continue to grow in importance.