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Abstrak :
Virus Ebola adalah salah satu penyakit paling mematikan di dunia, dengan hampir 29.000 kasus melaporkan dan membunuh 11.000 dari mereka, tetapi tidak ada perawatan atau vaksin yang dapat melawan penyakit ini secara efektif. Penyakit ini disebabkan oleh virus ebola (EBOV), anggota utama dari keluarga Filoviridae. Siklus hidup virus ini telah dioperasikan oleh beberapa mayor protein, salah satunya adalah protein HSP70, yang telah dikenal penting peran dalam transkripsi dan replikasi EBOV. Karena itu, targetkan protein HSP70 dapat menjadi solusi untuk mengobati penyakit patogen ini. Dalam penelitian ini, skrining virtual Produk alami Indonesia dilakukan sebagai inhibitor HSP70 EBOV. Molekuler simulasi docking dilakukan untuk menguji interaksi dan afinitas ligan obligasi dengan protein HSP70 EBOV; simulasi ini dilakukan dengan menggunakan MOE 2014.09 perangkat lunak. Hasil yang diperoleh menunjukkan bahwa penghambatan HSP70 berkurang secara signifikan Replikasi EBOV dengan menggunakan ligan senyawa bahan alami Indonesia. Itu nilai bioavailabilitas diperoleh sebesar 0,56. Ini menunjukkan bahwa obat tersebut dapat digunakan secara oral.

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The Ebola virus is one of the deadliest diseases in the world, with nearly 29,000 cases reporting and killing 11,000 of them, but there is no treatment or vaccine that can fight this disease effectively. This disease is caused by the Ebola virus (EBOV), a major member of the Filoviridae family. The life cycle of this virus has been operated by several major proteins, one of which is the HSP70 protein, which has been recognized for an important role in the transcription and replication of EBOV. Therefore, targeting the HSP70 protein can be a solution to treat this pathogenic disease. In this study, virtual screening of Indonesian natural products was carried out as an EBP HSP70 inhibitor. Molecular docking simulation was carried out to test the interaction and affinity of bond ligands with the EBP HSP70 protein; This simulation was carried out using MOE 2014.09 software. The results obtained showed that HSP70 inhibition was significantly reduced by EBOV replication using ligands of Indonesian natural compounds. The bioavailability value was obtained at 0.56. This shows that the drug can be used orally.

Abstrak :
Although Ebola and similar hemorrhagic fevers have occurred in the past, both the numbers and geographic spread of the 2014-15 West African Ebola epidemic were unprecedented. Ebola and the associated risks drove an improvised, sometimes ineffective, response from political and medical authorities. Fear, rather than rational planning, drove many decisions made at population and leadership levels. Institutions, practices, economies, and governments were all deeply affected by the demands engendered by this emergency. Ultimately, the epidemic revealed serious fault lines at all levels in the theories and practices of global public health. Doctors Without Borders/Medecins sans Frontieres (MSF), as the major provider of medical care to the afflicted, was deeply entangled in many of these issues. From difficult choices made for the care of individual patients to the impact of Ebola on entire health systems, the common thread in each chapter is how fear influenced the political and medical response. Using materials from the MSF archives, this book explores this theme in ten chapters and four eyewitness vignettes. The book examines the epidemic from the perspectives of a wide range of actors from distinct sectors, including a bioethicist, a political scientist, a historian, clinical doctors, policymakers, and anthropologists.

Abstrak :
Ebola adalah virus penyebab fatal hemoragik pada manusia dan primata non-manusia. Virus ebola memiliki lima spesies dan yang paling mematikan ialah virus ebola Zaire. Genom virus ebola mengkodekan tujuh protein yang penting bagi siklus hidupnya dan penelitian ini dilakukan untuk menghambat VP24 virus ebola Zaire. VP24 memiliki potensi sebagai target obat karena memiliki peran penting dalam replikasi virus ebola dan penghambatan interferon sel inang. Dalam penelitian ini, senyawa terpenoid digunakan untuk menghambat VP24 Zaire secara komputasi dengan menggunakan fitur farmakofor. Terdapat 55.979 senyawa terpenoid diperoleh dari pangkalan data Pubchem kemudian ligan disaring berdasarkan sifat toksisitas melalui Osiris DataWarrior dan terdapat 3.353 ligan yang memiliki sifat toksisitas yang menguntungkan. Selanjutnya fitur farmakofor digunakan untuk menyaring ligan dan 1.375 ligan memiliki struktur yang sesuai dengan fitur farmakofor. Kemudian ligan dilakukan simulasi penambatan molekul dengan target protein dan terdapat 10 ligan terbaik berdasarkan sifat molekul dan interaksi molekuler. Selanjutnya, semua ligan dianalisa sifat farmakologisnya melalui Osiris DataWarrior, Toxtree, admetSAR, SwissADME, dan pkCSM. Akhirnya, terdapat tiga ligan terbaik yaitu Taxumairol V, Acrivastine, dan 3-O-acetyluncaric acid. Dalam penelitian ini, Taxumairol V adalah ligan terbaik untuk menghambat VP24 virus ebola Zaire karena ligan memiliki sifat molekul, interaksi molekuler, dan sifat farmakologis yang baik sebagai kandidat obat.

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Ebola is a pathogenic virus which causes fatal hemorrhagic fever in humans and non-human primates. Ebolavirus has five species, and there is the most pathogenic of ebolavirus namely Zaire ebolavirus. Ebola virus genome encodes seven protein which important for the ebolavirus life cycle and in this research was conducted to inhibit VP24 Zaire ebolavirus. VP24 has potential as a drug target because VP24 has an essential role in replication and interferon inhibition. In this research, 55,979 terpenoid compounds were obtained from PubChem database then the ligands were screened based on toxicity properties through Osiris DataWarrior, and there were 3,353 ligands which have beneficial toxicity properties. Afterward, the pharmacophore features were used to screen all ligands and 1,375 ligands have suitable structures according to the pharmacophore features. All ligands were docked with the protein target, and there were ten best ligands based on the molecular properties and interactions. Furthermore all ligands were investigated their pharmacological properties through Osiris DataWarrior, Toxtree, admetSAR, SwissADME, and pkCSM. Finally, there were three best ligands namely, Taxumairol V, Acrivastine, and 3-O-acetyluncaric acid. In this study, Taxumairol V was the best ligand to inhibit VP24 Zaire ebolavirus because the ligand has good molecular interactions, molecular and pharmacological properties.

Abstrak :
ABSTRAK
Wabah penyakit Ebola di Afrika Barat yang meledak pada tahun 2014 menyebabkan dampak kerugian yang besar pada bidang ekonomi, politik dan sosial sehingga mendorong PBB mengeluarkan Resolusi 2177. Disusul pada tahun 2015, terjadi peningkatan penularan penyakit MERS dari Timur Tengah sampai ke wilayah Korea Selatan dan ASEAN. Ebola dan MERS merupakan jenis penyakit Zoonosis, yaitu penyakit (infeksi) yang dapat menular dari hewan ke manusia dan sebaliknya. Untuk mencegah masuknya penyakit Zonosis dari luar negeri ke Indonesia diperlukan kondisi Biosecurity disertai dengan Sistem Kesehatan Nasional yang kuat. Hal tesebut disebabkan karena perang Asimetrik (Asymetric Warfare) dewasa ini tidak hanya melibatkan senjata konvensional, namun juga wabah penyakit dan penguasaan ekonomi. Kebarhasilan penanganan penyakit Zoonosis juga dipengaruhi oleh kemampuan industri vaksin nasional suatu negara. Indonesia merupakan salah satu negara dengan potensi penyakit zoonosis (terutama tropical disease) yang besar. Kemandirian suatu negara untuk membuat vaksin sendiri dari penyakit-penyakit zoonosis yang mewabah akan meningkatkan kemampuan Biosecurity negara tersebut, sehingga tidak terlalu bergantung dan dapat ?disetir? oleh industri vaksin global. Tesis ini membahas mengenai penilaian terhadap ancaman dan peluang Biosecurity di Indonesia, yang salah satunya melibatkan kemampuan industri vaksin nasional. Melalui faktor-faktor yang berpengaruh terhadap obyek penelitian, penelitian ini memberikan penjelasan (eksplanasi) terhadap kondisi kemampuan Biosecurity Nasional. Penilaian terhadap kondisi Biosecurity Nasional ini diharapkan dapat digunakan sebagai rekomendasi bagi stakeholder terkait untuk melihat dan mengevaluasi bagaimana peluang dan ancaman pengembangan industri vaksin di masa mendatang

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ABSTRACT
Ebola Virus Disease (EVD) outbreaks in West Africa that was exploded in 2014 causing huge impact on economic, political and social. The spread of Ebola and this impact encouraged the United Nations to adopt Resolution 2177. One year after Ebola outbreak, in 2015, the case number of another zoonotic disease like Middle East Respiratory Syndrome (MERS) have increased, and transmitted to South Korea and also to ASEAN region. Ebola and MERS are zoonotic disease that can be transmitted from animals to human. Indonesia needs strong Biosecurity to prevent zoonotic disease from another country or region. Biosecurity condition is accompanied by a strong National Health System. Asymmetric warfare involves not only conventional weapons, but also the uses of diseases and control of the economy. The ability of handling zoonotic diseases in a country is also affected by the condition of their national vaccine industry. Indonesia is one country with the potential for zoonotic diseases (called tropical disease country). The independence of a country to make its own vaccine will increase the ability of Biosecurity of the country, so it is not too dependent and can be "driven" by the global vaccine industry. This thesis discusses the assessment of the threats and opportunities for Biosecurity in Indonesia, one of which involves the ability of national vaccine industry. This research also provides an explanation of the condition of the ability of the National Biosecurity. An assessment of the condition of the National Biosecurity is expected to be used as recommendations to the relevant stakeholders to see and evaluate how the opportunities and threats in Indonesian Biosecurity in the future.

Abstrak :
ABSTRAK
Virus Ebola (EBOV) merupakan penyebab ebola hemorrhagic fever yang berakibat fatal bagi manusia. Protein Niemann Pick C1 (NPC1) merupakan protein pada organisme inang yang memiliki peranan yang penting dalam proses masuknya EBOV ke dalam sel organisme inangya. Protein ini berikatan dengan primed-glycoprotein (GPcI) yang merupakan protein dari EBOV yang menjadi jalur utama virus menginfeksi. Pada penelitian ini, senyawa peptida digunakan sebagai kandidat inhibitor dari protein NPC1. Sebanyak 12,863 senyawa peptida yang melalui proses virtual screening, rigid docking, dan flexible docking yang kemudian dilakukan penapisan berdasarkan nilai Root Mean Square Deviation (RMSD) dan ΔGbinding, serta dilakukan analisis interaksi protein-ligan dan uji sifat farmakologi. Dari hasil keseluruhan proses tersebut diperoleh tiga senyawa peptida, yaitu Alarelin, Neurokinin beta, dan Callitachykinin I, yang kemudian menjalani simulasi dinamika molekul. Selanjutnya, ketiga senyawa peptida ini dikonjugasi dengan peptida protein HIV-1 tat yang berfungsi sebagai carrier peptida sehingga ketiga ligan tersebut dapat terakumulasi di dalam endosom. Selanjutnya, untuk ketiga senyawa peptida yang telah dikonjugasi ini (C-Peptida) dilakukan proses penambatan molekul kembali. Interaksi ketiga ligan C-peptida ini menunjukkan konformasi yang hampir sama dengan interaksi peptida sebelum dikonjugasi. Dari hal ini dapat dikatakan bahwa sebelum dan setelah dikonjugasi memberi efek inhibisi yang sama. Dari berbagai proses yang dilakukan dapat disimpulkan bahwa C-Callitachykinin I merupakan peptida terbaik yang dapat dijadikan kandidat obat untuk menginhibisi NPC1.

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ABSTRACT
Ebola virus (EBOV) is a cause of ebola hemorrhagic fever which is generate fatal disease to human. Niemann Pick C1 (NPC1) protein is a protein in the host cell that has important function in the process of EBOV entry into the cell. This protein binds to primed-glycoprotein (GPcI) which is a protein from EBOV which is the main pathway for infecting viruses. In this study, peptide compounds were used as candidate inhibitors of NPC1 proteins. A total of 12,863 peptide compounds through the process of virtual screening, rigid docking, and flexible docking were then screened based on the value of Root Mean Square Deviation (RMSD) and ΔGbinding, and analysis of protein-ligand interactions and testing of pharmacological properties. From the results of the whole process three peptide compounds were obtained, namely Alarelin, Beta Neurokinin, and Callitachykinin I, which then underwent molecular dynamics simulations. Furthermore, these three peptide compounds are conjugated with the HIV-1 tat protein peptide which functions as a carrier peptide so that the three ligands can accumulate in the endosome. Furthermore, for the three conjugated peptide compounds (C-Peptides) the molecular tethering process is carried out again. The interaction of the three C-peptide ligands shows conformation that is almost the same as the peptide interaction before conjugation. From this it can be said that before and after conjugation had the same inhibitory effect. From various processes carried out it can be concluded that C-Callitachykinin I is the best peptide that can be used as a drug candidate to inhibit NPC1

Abstrak :
The outbreak of Ebola virus disease in West Africa shocked the world as the disease spread rapidly from its origin to neighboring countries, Europe, and North America while the systems in place to handle such an epidemic failed. The United Nations, the World Health Organization, and major international humanitarian organizations scrambled to respond as thousands died and infections spiraled out of control. All are now contemplating: What went wrong, and how do we stop it from happening again? Global Management of Infectious Disease After Ebola is the first and most comprehensive volume to address these questions. It brings together the analyses and retrospectives of diplomats, scholars, and advocates studying from afar, as well as those of physicians and front-line responders who witnessed the epidemic sweep through already poor, devastated countries as their nascent health systems collapsed. The volume assesses not only the global response to Ebola but also current and emerging infectious disease threats, changes in the global system to handle them, and the critical ethics and human rights issues that will shape the next episode in the perpetual struggle against infectious disease.