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"World Health Organization (WHO) tahun 2003 menyatakan bahwa prevalensi karies gigi anak usia 3–6 tahun sebesar 60-90%. Perilaku ibu dalam memelihara kesehatan gigi mempengaruhi kesehatan gigi anak. Tujuan pembuatan skripsi ini adalah untuk mengetahui peran video terhadap peningkatan perilaku ibu dalam menyikat gigi anak. Penelitian dilakukan dengan mengedukasi ibu dengan video, ibu mengisi tabel evaluasi menyikat gigi, pemeriksaan gigi anak, dan mengevaluasi hasil edukasi. Terjadi peningkatan bermakna (p<0,05) perilaku ibu dalam menyikat gigi anak (57,8 %), penurunan bermakna (p<0,05) indeks plak (75,6 %) dan skor kematangan plak (57,8%). Video berpotensi efektif untuk meningkatkan perilaku ibu dalam menyikat gigi anak.

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Survey by WHO in 2003, prevalence of early childhood caries is 60-90%. Mother's behavior in maintaining dental health can influence children's dental health. This study aims to know the role of video in improving mother's behavior in tooth brushing her children. In this research, there's an education using video for the mothers, mothers fill the evaluation's table, examination of children's teeth, evaluating after the intervention. There's a significant improvement (p<0,05) of mother's behavior (57,8%), a significant reduction (p<0,05) of plaque index (75,6%) and plaque maturity score (57,8%). Video is potentially effective to improve mother's behavior in tooth brushing her children."

"Latar belakang: Karies gigi merupakan masalah kesehatan gigi dan mulut dengan prevalensi dan keparahan yang tinggi dan menyerang seluruh negara termasuk Indonesia. Karies gigi juga dapat terjadi sejak dini pada anak prasekolah dan dapat mempengaruhi kualitas hidup anak tersebut. Tujuan: Mengetahui hubungan antara tingkat karies anak prasekolah dengan kualitas hidup terkait kesehatan mulut di Kelurahan Grogol Utara, Kebayoran Lama, Jakarta Selatan. Metode: Desain cross-sectional secara analitik observasional. Sebanyak 200 anak prasekolah umur 3-5 tahun dipilih dengan teknik purposive dan dilakukan pemeriksaan gigi dan mulut untuk menilai skor deft, defs serta menentukan status karies oleh 2 dokter gigi yang sudah dilatih. Kualitas hidup dinilai melalui wawancara kuesioner ECOHIS ke 165 ibu anak. Hasil: Prevalensi karies pada 165 anak umur 3-5 tahun adalah sebesar 83 dengan nilai defs 14.8 SD17,6 dan deft 6,2 SD5,2. Pola distribusi karies pada anak usia 3-5 tahun paling banyak ditemukan di gigi insisif sentral dan lateral maksilla dan pola karies hampir simetris antar rahang. Berdasarkan hasil uji Cronbach - 0,868, test-retest 0,968 , dan perbandingan r-hitung tiap item dengan r-tabel, kuesioner ECOHIS versi Bahasa Indonesia reliabel dan valid. Frekuensi item kuesioner ECOHIS ditemukan terbesar di item nyeri mulut pada gigi/mulut pada anak 38,2 serta rasa bersalah pada keluarga 30,3. Uji dilakukan dengan menggunakan uji deskriptif, korelasi Spearman, uji Kruskall-Wallis dan uji Mann-Whitney U. Nilai korelasi tertinggi r=0,4 ditemukan pada item nyeri mulut dan gigi pada anak dan rasa bersalah pada keluarga dengan skor deft dan skor d pada defs anak. Hubungan status karies dan kualitas hidup baik yang berdampak ke anak dan keluarga ditemukan bermakna.

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Background: Dental caries has become a major global oral health problem with high prevalence and severity, including Indonesia. Dental caries can develop early in preschool children and will affect their quality of life.

Aim: To assess the relationship between the severity of caries in preschool children and their oral health related quality of life in Grogol Utara, Kebayoran Lama, Jakarta Selatan.

Method" Analytic observational with cross sectional design. A purposive sample of 200 children aged 3 5 years underwent a clinical oral examination to assess their deft and defs score and to determine their caries status by 2 trained dentists. Quality of life is assessed using ECOHIS by interviewing a total of 165 mother's child.

Results: The prevalence of ECC in 165 children aged 3 5 years old is 83 with overall defs score 14.83 SD 17.6 and deft score 6.2 SD 5.2. The tooth distribution pattern of caries in 3 5 years old children mostly affect central and lateral maxillary incisors. Moreover, the caries pattern was almost symmetrical across the arches. The Cronbach 0,868 test retest 0,968 and r count for each item comparison with r table shows that Indonesian version of ECOHIS is a reliable and valid instrument. The most frequent items reported are pain in teeth, mouth and jaw in child 38.2 and feeling guilty in family 30.3. Descriptive analysis, Spearman correlation, Kruskall Wallis test, and Mann Whitney U test were used. The highest correlation r 0.4 were found in item is pain in teeth, mouth and jaw in child and feeling guilty in family with deft score and d score in defs. There was significant difference between caries status and OHRQoL in terms of impact on both child and family p"

"Karies gigi adalah salah satu penyakit menular kronis yang palingumum pada anak-anak usia prasekolah. Bentuk agresif karies gigi pada gigi sulung anakdi bawah, sampai dengan usia 71 bulan disebut dengan Early Childhood Caries (ECC).Indonesia melaporkan prevalensi dan keparahan ECC yang tinggi hingga mencapai angka90%. DKI Jakarta memiliki prevalensi sebesar 81,2%. Faktor risiko utama ECC yaituhost (gigi dan saliva), mikroorganisme kariogenik, dan karbohidrat (substrat).Mikroorganisme kariogenik yang berperan yaitu Streptococcus mutans. Penelitian diJakarta pada anak usia 3-5 tahun yang memiliki karies, menunjukkan bahwa serotipe fmerupakan jenis yang paling banyak di temukan (85,5%), diikuti dengan serotipe c(74,2%), serotipe e (22,6%) dan serotipe d (19,4%). Penelitian lainnya di Jakartamenemukan bahwa kombinasi serotipe c dan f lebih tinggi pada anak yang memilikikaries. Streptococcus mutans serotipe c dan f berperan dalam patogenesis karies gigi, haltersebut sesuai dengan tingginya tingkat karies gigi di Indonesia. Salah satu faktor hostyaitu saliva, merupakan cairan tubuh yang kompleks yang terdiri dari unsur-unsurorganik dan anorganik yang penting untuk kesehatan rongga mulut. Komposisi proteinsaliva seperti Lactoferrin sangat penting karena memiliki kemampuan antibakteri sertaberperan dalam sistem imun bawaan dan adaptif. Tujuan penelitian ini adalah untukmenganalisis kadar Lactoferrin saliva antara anak Early Childhood Caries (ECC) danbebas karies pada usia 3-5 tahun. Metode penelitian: Desain penelitian ini adalah potonglintang analitik secara laboratorik. Penelitian ini dilakukan pada 14 anak dengan ECCdan 14 anak bebas karies. Saliva didapat dari seluruh subjek dan kadar LF diukurmenggunakan metode ELISA sandwich. Hasil: Analisis data menggunakan uji MannWhitney U menunjukkan terdapat perbedaan bermakna antara kadar Lactoferrin anakECC dan anak bebas karies dengan nilai p=0,006 (p<0,05). Kesimpulan: KadarLactoferrin saliva anak Early Childhood Caries (ECC) lebih tinggi dari anak bebas kariesyang menunjukkan bahwa Lactoferrin dapat menjadi indikator peningkatan risiko EarlyChildhood Caries (ECC).Backgrounds: Dental caries is one of the most common chronic infectious diseases inpreschool children. The aggressive form of dental caries in the primary teeth of childrenunder and up to the age of 71 months is called Early Childhood Caries (ECC). Indonesiareports a high prevalence and severity of ECC (90%). DKI Jakarta has a prevalence81.2%. The main risk factors for ECC are hosts (teeth and saliva), cariogenicmicroorganisms, and carbohydrates (substrates). The cariogenic microorganisms thatplay a role are Streptococcus mutans. Research in Jakarta on children aged 3-5 years whohad caries, showed that serotype f was the most common type (85.5%), followed byserotype c (74.2%), serotype e (22.6%) and serotype d (19.4%). Another study in Jakartafound that the combination of serotypes c and f was higher in children with caries.Streptococcus mutans serotypes c and f play a role in the pathogenesis of dental caries,which is consistent with the high level of dental caries in Indonesia. One of host factor,saliva, is a complex body fluid consist of organic and inorganic elements that areimportant for oral health. Salivary protein such as Lactoferrin is very important becauseit has antibacterial ability and plays an important role in innate and adaptive immunesystem. The purpose of this study is to analyze Lactoferrin levels between EarlyChildhood Caries (ECC) and caries-free children aged 3-5 years. Methods: The designof this study is cross-sectional analytical laboratory. This study was conducted on 14children with ECC and 14 caries-free children. Saliva were taken from all subjects and theLactoferrin levels were measured using ELISA sandwich method. Results: Data analysisusing the Mann Whitney U test showed that there were significant differences betweenthe levels of salivary Lactoferrin in children with ECC and caries-free children with pvalue0,006 (p<0,05). Conclusion: Salivary Lactoferrin levels in Early ChildhoodCaries (ECC) were higher than caries-free children which indicate that Lactoferrin canbe an indicator of an increased risk of Early Childhood Caries (ECC)."

"Dental caries is a multifactorial disease that can affect all levels of society. There are several factors that take part ini caries initiation. Caries risk factors in children are related to age, gender, use of bottles, tooth brushing frequency, the role of the mother, and parental education. The purpose of this studywas to find out factors related to the cause of early childhood caries experienced by preschool children at PAUD Kenanga 17 Kelurahan Penjaringan, Kecamatan Penjaringan, North Jakarta. An observational analytical methods with cross sectional approach was used in this study. Subject in this study consisted of 20 children as a total sample of the population. Data collection was carried out using questionnaires and def-t index was employed for dental examination. Data processing and statistical analysis using chi-square test were applied with a significance value of p <0.05. The results showed significant correlation between age (p value 0.001) and gander (p value 0.047) andearly childhood caries.however, no correlation was observed betwen the use of bottle (p value = 0.909), frequency of tooth brushing (p value 1.00), role of mother (p value 1.00), and i parental education (p value = 0.798) and early childhood caries."

"Latar belakang: Early Childhood Caries (ECC) terjadi apabila terdapat satu atau lebih gigi karies, gigi yang hilang akibat karies atau gigi yang telah ditambal pada anak usia dibawah 71 bulan. Dalam dua dekade terakhir berbagai studi menunjukkan Candida albicans (C. albicans) juga merupakan faktor utama penyebab karies. Tindakan pencegahan ECC dapat dilakukan dengan menyikat gigi menggunakan pasta gigi secara rutin. Salah satu bahan herbal yang telah terbukti memiliki efek antimikroba adalah Virgin Coconut Oil (VCO). Kandungan asam lemak pada VCO menyebabkan VCO memiliki efek antifungal, antibakteri dan antivirus Metode Penelitian: Penelitian ini menguji VCO sediaan pasta gigi konsentrasi 8% dan 80% terhadap viabilitas biofilm C. albicans pasien ECC. Biofilm dibentuk pada 96-microwell plate. Setelah inkubasi 24 jam, plat dimasukkan ke dalam microplate reader untuk mendapatkan nilai viabilitas biofilm diukur menggunakan perhitungan optical density (OD). Hasil: Analisis data menggunakan uji t-test tidak berpasangan menunjukkan nilai viabilitas biofilm C. albicans setelah pemberian VCO sediaan pasta gigi konsentrasi 8% berbeda tidak bermakna dengan kontrol negatif (tanpa bahan uji). Sedangkan nilai viabilitas biofilm C. albicans setelah pemberian VCO sediaan pasta gigi konsentrasi 80% menunjukkan nilai viabilitas biofilm C. albicans berbeda bermakna dengan kontrol negatif. Kesimpulan: Penelitian ini menunjukkan viabilitas biofilm C. albicans setelah pemberian VCO sediaan pasta gigi konsentrasi 8% meningkat. Sebaliknya, VCO sediaan pasta gigi konsentrasi 80% menurunkan viabilitas C. albicans.

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Background: Early Childhood Caries (ECC) is defined as a child 71 months of age or younger with the presence of one or more decayed teeth, missing (due to caries) or filled teeth surface. In the past two decades, various studies have shown Candida albicans (C. albicans) also the main etiology of dental caries. ECC prevention can be carried out by brushing teeth regularly using toothpaste. VCO is one of natural product that has been proven to have antimicrobial effect. The fatty acid content in VCO causes VCO to exhibit antifungal, antibacterial and antiviral effect. Methods: This study tested VCO 8% and 80% toothpaste against C. albicans biofilm viability. Biofilm was formed on 96-microwell plate. After 24 hours incubation, plate was inserted into microplate reader to obtain biofilm viability value measured using optical density (OD). Results: Data analysis using independent t-test showed C. albicans biofilm viability in VCO 8% toothpaste group was not significantly different from negative control. However, C. albicans biofilm viability in VCO 80% toothpaste was significantly different from negative control. Conclusion: This study showed increasing C. albicans biofilm viability after VCO 8% toothpaste exposure. In contrast, VCO 80% toothpaste decreased C. albicans biofilm viability."

"Sebelum adanya penggunaan susu formula sebagai pengganti ASI, gigi berlubang pada bayi jarang ditemukan.2,19 Dilaporkan pada anak riwayat ASI Eksklusif, karies jarang ditemukan karena mendapat komponen imunitas khususnyaIgA yang dapat memperlambat pertumbuhan bakteri S.mutans.2,11 Pada anak riwayat susu formula komponen imunitas belum diketahui. Penelitian ini bertujuan untuk menganalisis perbedaan kadar sIgA saliva anak ECC riwayat ASI eksklusif dan susu formula. Penelitian ini dilakukan pada 34 anak ECC usia 18-48 bulan yang memiliki skor deft >1, dengan 17 subjek riwayat ASI eksklusif dan 17 subjek riwayat susu formula. Seluruh subjek dilakukan pemeriksaan skor deft, dan dilakukan pengukuran sampel saliva dengan ELISA. Rerata skor deft anak ECC kelompok riwayat ASI eksklusif lebih rendah dibanding susu formula. Terdapat perbedaan bermakna rerata kadar sIgA saliva anak ECC antara riwayat ASI eksklusif dan susu formula (p=0,004).

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Time before formula feeding has been found, baby tooth decay is definitely rare.2,19 Studies reported, children with exclusive breastfeeding have low caries as they have immunity component, specifically IgA, which may exhibits colony of S.mutans.2,11Meanwhile, immunity component of children with formula feeding is barely unknown. This study aimed to analyze the difference of quantity salivary sIgA Early Childhood Caries (ECC) children between exclusive breastfeeding and formula feeding history. Saliva samples were collected from 34 ECC children aged 18-48 months who have deft score >1, both exclusive brestfeeding and formula feeding history group are 17 subjects each. Deft score were examined, and quantity of salivary sIgA were assesed by ELISA. Deft score mean of exclusive breastfeeding history group is lower than formula feeding history group. There is a significant difference quantity salivary sIgA ECC children between exclusive breastfeeding and formula feeding history (p=0,004)."

"ABSTRAKPatogenesis ECC dipengaruhi oleh salah satu faktor virulensi Streptococcus mutans yang berasal dari protein S.mutans. Tujuan : Menganalisis perbedaan profil protein S.mutans diisolasi dari permukaan lidah pasien ECC dan bebas karies. Metode : Profil protein S.mutans diisolasi dari permukaan lidah diperoleh melalui metode SDS PAGE dan dibaca melalui pita protein yang terlihat pada gel poliakrilamida. Hasil : Pita protein terlihat pada gel poliakrilamida. Terlihat perbedaan frekuensi ekspresi protein S.mutans pada 13 kDa, 29 kDa, 39 kDa, 41,3 kDa, 74 kDa dan 94,5 kDa pasien ECC dan bebas karies. Kesimpulan : Terdapat perbedaan profil protein S.mutans yang diisolasi dari permukaan lidah pasien ECC dan bebas karies.

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ABSTRACTPathogenesis of ECC is influenced by one of virulence factors from protein S.mutans. Objective To analyze the difference of S.mutans protein profiling which is isolated from tongue surface in ECC dan free caries subjects. Method Protein Profiling of S.mutans isolated from tongue surface was obtained from SDS PAGE method. It was read by protein band which expressed on polyacrylamide gel. Result Protein band was present on polyacrylamide gel. This study found the different frequencies in protein expression of S.mutans 13 kDa, 29 kDa, 39 kDa, 41,3 kDa, 74 kDa dan 94,5 kDa in ECC and free caries subjects. Conclusion There is difference of S.mutans protein profiling isolated from tongue surface in ECC and free caries subjects."

"ABSTRAKEarly Childhood Caries ECC merupakan penyakit infeksi kronis pada gigi anak-anak usia 3 ndash; 5 tahun, yang dipengaruhi oleh berbagai faktor, salah satunya adalah saliva. Saliva memiliki viskositas dan komponen salah satunya adalah protein yang berperan penting dalam mempertahankan rongga mulut dari patogen penyebab ECC. Tujuan: Menganalisis profil protein dan viskositas saliva pada pasien ECC dan bebas karies. Metode: Viskositas saliva dinilai secara visual dan profil protein saliva dianalisis menggunakan metode SDS PAGE. Hasil: Ditemukan 5 profil protein dengan gambaran yang paling jelas dengan berat molekul 15 kDa, 25 kDa, 60 kDa, 65 kDa dan 95 kDa pada pasien ECC dengan frekuensi kemunculan secara berurutan sebesar 8, 3, 16, 6, 2 dan pada bebas karies sebesar 14, 5, 16, 16, 9. Viskositas saliva pada pasien ECC tinggi dan pada bebas karies rendah. Kesimpulan: Pada pasien ECC ditemukan frekuensi kemunculan profil protein 15 kDa, 25 kDa, 65 kDa dan 95 kDa yang lebih sedikit dibandingkan dengan bebas karies. Sedangkan profil protein 60 kDa ditemukan frekuensi kemunculan yang sama pada pasien ECC dan bebas karies. Viskositas pada pasien ECC lebih tinggi dibandingkan dengan bebas karies. Latar Belakang: Early Childhood Caries ECC merupakan penyakit infeksi kronis pada gigi anak-anak usia 3 ndash; 5 tahun, yang dipengaruhi oleh berbagai faktor, salah satunya adalah saliva. Saliva memiliki viskositas dan komponen salah satunya adalah protein yang berperan penting dalam mempertahankan rongga mulut dari patogen penyebab ECC. Tujuan: Menganalisis profil protein dan viskositas saliva pada pasien ECC dan bebas karies. Metode: Viskositas saliva dinilai secara visual dan profil protein saliva dianalisis menggunakan metode SDS PAGE. Hasil: Ditemukan 5 profil protein dengan gambaran yang paling jelas dengan berat molekul 15 kDa, 25 kDa, 60 kDa, 65 kDa dan 95 kDa pada pasien ECC dengan frekuensi kemunculan secara berurutan sebesar 8, 3, 16, 6, 2 dan pada bebas karies sebesar 14, 5, 16, 16, 9. Viskositas saliva pada pasien ECC tinggi dan pada bebas karies rendah. Kesimpulan: Pada pasien ECC ditemukan frekuensi kemunculan profil protein 15 kDa, 25 kDa, 65 kDa dan 95 kDa yang lebih sedikit dibandingkan dengan bebas karies. Sedangkan profil protein 60 kDa ditemukan frekuensi kemunculan yang sama pada pasien ECC dan bebas karies. Viskositas pada pasien ECC lebih tinggi dibandingkan dengan bebas karies.

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ABSTRACTEarly Childhood Caries ECC is a chronic infection disease of dental on children with 3 ndash 5 years old, that are caused by many factors, one of which is saliva. Saliva has viscosity, and component specifically protein, that play an important role in maintaining oral cavity from pathogen causing ECC. Objective To analyze protein profile and salivary viscosity from ECC and caries free subjects. Method Salivary viscosity was assessed visually and salivary protein profile were analyzed using SDS PAGE. Result 5 most prominent protein profiles with molecular mass 15 kDa, 25 kDa, 60 kDa, 65 kDa and 95 kDa were found in ECC patient with frequency of occurrences in order 8, 3, 16, 6, 2 and in caries free subject 14, 5, 16, 16, 9. Salivary viscosity on ECC patient was high and on caries free patient was low. Conclussion On ECC patient, frequency of occurrences of salivary protein profile 15 kDa, 25 kDa, 65 kDa and 95 kDa were found less than on caries free subject. Meanwhile, protein profile 60 kDa has the same frequency of occurrence on ECC and caries free subject. Viscosity on ECC patient was higher than caries free subject. "

"ABSTRAKPatogenesis ECC disebabkan sifat virulensi dari protein-protein yang menyusun sel Streptococcus mutans. Tujuan: Mengetahui perbedaan profil protein S. mutans isolat saliva pasien ECC. Metode: Profil protein S. mutans berupa pita protein yang terlihat pada gel poliakrilamida diperoleh melalui metode SDS PAGE. Hasil: Profil protein S. mutans diperoleh secara kualitatif melalui interpretasi pita-pita protein yang merepresentasikan berat molekul 13 kDa, 29 kDa, 39 kDa, 41,3 kDa, 74 kDa, dan 94,5 kDa dengan perbedaan frekuensi ekspresi protein pada pasien ECC dan bebas karies. Kesimpulan: Pada pasien ECC dan bebas karies ditemukan adanya perbedaan profil protein dari S. mutans isolat saliva.

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ABSTRACTBackground The pathogenesis of ECC is caused by virulence properties from proteins which construct the cell of Streptococcus mutans. Objective To find out the difference of protein profiling from salivary S. mutans in ECC and free caries. Methods Protein profiling of salivary S. mutans appeared on polyacrilamid gel as protein bands obtained through SDS PAGE. Result The profile obtained through interpretation of protein bands represent molecular mass 13 kDa, 29 kDa, 39 kDa, 41,3 kDa, 74 kDa, and 94,5 kDa which had different frequencies in protein expression from ECC and free caries subjects. Conclusion There is difference in protein profiling of salivary S. mutans both in ECC and free caries subjects. "

"Latar belakang: Early Childhood Caries (ECC) terjadi apabila terdapat satu atau lebih gigi karies, gigi yang hilang akibat karies atau gigi yang telah ditambal pada anak usia dibawah 71 bulan. Salah satu faktor risiko dari ECC adalah mikroorganisme, yaitu Streptococcus mutans yang merupakan bakteri patogen utama dari karies. Salah satu tindakan pencegahan ECC yang efektif adalah menyikat gigi dengan pasta gigi berfluoride, namun penggunaan fluoride dengan dosis tinggi dapat menyebabkan fluorosis. Berdasarkan hal tersebut, telah dilakukan penelitian untuk menemukan pasta gigi berbahan natural. Telah terbukti bahwa Virgin Coconut Oil (VCO) adalah bahan natural yang memiliki efek antimikroba. Metode Penelitian: Penelitian ini menguji VCO pasta gigi 8% dan 80% terhadap viabilitas biofilm S. mutans dari pasien ECC. Nilai viabilitas biofilm didapatkan dengan melihat perbedaan nilai optical density (OD) biofilm S. mutans sebelum dan sesudah diberi bahan uji VCO pasta gigi. Hasil: Analisis data menggunakan uji independen t-Test menunjukkan nilai viabilitas biofilm S. mutans VCO pasta gigi 8% terhadap kontrol negatif berbeda tidak signifikan, namun VCO pasta gigi 80% terhadap kontrol negatif berbeda signifikan. Kesimpulan: Pada penelitian ini, terdapat kenaikan nilai OD biofilm S. mutans setelah pemberian VCO pasta gigi 8%. Sebaliknya, terdapat penurunan nilai OD biofilm S. mutans setelah pemberian VCO pasta gigi 80%. Berdsarkan hal tersebut dapat disimpulkan bahwa VCO pasta gigi konsentrasi 80% memiliki efek antibakteri terhadap viabilitas biofilm S. mutans secara in vitro.

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Background: Early Childhood Caries (ECC) is defined as one or more decayed, missing (due to caries), or filled tooth surfaces in children aged 71 months or younger. Microorganism is one of ECCs risk factor with Streptococcus mutans being the main pathogen of caries. ECC can be prevented effectively using fluoridated toothpaste, but too much fluoride exposure can cause dental fluorosis in children. Studies had been done to create toothpaste with natural active ingredient. One of natural products that has been proven to have antimicrobial activity is Virgin Coconut Oil (VCO). Methods: In this study, VCO toothpaste 8 and 80% concentration was tested against S. mutans biofilm viability. Biofilm viability was measured using the difference between optical density (OD) score before and after VCO toothpastes exposure. Results: The data was analyzed using independent t-Test. S. mutans biofilm viability in VCO toothpaste 8% concentration unsignificantly different from negative control. Meanwhile, VCO toothpaste 80% concentration was significantly different from negative control. Conclusion: In this study, S. mutans biofilm that was exposed with VCO toothpaste 8% concentration showed an increase in the OD score. On the contrary, there was a decrease in the OD score of S. mutans biofilm after being exposed with VCO toothpaste 80% concentration. From the results above, it can be concluded that VCO toothpaste 80% concentration exhibits antibacterial effect against S. mutans biofilm viability in vitro"