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"Keberadaan mikroplastik di perairan dapat mengancam biota di dalamnya. Penelitian dilakukan dengan tujuan menganalisis kelimpahan, bentuk, dan warna mikroplastik pada air, sedimen, insang dan saluran pencernaan ikan sapu-sapu Pterygoplichthys pardalis dari Situ Kenanga dan Situ Mahoni, Kampus Universitas Indonesia, Depok. Pengambilan sampel air dan sedimen dilakukan pada inlet, midlet, dan outlet kedua situ. Sampel air disaring menggunakan plankton net, sampel sedimen diambil menggunakan Ekman grab kemudian dikeringkan menggunakan oven, sampel P. pardalis sebanyak 15 individu diambil menggunakan cast net dari masing-masing situ, kemudian insang dan saluran pencernaannya diisolasi dan didestruksi menggunakan HNO3 65%. Tiap sampel yang diperoleh dilarutkan dengan larutan NaCl jenuh agar terjadi flotasi. Pengamatan dilakukan menggunakan mikroskop cahaya dan Sedgwick Rafter Chamber untuk meletakkan sampel, dengan mengamati bentuk, warna, dan jumlah partikel mikroplastik. Hasil penelitian menunjukkan bahwa bentuk fiber, film, fragmen, granula, serta warna transparan, hitam, biru, dan merah muda ditemukan pada semua sampel. Kelimpahan mikroplastik di Situ Kenanga pada air sebanyak 48,26 ± 23,51 partikel L-1, sedimen 45837,04 ± 36305,97 partikel Kg-1, insang 290,48 ± 154,58 partikel g-1 atau 1156,44 ± 378,69 partikel ind-1, saluran pencernaan 134,37 ± 55,72 partikel g-1 atau 1364,89 ± 339,54 partikel ind-1. Kelimpahan mikroplastik di Situ Mahoni pada air sebanyak 48,63 ± 30,21 partikel L-1, sedimen 36237,04 ± 16702,60 partikel Kg-1, insang 287,23 ± 109,40 partikel g-1 atau 1153,78 ± 324,32 partikel ind-1, saluran pencernaan 123,77 ± 34,35 partikel g-1 atau 1304,44 ± 270,90 partikel ind-1. Tidak terdapat perbedaan signifikan antara kelimpahan mikroplastik di Situ Kenanga dan Situ Mahoni pada semua sampel.

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The presence of microplastics in the water could threaten the biota there. This study was conducted to analyze the abundance, shapes, and colors of microplastics in water, sediment, gills and digestive tract of amazon sailfin catfish Pterygoplichthys pardalis from Situ Kenanga and Situ Mahoni, Universitas Indonesia Campus, Depok. Sampling of water and sediment were carried out at the inlet, midlet, and outlet of both situ. Water samples were filtered using plankton net, sediment samples were taken using Ekman grab and dried using an oven, as many as 15 individual P. pardalis samples were taken using cast net from each situ, then their gills and digestive tract were isolated and pulverized using 65% HNO3. Each sample obtained was dissolved with saturated NaCl solution for flotation to occur. Observations were made using a light microscope and Sedgwick Rafter Chamber to place each sample, by observing the shape, color, and number of microplastic particles. The results showed that the shapes of fibers, films, fragments, granules, as well as transparent, black, blue, and pink colors were found in all samples. The abundance of microplastics in Situ Kenanga water was 48.26 ± 23.51 particles L-1, sediment 45837.04 ± 36305.97 particles Kg-1, gills 290.48 ± 154.58 particles g-1 or 1156, 44 ± 378.69 ind-1 particles, digestive tract 134.37 ± 55.72 particles g-1 or 1364.89 ± 339.54 ind-1 particles. The abundance of microplastics in Situ Mahoni water was 48.63 ± 30.21 particles L-1, sediment 36237.04 ± 16702.60 particles Kg-1, gills 287.23 ± 109.40 particles g-1 or 1153, 78 ± 324.32 ind-1 particles, digestive tract 123.77 ± 34.35 particles g-1 or 1304.44 ± 270.90 ind-1 particles. There was no significant difference between the abundance of microplastics in Situ Kenanga and Situ Mahoni in all samples."

"Analisis Kelimpahan Mikroplastik pada Insang dan Saluran Pencernaan Ikan Sapu-sapu Pterygoplichtys pardalis (Castelnau, 1855), Air, dan Sedimen di Ciliwung Daerah Tanjung Barat dan Daerah MT Haryono, Jakarta Selatan

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Abundance Analysis of Microplastic in Gills dan Digestive Tract of Sailfin Catfish Pterygoplichthys pardalis (Castelnau, 1855), Water, and Sediment in Tanjung Barat Area and MT Haryono Area, Ciliwung, South Jakarta"

"Penelitian ini menganalisis bentuk dan kelimpahan mikroplastik pada sumber air laut, sumber air tawar petak A, air kolam 19, pakan buatan (pelet), saluran pencernaan, dan feses udang vannamei Litopenaeus vannamei (Boone, 1931) di Tambak Udang Vannamei Unit Sobo, Banyuwangi, Jawa Timur. Sampel air diambil dan disimpan dalam botol kaca sebanyak 300 ml, sampel pakan menggunakan pakan jenis SGH2 buatan perusahaan sendiri sebanyak 10 gr, sedangkan sampel udang digunakan sebanyak 5 individu dari kolam 19 dan tiap udang diambil bagian saluran pencernaan dan fesesnya. Sampel air, pakan, saluran pencernaan, dan feses diamati menggunakan mikroskop serta dilakukan pengulangan sebanyak 3 kali. Sampel diambil pada tanggal 25 Oktober 2021 dengan DOC (Day of Culture) 53 dan penelitian berlangsung selama 1 bulan dari bulan Oktober hingga November 2021. Penelitian memperlihatkan tiga bentuk mikroplastik, yaitu fragmen, film, dan fiber. Mikroplastik bentuk fragmen mendominasi dalam penelitian ini. Selain itu, berdasarkan data kelimpahan mikroplastik, diketahui bahwa mikroplastik pada pakan banyak yang tidak ditemukan ke dalam proses pencernaan. Kemudian, diketahui juga tidak terdapat perbedaan nilai kelimpahan, serta terdapat hubungan kelimpahan mikroplastik pada saluran pencernaan dan feses udang vannamei.

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This study analyzed the shape and abundance of microplastics in seawater sources, plot A freshwater source, pond water, artificial feed, digestive tract, and feces of Litopenaeus vannamei (Boone, 1931) at Unit Sobo Shrimp Ponds, Banyuwangi, East Java. As much as 300 ml of water were taken and stored in glass bottles; feed samples made by the company itself, were taken as much as 10 grams; while shrimp samples were used as many as 5 individuals from 19 ponds, taken from the digestive tract and feces. Each sample were observed using a microscope and repeated 3 times. Samples were taken on October 25, 2021 with DOC (Day of Culture) 53 and the study lasted for 1 month from October to November 2021. Research shows three forms of microplastics, namely fragments, films, and fibers. Microplastic fragments dominate in this study. In addition, based on data on the abundance of microplastics, it is known that many microplastics in feed are not found in the digestive process. Then, it was also known that there was no difference in the abundance value and that there was a relationship between the abundance of microplastics in the digestive tract and feces."

"Penelitian bertujuan mengetahui keragaman spesies khamir dari saluran pencernaan lebah madu Apis cerana di apiari Desa Ciburial, Bandung. Sebanyak 48 isolat khamir dari saluran pencernaan Pollen-collecting bee (PCB) (27 isolat) dan Nectarcollecting bee (NCB) (21 isolat) diidentifikasi berdasarkan data sequence daerah internal transcribed spacer (ITS) rDNA dan dikarakterisasi secara morfologi untuk melengkapi hasil identifikasi. Hasil identifikasi molekuler menunjukkan bahwa 48 isolat khamir tersebut terdiri atas delapan genus dan 16 spesies. Sebanyak 12 spesies khamir ditemukan pada PCB dan sembilan spesies khamir ditemukan pada NCB. Candida cf. apicola, C. etchellsii, Debaryomyces hansenii, Rhodotorula mucilaginosa dan Zygosaccharomyces rouxii ditemukan pada PCB maupun NCB. Spesies-spesies khamir yang diperoleh secara taksonomi heterogen, yaitu termasuk ke dalam class Hemiascomycetes dari phylum Ascomycota (13 spesies) dan class Urediniomycetes dari phylum Basidiomycota (3 spesies).

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The aim of this study was to study the diversity of yeast species isolated from the digestive tract of honey bee Apis cerana in apiary in Ciburial, Bandung. A total of 48 yeast isolates from the digestive tract of pollen-collecting bees (27 isolates) and Nectar-collecting bee (21 isolates) were identified based on sequence data of internal transcribed spacers regions of ribosomal DNA (ITS rDNA). In addition of their sequence data, yeasts were also characterized morphologically. The results showed that those yeasts comprised of eight genera and 16 species. Twelve yeast species were found from PCB and nine yeast species were found from NCB. Candida cf. apicola, C. cf. azyma, C. etchellsii, C. naeodendra, C. orthopsilosis, Cryptococcus heveanensis, Debaryomyces hansenii, Rhodotorula mucilaginosa and Zygosaccharomyces rouxii were found both in PCB and NCB. Our molecular analysis showed that A. cerana harbors taxonomically diverse yeasts. They consisted of species belong to the class Hemiascomycetes of the phylum Ascomycota (13 species) and class Urediniomycetes of the phylum Basidiomycota (3 species)."