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Ditemukan 4 dokumen yang sesuai dengan query

Adji Sasongko

Studi ekologi pinna muricata linnaeus (Moluska: Pelecypoda) serta hubungannya dengan lamun cymodocea rotundata (Ehrenb. and Hempr.) Aschers, di Pulau Semak Daun, Kepulauan Seribu

"Telah dilakukan penelitian ekologi Pinna muricata yang mencakup kepadatan, morfometri cangkang, sudut posisi kedudukannya lerhadap garis pantai, serta hubungannya dengan kepadalan dan biomassa lamun Cymodocea rotundata di Pulau Semak Daun, Kepulauan Seribu, Teluk Jakarta. Hasil penelitian menunjukkan Pinna muricata hanya ditemukan di antara lamun Cymodocea rotundata di 6 transek dari 16 transek yang digunakan, 4 transek (transek 7- 10) di bagian Selatan dan 2 transek (transek 12 dan lj) di bagian Utara. Kepadatan terbanyak terdapat di bagian Selatan pulau (0,6 individu per m3}, sedangkan di bagian Utara puiau kepadalannya hanya mencapai 0,03 individu per m2. Pinna muricata mempunyai panjang cangkang yang berkisar antara 12,5 - 17 cm dengan rata-rata 14,25 cm, sedangkan lebar cangkang berkisar antara 7 - 11,4 cm dengan rata-rata 8,43 cm. Sudut kedudukan Pinna muricata terbanyak (17%) antara 130" - 139° terhadap garis pantai. Kepadalan dan biomassa akar lamun Cymodocea roiundata tidak berkorelasi atau tidak ada hubungannya dengan jumlah Pinna muricata, sedangkan bkimassa daun menunjukkan korelasi negative (R= -0,36).

We examined fan mussel Pinna muricata (Mollusca, Bivalvia) such as the density, shell morphomeiry, angel of shell position against the shoreline, as well as his correlations with the density and the biomass of seagrass Cymodocea rotundata in the Semak Daun Island, Jakarta Bay. Results of Ihe research pointed out Pinna muricata were found only in seagrass meadows Cymodocea rotundata in 6 transects from 16 transects lhat was used, 4 transects (transect 7 - 10) on the South and 2 transects (transect 12 and 13) at the North of Island. The Pinna density was recorded 0.6 individuals per m2 at the southern of the island, whereas at the northern of the island only reached 0.03 individuals per m2. The length and wide of shells were measured between 12,5 - 17 cm (average 14,25 cm) and between 7 ? 11,4 cm (average 8,43 cm) respectively. The most angle of shell positions were measured between 120° - 139" against the shoreline. The density and the leave biomass seagrass Cymodocea rotundata did not correlate with the density of Pinna, whereas the root biomass have negative correlation (R= - 0,36)."

[place of publication not identified]: Sains Indonesia, 2003

SAIN-8-1-2003-1

Artikel Jurnal Universitas Indonesia Library

Vita Damayanti

Potensi aktivitas antimikroba streptomyces sp. BCy hasil isolasi dari lamun (cymodocea rotundata) = Antimicrobial potential of streptomyces sp. BCy isolated from seagrass (cymodocea rotundata)

"Actinomycetes BCy berhasil diisolasi dari lamun Cymodocea rotundata, Pantai Prapat Agung Bali Barat, Indonesia. Identifikasi dengan 16S rRNA menunjukkan isolat termasuk marga Streptomyces. Penelitian bertujuan untuk mengkaji potensi aktivitas antimikroba Streptomyces sp. BCy yang ditumbuhkan pada medium Bushnell-Haas BH dengan penambahan glukosa 0,1 dan yeast extract 0,05 . Isolat diinokulasikan ke dalam 400 mL medium BH Broth lalu diinkubasi pada suhu 30oC selama 1 dan 2 minggu secara statis. Percobaan dilakukan sebanyak dua batch. Medium difiltrasi dan biomassa diukur.

Filtrat diekstraksi dengan etil asetat 1:1, v/v lalu ekstrak kasar ditimbang. Ekstrak disuspensikan dengan DMSO dan akuades 1:6, v/v untuk uji antimikroba dengan metode Kirby Bauer pada konsentrasi 5 dan 15 mg/mL. Mikroba uji yang digunakan adalah Escherichia coli NBRC 3301, Staphylococcus aureus NBRC 100910, dan Candida albicans UICC Y-29. Biomassa kering meningkat dari minggu kesatu 469,9 mg ke minggu kedua 667,2 mg . Namun, berat ekstrak kasar menurun dari minggu kesatu 24,7 mg ke minggu kedua 17,05 mg . Aktivitas antimikroba dari ekstrak kasar hanya mampu menghambat Staphylococcus aureus NBRC 100910 serta ukuran zona bening meningkat pada konsentrasi 15 mg/mL.

Actinomycetes BCy has been isolated from seagrass Cymodocea rotundata, Prapat Agung Coastal Bali Barat, Indonesia. Identification by 16S rRNA showed that isolate belongs to genus Streptomyces. The objective of this study is to analyze antimicrobial potential of Streptomyces sp. BCy which was grown in Bushnell Haas BH medium added with 0.1 glucose and 0.05 yeast extract. The isolate was inoculated into 400 mL BH Broth then incubated at 30oC for 1 and 2 weeks using static fermentation. The experiment was carried out in two batches. Medium was filtered and dry weight of biomass was measured.
Filtrate was extracted using ethyl acetate 1 1, v v and also measured for dry weight. The dried crude extract was resuspended in DMSO and aquades 1 6, v v and used for antimicrobial testing using Kirby Bauer method in 5 and 15 mg mL. The target microbes are Escherichia coli NBRC 3301, Staphylococcus aureus NBRC 100910, and Candida albicans UICC Y 29. Biomass increased from first 469.9 mg to second week 667.2 mg. However, crude extract decreased from first 24.7 mg to second week 17.05 mg. The antimicrobial activity of crude extract was able to inhibit Staphylococcus aureus NBRC 100910 and also had larger clear zone in 15 mg mL. "

Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2017

S69254

UI - Skripsi Membership Universitas Indonesia Library

Harxylen kinanti Purnomo

Uji bioaktivitas ekstrak lamun cymodocea rotundata asch. & schweinf. dan thalassia hemprichii (ehrenb. ex solms) asch. sebagai larvasida nyamuk aedes aegypti l. = Bioactivity test of cymodocea rotundata asch. & schweinf. and thalassia hemprichii (ehrenb. ex solms) asch. seagrass extract larvicide of aedes aegypti l. mosquito

"ABSTRACT

Lamun memiliki banyak senyawa aktif dan potensial di bidang kesehatan dan pengobatan. Cymodocea rotundata dan Thalassia hemprichii dikoleksi dari Pulau Pramuka TNKpS dan Karang Sewu TNBB. Sampel kemudian dipisahkan berdasarkan daun, rimpang dan akar. Setiap bagian dibuat menjadi simplisia dan diesktraksi menggunakan metanol (1:3; b/v). Semua ekstrak dikarakterisasi menggunakan HPLC dan diuji aktivitas larvasida terhadap larva instar III Aedes aegypti. Sebanyak 25 larva instar III Aedes aegypti dipindahkan ke 250 mL botol sampel yang berisi 100 mL ekstrak 1%. Terdapat dua kontrol yaitu akuades dan abate 1%. Mortalitas larva dicatat pada 12, 24 dan 48 jam. Ekstrak daun T. hemprichii memiliki persentase mortalitas tertinggi yaitu 100% pada 12 jam. Konsentrasi LC50 ekstrak daun T. hemprichii yaitu 0,56%. Hasil kromatogram organ spesies C. rotundata dan T. hemprichii menunjukkan tidak ada perbedaan. Dari hasil tersebut disimpulkan bahwa ekstrak daun T. hemprichii memiliki aktivitas yang paling potensial sebagai larvasida Ae. aegypti.

ABSTRACT

Seagrass contains bioactive compounds that are potential to be developed in health and medicinal application. Cymodocea rotundata and Thalassia hemprichii was collected from Pramuka Island TNKpS and Karang Sewu TNBB. Samples were cut into different parts i.e. leaf, rhizome, and root. Each part was dried as a powdered simplicia and extracted using methanol (1:3; w/v). All the extracts were characterized using HPLC and tested as larvicide against the larvae of Aedes aegypti. Batches of 25 early 3rd instar larvae of Ae. aegypti were transferred into 250 mL sample bottles containing 100 mL 1% extract. There are two control groups: abate (1%) and aquadest. The mortality of larvae was observed after 12, 24, and 48 hours. The leaves extract of T. hemprichii showed the highest mortality 100% after 12 h with LC50 concentration 0.56%. Chromatogram results from different species of C. rotundata and T. hemprichii showed a similar pattern of peaks. The results suggested that leaves extract of Thalassia hemprichii have the highest potential to be used as a larvicide against Ae. aegypti larvae."

2017

S70061

UI - Skripsi Membership Universitas Indonesia Library

Ginting, Christyan Natanael Harvey Davika

Studi Perbandingan Kelimpahan Mikroplastik pada Cymodocea rotundata, Sedimen, dan Air di Wilayah Pesisir Suaka Margasatwa Pulau Rambut, Teluk Jakarta Tahun 2022 dan 2023 = A Comparative Study of Microplastic Abundance in Cymodocea rotundata, sediment, and water at the Coastal of Rambut Island Wildlife Reserve, Jakarta Bay in 2022 and 2023

"Keberadaan mikroplastik telah mencemari dan mengganggu perairan di wilayah Indonesia. Penelitian ini dilakukan untuk mengetahui perbandingan kelimpahan mikroplastik pada lamun Cymodocea rotundata, sedimen, dan air Pulau Rambut, Teluk Jakarta tahun 2022 dan 2023. Pengambilan sampel dilakukan di tiga stasiun Pulau Rambut (Timur, Selatan, dan Barat). Sampel diambil sebanyak delapan individu lamun per stasiun, sedimen, dan air dengan metode random sampling. Sampel lamun dipotong sepanjang 2 cm lalu dikerik, sedimen dikeringkan lalu diberikan larutan jenuh NaCl, dan sempel air diberikan NaCl kemudian seluruh sampel diamati dibawah mikroskop. Rata-rata kelimpahan mikroplastik lamun C. rotundata pada tahun 2022 sebesar 42 partikel/cm dan pada tahun 2023 sebesar 44,46 partikel/cm. Rata-rata kelimpahan mikroplastik pada sedimen tahun 2022 sebesar 73,53 partikel/g dan pada tahun 2023 sebesar 79,56 partikel/g. Kelimpahan mikroplastik pada sampel air tahun 2022 sebesar 51,33 partikel/L dan pada tahun 2023 sebesar 53,78 partikel/L. Uji Korelasi Spearman menjelaskan bahwa kelimpahan mikroplastik sampel lamun dengan sedimen, air dengan sedimen, dan lamun dengan air memiliki korelasi positif yang kuat. Hasil Uji-T menyatakan terdapat perbedaan tidak signifikan kelimpahan mikroplastik pada lamun C. rotundata (sig. (2-tailed) 0,182>0,05) dan terdapat perbedaan signifikan kelimpahan mikroplastik sedimen tahun 2022 dan 2023 (sig. (2-tailed) 0,007<0,05). Hasil uji ATR-FTIR didapatkan kandungan polimer CA, ABS, HDPE, PMMA, PVC, dan PET pada sampel daun lamun C. rotundata.

The existence of microplastics has polluted and disturbed the waters in Indonesian territory. This research compared the abundance of microplastics i Cymodocea rotundata seagrass, sediment, and water on Rambut Island, Seribu Islands, Jakarta, in 2022 and 2023. Samples were taken as many as eight individuals of seagrass per station, sediment, and water by random sampling method. The seagrass samples were cut 2 cm long and scraped, the sediment was dried and then given a saturated solution of NaCl, and the water sample was given NaCl. Then all samples were observed under a microscope. The average microplastic abundance of C. rotundata seagrass leaves in 2022 was 42 particles/cm and in 2023, it was 4.46 particles/cm. The average abundance of microplastics in sediments in 2022 was 73.53 particles/g and in 2023, it was 79.56 particles/g. The abundance of microplastics in water samples in 2022 was 51.33 particles/L and in 2023, it was 53.78 particles/L. The Spearman Correlation Test explains that the microplastic abundance of seagrass with sediment, water with sediment, and seagrass with water samples had a strong correlation. The results of the T-test stated that there was no significant difference in the abundance of microplastics in seagrass leaves of C. rotundata (sig. (2-tailed) 0.182>0.05) and there was a significant difference in the abundance of microplastics in sediments in 2022 and 2023 (sig. (2-tailed) 0.007<0.05). The results of the ATR-FTIR test found the polymer content of CA, ABS, HDPE, PMMA, PVC, and PET in C. rotundata seagrass samples."

Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia;Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia;Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia;Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia;Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2023

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