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"Latar belakang penelitian: Identifikasi spesies Candida penting untuk diagnosis, penentuan jenis obat dan prediksi kepekaan jamur terhadap obat anti fungal. Selama ini identifikasi dilakukan dengan uji konvensional: fisiologi-morfologi, yang relatif lama, hingga diagnosis dini sukar ditegakkan. Mengatasi masalah tersebut telah dikembangkan medium kromogenik yang mampu membedakan beberapa spesies Candida berdasarkan warna koloni. medium kromogenik yang saat ini tersedia di Indonesia adalah CHROMagar-Candida.Tujuan penelitian: Membandingkan cara identifikasi Candida spp. dengan metode konvesional dan medium kromogenik CHROMagar Candida, serta mengetahui spesifisitas dan sensitivitasnyaMetodologi penelitian: Penelitian merupakan uji diagnostik. Sebanyak 134 sampel ditanam pada agar Sabouraud Dekstrosa dan dipurifikasi (340 isolat). Setiap isolat diidentifikasi dengan CHROMagar Candida, uji fisiologi dan morfologi (agar tajin/tepung jagung-Tween 80, dan uji pembentukan germ tube).Hasil dan kesimpulan: Dengan CHROMagar-Candida, dapat diidentifikasi 148 (43,5%) isolat, 192 (56,7%) tidak dapat diidentifikasi. Spesies yang teridentifikasi: C. tropicalis (21,5%) koloni berwama ungu di tengah pucat di tepi, C. albicans (11,8%) warna koloni hijau terang, C parapsilosis (5,9%) koloni berwarna putih hingga merah jambu pucat, C glabrata (2,1%) koloni merah jambu pucat dengan permukaan koloni halus, C krusei (0,3%) koloni merah jambu pucat dengan permukaan koloni kasar dan Trichosporon sp (2,1%) koloni berwarna abu-abu dengan tipe koloni halus dan kasar. Yang tidak dapatdiidentifikasi, C pelliculosa, C. guilliermondii, C. langeroni, C Intermedia, C mogii, C lusitaniae, C utilis, C fennica, C obtuse, C sphaerica, C famata dan R. rubra.Spesifisitas dan sensitivitas CHROMagar-Candida untuk identifikasi C trop/calls 80,8% dan 27,8%, C albicans 99,3% dan 65,5%, C parapsilosis 96,9% dan 100%, Trichosporon sp 100% dan 21,8%. CHROMagar-Candida tidak dapat menggantikan uji konvensional dalam mengidentifikasi Candida spp, terutama Candida non-C albicans.

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Identification Of Candida Species From Clinical Specimens, Using Chromogenic Medium, Physiology And Morphology Test.Background : Species identification of Candida is important to establish to diagnosis, to determine the medicine needed and also to predict susceptibility of fungi to antifungal drugs. Up to now, identification is conducting using conventional method i.e. physiology-morphology which is time consuming. Thus early diagnosis could not be established. To offer come this problem chromogenic medium has been develop to distinguish species of Candida based on the colour of colony. Chromogenic medium that find on Indonesia is CHROMagar-Candida.

Aim :To compare CHROMagar Candida and conventional method in identification of Candida spp. specificity and sensitivity of CHROMagar Candida was also determined. Research Methodology: This study diagnostic investigation using cross sectional design. Those were 134 samples plated on Sabouraud Dektrosa Agar/SDA than purified that yields 340 isolates. It is isolate was identified by CHROMagar Candida and conventional method.

Result and Conclusions: Using CHROMagar 148 (43.5%) isolates can be identified were as 192 (56.7%) could not be identified. Species that can be identify were : C. tropicalis (21.5%) with purple colour in the centre and pale purple at the edge of colony, C alb/cans (11.8%) with bright green colour, C parapsilosis (5.9%) with white to pale pink, C. glabrata (2.1%) has a pale pink colour and smooth surface, C krusei (0.3%) is pale pink and rough surface, and Trichosporon sp. (2.1%) is gray with smooth or rough surface. Species that can not be identified by CHROMagar-Candida were : C pelliculosa, C guilliermondii, C langeroni, C intermedia, C mogii, C.lusitaniae, C utilis, C fenica, C. obtuse, C. sphaerica, C fanata, and R. rubs

Specificity and sensitivity CHROMagar Candida identifying C. tropicalis is 80.8% and 27.8%, C. alb/cans is ' 99.3% and 65.5%, C.parapsllosis is 96.9% and 100%, Trichosporon sp is 100% and 21.8% consecutively. Although conventional can not replace by CHROMagar Candida especially for Candida non C alb/cans identifications."

"Standar Kementerian Kesehatan menyatakan bahwa setiap makanan dan minuman tidak boleh mengandung Escherichia coli. Tujuan penelitian adalah menentukan kualitas mikrobiologis dari makanan siap saji dan minuman yang dijajakan di kantin kampus FMIPA UI Depok. Sebanyak 15 sampel, terdiri dari 10 jenis makanan siap saji, empat jus yang berbeda, dan air keran diperkaya dalam medium Buffered Peptone Water BPW sebelum diuji koliform. Uji koliform dari setiap sampel dilakukan pada medium kromogenik Chromocult Coliform Agar - Enhanced Selectivity CCA - ES dan Harlequin E. coli Coliform Agar HEC dan medium fluorogenik Readycult Coliform 100 RC 100 pada suhu 37oC. Hasil menunjukkan bahwa semua sampel mengandung bakteri koliform non - E. coli dan 12 di antaranya mengandung Escherichia coli. Isolasi dari sampel memperoleh 12 strain E. coli dan 15 isolat koliform non-E. coli. Uji koliform fekal dilakukan dengan menggunakan medium RC 100 pada suhu 44,5oC serta diperkuat dengan uji indol menggunakan reagen Kovac rsquo;s. Hasil uji menunjukkan bahwa E. coli yang terdapat pada 12 sampel berasal dari fekal. Hanya 7 dari 15 isolat koliform non - E. coli merupakan koliform fekal non - E. coli. Berdasarkan hasil penelitian yang diperoleh dapat disimpulkan bahwa kualitas mikrobiologis dari makanan dan minuman siap saji kantin FMIPA UI tidak memenuhi standar dari Kementerian Kesehatan.

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The standards from the Ministry of Health state that food and drinks should be free of Escherichia coli. The aim of this research was to assess the microbiological quality of ready to eat foods and drinks that were offered at a canteen in the Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok. Fifteen samples comprising of 10 different foods, four different juices, and tap water were enriched in buffered peptone water BPW before tested for the presence of coliforms using two chromogenic media Chromocult Coliform Agar Enhanced Selectivity CCA ES and Harlequin E. coli agar HEC and a fluorogenic medium Readycult Coliform 100 RC 100 at 37oC. Results showed that all samples contained non E. coli coliforms and 12 of them contained Escherichia coli. Twelve E. coli strains and 15 non E. coli coliform isolates were isolated. Fecal coliform tests were conducted for the E. coli strains and coliform isolates by performing a coliform test with Readycult coliform 100 at 44.5oC. The tests were strengthened with an indole test that uses a Kovac rsquo s reagent. The tests showed that the isolated E. coli from 12 samples were of fecal origin while only 7 out of 15 coliform isolates were fecal non E. coli coliforms. It was concluded that the microbiological quality of the canteen rsquo s ready to eat food and drinks did not fulfill the standards from the Ministry of Health. "