Hasil Pencarian  ::  Simpan CSV :: Kembali

"This study was carried out to analyze the serum vitamin A / carotene concentration among Indonesian full term neonates in relation to maternal nutritional status and nutrients intake. One hundred and eight couples' (mothers + neonates) were recruited for the study. The serum vitamin A/carotene concentrate ion was determined by High Pressure Liquid Chromotography (HPLC) and Ultraviolet Absorbance/Spectrophotometric method (IVACG, 1982) and the dietary nutrient intake of the mothers was analyzed using a 2 day/24 hours recall method. Seventy one (67%) of the neonates were suffering from low vitamin A level ( (20 g/dl), the rest 35 (33%) were at an adequate vitamin A level (>20 / g/dl) and 13 (12%) of the mothers were suffering from low vitamin A level ( <20 /4g/di), the rest 95 (88%) were at an adequate vitamin A level. That mean serum vitamin A/carotene of the neonates was lower than those of their mothers. The mean serum vitamin A of the mothers was 30.7 ,wg/dl - v.53 and mean serum carotene of the mothers was 124.23g/dl - 49.66. The study concluded that there was no significant correlation between serum vitamin A of the neonates and serum vitamin A of their mothers (p } 0.1). It was found that? serum vitamin .A concentration and serum carotene of the mothers were positively correlated; also maternal serum carotene was positively related to serum carotene of the neonates. The beta-carotene intake, fat intake, protein intake were positively related to serum vitamin A of the mothers. Further investigation using larger numbers of samples and more controlled method was suggested to -scrutinize the influences of gestational age, dietary nutrients intake on the serum of vitamin A/carotene of the mothers. The study also pointed out the importance of providing .the pregnant mothers with sound information about nutrition in pregnancy."

"Kanker kolorektal merupakan jenis kanker dengan jumlah penderita terbanyak ketiga di dunia. Metode deteksi kanker kolorektal yang banyak digunakan yakni kolonoskopi, namun metode tersebut bersifat invasif dan mahal. Salah satu metode deteksi yang dapat digunakan sebagai alternatif adalah melalui deteksi penanda molekuler adalah CK20 pada Circulating Tumor Cells (CTC) yang terdapat dalam darah perifer. Permasalahan muncul akibat rendahnya jumlah CTC dalam darah. Deteksi gen CK20 pada serum darah berpotensi menjadi metode deteksi kanker kolorektal karena serum darah mengandung cell-free nucleic acid (cfNA) yang dilepaskan oleh sel tumor. Tujuan dari penelitian ini adalah untuk mendeteksi gen CK20 pada CTC dan serum darah dari penderita kanker kolorektal. Isolat RNA diisolasi dari sampel CTC dan serum darah yang berasal dari empat penderita kanker kolorektal RSUPN Cipto Mangunkusumo kemudian diamplifikasi melalui Quantitave RT-PCR dan dianalisis melalui metode Melt Curve Analysis (MCA). Hasil menunjukkan bahwa gen CK20 memiliki Tm ~81°C dan terdeteksi pada satu sampel serum dan dua sampel jaringan. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa gen CK20 tidak terdeteksi pada CTC dan terdeteksi pada satu serum darah penderita kanker kolorektal.

---

Colorectal cancer occupies the third-highest number of cancer patients worldwide. The most widely used colorectal cancer detection method is colonoscopy. However, colonoscopy is invasive and expensive. One alternative detection method is the detection of molecular markers such as CK20 in circulating tumor cells (CTC) found in the peripheral blood. The problem arises due to the low number of CTCs in the blood. Detection of the CK20 gene in blood serum is potentially used as a colorectal cancer detection method because blood serum contains cell-free nucleic acid (cfNA) released by tumor cells. This study aimed to detect the CK20 gene in CTCs and blood serum from colorectal cancer patients. RNA isolates were isolated from CTC samples and blood serum from four colorectal cancer patients at Cipto Mangunkusumo Hospital and then amplified by quantitative RT-PCR and then analyzed using the Melt Curve Analysis method. The results showed that the CK20 gene had a melting temperature of an average of ~81°C and was detected in one blood serum sample and two cancer tissue samples. Based on the results obtained, it can be concluded that the CK20 gene was undetected in CTCs and detected in one blood serum of colorectal cancer patients."

"Kulit artifisial adalah susunan biomaterial yang terdiri dari sel, perancah, dan molekul bioaktif dan diaplikasaikan sebagai pengganti kulit yang rusak dalam terapi luka kronis. Perancah yang kompatibel secara biologis maupun fisikokimia masih menjadi fokus penelitian dari pengembangan kulit artifisial saat ini. Polikaprolakton (PCL) memiliki potensi sebagai material penyusun perancah karena biokompatibilitas, sifat mekanik, dan fleksibilitasnya. Namun, PCL memiliki bioaktivitas yang rendah sehingga perlu ditambahkan suatu bahan alami. Pada penelitian ini, Umbilical Cord Blood Serum atau Umbilical Cord Blood Serum (UCBS) dan Platelet-Rich Plasma (PRP) yang kaya akan molekul bioaktif dan matriks ekstraseluler ditambahkan ke perancah PCL sebagai coating. Perancah PCL difabrikasi terlebih dahulu dengan PCL 20% dengan metode freeze-drying. Kemudian, perancah di-coating dengan UCBS atau PRP dengan metode dip-coating dan gelasi termal atau dengan CaCl2. Pada penelitian ini, kedua coating meningkatkan biokompatibilitas perancah pcl yaitu perlekatan sel (78.90±3.65%-89.45±3.65%) dan viabilitas sel hingga (84.99%-99.23±3.72%). Perancah yang di-coating memiliki kekuatan tekan 2.78±0.005-3.58±0.64 Mpa) berpermukaan kasar, tingkat swelling 33.48±3.32%-50.15±1.39%), dan porositas 1.24±0.27%-1.79±0.12%. Maka dari itu, baik UCBS maupun PRP dapat meningkatkan biokompatibilitas perancah PCL sebagai kulit artifisial.

---

Artificial skin is a construct of biomaterials consisting of cells, scaffolds and bioactive molecules, and it is used as a substitute for damaged skin in chronic wound therapy. Scaffolds that are compatible both biological and physicochemical aspects are still the focus of research from the development of artificial skin recently. Polycaprolactone (PCL) has potential as a material for scaffold due to its biocompatibility, mechanical properties, and flexibility. However, PCL has low bioactivity, so it is necessary to add a natural ingredient. In this study, Umbilical Cord Blood Serum (UCBS) and Platelet Rich-Plasma (PRP) which are rich in bioactive molecules and extracellular matrix incorporated to the PCL scaffolds as coatings. The PCL scaffolds were fabricated with 20% PCL by freeze-drying method. Then, the scaffolds were coated with UCBS or PRP by dip-coating and gelation by temperature for UCBS and CaCl2 for PRP to polymerize extracellular matrix in UCBS and fibrin matrix in PRP. In this study, both coatings increased the biocompatibility of the PCL scaffold, including cell attachment (78.90±3.65%-89.45±3.65%) and cell viability (84.99%-99.23±3.72%). The coated scaffolds also improved physicochemical property including compressive strength (2.78±0.005-3.58±0.64 Mpa), rough surface, swelling ratio (33.48±3.32%-50.15±1.39%), and a porosity of 1.24±0.27%-1.79±0.12%. Therefore, both UCBS and PRP can be used as coatings to increase the biocompatibility of PCL scaffolds as artificial skins."

"Kulit buah delima (Punica granatum Linn) mengandung senyawa asam elagat yang potensial sebagai SERMs alami. Penelitian bertujuan untuk mengetahui apakah pemberian fraksi air kulit buah delima (FA) bermanfaat sebagai terapi hormonal pada defisiensi estrogen, dibandingkan dengan obat standar 17- estradiol dan tamoxifen. Metode ovariektomi digunakan untuk mewakili kondisi hipoestrogen menggunakan 42 tikus betina galur Sprague Dawley usia 50 hari yang diinduksi osteoporosis selama 21 hari (kecuali sham), dibagi menjadi 7 kelompok: sham; OVX; OVX-estradiol (0,1 mg/kg BB, p.o.); OVX-tamoxifen (10 mg/kg BB p.o.); OVX-FA dengan variasi dosis 50; 100; dan 200 mg/kg BB, p.o). Perlakuan diberikan setiap hari selama 28 hari.Hasil penelitian menunjukkan bahwa dibandingkan kelompok OVX, kelompok FA dosis 200 mg/kg BB mengalami peningkatan rerata kadar hormon estradiol darah (pg/ml) dari 46,11 + 3,301 menjadi 91,78 + 18,382 (p<0,05), peningkatan rerata kadar kalsium darah (mg/dl) dari 379,38 + 55,684 menjadi 475,43 + 38,321 (p<0,05), peningkatan rerata kadar fosfor darah (mg/dl) dari 30,74 + 21,697 menjadi 74,96 + 8,413 (p<0,05) dan rerata penambahan bobot badan tikus (gram) berkurang, dari 77,28 + 22,979 menjadi 32,55 + 18,408 (p<0,05). Sedangkan rerata kadar kolesterol total darah (mg/dl) kelompok FA dosis 50 mg/kg BB mengalami penurunan (p<0,05) (79,66 + 10,936) dibandingkan kelompok OVX (102,82 + 5,761). Disimpulkan bahwa pemberian FA mampu meningkatkan kadar estradiol darah yang berefek pada penurunan kadar kolesterol total darah dan mengurangi penambahan bobot badan pada tikus ovariektomi serta berefek pada parameter yang terkait bone remodelling yaitu mampu meningkatkan kadar kalsium darah dan mempertahankan homeostatis kadar fosfor dalam darah.

---

Pomegranate pericarp contain ellagic acid which potential as natural SERMs. This study aim to determine whether administration of pomegranate pericarp water fraction (FA) useful as hormonal therapy for estrogen deficiency, compare to 17-estradiol and tamoxifen, with forty-two-50-days-old female, 21 days-induced osteoporosis (except sham), assigned into 7 groups: SHAM; OVX; OVX-estradiol (0,1 mg/kgBW); OVX-tamoxifen (10 mg/kgBW); OVX-FA (50; 100 ; and 200 mg/kgBW). Treatment was given for 28 days.The results showed, compared to OVX, administration of FA 200 mg/kgBW increased blood estradiol hormones levels (pg/ml) from 46,11 + 3,301 to 91,78 + 18,382 (p<0,05), increased blood calcium levels (mg/dl) from 379,38 + 55,684 to 475,43 + 38,321 (p <0,05), increased blood phosphorus levels (mg/dl) from 30,74 + 21,697 to 74,96 + 8,413 (p <0,05) and reduced body weight gain (gr) from 77,28 + 22,979 to 32,55 + 18,408 (p<0,05). While at FA 50 mg/kgBW, blood total cholesterol levels (mg/dl) is decreased (p<0,05) (79,66 + 10,936) compare to OVX (102,82 + 5,761). Administration of FA increase blood estradiol levels, decrease blood total cholesterol levels, reduce body weight gain of ovariectomized rats, and on parameters related to bone remodeling it can increase blood calcium levels and maintain homeostatic of blood phosphorus levels."