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Abstrak :
Pendahuluan: Sindrom Ovarium Polikistik SOPK merupakan gangguan endokrin tersering penyebab infertifilitas pada wanita usia reproduktif. Wanita-wanita dengan SOPK diketahui memiliki tingkat apoptosis yang rendah dibandingkan dengan wanita tanpa SOPK dan memiliki kadar Anti-Muellerian Hormone AMH yang lebih tinggi dibandingkan dengan wanita tanpa SOPK. Akan tetapi, belum ada penelitian yang menghubungkan kadar AMH dengan apoptosis yaitu adanya atresia folikel pada ovarium wanita penderita SOPKTujuan: Untuk mengetahui hubungan antara kadar AMH yang tinggi dengan tingkat apoptosis sel granulosa yang terjadi pada pasien SOPK sehingga dapat diketahui salah satu patogenesis kelainan folikulogenesis pada pasien SOPKMetodologi: Studi cross sectional dengan mengambil sampel sel granulosa wanita SOPK dan tanpa SOPK atau kelainan ovarium yang mengikuti program Fertiisasi In Vitro FIV di Yasmin dan SMART-IVF, klinik dr. Sander B Jakarta. Jumlah sampel yaitu 40 sampel yang terdiri dari 20 wanita dengan SOPK dan 20 wanita tanpa SOPK. Tingkat apoptosis dievaluasi dengan mengukur ekspresi mRNA dari gen pengkode protein keluarga apoptotic Bcl2 Bax dan Bcl2 menggunakan metode kuantitatif absolut qPCR. Pengukuran kadar AMH di serum dilakukan dengan metode ELISA.Hasil: Terdapat perbedaan bermakna secara statistik antara kadar AMH wanita SOPK dan kontrol pABSTRACT
Background Polycystic Ovary Syndrome PCOS is a common endocrine abnormality in causing infertility in reproductive aged women. Women with PCOS were reported have lower apoptosis rate compared to women without PCOS and have higher level Anti Muellerian Hormone compared to women without PCOS. However, there are no reported studies which directly study to know correlation between AMH level in serum and apoptosis result in follicle atresia in ovarium of PCOS patients.Objective To analysis correlation between serum AMH level and apoptosis in granulosa cell in PCOS pasien that may underlie the folliculogenesis abnormality in PCOS.Methods Cross sectional study of sample from granulose cells women with PCOS and without PCOS or with ovarian abnormalities that following Fertility In Vitro FIV program in Yasmine and SMART ndash IVF, dr. Sander B clinic, Jakarta. Sample number were 40 consisting 20 women with PCOS and 20 women without PCOS. Apoptosis level were evaluated with measuring mRNA expression from gene that of coding apoptotic Bcl2 family Bax and Bcl2 using quantitave absolute method qPCR. AMH level in serum were measured using ELISA method.Results There was a statistical significance difference AMH level between PCOS group and control group p

Abstrak :
Apoptosis or programmed cell death is a normal condition for development and live multicellular organism. Apoptosis is a morphological phenomenon that play important role in physiological processes during fetal development and in adult. Mitochondria play an important role in apoptosis. Mitochondria can do apoptosis directly. Mitochondria has 2 family of protein Bcl-2. Bcl-2 and Bcl-XL are anti apoptosis while Bad and Bax are pro apoptosis. There are 3 different mechanism apoptosis. One generated by signals arising within the cell another triggered by death activators binding to receptors at the cell surface and a third may be triggered by dangerous agent that different from two ways before. Apoptosis also need caspase as cell death executor. Study of apoptosis still done especially in case of disease. Some disease have known related with disturbing of apoptosis mechanism for example cancer and auto immun. This article reviews about molecular mechanism of apoptosis for understanding disease and future therapy.

Abstrak :
Tesis ini bertujuan untuk memperoleh upaya preservasi fungsi ovarium yang efektif dengan penilaian apoptosis sel granulosa. Vitrifikasi korteks ovarium menjadi pilihan dalam upaya mempertahankan fungsi reproduksi wanita penderita kanker karena dengan teknik ini dapat disimpan banyak folikel primordial, dilakukan kapan saja saat siklus haid tanpa penundaan terapi kanker dan dapat dilakukan untuk pasien prepubertas dan belum menikah. Penelitian vitrifikasi korteks ovarium masih terbatas pada hewan coba serta belum terdapat data yang menilai kejadian apoptosis sel granulosa pasca vitrifikasi korteks ovarium manusia yang dilihat dari ekspresi gen terkait apoptosis. Penelitian ini merupakan penelitian kuasi eksperimental yang dilaksanakan di Departemen Obstetri Ginekologi Fakultas Kedokteran Universitas Indonesia - RSUPN Dr. Cipto Mangunkusumo dan RS Fatmawati Jakarta dalam rentang waktu Maret 2012 hingga Mei 2015. Korteks ovarium didapatkan dari tiga belas pasien berusia 31-37 tahun yang menjalani ooforektomi atas indikasi ginekologis. Secara morfologi, folikel dari korteks ovarium segar tidak terdapat perbedaan dibandingkan dengan dari korteks ovarium pasca vitrifikasi. Rerata ekspresi protein Bax dari korteks ovarium segar yang dinilai dalam bentuk H-score adalah 1,66 ± 0,14 dibandingkan 1,68 ± 0,13 pada ovarium pasca vitrifikasi (p = 0,165). Sedangkan rerata ekspresi protein Bcl-2 dari korteks ovarium segar adalah 1,73 ± 0,10 dibandingkan 1,71 ± 0,10 pada ovarium pasca vitrifikasi (p = 0,068). Vitrifikasi korteks ovarium terbukti tidak menyebabkan peningkatan ekspresi gen Bax dan Bcl-2. ......The aim of this study was to obtain the effective method of ovarian function preservation with granulose cell apoptosis assessment. Ovarian tissue vitrification became a method for ovarian function preservation in women with cancer. This technique can be done anytime without delay on cancer therapy, in prepubertal and unmarried patient. It also can store many primordial follicles. Ovarian tissue vitrification study is still limited to animal test and there was no data about apoptosis assessment after ovarian vitrification in human ovary. This is a quasi experimental study which was held in Department of Obstetrics and Gynecology Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital and Fatmawati Hospital Jakarta from March 2012 to May 2015. Ovaries from thirteen women between 31-37 years of age who underwent oophorectomy with gynecological indication were examined. There were no difference morphologically between follicles from fresh and warmed-vitrified ovaries. The mean protein Bax expression on the fresh ovaries which assessed in the form of H-score was 1,66 ± 0,14 compared to 1,68 ± 0,13 on the warmed-vitrified grup (p = 0,165). The mean protein Bcl-2 expression on the fresh ovaries which assessed in the form of H-score was 1,73 ± 0,10 compared to 1,71 ± 0,10 on the warmedvitrified grup (p = 0,068). As a conclusion, it was shown that vitrification did not affect Bax and Bcl-2 expression on human ovary.

Abstrak :
Pengantar: Endometriosis merupakan salah satu penyebab infertilitas dan menjadi indikasi fertilisasi in vitro (FIV). Laju apoptosis dan stress oksidatif yang tinggi pada pasien endometriosis diyakini menimbulkan efek negatif terhadap peluang keberhasilan FIV. Namun, pengaruh endometriosis terhadap keberhasilan FIV menunjukkan bukti yang inkonsisten dan belum banyak studi yang menilai langsung efek endometriosis terhadap kualitas oosit sebagai parameter keberhasilan FIV. Tujuan: Untuk menilai laju apoptosis pada sel granulosa pasien endometriosis dibanding pasien non-endometriosis melalui rasio ekspresi mRNA BAX/BCL-2 dan menilai korelasinya dengan kualitas oosit yang didapatkan saat petik ovum. Hasil: Sampel didapatkan dari 15 subjek dengan endometriosis dan 15 subjek kontrol. Dosis rekombinan FSH total yang diterima pada kelompok endometriosis untuk stimulasi ovarium lebih tinggi dibandingkan kelompok kontrol (p=0.005). Terdapat perbedaan bermakna kadar ekspresi BAX (p=0.029) dan BCL-2 (p<0.001) pada kedua kelompok, tetapi perbedaan rasio keduanya tidak signifikan (p=0.787). Korelasi antara rasio BAX/BCL-2 dengan parameter kualitas oosit tidak menunjukkan hubungan bermakna di kedua kelompok. Kesimpulan: Tidak terdapat perbedaan signifikan pada rasio kadar BAX/BCL-2 di kedua kelompok dan tidak ditemukan hubungan bermakna antara rasio tersebut dengan kualitas oosit.  ...... Introduction: Endometriosis is one of common conditions causing infertility and an indication to undergo in vitro fertilization (IVF). High apoptosis rate and oxidative stress in patient with endometriosis is believed to cause negative effect on IVF success rate. However, there has been conflicting results on endometriosis effect to IVF success and there have been limited studies that directly assess endometriosis and its effect on oocyte quality. Aim: To assess apoptosis rate on granulosa cells in patients with endometriosis compared to non-endometriosis patients through mRNA BAX/BCL-2 ratio and how it correlates with oocyte quality collected during ovum pick up. Results: Samples were collected from 15 subjects with endometriosis and 15 control subjects. Total dose of recombinant FSH received by endometriosis group is significantly higher compared to control (p=0.005). There is difference in BAX level (p=0.029) and BCL-2 level (p<0.001) in both groups. However, the ratio does not differ significantly (p=0.787). No significant correlation is found in BAX/BCL-2 ratio and any of the oocyte quality parameters. Conclusion: We found no significant difference in BAX/BCL-2 ratio between endometriosis and control group as well as significant correlation between the ratio and oocyte quality.

Abstrak :
ABSTRAK
B-cell leukaemia/lymphoma-2 Bcl-2 dan Bcl-2-associated X protein Bax merupakan anggota dari Bcl-2 family yang berperan dalam meregulasi apoptosis. Apoptosis penting dalam perkembangan manusia. Terganggunya kejadian apoptosisakan memberikan efek terhadap keadaan fisiologis manusia, diantaranya gangguan reproduksi. Salah satu gangguan reproduksi yang dialami oleh wanita usia reproduktif yaitu Sindrom Ovarium Polikistik (SOPK). Wanita penderita SOPK memiliki jumlah folikel yang lebih banyak dibandingkan wanita tanpa SOPK. Penelitian bertujuan untuk mengetahui ekspresi mRNA bax dan bcl-2 pada wanita SOPK serta mengetahui korelasi rasio ekspresi mRNA bax terhadap bcl-2 dengan rasio fertilisasi. Sel granulosa untuk penelitian didapatkan dari 18 wanita penderita SOPK dan 10 wanita tanpa SOPK yang sedang menjalani program FIV. Hasil penelitian menunjukkan tidak ada perbedaan yang berarti antara rasio ekspresi mRNA bax/bcl-2 wanita penderita SOPK dan tanpa SOPK p > 0,05. Korelasi yangkuat R = 0,525 ditemukan antara rasio ekspresi bax/bcl-2 dengan rasio fertilisasi.

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ABSTRACT
B cell leukemia lymphoma 2 Bcl 2 and Bcl 2 associated X protein Bax are members of the Bcl 2 family that play a role regulating apoptosis. Apoptosis plays an important role in human development. Disruption of apoptosis will have an effect on the physiological state of humans, including reproductive disorders. One of there productive disorders experienced by women in reproductive age is Polycystic Ovary Syndrome (PCOS). Women with PCOS have a higher number of follicles than women without PCOS. The aim of this study was to find out the expression of baxand bcl 2 mRNA expression in PCOS women and to know the correlation of bax mRNA expression ratio to bcl 2 with fertilization ratio. Granulosa cells for the study were obtained from 18 women with PCOS and 10 women without PCOS undergoing IVF program. The results showed no significant difference between the expression ratio of bax bcl 2 mRNA women with PCOS and without PCOS p 0.05. A strong correlation R 0.525 was found between the expression ratio of bax bcl 2and the fertilization rate.

Abstrak :
[ABSTRAK
Latar Belakang: Kanker prostat adalah kanker yang paling umum pada pria. Kanker terjadi karena hilangnya kontrol atas proliferasi sel dan apoptosis sehingga sel berproliferasi terus menerus tanpa ada kematian sel. Apoptosis diregulasi oleh beberapa protein tertentu diantaranya protein keluarga Bcl-2 dan protein kanal. Perkembangan kanker prostat memerlukan transformasi dari sel epitel yang normal menjadi sel ganas yang kehilangan kemampuan untuk mengakumulasi zinc. Salah satu efek utama zinc adalah mencegah pertumbuhan sel kanker prostat dengan menginduksi apoptosis dengan memfasilitasi proses pembentukan pori Bax yang memulai apoptogenesis mitokondria. Selain keluarga Bcl-2, VDAC1 juga berperan penting dalam proses apoptosis. Beberapa penelitian menyatakan Bcl-2 mempunyai kaitan erat dengan VDAC1 terkait proses apoptosis dan protein pro-apoptotik Bax juga secara langsung berinteraksi dengan VDAC yang kemudian menginduksi keluarnya sitokrom c dari membran mitokondria. Tujuan: Mengevaluasi ekspresi mRNA dari gen mengkode keluarga protein Bcl-2 (Bax dan Bcl-2) dalam proses apoptogenesis pada galur sel kanker prostat yg diinduksi oleh zinc; Mengevaluasi ekspresi mRNA dari gen VDAC1 dalam proses apoptogenesis pada galur sel kanker prostat yang diinduksi oleh zinc; Menganalisis hubungan antara ekspresi VDAC1 dengan protein keluarga Bcl-2 pada apoptogenesis galur sel kanker prostat. Desain: Penelitian ini menggunakan eksperimental in vitro dan analisis statistik Metode: Untuk memperbanyak galur sel kanker prostat (PC3) dilakukan kultur sel, kemudian diberi perlakuan dengan tiga kelompok (kontrol, zinc 20 μM dan staurosporin 0,16 μM). Selanjutnya dilakukan isolasi RNA dan elektroforesis RNA untuk mengetahui keutuhan RNA. Terakhir dilakukan qRT PCR yang kemudian datanya dianalisis secara statistika. Hasil: Ekspresi Bax, Bcl-2 dan VDAC1 pada galur sel kanker prostat (PC-3) yang diberi perlakuan zinc mengalami penurunan dibandingkan dengan kontrol (tidak diberi perlakuan). Akan tetapi penurunan ekspresi tersebut tidak bernilai signifikan karena nilai p > 0,05 (nilai signifikansi Bax = 0,309; nilai signifikansi Bcl-2 = 0,236; nilai signifikansi VDAC1 = 0,437). VDAC1 mempunyai korelasi yang signifikan (p < 0,05) dengan Bax (p = 0,01) dibandingkan dengan Bcl-2 (p = 0,118). Kesimpulan: Terjadi perubahan ekspresi pada setiap gen (Bax, Bcl-2 dan VDAC1) pada galur sel kanker prostat yang diberi perlakuan zinc dengan yang tidak diberi perlakuan, akan tetapi tidak bernilai signifikan. VDAC1 mempunyai korelasi yang bermakna dengan Bax dan mempunyai korelasi yang tidak bermakna dengan Bcl-2. ABSTRACT
Background: Prostate cancer is the most common cancer in men. Cancer occurs due to loss control of cell proliferation and apoptosis thus continuously proliferating cells without cell death. Apoptosis is regulated by specific proteins including Bcl-2 family proteins and channel proteins. The development of prostate cancer requires the transformation of normal epithelial cells into malignant cells that lose the ability to accumulate zinc. One of the main effects of zinc is to prevent the growth of prostate cancer cells by inducing apoptosis by facilitating the process of pore formation Bax that started apoptogenesis mitochondrial. In addition to Bcl-2 family, VDAC1 also plays an important role in the process of apoptosis. Some studies suggest Bcl-2 has close links with related VDAC1 apoptosis and pro-apoptotic protein Bax also directly interact with VDAC which then induces the release of cytochrome c from the mitochondrial membrane. Objective: To evaluate the expression of mRNA of the gene encoding the Bcl-2 family proteins (Bax and Bcl-2) in the process apoptogenesis on prostate cancer cell line that is induced by zinc; Evaluate the mRNA expression of genes in the process VDAC1 apoptogenesis on prostate cancer cell line induced by zinc; Analyzing the relationship between the expression of VDAC1 with Bcl-2 family proteins in prostate cancer cell lines apoptogenesis. Design: This study used an experimental in vitro and statistical analysis Methods: To reproduce the prostate cancer cell lines (PC3) performed cell culture, then treated with three groups (control, zinc 20 μM and staurosporin 0,16 μM). Furthermore, the isolation of RNA and RNA electrophoresis to determine the integrity of the RNA. Recently performed qRT PCR and the data were analyzed statistically. Results: The expression of Bax, Bcl-2 and VDAC1 on prostate cancer cell line (PC-3) were treated with zinc decreased than the control (untreated). However, a decrease in the expression of no significant value because the value of p > 0.05 (Bax significant value = 0.309; the value of the significance of Bcl-2 = 0.236; VDAC1 significant value = 0.437). VDAC1 has a significant correlation (p < 0.05) with Bax (p = 0.01) than Bcl-2 (p = 0.118). Conclusion: There is a change in the expression of each gene (Bax, Bcl-2 and VDAC1) in prostate cancer cell lines that treated with zinc than untreated, but no significant value. VDAC1 has a significant correlation with Bax and had no significant correlation with Bcl-2.;Background: Prostate cancer is the most common cancer in men. Cancer occurs due to loss control of cell proliferation and apoptosis thus continuously proliferating cells without cell death. Apoptosis is regulated by specific proteins including Bcl-2 family proteins and channel proteins. The development of prostate cancer requires the transformation of normal epithelial cells into malignant cells that lose the ability to accumulate zinc. One of the main effects of zinc is to prevent the growth of prostate cancer cells by inducing apoptosis by facilitating the process of pore formation Bax that started apoptogenesis mitochondrial. In addition to Bcl-2 family, VDAC1 also plays an important role in the process of apoptosis. Some studies suggest Bcl-2 has close links with related VDAC1 apoptosis and pro-apoptotic protein Bax also directly interact with VDAC which then induces the release of cytochrome c from the mitochondrial membrane. Objective: To evaluate the expression of mRNA of the gene encoding the Bcl-2 family proteins (Bax and Bcl-2) in the process apoptogenesis on prostate cancer cell line that is induced by zinc; Evaluate the mRNA expression of genes in the process VDAC1 apoptogenesis on prostate cancer cell line induced by zinc; Analyzing the relationship between the expression of VDAC1 with Bcl-2 family proteins in prostate cancer cell lines apoptogenesis. Design: This study used an experimental in vitro and statistical analysis Methods: To reproduce the prostate cancer cell lines (PC3) performed cell culture, then treated with three groups (control, zinc 20 μM and staurosporin 0,16 μM). Furthermore, the isolation of RNA and RNA electrophoresis to determine the integrity of the RNA. Recently performed qRT PCR and the data were analyzed statistically. Results: The expression of Bax, Bcl-2 and VDAC1 on prostate cancer cell line (PC-3) were treated with zinc decreased than the control (untreated). However, a decrease in the expression of no significant value because the value of p > 0.05 (Bax significant value = 0.309; the value of the significance of Bcl-2 = 0.236; VDAC1 significant value = 0.437). VDAC1 has a significant correlation (p < 0.05) with Bax (p = 0.01) than Bcl-2 (p = 0.118). Conclusion: There is a change in the expression of each gene (Bax, Bcl-2 and VDAC1) in prostate cancer cell lines that treated with zinc than untreated, but no significant value. VDAC1 has a significant correlation with Bax and had no significant correlation with Bcl-2., Background: Prostate cancer is the most common cancer in men. Cancer occurs due to loss control of cell proliferation and apoptosis thus continuously proliferating cells without cell death. Apoptosis is regulated by specific proteins including Bcl-2 family proteins and channel proteins. The development of prostate cancer requires the transformation of normal epithelial cells into malignant cells that lose the ability to accumulate zinc. One of the main effects of zinc is to prevent the growth of prostate cancer cells by inducing apoptosis by facilitating the process of pore formation Bax that started apoptogenesis mitochondrial. In addition to Bcl-2 family, VDAC1 also plays an important role in the process of apoptosis. Some studies suggest Bcl-2 has close links with related VDAC1 apoptosis and pro-apoptotic protein Bax also directly interact with VDAC which then induces the release of cytochrome c from the mitochondrial membrane. Objective: To evaluate the expression of mRNA of the gene encoding the Bcl-2 family proteins (Bax and Bcl-2) in the process apoptogenesis on prostate cancer cell line that is induced by zinc; Evaluate the mRNA expression of genes in the process VDAC1 apoptogenesis on prostate cancer cell line induced by zinc; Analyzing the relationship between the expression of VDAC1 with Bcl-2 family proteins in prostate cancer cell lines apoptogenesis. Design: This study used an experimental in vitro and statistical analysis Methods: To reproduce the prostate cancer cell lines (PC3) performed cell culture, then treated with three groups (control, zinc 20 μM and staurosporin 0,16 μM). Furthermore, the isolation of RNA and RNA electrophoresis to determine the integrity of the RNA. Recently performed qRT PCR and the data were analyzed statistically. Results: The expression of Bax, Bcl-2 and VDAC1 on prostate cancer cell line (PC-3) were treated with zinc decreased than the control (untreated). However, a decrease in the expression of no significant value because the value of p > 0.05 (Bax significant value = 0.309; the value of the significance of Bcl-2 = 0.236; VDAC1 significant value = 0.437). VDAC1 has a significant correlation (p < 0.05) with Bax (p = 0.01) than Bcl-2 (p = 0.118). Conclusion: There is a change in the expression of each gene (Bax, Bcl-2 and VDAC1) in prostate cancer cell lines that treated with zinc than untreated, but no significant value. VDAC1 has a significant correlation with Bax and had no significant correlation with Bcl-2.]

Abstrak :

Kurkumin merupakan pigmen kuning alami dari rimpang kunyit yang diduga memiliki aktivitas kemopreventif terhadap sel kanker melalui mekanisme jalur pensinyalan apoptosis. Penelitian ini bertujuan untuk menguji hubungan kurkumin terhadap kadar protein RASSF1A,  Bax, dan aktivitas kaspase-3 dalam menunjang  mekanisme apoptosis pada sel kanker payudara CSA03, MCF-7, dan MDA-MB-468.

Penelitian eksperimen in vitro dilakukan di laboratorium terpadu Fakultas Kedokteran Universitas Indonesia Jakarta,  laboratorium terpadu Fakultas Kedokteran Universitas YARSI Jakarta, RSUPN Dr. Cipto Mangunkusumo Jakarta, serta RS Islam Jakarta tahun 2016–2018. Pemberian kurkumin terhadap sel kanker didasarkan atas perbedaan dosis dan waktu pemberian. Uji sitotoksisitas  setelah pemberian kurkumin ditentukan secara MTS.   Kadar protein RASSF1A dan Bax diuji secara ELISA. Aktivitas kaspase-3 digunakan untuk mengetahui apoptosis diuji secara flowsitometri. Selanjutnya perubahan morfologi sel diamati melalui pewarnaan acridine orange/ethidium bromide.

Pemberian kurkumin terhadap sel-sel yang diuji menunjukkan konsentrasi IC₅₀ yaitu 40,85 µg/mL pada sel CSA03; 75,73 µg/mL pada sel MCF-7; dan 380,79 µg/mL pada sel MDA-MB-468. Pemberian kurkumin menunjang mekanisme apoptosis melalui jalur RASSF1A, Bax, dan aktivitas kaspase-3 pada sel kanker payudara.

 

Kata Kunci:  Apoptosis, Bax, CSA03, kaspase-3, kurkumin,  MCF-7, MDA-MB-468, pewarnaan ganda, RASSF1A

 

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Curcumin is a natural yellow pigment from turmeric rhizome which is thought to have a chemopreventive effect on cancer through the mechanism of apoptotic signaling pathways. This study aims to examine the correlation of curcumin with protein level of RASSF1A, Bax, and caspase-3 activities in conjunction with the mechanism of apoptosis in CSA03, MCF-7, and MDA-MB-468 breast cancer cells.

In vitro experimental research was carried out at the Integrated Laboratory of Faculty of Medicine, Universitas Indonesia Jakarta; RSUPN. Dr. Cipto Mangunkusumo Jakarta; and Jakarta Islamic Hospital during 2016–2018. Curcumin was administered to the cancer cells in different doses and time. Cytotoxicity test after administration of curcumin was determined by MTS. The protein level of RASSF1A and Bax were measured by ELISA. Caspase-3 activity was used to determine apoptosis by flow cytometry. Furthermore, changes in cell morphology were observed by acridine orange/ethidium bromide staining.

The administration of curcumin to the cells showed IC50 concentrations of 40.85 µg/mL in CSA03 cells; 75.73 μg/mL in MCF-7 cells; and 380.79 µg/mL in MDA-MB-468 cells. The administration of curcumin supports the mechanism of apoptosis through the RASSF1A, Bax, and caspase-3 activity in breast cancer cells.

Abstrak :
Telah dilakukan penelitian untuk mengidentifikasi efek krioprotektif madu lengkeng (Dimocarpus longan) terhadap integritas struktur folikel preantral dan profil ekspresi protein apoptosis jalur intrinsik. Sebanyak 24 ekor tikus (Rattus norvegicus L) dikelompokkan menjadi 8 kelompok, terdiri atas KKN, KKV (NaCl 0,9%), KKP1 (EG 7,5%), KKP2 (EG 15%), KP1 (EG 7,5% + ML 7,5%), KP2 (EG 7,5% + ML 15%), KP3 (EG 15% + ML 7,5%), dan KP4 (EG 15% + ML 15%). Pengamatan terhadap densitas,struktur folikel serta ekspresi protein Bax,Bcl2 dan Caspase3 dilakukan terhadap sayatan ovarium yang dibuat dengan metode parafin dengan pewarnaan Hematoksilin-Eosin (HE) dan imunohistokimia. Antibodi primer yang digunakan adalah antibodi poliklonal Rabbit Anti-Bax (A00183 Boster, USA), Rabbit Anti-Bcl-2 (A00040-2 Boster, USA) dan Active Caspase-3 Rabbit Polyclonal Antibody (ab4051 Abcam, UK) dan One Step Neopoly Detection System Kit (BGNK-0025 Biogear, USA). Identifikasi terhadap tiap tipe folikel preantral menggunakan mikroskop cahaya yang terhubung dengan perangkat lunak Image Raster dan IHC profiler. Hasil penelitian menunjukkan, efek krioprotektif madu lengkeng dapat meningkatkan densitas folikel, indeks folikel intak G2 dan G3, menurunkan indeks folikel G1, menekan ekspresi protein Bax dan caspase 3 serta meningkatkan ekspresi protein Bcl2. Dengan demikian, madu lengkeng memiliki potensi untuk dikembangkan sebagai krioprotektan ekstraselular alami dalam aplikasi vitrifikasi ovarium. ......A study was conducted to identify the cryoprotective effect of longan honey (Dimocarpus longan) on the structural integrity of the preantral follicle and the expression profile of the intrinsic pathway of apoptosis protein. A total of 24 rats (Rattus norvegicus L) were grouped into 8 groups, consisting of KKN, KKV (NaCl 0.9%), KKP1 (EG 7.5%), KKP2 (EG 15%), KP1 (EG 7.5% + LH 7.5%), KP2 (EG 7.5% + LH 15%), KP3 (EG 15% + LH 7.5%), and KP4 (EG 15% + LH 15%). Observations on the density, follicular structure and protein expression of Bax, Bcl2 and Caspase3 were carried out on ovarian sections made by paraffin method with Hematoxylin-Eosin (HE) staining and immunohistochemistry. The primary antibodies used were Rabbit Anti-Bax polyclonal antibody (A00183 Boster, USA), Rabbit Anti-Bcl-2 (A00040-2 Boster, USA) and Active Caspase-3 Rabbit Polyclonal Antibody (ab4051 Abcam, UK) and One Step Neopoly Detection System Kit (BGNK-0025 Biogear, USA). Identification of each type of preantral follicle using a light microscope connected to Image Raster software and an IHC profiler. The results showed that the cryoprotective effect of longan honey could increase follicle density, G2 and G3 intact follicle index, decrease G1 follicle index, suppress Bax protein expression and caspase 3 and increase Bcl2 protein expression. Thus, longan honey has the potential to be developed as a natural extracellular cryoprotectant in ovarian vitrification applications.

Abstrak :
ABSTRAK
Endometriosis merupakan penyakit pada sistem reproduksi wanita yang ditandai dengan tumbuhnya jaringan endometrium di luar rongga uterus. Endometriosis memengaruhi sistem reproduksi salah satunya dengan menyebabkan penurunan kualitas oosit akibat terjadinya apoptosis pada sel granulosa di dalam folikel. Apoptosis pada sel granulosa diketahui diaktivasi melalui jalur intrinsik yang dipengaruhi oleh protein BAX (pro-apoptosis) dan BCL-2 (anti-apoptosis). Penelitian ini bertujuan untuk mengetahui ekspresi mRNA bcl-2 dan bax pada sel granulosa penderita endometriosis melalui metode real-time PCR yang kemudian diuji secara statistik dengan menggunakan Uji-t. Hasil penelitian menunjukkan bahwa ekspresi mRNA bax/bcl-2 pada wanita penderita endometriosis menunjukkan perbedaan yang signifikan (p < 0,05) dibandingkan pada wanita tanpa endometriosis.

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ABSTRACT
Endometriosis is a disease in female reproductive system which marked by the present of endometrium tissue outside the uterus cavity. Endometriosis affects the reproductive system by decreasing oocyte quality as an impact of granulosa cells apoptosis in the follicle. Apoptosis in granulosa cells has been known activated through intrinsic pathway which is influenced by BAX (pro-apoptosis) and BCL-2 (anti-apoptosis) proteins. This research was conducted to know the mRNA expression of bcl-2 and bax in granulosa cells of endometriosis patients using real-time PCR and statistic tests (T-test). The result shows that there is significance difference (p < 0,05) of bax/bcl-2 expression between granulosa cells of endometriosis patients and granulosa cells in women without endometriosis.