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Abstrak :
Penelitian ini merupakan penelitian potong lintang yang bertujuan mengetahui korelasi antara kadar seng serum dengan aktivitas SOD eritrosit pada pasien geriatri non-frail dan frail di Poliklinik Geriatri RSUPN Cipto Mangunkusumo Jakarta pada pertengahan bulan Agustus 2014 sampai pertengahan bulan September 2014. Subjek didapatkan secara consecutive sampling, sebanyak 60 orang (30 orang untuk masing-masing kelompok non-frail dan frail) yang memenuhi kriteria penelitian dan mengikuti penelitian sampai selesai. Tidak didapatkan korelasi signifikan antara kadar seng serum dengan aktivitas SOD eritrosit subjek penelitian, baik secara keseluruhan maupun per kelompok. ......This cross-sectional study aimed to know the correlation between serum zinc level and the erythrocyte SOD activity both in non-frail and frail geriatric patients. These subjects were collected from mid-August 2014 to mid-September 2014 from the clinic of Geriatric, Cipto Mangunkusumo General Hospital. Through conducting a consecutive sampling, 60 subjects who met the study criteria and completed all study progress, that consisted 30 persons who represent each non- frail and frail group, were enrolled into this study. Serum zinc level did not show significant correlation with erythrocyte SOD activity, both overall and per group.

Abstrak :
Latar Belakang: Sampai saat ini belum ada terapi yang digunakan untuk mencegah iron overload pada pasien talasemia. Studi terdahulu menunjukkan bahwa ekstrak daun Mangifera foetida L. dapat menurunkan kadar besi pada model iron overload in vitro dan in vivo. Penelitian ini bertujuan untuk mengetahui efikasi ekstrak daun Mangifera foetida L. dalam mencegah terjadinya iron overload pada tikus yang diinduksi besi. Metode: Tiga puluh tikus Sprague-Dawley jantan dibagi menjadi 5 kelompok yaitu kelompok normal (tidak diberi perlakuan), kelompok iron overload (IO) dan kelompok dosis setara mangiferin (DSM) 50,100, dan 200 mg/kg BB. Kelompok IO, DSM 50, DSM 100, dan DSM 200 diberikan bersama dengan induksi Fe dekstran secara intraperitonial 15 mg seminggu dua kali selama 4 minggu. Sebelum dan sesudah 4 minggu percobaan hewan coba diambil darah dan urinnya. Setelah 4 minggu hewan coba diterminasi dan diambil organ limpa, hati, dan jantung. Pemeriksaan yang dilakukan adalah aktivitas SOD plasma, Fe urin, Fe limpa, Fe plasma, kadar mangiferin darah, dan kadar ferritin darah. Hasil: Ekstrak daun Mangifera foetida L. tidak dapat mencegah kenaikan Fe di plasma, dan limpa. Terjadi penurunan aktivitas SOD, yang disertai dengan peningkatan konsentrasi ferritin. Kesimpulan: Ekstrak daun Mangifera foetida L. tidak terbukti dapat mencegah peningkatan kadar besi, ferritin dan penurunan aktivitas antioksidan pada tikus yang diinduksi besi.

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Introduction: Presently, there is no available agent for the prevention of iron overload in thalassemia patients. Previous studies had shown that Mangifera foetida L. leaves extract reduced the levels of iron in iron overload in vitro and in vivo models. The present study aimed to determine the efficacy of Mangifera foetida L. leaves extract in the prevention of iron overload in the rats induced with iron. Methods: Thirty male Sprague-Dawley rats were divided into 5 groups treated with: none (untreated), iron overload (IO), equivalent dose group mangiferin (DSM) 50, DSM 100 and DSM 200 mg / kg BB. Fe dextran 15 mg intraperitoneal twice weekly for 4 weeks were given together with IO group, DSM 50, DSM 100 and DSM 200. Urine and blood samples were taken before and after treatments. After 4 weeks of treatment, rats were terminated and samples of spleen, liver, and heart were taken. SOD activities were done in plasma, Levels of Fe were determined in plasma, urine and spleen, while Ferritin and mangiferin levels were determined from plasma. Results: Mangifera foetida L. leaves extract did not prevent the increase of Fe plasma, and spleen. SOD activities were shown to decrease, along with the increase of ferritin concentrations. Conclusion: Mangifera foetida L. leaves extract could not prevent the increased levels of iron, ferritin and decreased antioxidant activity in rats induced by iron.