Hasil Pencarian  ::  Simpan CSV :: Kembali

"T-box 15 (Tbx15) merupakan faktor transkripsi yang mempengaruhi ekspresi gen Uncoupling protein 1 (Ucp1). Interaksi antara protein Tbx15 dan gen Ucp1 telah dilaporakan terlibat dalam aktivasi termogenesis adaptif dan pencoklatan jaringan adiposa putih, yang menawarkan pendekatan baru dalam mengobati obesitas. Paparan static magnetic field (SMF) meningkatkan Ca2+ sitosol yang dapat menghambat adipogenesis in vitro. Studi eksperimental untuk menganalisis efek SMF pada pencoklatan belum banyak dilaporkan. Oleh karena itu, penelitian ini bertujuan untuk menganalisis efek pajanan SMF secara in vivo pada jaringan adiposa mencit obesitas yang ditinjau dari ekspresi protein Tbx15, gen Ucp1, serta ukuran sel adiposa inguinal. Mencit C57BL/6J diinduksi obesitas melalui pemberian pakan tinggi lemak (HFD). Mencit dipajan SMF selama 21 hari diferensiasi adiposit, durasi pajanan 1 jam/hari dengan intensitas 2 mT. Indeks Lee, protein Tbx15, gen Ucp1 dan ukuran sel adiposa dianalisis. Ekspresi Tbx15 meningkat secara signifikan (p<0,05) setelah 2 dan 7 hari pajanan SMF dan indeks Lee menurun sejak 2-21 hari pajanan SMF. Ekspresi gen Ucp1 meningkat setelah pajanan SMF walaupun secara statistik tidak ada perbedaan signifikan. Ukuran sel adiposa lebih kecil setelah 14-21 hari pajanan. Oleh karena itu, pajanan SMF dengan intensitas 2 mT durasi 1 jam/hari sudah optimal mempengaruhi proses pencoklatan melalui ekspresi Tbx15 dan Ucp1 yang meningkat setelah 2-7 hari pajanan dan secara fenotip ukuran sel adiposa mengecil dihari 14-21.

---

T-box 15 (Tbx 15) is a transcription factor that regulates the expression of the Uncoupling protein 1(Ucp1) gene. Tbx15 protein and Ucp1 gene interaction has been reported to be involved in thermogenesis and browning process of white adipose tissue, which offers a novel approach to treat obesity. Increased Ca2+ cytosolic concentrations caused by static magnetic field (SMF) exposure inhibit adipogenesis in vitro. Experimental studies to determine effect of SMF on the browning process have not been widely reported. Hence, we investigated its effect towards Lee index, Tbx15 and Ucp1 expression, as well as adipose cell size in obese mice inguinal adipose tissue. We generated C57BL/6J obese mice by inducing high fat diet (HFD). Mice were exposed to SMF at a 2 mT intensity for one hour per day for 21 days of adipocyte differentiation. Lee index, Tbx15 protein, Ucp1 gene, and histological inguinal adipose histology were all investigated. Tbx15 expression increased after 2-7 days of SMF exposure and Lee index decreased significantly since 2- 21 days of SMF exposure. Ucp1 gene expression increased after SMF exposure, however there was no significant change following SMF exposure. After 14-21 days of exposure, adipose cell size was slightly reduced. Therefore, we can conclude that the SMF exposure at 2 mT intensity for one hour per day could improve browning process by increasing Tbx15 dan Ucp1 expression after 2-7 days and adipose cell size phenotypically reduced at 14-21 days of SMF exposure."

"ABSTRAKOverweight dan obesitas adalah akumulasi lemak yang berlebihan yang dapat mengganggu kesehatan. Obesitas merupakan salah satu risiko untuk terjadinya gangguan kardiometabolik. Adanya polimorfisme gen UCP1, menyebabkan bervariasinya respons terhadap olahraga teratur SALI.Penelitian ini bertujuan menganalisis pengaruh senam aerobik low impact SALI pada perempuan obes abdominal yang memiliki polimorfisme gen UCP1 terhadap parameter kardiometabolik lingkar pinggang LP , kadar trigliserida TG dan penanda inflamasi Monocyte Chemmoattractant Protein-1 MCP1 .Desain penelitian adalah non randomized controlled trial. Intervensi 12 minggu terhadap 55 orang wanita obes abdominal, terdiri dari 32 orang kelompok intervensi SALI dan 23 orang kelompok kontrol. Sebelum dan sesudah program dilakukan pemeriksaan parameter kardiometabolik LP, kadar TG dan MCP1. Pemeriksaan polimorfisme -3826 A>G gen UCP1 menggunakan teknik PCR diikuti teknik RFLP.Ditemukan frekuensi genotip AA 21 orang 38,2 , genotip AG 27 49,1 dan genotip GG 7 12,7 , dengan frekuensi alel G 0,40 . Subjek dengan kadar TG ge; 130 mg/dL kelompok SALI 100 responders, Kontrol 55 , dan kelompok kadar TG < 130 mg/dL, 22 . Subjek genotip GG polimorfisme gen UCP1 dengan kadar TG ge; 130 mg/dL high responders. genotip AA low responders. Temuan ini diperkirakan terkait jumlah langkah per hari kelompok genotip GG dengan TG ge; 130 mg/dL lebih tinggi dibandingkan kelompok nonresponders.

---

ABSTRACTOverweight and obesity were the accumulation of excessive fat that could harm health. Obesity was a risk for cardiometabolic disorders. The polymorhism of UCP1 gene, caused variations in response to regular exercise. This study aims to investigate the influence of low impact aerobics exercise LIAE in abdominal obes women who had the polymorphism of UCP1 gene on cardiometabolic parameters, waist circumference WC , levels of triglyceride TG and inflammatory markers Monocyte Chemoattractant Protein 1 MCP1 . The study design was non randomized controlled trial. A total of 55 women subjects moderately obes women were divided into two groups on the basis of location. Thirty two subjects of LIAE group and 23 a non LIAE control group. Subjects were not restricted in foods consumed. The study period was 12 weeks. Outcome assessments for analyses were completed at baseline and 12 weeks for cardiometabolic parameters WC, TG and MCP1. Examination of the polymorphism 3826 A G UCP1 gene using PCR technique followed by RFLP technique. The frequencies of three genotypes of 3826 A G polymorphism of UCP1 gene were AA, AG, and GG were of 21 38.2 , 27 49.1 and 7 12.7 respectively with the G allele frequency of 0.40 . Post study obtained the subjects with TG baseline TG ge 130 mg dL 100 LIAE responders group, Control 55 , while TG "