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Abstrak :

Penelitian ini bertujuan untuk memperoleh informasi mengenai ekologi di tiga lokasi yang berbeda, keanekaragaman morfologi, molekuler dan struktur alginat yang dihasilkan. Pengambilan sampel  menggunakan metode transek. Analisis data menggunakan analisis Statistik R. Analisis pohon filogeni menggunakan metode Maximum Likelihood (ML) berdasarkan marker ITS2, rbcL dan COX3 boostrap untuk 1000 ulangan >50%. Isolasi alginat dilakukan melalui hidrolisis parsial dilanjutkan dengan analisis kualitatif dan kuantitatif menggunakan FTIR. Hasil penelitian menunjukkan indikator salinitas sebesar 36 ‰, DO 4,74 dan suhu 29,00 ^oC menjadi ciri khas lokasi Binuangeun. Hasil analisis statistik pada morfometri menunjukkan indikator Pulau Lima memiliki ciri khas morfometri dengan indikator panjang total tertinggi. Binuangeun dan Ujung Kulon memiliki komponen berkebalikan yaitu lebar daun, panjang daun, batang utama dan vesikel. Binuangeun memiliki diameter talus yang lebih besar dibandingkan Ujung Kulon dan Pulau Lima. Hasil analisis filogenetik memperlihatkan adanya tiga buah klad dengan dukungan bootstrap >50%. Hasil uji hidrolisis alginat parsial menunjukkan alginat di Pulau Lima, Ujung Kulon dan Binuangeun konsentrasi M/G % adalah 1,35 %, 1,44 % dan 2,33 %. Kesimpulan penelitian ini adalah lokasi Binuangeun di pantai Samudera Indonesia merupakan lokasi ekologi terbaik bagi habitat S. polycystum untuk mendapatkan alginat yang memenuhi persyaratan kualitas bahan baku industri pangan dan non pangan.

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The study aim was to obtain information on ecology in three different locations, the morphology, molecular and alginate characters from Sargassum polycystum. The sampling technique was using the transect methode. Data analysis using R statistic analysis. Phylogeny tree analysis was using the Maximum Likelihood (ML) method based on ITS2, rbcLS and COX3 bootstrap alignment for 1000 replications. Isolation of alginate was conducted through partial hydrolysis, the parameters were measured qualitatively and quantitatively using FTIR. The results showed that the highest salinity 36 ^o/_oo, DO 4,74 and the lowest temperature 29,00^oC as characteristic indicators of the Binuangeun location. The results of the morphometric analysis showed that the indicator of Pulau Lima with the highest total length indicator, Binuangeun and Ujung kulon have the opposite component. The results of phylogenetic analysis tree based on ITS-2, rbcL and COX3 marker showed that there are three clades supported by bootstrap 1000 >50%. The results of the partial alginate hydrolysis test showed that concentration of alginate on the Lima Island, Ujung Kulon and Binuangeun M/G % were 1.35 %, 1.44 % and 2.33 %, respectively.The conclusion is Binuangeun is a good ecological location for the growth habitat of S. polycystum for food and non-food industries.

Abstrak :
Kanker paru merupakan salah satu kanker yang memiliki angka mortalitas yang tinggi di Indonesia. Sargassum polycystum merupakan salah satu spesies rumput laut yang banyak ditemui di perairan laut Indonesia dan memiliki potensi sebagai agen antikanker namun belum banyak diteliti. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder dan aktivitas sitotoksik dari ekstrak Sargassum polycystum terhadap sel kanker paru A-549. Sargassum polycystum diekstraksi menggunakan empat jenis pelarut, sehingga menghasilkan empat jenis ekstrak Sargassum polycystum, yaitu ekstrak n-heksana, ekstrak etil asetat, ekstrak kloroform, dan ekstrak etanol. Uji fitokimia pada keempat ekstrak Sargassum polycystum menunjukkan metabolit sekunder berupa flavonoid, tannin, glikosida, alkaloid, triterpenoid, dan steroid. Pada uji KLT menunjukkan bahwa ekstrak Sargassum polycystum memiliki tiga hingga empat senyawa kimia. Uji in vitro aktivitas sitotoksik keempat ekstrak Sargassum polycystum terhadap sel kanker paru A549 menggunakan metode MTT assay pada panjang gelombang 492 nm dengan 5 variasi konsentrasi, yaitu 3,125 g/mL; 6,25 g/mL; 12,5 g/mL; 25 g/mL; dan 50 g/mL. Data yang diperoleh dianalisis dengan menggunakan microsoft excel sehingga diperoleh nilai IC50. Berdasarkan nilai IC50 yang diperoleh, semua ekstrak memiliki nilai IC500,05. Hal ini menunjukkan ekstrak n-heksana memiliki aktivitas sitotoksik paling kuat terhadap sel kanker paru A-549 dibandingkan ekstrak lainnya, sehingga ekstrak n-heksana Sargassum polycystum berpotensi dikembangkan sebagai agen antikanker paru.

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Lung cancer is one of cancer that has high mortality rate in Indonesia. Sargassum polycystum is one of macroalgae species that commonly found in Indonesian marine and can be developed as anticancer agent but has not been widely studied. Objective of this research is to know second metabolites compounds and cytotoxic activity of extract Sargassum polycyctum on A 549 lung cancer cell. Sargassum polycystum was extracted using four solvent to produce four extract, there are n hexane extract, ethyl acetate extract, chloroform extract, and ethanol extract. Phytochemical test of each extract showed positive result of flavonoid, tannin, glycoside, alkaloid, triterpenoid, and steroid. Besides, TLC test showed there were three to four chemical compound in each extract of Sargassum polycystum. In vitro cytotoxic activity of four extract Sargassum polycystum on A 549 lung cancer were tested using MTT assay on 492 nm wavelength and devided into five different concentration, there are 3,125 g ml 6,25 g ml 12,5 g ml 25 g ml and 50 g ml. The obtained data were analyzed using microsoft excel to get IC50 value. The result showed that each extract of Sargassum polycycstum had IC50 values less than 100 g ml. This means each extract of Sargassum polcystum has cytotoxic activity against A 549 lung cancer cell. Among four extract of Sargassum polycytum, n hexane extract has the lowest IC50 value 24,1 g ml and shows the most potent cytotoxic activity against A 549 lung cancer cell. Statistical analytic showed p value 0,05. So, n hexane extract of Sargassum polycystum potential to be developed as antilung cancer agent.

Abstrak :
[ABSTRAK
Penelitian mengenai potensi makroalga alami Sargassum polycystum dan makroalga budidaya Eucheuma cottonii dalam menyerap dan menyimpan karbon serta nutrien di Pulau Panjang, Teluk Banten telah dilakukan pada bulan Oktober hingga November 2014. Penelitian bertujuan untuk mengetahui laju penyerapan karbon, kandungan nutrien dan produktivitas primer dari makroalga S. polycystum dan E. cottonii. Lokasi penelitian terletak di bagian hamparan gosong karang dan berlumpur dengan kedalaman 0,5-5 m. Pengamatan pertumbuhan dan laju penyerapan karbon menggunakan metode penandaan thallus pada 30 sampel makroalga setiap hari selama 7 hari. Sampel makroalga selanjutnya dianalisis kandungan nutriennya. Hasil penelitian didapatkan estimasi laju penyerapan karbon S. polycystum dan E. cottonii adalah 0,0081 gC/hari dan 0,0083 gC/hari. Kandungan karbon, nitrogen dan fosfat di S. polycystum adalah sebanyak 6,84%, 1,72% and 0,009% sedangkan kadar karbon, nitrogen dan fosfat di E. cottonii adalah 5,99%, 0,67% dan 0,006%. Berdasarkan analisis statistik dengan uji t, terdapat perbedaan yang signifikan pada laju pertumbuhan, kandungan nitrogen dan fosfat S. polycystum dengan E. cottonii. Sedangkan untuk kandungan karbon tidak terdapat perbedaan yang signifikan antara S. polycystum dengan E. cottonii. Selanjutnya, uji korelasi didapatkan bahwa adanya korelasi antara laju pertumbuhan S. polycystum dan E. cottonii dengan kandungan nitrogen masing- masing sedangkan antara laju pertumbuhan dengan kandungan karbon dan fosfat tidak terdapat korelasi. Produktivitas primer diukur dengan metode botol terang dan botol gelap yang dimodifikasi. Pengukuran produktivitas primer dilakukan pada kedalaman 0,5 m untuk S. polycystum dan 0,1 m untuk E. cottonii. Kandungan oksigen terlarut diukur dengan DO meter. Hasil penelitian didapatkan rata-rata produktivitas primer S. polycystum sebesar 512,99 ± 169,26 mgC/m3/hari dan E. cottonii sebesar 387,88 ± 219,93 mgC/m3/hari.

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ABSTRAK
The research about the potency of wild macroalgae Sargassum polycystum dan cultivation macroalgae Eucheuma cottonii to absorb and store carbon also nutrient in Panjang Island, Banten Bay was held on October until November 2014. The research was aimed to estimate carbon sequestration, nutrient content and primary productivity from macroalgae S. polycystum and E. cottonii. Location of the research in the stretch of reef and muddy with a depth of 0,5-5 m. The observation of growth rate and carbon sequestration used thallus marking method in 30 macroalgae shoots everyday for 7 days. Macroalgae was analyzed its nutrient content. The results showed that estimation carbon sequestration by S. polycystum and E. cottonii were 0,0081 gC/day dan 0,0083 gC/day. The content of carbon, nitrogent and phosphor for Macroalgae S. polycystum were 6,84%, 1,72% and 0,009% respectively while the content of carbon, nitrogent and phosphor for Macroalgae E. cottonii were 5,99%, 0,67% dan 0,006% respectively. Based on statistical analysis by t test, there was found significant difference on the growth rate, nitrogent and phosphor content of S. polycystum with E. cottonii. While for the carbon content, there was no significant difference between S. polycystum with E. cottonii. Furthermore, correlation test showed that there was a correlation between the growth rate of S. polycystum and E. cottonii with nitrogent content respectively while between the growth rate with carbon and phosphor content, there was no correlation. Primary productivity were measured by the light and dark bottle method with modification. Measurement of primary productivity was held at a depth of 0,5 m from the surface for S. polycystum and 0,1 m from the surface for E. cottonii. Dissolved oxygen was measured by DO meter. The result showed that mean of primary productivity for S. polycystum was 512,99 ± 169,26 mgC/m3/day and E. cottonii was 387,88 ± 219,93 mgC/m3/day.;The research about the potency of wild macroalgae Sargassum polycystum dan cultivation macroalgae Eucheuma cottonii to absorb and store carbon also nutrient in Panjang Island, Banten Bay was held on October until November 2014. The research was aimed to estimate carbon sequestration, nutrient content and primary productivity from macroalgae S. polycystum and E. cottonii. Location of the research in the stretch of reef and muddy with a depth of 0,5-5 m. The observation of growth rate and carbon sequestration used thallus marking method in 30 macroalgae shoots everyday for 7 days. Macroalgae was analyzed its nutrient content. The results showed that estimation carbon sequestration by S. polycystum and E. cottonii were 0,0081 gC/day dan 0,0083 gC/day. The content of carbon, nitrogent and phosphor for Macroalgae S. polycystum were 6,84%, 1,72% and 0,009% respectively while the content of carbon, nitrogent and phosphor for Macroalgae E. cottonii were 5,99%, 0,67% dan 0,006% respectively. Based on statistical analysis by t test, there was found significant difference on the growth rate, nitrogent and phosphor content of S. polycystum with E. cottonii. While for the carbon content, there was no significant difference between S. polycystum with E. cottonii. Furthermore, correlation test showed that there was a correlation between the growth rate of S. polycystum and E. cottonii with nitrogent content respectively while between the growth rate with carbon and phosphor content, there was no correlation. Primary productivity were measured by the light and dark bottle method with modification. Measurement of primary productivity was held at a depth of 0,5 m from the surface for S. polycystum and 0,1 m from the surface for E. cottonii. Dissolved oxygen was measured by DO meter. The result showed that mean of primary productivity for S. polycystum was 512,99 ± 169,26 mgC/m3/day and E. cottonii was 387,88 ± 219,93 mgC/m3/day.;The research about the potency of wild macroalgae Sargassum polycystum dan cultivation macroalgae Eucheuma cottonii to absorb and store carbon also nutrient in Panjang Island, Banten Bay was held on October until November 2014. The research was aimed to estimate carbon sequestration, nutrient content and primary productivity from macroalgae S. polycystum and E. cottonii. Location of the research in the stretch of reef and muddy with a depth of 0,5-5 m. The observation of growth rate and carbon sequestration used thallus marking method in 30 macroalgae shoots everyday for 7 days. Macroalgae was analyzed its nutrient content. The results showed that estimation carbon sequestration by S. polycystum and E. cottonii were 0,0081 gC/day dan 0,0083 gC/day. The content of carbon, nitrogent and phosphor for Macroalgae S. polycystum were 6,84%, 1,72% and 0,009% respectively while the content of carbon, nitrogent and phosphor for Macroalgae E. cottonii were 5,99%, 0,67% dan 0,006% respectively. Based on statistical analysis by t test, there was found significant difference on the growth rate, nitrogent and phosphor content of S. polycystum with E. cottonii. While for the carbon content, there was no significant difference between S. polycystum with E. cottonii. Furthermore, correlation test showed that there was a correlation between the growth rate of S. polycystum and E. cottonii with nitrogent content respectively while between the growth rate with carbon and phosphor content, there was no correlation. Primary productivity were measured by the light and dark bottle method with modification. Measurement of primary productivity was held at a depth of 0,5 m from the surface for S. polycystum and 0,1 m from the surface for E. cottonii. Dissolved oxygen was measured by DO meter. The result showed that mean of primary productivity for S. polycystum was 512,99 ± 169,26 mgC/m3/day and E. cottonii was 387,88 ± 219,93 mgC/m3/day., The research about the potency of wild macroalgae Sargassum polycystum dan cultivation macroalgae Eucheuma cottonii to absorb and store carbon also nutrient in Panjang Island, Banten Bay was held on October until November 2014. The research was aimed to estimate carbon sequestration, nutrient content and primary productivity from macroalgae S. polycystum and E. cottonii. Location of the research in the stretch of reef and muddy with a depth of 0,5-5 m. The observation of growth rate and carbon sequestration used thallus marking method in 30 macroalgae shoots everyday for 7 days. Macroalgae was analyzed its nutrient content. The results showed that estimation carbon sequestration by S. polycystum and E. cottonii were 0,0081 gC/day dan 0,0083 gC/day. The content of carbon, nitrogent and phosphor for Macroalgae S. polycystum were 6,84%, 1,72% and 0,009% respectively while the content of carbon, nitrogent and phosphor for Macroalgae E. cottonii were 5,99%, 0,67% dan 0,006% respectively. Based on statistical analysis by t test, there was found significant difference on the growth rate, nitrogent and phosphor content of S. polycystum with E. cottonii. While for the carbon content, there was no significant difference between S. polycystum with E. cottonii. Furthermore, correlation test showed that there was a correlation between the growth rate of S. polycystum and E. cottonii with nitrogent content respectively while between the growth rate with carbon and phosphor content, there was no correlation. Primary productivity were measured by the light and dark bottle method with modification. Measurement of primary productivity was held at a depth of 0,5 m from the surface for S. polycystum and 0,1 m from the surface for E. cottonii. Dissolved oxygen was measured by DO meter. The result showed that mean of primary productivity for S. polycystum was 512,99 ± 169,26 mgC/m3/day and E. cottonii was 387,88 ± 219,93 mgC/m3/day.]

Abstrak :
Sargassum polycystum diketahui secara in vitro memiliki aktivitas sitotoksik terhadap sel HCT-116 pada kolon yang diuji pada beberapa pelarut dan dengan nilai IC50 yang berbeda. Namun, penelitian secara in vivo Sargassum polycystum pada kanker kolon belum banyak dilakukan dan mekanisme sepenuhnya dalam penghambatan kanker belum diketahui. Penelitian ini bertujuan untuk mengetahui efek alga coklat (Sargassum polycystum) secara in vivo pada hewan model kolitis terkait kanker kolon yang diinduksi dengan Dekstran Sodium Sulfat (DSS). Penelitian ini menggunakan mencit jantan galur Balb/c (n = 30 ekor) yang secara acak dibagi dalam 5 kelompok: kelompok normal, kelompok negatif, kelompok dosis 1 (18 mg/kgBB), kelompok dosis 2 (90 mg/kgBB), dan kelompok dosis 3 (450 mg/kgBB). Induksi kolitis terkait kanker kolon menggunakan senyawa kimia Dekstran Sodium Sulfat (DSS) dengan konsentrasi 2% dan 1% selama total 24 hari. Pengukuran berat badan, analisis kelangsungan hidup, dan penilaian Disease Activity Index (DAI) dilakukan selama penelitian berlangsung. Pengaruh Sargassum polycystum sebagai antikanker diamati dengan memeriksa variabel inflamasi IL-1β dan pemeriksaan histologi jaringan kolon dengan periodic acid-schiff (PAS). Hasil penelitian menunjukkan bahwa hewan uji yang diberikan Sargassum polycystum pada dosis 18 mg/kgBB memiliki kelangsungan hidup lebih tinggi dan dapat menurunkan ekspresi variabel inflamasi IL-1β. ......Sargassum polycystum is known in vitro to have cytotoxic activity against HCT-116 cells in the colon, which were tested in several solvents with different IC50 values. However, in vivo studies of Sargassum polycystum on colon cancer have not been widely carried out, and the full mechanism of cancer inhibition is not yet known. This study aims to determine the effect of brown algae (Sargassum polycystum) in vivo on an animal model of colitis related to colon cancer induced by Dextran Sodium Sulfate (DSS). This study used male mice of the Balb/c strain (n = 30), which were randomly divided into 5 groups: normal group, negative group, dose 1 group (18 mg/kgBW), dose 2 group (90 mg/kgBW), and dose group 3 (450 mg/kgBW). Induction of colitis-associated colon cancer using the chemical compound Dextran Sodium Sulfate (DSS) with a concentration of 2% and 1% for a total of 24 days. Body weight measurements, survival analysis, and Disease Activity Index (DAI) assessments were carried out during the study. The effect of Sargassum polycystum as an anticancer agent was observed by examining the inflammatory variable IL-1β and histological examination of colonic tissue with periodic acid-schiff (PAS). The results showed that the test animals that were given Sargassum polycystum at a dose of 18 mg/kgBW had higher survival and could reduce the expression of the inflammatory variable IL-1β.

Abstrak :
Alga coklat (S. polycystum) yang diekstraksi dengan berbagai macam pelarut diketahui memiliki aktivitas sitotoksik pada sel kanker kolon HCT-116. Nilai IC-50 paling baik ditunjukkan pada alga coklat (S. polycystum) yang diekstraksi menggunakan pelarut etil asetat. Penelitian ini bertujuan untuk membuktikan efek ekstrak alga coklat (S. polycystum) dalam menghambat kolitis terkait kanker kolon secara in vivo. Sebanyak 30 ekor mencit jantan galur Balb/C dibagi acak dalam 5 kelompok yaitu normal, negatif, dosis 1 (18 mg/kgBB), dosis 2 (90 mg/kgBB), dosis 3 (450 mg/kgBB). Hewan model dibuat dengan mengadministrasikan Dekstran Sodium Sulfat (DSS) selama 24 hari secara berturut-turut dengan konsentrasi 2% selama 7 hari, konsentrasi 1% selama 10 hari, dan konsentrasi 2% selama 7 hari. Pemberian ekstrak alga coklat (S. polycystum) diberikan pada hari ke-8 berlanjut hingga 14 hari setelah induksi. Selama penelitian berlangsung dilakukan pengukuran berat badan hewan, kemampuan bertahan hidup, dan penilaian Disease Activity Index (DAI). Setelah pengorbanan hewan dilakukan isolasi kolon untuk mengukur panjang, berat kolon, kerusakan jaringan, jumlah sel goblet dengan metode histopatologi kolon serta analisis ekspresi Caspase-3 pada jaringan kolon menggunakan metode ELISA. Hasil penelitian menunjukkan bahwa ekstrak alga coklat (S. polycystum) pada dosis 1 (18 mg/kgBB), menunjukkan perbaikan kerusakan jaringan kolon paling optimal dan memiliki konsentrasi Caspase-3 paling tinggi dibandingkan kelompok dosis lainnya. ......Brown algae (S. polycystum) extracted with various solvents is known to have cytotoxic activity on HCT-116 colon cancer cells. IC-50 value is best shown in brown algae (S. polycystum) extracted using ethyl acetate solvent. This study aims to prove the effect of brown algae extract (S. polycystum) in inhibiting colitis associated with colon cancer in vivo. Thirty male mice Balb/C strain were randomly divided into 5 groups: normal, negative, dose 1 (18 mg/kgBB), dose 2 (90 mg/kgBB), dose 3 (450 mg/kgBB). Animal models were made by administering Dextran Sodium Sulphate (DSS) for 24 consecutive days with a concentration of 2% for 7 days, 1% concentration for 10 days, and 2% concentration for 7 days. Brown algae extract (S. polycystum) was given on the 8th day and continued until 14 days after induction. During the study, animal body weight, survival ability, and Disease Activity Index (DAI) were measured. After animal sacrifice, colon isolation was carried out to measure the length, weight of the colon, tissue damage, number of goblet cells using the colon histopathology method and analysis of Caspase-3 expression in colon tissue using the ELISA method. The results showed that brown algae extract (S. polycystum) at dose 1 (1.8 mg/kg BW) showed the most optimal colonic damage improvement and had the highest concentration of Caspase-3 compared to the other dose groups.