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Abstrak :
Dua puluh lima ekor tikus dibagi dalam lima kelompok perlakuan, terdiri atas kelompok normal kontrol (KK1) yang tidak diberi diet tinggi lemak dan kolesterol, kelompok perlakuan kontrol (KK2) yang diberi diet tinggi lemak dan kolesterol, dan tiga kelompok perlakuan (KP1, KP2, dan KP3) yang diberi diet tinggi lemak dan kolesterol dan suspensi biomassa Rhodotorula minuta UICC Y- 227 dengan dosis 5, 10, dan 20 mg/kg bb. Pemberian bahan uji dilakukan setiap hari selama 21 hari berturut-turut. Pengambilan sampel darah dilakukan pada hari ke-0 dan hari ke-22, kemudian dilakukan analisis konsentrasi trigliserida berdasarkan hasil reaksi glycerol phosphate oxidase (GPO). Rerata nilai konsentrasi trigliserida secara berturut-turut pada KK1, KK2, KP1, KP2, dan KP3 adalah 80,25 mg/dl ± 0,85; 87,49 mg/dl ± 1,93; 73,48 mg/dl ± 1,71; 72,72 mg/dl ± 1,83; dan 68,61 mg/dl ± 0,84. Berdasarkan uji LSD (P < 0,05) menunjukkan adanya penurunan konsentrasi trigliserida pada seluruh kelompok dosis. Rerata penurunan konsentrasi trigliserida hingga di bawah konsentrasi trigliserida kelompok normal kontrol dicapai oleh kelompok perlakuan dengan dosis 20 mg/kg bb. Sedangkan, kelompok perlakuan dengan dosis 5 dan 10 mg/kg bb mendekati konsentrasi trigliserida kelompok normal kontrol.

Abstrak :
Tikus merupakan salah satu binatang yang bisa menularkan penyakit. Berbagai  macam pengendalian tikus dilakukan oleh masyarakat. Salah satu cara pengendaliannya menggunakan kapur barus. Penelitian ini bertujuan untuk mengetahui efek kapur barus terhadap konsumsi pakan tikus Rattus norvegicus galur wistar. Jenis penelitian yang digunakan adalah penelitian quasi eksperimen dengan menggunakan kapur barus yang diletakkan berdekatan dengan tempat makan tikus yang kemudian dilihat rata-rata konsumsi pakan selama tiga hari. Hasil penelitian menunjukkan tidak ada perbedaan penggunaan kapur barus terhadap tikus. Hal ini dikarenakan kapur barus yang berupa padatan bersifat lebih sulit atau menguap pada area terbuka sehingga tidak terlalu menyengat indra  penciuman tikus.

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Rats are animals that can transmit diseases. Various kinds of rat control are carried out by the community. One way to control it uses moth balls. This study aims to determine the effect of camphor on consumption of  Rattus norvegicus wistar strain rats feed. This type of research is a quasi experimental study using mothballs which are placed adjacent to a rat's place to eat and then see the average feed consumption for three days. The results of this study showed that there was no difference in the use of moth balls on rat. This is because moth balls in the form of solids are more difficult or evaporate in an open area so they do not overpower the rat's sense of smell.

Abstrak :
Suplemen kalsium dapat membantu mencegah kasus osteopenia dan osteoporosis, selain itu juga dapat digunakan dalam pengobatan bersama-sama dengan obat lain. Penelitian ini membahas metode-metode pengukuran yang tepat secara fisika biomedis dari tulang tikus putih Rattus norvegicus yang diovariektomi dan diberi perlakuan diet nano kalsium fosfat. Tujuannya yaitu untuk mendapatkan metode yang tepat dalam mendeteksi status penulangan kembali, dari hewan model yang mendapat diet nano kalsium fosfat dalam perbaikan tulang osteoporosis pascaovariektomi (pasca-OVX). Penelitian ini merupakan penelitian kualitatif dan kuantitatif yang dilakukan dalam serangkaian penelitian dan dibagi dalam tiga prosedur kerja. Diet dibuat dalam tiga jenis yaitu A (diet dengan nano kalsium 0,1%), B (diet dengan nano kalsium 0,4%) dan C (diet dengan nano kalsium 0,7%). Parameter yang diukur pada tahap pertama yaitu kandungan nutrisi dan mineral diet. Tahap kedua yaitu operasi OVX pada tikus dan dipelihara normal untuk mengkondisikan tikus osteoporosis. Parameter yang diukur pada tahap kedua yaitu mineral serum dan tulang, gugus fungsional tulang, morfologi, struktur kristal dan densitas tulang tibia dan femur yang dilakukan setiap dua minggu saat proses osteoporosis. Tahap ketiga yaitu tahap perlakuan pemberian diet nano kalsium fosfat terhadap tikus osteoporosis akibat OVX. Parameter yang dianalisis yaitu konsumsi bahan kering, konsumsi kalsium, kalsium dalam feses, persentase serapan kalsium, kandungan kalsium, magnesium dan fosfor dalam serum, tulang femur dan tibia, gugus fungsional tulang, morfologi, struktur kristal dan densitas tulang femur dan tibia pada saat proses recovery. Alat karakterisasi yang digunakan yaitu Transmission Electron Microscopy (TEM), Atomic Absorption Spectroscopy (AAS), Ultraviolet-Visible (Uv-Vis), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy/Energy Dispersive Spectroscopy (SEM/EDS), X-ray Diffraction (XRD) dan Computed Tomography (CT) Scan. Analisis data yang digunakan adalah uji-t bebas, dengan membandingkan hasil yang diperoleh antara tikus non-OVX dan OVX serta membandingkan hasil dari tikus yang diberi diet A dengan B dan B dengan C. Hubungan antara metode deteksi dengan data biologis diuji dengan analisis regresi berganda. Berdasarkan data yang diperoleh, hasil riset tahap satu menginformasikan bahwa kandungan nutrisi dan mineral diet sesuai dengan standar diet yang direkomendasikan oleh National Research Council (NRC) USA. Hasil riset tahap dua yaitu tikus yang diovariektomi menunjukkan tanda osteoporosis dengan menurunnya kadar kalsium dalam serum, tulang femur dan tibia, terjadinya penurunan ion fosfat dan densitas elektron, serta ukuran butir yang lebih besar terjadi pada minggu ke-7 sejak OVX. Hasil perlakuan ketiga jenis diet nano kalsium fosfat pada riset tahap tiga menunjukkan bahwa tikus osteoporosis pasca-OVX yang diberi diet dengan nano kalsium 0,4% memberikan hasil yang lebih efektif dan efisien dibandingkan dengan tikus osteoporosis pasca-OVX yang diberi diet dengan kandungan nano kalsium 0,1% dan 0,7%.

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Calcium suplements are used as an aid in the prevention of osteopenia and osteoporosis, and also for the treatment of patients when used along with medication. This study analyzed precise measurements for physic-medical bones of ovariectomized white rats (Rattus norvegicus) which were conditioned in a nano calcium phosphate diet treatment. The goal was to get the right method for detecting bone rebalancing from animal models that had a nano calcium phosphate diet in postovariectomy (post-OVX) condition for osteoporosis bone repair. This research was qualitatively and quantitatively conducted in a series of studies and divided into three work procedures. The first step was the production of a nano calcium phosphate diet. Three types, namely A (diet with 0.1% nano calcium according to normal needs), B (diet with 0.4% nano calcium) and C (diet with 0.7% nano calcium). The second step was rats OVX surgery and the rats were maintained normally up to osteoporosis stage. The parameters measured in the second step were serum and bone minerals, bone functional groups, morphology, crystalline structure and density of the tibia and femur that were carried out every two weeks during the osteoporosis process. The third step involved the osteoporosis rats (ovariectmized rats) that had the nano calcium phosphate diet treatment. The parameters analyzed were dry matter consumption, calcium consumption, feces in calcium, percentage of calcium absorption, calcium, magnesium and phosphorus content in serum, femur and tibia, bone functional groups, morphology, crystal structure and femur and tibia bone density during the recovery process. The characterization were Transmission Electron Microscopy (TEM), Atomic Absorption Spectroscopy (AAS), Ultraviolet-Visible (Uv-Vis), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy / Energy Dispersive Spectroscopy (SEM / EDS), X-ray Diffraction (XRD) and Computed Tomography (CT) Scan. The free t-test was applied to analyzed the data, by comparing the results obtained between non-OVX and OVX rats and comparing the results of rats grouped diet A with B and diet B with C. The relationship between the detection method and biological data was tested by multiple regression analysis. Based on the data obtained, the first step results informed that the nutritional and mineral contents of the diet were in accordance with diet standards which were recommended by the National Research Council (NRC) USA. The second test results showed that ovariectomized rats had the signs of osteoporosis with decreasing in calcium levels in serum, femur and tibia, in phosphate ions and electron density, and increasing larger grain size occurred in the 7th week since OVX. The third step results revealed that post-OVX osteoporosis rats in the 0.4% nano calcium diet showed more effective and efficient bone-rebalancing compared to post-OVX osteoporosis rats in 0.1% or 0.7% nano calcium.

Abstrak :
Telah dilakukan penelitian untuk mengetahui pengaruh infus simplisia sirih merah (Piper betle L.) terhadap konsentrasi trigliserida plasma darah tikus putih (Rattus norvegicus) jantan galur Sprague-Dawley. Dua puluh lima ekor tikus dibagi dalam lima kelompok, terdiri atas kelompok kontrol normal yang tidak diberi diet lemak tinggi, kelompok kontrol perlakuan yang diberi diet lemak tinggi, dan tiga kelompok perlakuan yang diberi diet lemak tinggi dan infus simplia sirih merah dengan dosis 2%, 4%, dan 6% b/v. Bahan uji diberikan setiap hari selama 14 hari berturut-turut. Hasil uji Anava satu faktor (P < 0,05) menunjukkan adanya pengaruh nyata pemberian infus simplisia sirih merah terhadap konsentrasi trigliserida akhir pada semua kelompok perlakuan. Konsentrasi trigliserida pada kelompok perlakuan yang mendekati kelompok kontrol normal dicapai oleh kelompok perlakuan dengan dosis 2% b/v.

Abstrak :
[ABSTRAK
Telah dilakukan penelitian untuk mengetahui pengaruh pemberian simplisia Alphitobius sp. terhadap konsentrasi kolesterol total plasma darah tikus putih (Rattus norvegicus L.) jantan galur Sprague-Dawley. Dua puluh empat ekor tikus dibagi dalam 6 kelompok perlakuan yang terdiri atas kelompok kontrol normal yang hanya diberi pakan standar, kelompok kontrol perlakuan yang diberi diet tinggi lemak, dan 4 kelompok perlakuan yang diberi diet tinggi lemak dan bahan uji simplisia Alphitobius sp. dengan dosis 2,25 mg/kg bb, 4,5 mg/kg bb, 6,75 mg/kg bb dan 9 mg/kg bb. Pemberian bahan uji dan diet tinggi lemak dilakukan selama 14 hari berturut-turut. Hasil uji Anava satu arah (P < 0,05) menunjukkan terdapat pengaruh yang nyata terhadap konsentrasi kolesterol total akhir pada semua dosis perlakuan. Penurunan konsentrasi kolesterol terbesar dicapai oleh kelompok dosis 4,5 mg/kg bb yang juga merupakan dosis efektif dalam menurunkan konsentrasi kolesterol total yang mendekati nilai kelompok kontrol normal. ABSTRACT
A research was done in to determine the effect of simplicia Alphitobius sp. intake on plasma total cholesterol concentration in white male rat Sprague-Dawley (Rattus norvegicus L.). Twenty-four male rats were separated into six treatment groups consisting of normal control group that were given a standard diet, treatment control group fed a high fat diet , and four treatment groups that were given a high fat diet and simplicia Alphitobius sp. with a different dose (2,25 mg/kg bw, 4,5 mg/kg bw, 6,75 mg/kg bw and 9 mg/kg bw). Provision of test material was done every day for 14 consecutive days. The result of one-way Anava test (P < 0,05) indicates that there is a significant effect on total cholesterol concentration at all treatment doses, showed that provision of simplicia Alphitobius sp. lower total cholesterol potentially. The biggest decrease of total cholesterol concentrations was achieved by dose 4,5 mg/kg bw, which is also an effective dose in lowering total cholesterol concentrations approached normal value. ;A research was done in to determine the effect of simplicia Alphitobius sp. intake on plasma total cholesterol concentration in white male rat Sprague-Dawley (Rattus norvegicus L.). Twenty-four male rats were separated into six treatment groups consisting of normal control group that were given a standard diet, treatment control group fed a high fat diet , and four treatment groups that were given a high fat diet and simplicia Alphitobius sp. with a different dose (2,25 mg/kg bw, 4,5 mg/kg bw, 6,75 mg/kg bw and 9 mg/kg bw). Provision of test material was done every day for 14 consecutive days. The result of one-way Anava test (P < 0,05) indicates that there is a significant effect on total cholesterol concentration at all treatment doses, showed that provision of simplicia Alphitobius sp. lower total cholesterol potentially. The biggest decrease of total cholesterol concentrations was achieved by dose 4,5 mg/kg bw, which is also an effective dose in lowering total cholesterol concentrations approached normal value. ;A research was done in to determine the effect of simplicia Alphitobius sp. intake on plasma total cholesterol concentration in white male rat Sprague-Dawley (Rattus norvegicus L.). Twenty-four male rats were separated into six treatment groups consisting of normal control group that were given a standard diet, treatment control group fed a high fat diet , and four treatment groups that were given a high fat diet and simplicia Alphitobius sp. with a different dose (2,25 mg/kg bw, 4,5 mg/kg bw, 6,75 mg/kg bw and 9 mg/kg bw). Provision of test material was done every day for 14 consecutive days. The result of one-way Anava test (P < 0,05) indicates that there is a significant effect on total cholesterol concentration at all treatment doses, showed that provision of simplicia Alphitobius sp. lower total cholesterol potentially. The biggest decrease of total cholesterol concentrations was achieved by dose 4,5 mg/kg bw, which is also an effective dose in lowering total cholesterol concentrations approached normal value. ;A research was done in to determine the effect of simplicia Alphitobius sp. intake on plasma total cholesterol concentration in white male rat Sprague-Dawley (Rattus norvegicus L.). Twenty-four male rats were separated into six treatment groups consisting of normal control group that were given a standard diet, treatment control group fed a high fat diet , and four treatment groups that were given a high fat diet and simplicia Alphitobius sp. with a different dose (2,25 mg/kg bw, 4,5 mg/kg bw, 6,75 mg/kg bw and 9 mg/kg bw). Provision of test material was done every day for 14 consecutive days. The result of one-way Anava test (P < 0,05) indicates that there is a significant effect on total cholesterol concentration at all treatment doses, showed that provision of simplicia Alphitobius sp. lower total cholesterol potentially. The biggest decrease of total cholesterol concentrations was achieved by dose 4,5 mg/kg bw, which is also an effective dose in lowering total cholesterol concentrations approached normal value. , A research was done in to determine the effect of simplicia Alphitobius sp. intake on plasma total cholesterol concentration in white male rat Sprague-Dawley (Rattus norvegicus L.). Twenty-four male rats were separated into six treatment groups consisting of normal control group that were given a standard diet, treatment control group fed a high fat diet , and four treatment groups that were given a high fat diet and simplicia Alphitobius sp. with a different dose (2,25 mg/kg bw, 4,5 mg/kg bw, 6,75 mg/kg bw and 9 mg/kg bw). Provision of test material was done every day for 14 consecutive days. The result of one-way Anava test (P < 0,05) indicates that there is a significant effect on total cholesterol concentration at all treatment doses, showed that provision of simplicia Alphitobius sp. lower total cholesterol potentially. The biggest decrease of total cholesterol concentrations was achieved by dose 4,5 mg/kg bw, which is also an effective dose in lowering total cholesterol concentrations approached normal value. ]

Abstrak :
Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberian fortifikan NaFeEDTA dalam susu kedelai terhadap kadar zat besi plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL). Sebanyak 25 ekor tikus putih jantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi pakan dan minum standar; KK 2 yang diberi pakan minum standar dan susu kedelai tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi pakan minum standar dan susu kedelai dengan fortifikan NaFeEDTA berturut-turut dosis 1,35 mg Fe/ kgBB, 2,7 mg Fe/ kg BB, dan 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah perlakuan pada hari ke-21. Darah dipreparasi menggunakan destruksi basah lalu ditentukan kadar zat besinya dengan AAS (Atomic Absorption Spectrophotometer). Hasil uji ANAVA satu arah dan uji LSD (P < 0,05) menunjukkan perbedaan nyata pemberian fortifikan NaFeEDTA dalam susu kedelai terhadap kadar zat besi antar kelompok perlakuan. Peningkatan kadar zat besi tertinggi terjadi pada KP 3 di hari ke-21 yaitu 31,74% terhadap KK 1; dan 23,52% terhadap KK 2.

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The effect of NaFeEDTA fortificant addition to soymilk on plasma iron concentration of male Sprague-Dawley rats (Rattus norvegicus L.) had been studied. By using Complete Random Design (CRD), twenty five rats were divided into five groups. Normal control group (KK 1) which was administered with standard feeding and drinking only. Treatment control group (KK 2) which was administered with extra soymilk non fortificant, and three treatment groups which were administered with extra soymilk added with NaFeEDTA fortificant 1.35 mg Fe/kgbw (KP 1); 2.7 mg Fe/kgbw (KP 2); and 5.4 mg Fe/kgbw (KP 3). All of the five groups were treated for 21 days consecutively. The plasma iron concentration was measured by Atomic Absorption Spectrophotometer (AAS). One way ANOVA test and post hoc LSD test (P < 0.05) showed significant effect of NaFeEDTA fortificant addition to soymilk on plasma iron concentration in all treatment groups. The highest increase of plasma iron concentration was detected on KP 3 at t21 which is 31.74% to KK 1; and 23.52% to KK 2.

Abstrak :
Penelitian untuk mengetahui pengaruh fortifikan NaFeEDTA dalam tepung tahu terhadap kadar hemoglobin darah tikus jantan telah dilakukan. Metode penelitian berupa Rancangan Acak Lengkap, terdiri atas 25 ekor tikus putih yang dibagi menjadi lima kelompok perlakuan yaitu KK1 yang diberi minuman, pakan standar dan larutan CMC 0,5 ; KK2 yang diberi minuman, pakan standar, larutan CMC 0,5 dan tepung tahu 0,45 g tanpa fortifikan; serta KP 1, KP 2, KP 3 yang diberi minuman, pakan standar, larutan CMC 0,5 dan tepung tahu 0,45 g dengan fortifikan NaFeEDTA dosis 2,7 mg Fe/kgBB; 5,4 mg Fe/kgBB; dan 10,8 mg Fe/kgBB selama 14 hari. Pengukuran kadar hemoglobin darah dilakukan pada hari ke-0 dan 14 menggunakan Hematology Analyzer. Hasil uji Saphiro-Wilk dan Levene menunjukkan data terdistribusi normal dan homogen. Uji ANAVA satu arah menunjukkan hasil pengaruh nyata pemberian fortifikan NaFeEDTA terhadap kadar hemoglobin antar kelompok perlakuan. Hasil uji LSD menunjukkan terdapat perbedaan kadar hemoglobin nyata antara KK1 dan KK 2 dengan KP 1, KP 2 dan KP 3; serta KP 2 dengan KP 1 dan KP 3. Peningkatan tertinggi kadar hemoglobin darah terdapat pada KP 2 yaitu sebesar 15,15 dalam kelompok; 11, 76 terhadap KK 1; dan 10,95 terhadap KK 2.

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Research to find out the effect of fortificant NaFeEDTA in tofu flour on blood hemoglobin levels in male rats has been done. Twenty five rats were divided into five groups, consist of normal control group KK1 which was given standard feed, drinks and solution of CMC 0.5 treatment control group KK 2 which was given standard feed, drinks, solution of CMC 0,5 , and 0,45 g tofu flour without fortificant and three treatment groups KP 1, KP 2, KP 3 which were given standard feed, drinks, solution of CMC 0,5 , and 0,45 g tofu flour with NaFeEDTA 2.7 mg Fe kgBB 5.4 mg Fe kgBB and 10.8 mg Fe kgBB for 14 days. Measurement of blood hemoglobin levels done on day 0 and 14 using Hematology Analyzer. One way ANAVA test shows significant effect of NaFeEDTA on hemoglobin levels in all treatment groups. LSD test shows the blood hemoglobin level was significantly different between KK1 and KK 2 with KP 1, KP 2, and KP 3 as well as KP 2 with KP 1 and KP 3. The highest increase in blood hemoglobin levels was detected on KP 2 which is 15.15 within the group 11, 76 against KK 1 and 10.14 against KK 2.

Abstrak :
Latar Belakang : Monosodium glutamat (MSG) mengandung glutamat yang apabila terakumulasi akan mengakibatkan kerusakan berbagai sel dan organ, salah satunya adalah sel Leydig. Sel Leydig memiliki kemampuan regenerasi setelah mengalami kerusakan. Penelitian ini bertujuan mengetahui kemampuan regenerasi sel Leydig tikus dewasa yang rusak akibat pajanan MSG. Metode : Penelitian ini menggunakan 27 ekor tikus putih jantan strain Sprague Dawley usia 10-12 minggu yang dibagi menjadi 9 kelompok. Kelompok kontrol (K) diberikan aquadest 1,5 ml, kelompok PI diberikan MSG 4g/kgBB, dan kelompok PII diberikan MSG 6g/kgBB. Perlakuan diberikan secara oral selama 30 hari. Dari masing-masing perlakuan akan dibagi menjadi kelompok yang dimatikan 1 hari, 14 hari, dan 28 hari pasca perlakuan terakhir dihentikan. Testis kanan dan hipofisis dibuat sajian histologi menggunakan pewarnaan HE dan PAS. Parameter yang diamati adalah jumlah sel Leydig, sel berinti lonjong intersisial tubulus seminiferus, dan sel basofil adenohipofisis. Hasil : Peningkatan dosis MSG menyebabkan penurunan jumlah sel Leydig, serta peningkatan jumlah sel berinti lonjong intersisial tubulus seminiferus yang diduga merupakan sel progenitor Leydig. Pajanan MSG juga menyebabkan penurunan jumlah sel basofil adenohipofisis. Setelah pajanan MSG dihentikan selama 14 hari dan 28 hari, tejadi peningkatan jumlah sel Leydig, penurunan jumlah sel berinti lonjong, dan peningkatan jumlah sel basofil. Kesimpulan : Sel leydig memiliki kemampuan regenerasi yang berlangsung antara 14 hingga 28 hari setelah penghentian pajanan MSG. ......Background : Monosodium glutamate (MSG) contains glutamate which if accumulated will result in damage to various cells and organs, one of which in the Leydig cells. Leydig cells had the ability to regenerate after damage. This study aims to investigate the Leydig cells regeneration of adult male rats after cessation of MSG exposure. Methods: This study was performed on twenty-seven Sprague Dawley male rats (10-12 weeks old). They were divided into 9 groups. Control group (K) was given aquadest 1,5ml/day and two treated groups (PI and PII ) were given MSG 4g/kgBB and 6 g/kgBB. Treatment was given orally during 30 days. Each group was then divided into three groups that were sacrificed 1 day, 14 days and 28 days after the last treatment. Histological preparations of the right testes and pituitary was studied using HE and PAS staining, respectively. The number of Leydig and oval nucleated cells of the seminiferous tubules interstitial as well as basophil cells of adenohypophysis were observed. Result : Monosodium glutamate exposure caused a dose-dependent decrease in the number of Leydig cells and an increase in the number of oval nucleated cells. It was suggested that the oval nucleated cells were leydig progenitor cells. Monosodium glutamate exposure also caused a decrease in the number of basophil cells of adenohypophysis. After cessation of MSG for 14 and 28 day, there was an increase in the number of Leydig cells, a decrease in the number of oval nucleated cells and an increase the number of basophil cells. Conclusion : Leydig cells had the ability to regenerate and the regeneration took place between 14 and 28 days after cessation of MSG exposure.

Abstrak :
Penelitian ini bertujuan untuk mengetahui apakah 3,75%; 7,5%; dan 15% konsentrasi etilen glikol (EG) dan susu skim (SS) dalam vitrifikasi dapat mempengaruhi morfologi ovarium tikus (Rattus norvegicus L.) Sprague-Dawley fase proestrus. Ovarium yang digunakan dalam penelitian berasal dari tikus dengan usia 12 minggu dan diisolasi ketika fase proestrus kemudian vitrifikasi selama 48 jam. Ovarium dibagi menjadi sembilan kelompok dengan tiga pengulangan, yaitu KK 1, KK 2, KK 3, KKP 1, KKP 2, KKP 3, KP 1, KP 2, dan KP 3. KK 1, KK2, dan KK 3 adalah ovarium fase proestrus tanpa vitrifikasi. KKP 1, KKP 2, dan KKP 3 adalah ovarium fase proestrus yang divitrifikasi menggunakan EG dengan konsentrasi 3,75%; 7,5%; dan 15%. KP 1, KP 2, dan KP 3 adalah ovarium fase proestrus yang vitrifikasi menggunakan kombinasi EG dan SS dengan konsentrasi 3,75%; 7,5%; dan 15%. Hasil penelitian menunjukkan bahwa rata-rata jumlah folikel preantral dengan morfologi utuh pada KKP 1, KKP 2, KKP 3, KP 1, KP 2, dan KP 3 lebih rendah dibandingkan KK dan tidak berbeda nyata. Namun, penelitian ini menunjukkan bahwa vitrifikasi ovarium tikus dengan etilen glikol dan susu skim memiliki pengaruh terhadap morfologi folikel preantral tikus.

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The research aimed to find out whether 3,75%; 7,5%; and 15% concentration of ethylene glycol (EG) and skimmed milk (SM) in vitrification can influence the development of ovary of rat (Rattus norvegicus L.) strain Sprague-Dawley during the proestrus phase. The test ovary used were from rat with age 12 weeks and isolated when proestrus phase then vitrified for 48 hours. The test ovaries were divided into nine groups with three repetitions, namely KK 1, KK 2, KK 3, KKP 1, KKP 2, KKP 3, KP 1, KP 2, and KP 3. KK 1, KK2, and KK 3 are proestrus ovary without vitrification. KKP 1, KKP 2, and KKP 3 are proestrus ovary that vitrification on EG with concentrations of 3,75%; 7,5%; and 15%. KP 1, KP 2, and KP 3 are proestrus ovary that vitrification on EG and SM with concentrations of 3,75%; 7,5%; and 15%. The results showed that the average of the preantral follicle ovary in KKP 1, KKP 2, KKP 3, KP 1, KP 2, and KP 3 are lowest than KK and not were significantly different. However, this research showed that vitrification of rat ovary with ethylene glycol and skimmed milk may have effect to ovary morphology.