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"Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) merupakan coronavirus baru, pertama kali terdeteksi di Wuhan, Cina. Tingginya jumlah kasus dan cepatnya proses infeksi membutuhkan deteksi cepat dan akurat untuk menegakan diagnosis COVID-19. Studi bertujuan memperoleh sensitivitas dan spesifisitas uji serologi IgG SARS-CoV-2 Architect dibandingkan dan RT-PCR pada pasien COVID19. Studi ini menggunakan pendekatan potong lintang uji diagnostik, dengan menggunakan 128 pasien yang diperoleh di RSUP Persahabatan. Studi ini berlangsung sejak bulan April sampai Juli 2021 di RSUP Persahabatan. Sampel berupa swab nasofaring diambil dari pasien untuk diperiksa dengan uji RT PCR dan darah untuk uji serologi menggunakan Architect ® i2000SR Abbott. Hasil uji sensivitas Architect sebesar 27.37% (18.72%-37,48%) dan spesifisitas 63.64% (45,12% - 79,60%). NPV 23,33% dan PPV 68,42%. Kesimpulan: Secara keseluruhan uji serologi SARS-CoV-2 IgG Architech memiliki sensitivitas dan spesifisitas yang rendah dibanding dengan RTPCR. Architect dapat digunakan untuk screening, dapat digunakan pada fase akut, hasil negatif perlu dikonfirmasi dengan RT-PCR. Tidak ada hubungan antara Ct-value RTPCR dengan derajat keparahan COVID-19, dan terdapat hubungan antara titer Architect dengan derajat keparahan COVID-19. Tidak ada hubungan onset COVID-19 dengan hasil pemeriksaan RT-PCR dan Architect

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Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a new coronavirus, which was first detected in Wuhan, China. The high number of cases and the rapid process of infection require fast and accurate detection to establish the diagnosis of COVID-19. The study aimed to obtain the sensitivity and specificity of the SARS-CoV2 Architect IgG serological test compared to RT-PCR in COVID-19 patients. This study used a cross-sectional approach to diagnostic testing, using 128 patients obtained from Persahabatan Hospital. This study took place from April 2021 to July 2021 at the Persahabatan Hospital. Nasopharyngeal swab samples were taken for RT-PCR and blood was drawn for serological testing using the Architect® i2000SR from Abbott. The sensitivity of Architect test was 27.37% (18.72–37.48%), specificity was 63.64% (45.12–79.60%), whereas NPV was 23.33% and PPV was 68.42%. Conclusion: Architech's SARS-CoV-2 IgG serological test has low sensitivity and specificity compared to RT-PCR. Architect can be used for screening, can be used in the acute phase, negative results need to be confirmed by RT-PCR. There is no relationship between the Ct-value RT-PCR and the severity of COVID-19, and there is a relationship between Architect titers and the severity of COVID-19. There is no relationship between the onset of COVID-19 and the results of the RT-PCR and Architect examinations."

"Penelitian dengue secara in vitro banyak dilakukan untuk mengetahui mekanisme pasti patogenesis infeksi degue. Namun, sedikitnya informasi mengenai karakter pertumbuhan virus dengue pada galur sel model menjadi salah satu faktor pembatas. Karakterisasi pertumbuhan kinetik dilakukan untuk mengetahui dengan melihat karakter pertumbuhan kinetik virus dengue pada galur sel C6/36, Vero76, MDCK, 293, HepG2, dan A549. Parameter pertumbuhan virus dengue pada tiap sel diketahui dengan melihat kecepatan replikasi, ekspresi protein NS1, dan deteksi genom virus dengue. Hasil uji kinetik menunjukkan adanya perbedaan profil pertumbuhan tiap serotipe virus dengue pada tiap galur sel, dengan pertumbuhan relatif lebih tinggi pada sel A549 dibandingkan dengan galur sel lainnya. Hasil uji ELISA menunjukkan ekspresi protein NS1 pada sel A549 meningkat seiring peningkatan titer virus. Keberadaan RNA genom virus dengue pada sel A549 dikonfirmasi menggunakan RT-PCR. Dengan demikian, keseluruhan hasil penelitian ini menunjukkan virus dengue mampu tumbuh dengan baik pada galur sel A549, sehingga dapat digunakan sebagai galur sel mamalia alternatif untuk propagasi dan penelitian infeksi virus dengue lebih lanjut.

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In vitro dengue research has been routinely used to study the pathogenesis of dengue infection. The little information about the growth characteristics of dengue viruses in various cell lines model has become the limiting factor of dengue research. Dengue virus growth characterization was conducted to determine the growth kinetics of dengue viruses in several cell lines i.e. in C6/36, Vero76, MDCK, 293, HepG2, and A549 cell lines. Growth characteristics of dengue virus in each cell were measured by looking at the rate of replication, NS1 protein expression, and detection of dengue virus genome. The replication kinetic assay indicated the difference growth characteristics of each serotype of dengue virus in each cell line, with the relatively higher growth was observed in A549 cell line compared to other cell lines. ELISA result showed an increased expression of NS1 protein in A549 cell in parallel with increasing viral titer. The presence of dengue virus RNA genome in A549 cells was confirmed using RTPCR assay. This study observed the ability of dengue viruses to grow well in A549 cell line and this cell line could be used as an alternative mammalian cell line for propagation and further study of dengue virus infection."