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Abstrak :
Mikroalga Nannochloropsis sp. memiliki kandungan biomassa bervariasi, salah satunya protein. Hidrolisis protein menghasilkan peptida dan asam amino sehingga meningkatkan bioaktivitas dari protein. Penelitian ini bertujuan untuk mendapatkan protein hidrolisat dari mikroalga Nannochloropsis sp. serta mengetahui keamanan dan efektivitasnya sebagai produk kosmetik anti-aging. Protein hidrolisat mikroalga Nannochloropsis sp. (PHMN) diperoleh dari proses hidrolisis menggunakan enzim alkalase. PHMN dievaluasi perolehan rendemen, derajat hidrolisis, kandungan proksimat, asam amino serta aktivitas anti-elastasenya. PHMN diformulasikan menjadi sediaan emulgel, kemudian dievaluasi sifat fisikokimia dan stabilitasnya. Uji keamanan produk kosmetik PHMN dilakukan dan efektivitas produk kosmetik PHMN sebagai anti-aging dievaluasi dengan mengukur serat kolagen, elastisitas, dan kelembaban kulit relawan. Protein hidrolisat yang diperoleh memiliki nilai rendemen sebesar 25,77%±3,16% (b/b), derajat hidrolisis sebesar 36,73%, dan mengandung asam amino yang didominasi oleh asam glutamat, asam aspartat, dan leusin. Nilai konsentrasi penghambatan setengah maksimal (IC50) PHMN sebagai anti-elastase yaitu 244,43 mg/mL. Produk kosmetik PHMN menunjukkan stabilitas yang baik yaitu homogen dan kadar yang stabil setelah 24 minggu. Penggunaan produk kosmetik PHMN tidak menyebabkan iritasi dan meningkatkan serat kolagen, elastisitas, dan kelembaban kulit setelah 28 hari. Dari penelitian ini disimpulkan bahwa diperoleh protein hidrolisat dari mikroalga Nannochloropsis sp. serta produk kosmetik PHMN memiliki karakteristik yang baik, aman, dan efektif sebagai anti-aging ......Microalgae Nannochloropsis sp. contain various biomass composition including protein. Protein hydrolysis generate peptide and amino acids so that protein bioactivity improves. The purpose of study was to obtain protein hydrolysate from Nannochloropsis sp. microalgae, also assess its safety and efficacy as anti-aging cosmetic product. Nannochloropsis sp. protein hydrolysate (NPH) was obtained from microalgae through enzymatic hydrolysis using alcalase enzyme and characterized for yield, degree of hydrolysis (DH), proximate content, amino acids composition, and anti-elastase activity. NPH was formulated as emulgel, then evaluated for physical characteristics and stability. NPH cosmetic product was evaluated for safety and efficacy as anti-aging by measuring collagen fibers, elasticity, and moisture in volunteers. NPH was obtained with yield of 25.77±3.16% (w/w), DH value of 36.73%, and amino acids dominated by glutamic acid, aspartic acid, and leucin. Half-maximum inhibitory concentration (IC50) value as anti-elastase was 244.43 µg/mL. NPH cosmetic product showed good stability which homogenous and had stable protein content after 24 weeks storage. NPH cosmetic product usage did not cause skin irritation and increased collagen fiber, elasticity and moisture after 28 days. In conclusion, protein hydrolysate was obtained from Nannochloropsis sp. microalgae and NPH cosmetic product had good characteristic, safe, and effective as anti-aging.

Abstrak :
Mikroalga memiliki potensi untuk dijadikan sumber bahan baku biodiesel. Pada penelitian ini, lipid mikroalga Nannochloropsis sp. akan disintesis menjadi biodiesel melalui reaksi transesterifikasi menggunakan katalis heterogen CuO/Zeolit. Penelitian ini dilakukan untuk menentukan kondisi reaksi transesterifikasi yang optimum, meliputi suhu dan waktu, dalam menghasilkan yield biodiesel tertinggi, serta mendapatkan komposisi dan kandungan FAME dari biodiesel yang dihasilkan. Dari hasil yang diperoleh, yield biodiesel tertinggi dicapai pada suhu 60oC selama 3 jam dengan yield sebesar 53,1% b/b lipid dan 10,6% b/b biomassa. Kandungan FAME yang terdapat pada biodiesel mikroalga Nannochloropsis sp. didominasi oleh ester dari asam lemak C16:0 (22,7%) dan C18:1 (63,8%).

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Microalgae have a potential as a feedstock of biodiesel. In this study, lipid from microalgae Nannochloropsis sp. was synthesized into biodiesel through transesterification reaction using heterogeneous catalyst CuO/Zeolite. The objectives are to obtain the optimum reaction conditions, including temperature and time, that produce highest yield of biodiesel, and to obtain the composition and content of FAME from microalgal biodiesel. From the results, highest yield achieved at 60°C for 3 hours with a yield of 53,1% w/w of lipid and 10,6% w/w of biomass. The content of FAME in biodiesel dominated by esters from fatty acid C16:0 (22,7%) and C18:1 (63,8%).

Abstrak :
Mikroalga adalah salah satu sumber biofuel yang menjanjikan karena memiliki kapasitas produksi lipid yang dapat dimanfaatkan sebagai bahan baku produksi biodiesel, terutama Nannochloropsis sp. dan Chlorella vulgaris. Lipid yang dihasilkan dapat diolah menjadi biodiesel melalui reaksi transesterifikasi menggunakan katalis homogen atau heterogen. Katalis heterogen memiliki kelebihan dibandingkan katalis homogen karena bentuknya yang padat memudahkan proses pemisahan katalis dari campuran produk. Dalam penelitian ini, digunakan katalis heterogen basa NaOH/zeolit dengan variasi loading Na dalam zeolit untuk melihat pengaruhnya terhadap yield biodiesel yang dihasilkan dari Nannochloropsis sp. dan Chlorella vulgaris. Hasil terbaik didapatkan dengan konsentrasi loading Na sebesar 20,5 . Yield biodiesel terhadap lipid yang didapatkan adalah sebesar 83,5 dari Nannochloropsis sp. dan 98 dari Chlorella vulgaris. Biodiesel yang dihasilkan memiliki kandungan asam lemak jenuh metil ester sebanyak 47,14 dari Nannochloropsis sp. dan 56,41 dari Chlorella vulgaris.

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Microalgae are promising sources of biofuel due to its production capacity of lipid that can be utilized as raw material for biodiesel production, especially Nannochloropsis sp. and Chlorella vulgaris. The lipid produced can be converted into biodiesel through transesterification reaction using homogenous or heterogeneous catalysts. Heterogeneous catalysts are more advantageous than homogeneous catalysts due to its solid form that eases the separation of catalysts from the products. In this research, NaOH zeolite heterogeneous catalyst is utilized with varying Na loadings in the zeolite to observe its effect towards the yield of biodiesel produced from Nannochloropsis sp. and Chlorella vulgaris. The best result was obtained with Na loading concentration of 20.5 . The biodiesel yields obtained from the lipids are 83.5 from Nannochloropsis sp. and 98 from Chlorella vulgaris. The biodiesels contain 47.15 of saturated fatty acid methyl esters from Nannochloropsis sp. and 56.41 from Chlorella vulgaris.

Abstrak :
Pemanfaatan biodiesel sawit diselidiki memiliki masalah dengan sifat aliran dingin terutama di daerah dataran tinggi yaitu titik kabut tinggi karena tingginya kadar asam lemak jenuh. Di sisi lain, minyak mikroalga memiliki titik kabut yang rendah karena tingginya tingkat asam lemak tak jenuh. Sementara itu, densitas dan kadar air biodiesel yang terlalu tinggi akan mempengaruhi kinerja mesin. Oleh karena itu, kombinasi sifat tak jenuh tunggal dan jenuh, maupun perbedaan densitas antara minyak kelapa sawit dan minyak mikroalga membuatnya lebih disukai sebagai campuran bahan baku untuk meningkatkan kualitas Biodiesel. Penelitian ini memformulasikan pencampuran minyak mikroalga Nannochloropsis sp (MO1) dan/atau minyak mikroalga Chlorella vulgaris (MO2) terhadap minyak sawit (PO). Skema pencampuran minyak dilakukan dengan 4 variasi yaitu perbandingan massa 0:30 (MO:PO); 1:30 (MO1:PO); 1:30 (MO2:PO); 1:1:30 (MO1:MO2:PO).Transesterifikasi terjadi pada 65°C dengan penambahan katalis KOH dengan perbandingan minyak : metanol (1 : 15) : @250 mL selama 1,5 jam. FAME dianalisis berdasarkan SNI 7182:2015 dengan tiga parameter utama diantaranya kadar air (ASTM D6304), densitas (ASTM D1298), dan titik kabut (ASTM D2500). Berdasarkan penelitian yang dilakukan dari semua variasi menunjukkan bahwa hasil terbaik yang direkomendasikan adalah komposisi campuran minyak mikroalga Chlorella vulgaris terhadap minyak sawit dengan parameter densitas dan titik kabut masing – masing sebesar 859,3 kg/m3 dan 11,7°C (sesuai SNI), meskipun parameter kadar air masih tinggi sebesar 973,4 mg/kg (tidak sesuai SNI). ......Utilization of palm biodiesel is investigated having problems with the cold flow properties particularly in the high-altitude areas. A common problem of biodiesel is the high cloud point due to high levels of saturated fatty acids. On the other hand, microalgae oil has a low cloud point due to high levels of unsaturated fatty acids. Meanwhile, the density and moisture content of biodiesel that is too high will also affect engine performance. Therefore, the combination of monounsaturated and saturated properties, as well as differences in density between palm oil and microalgae oil make it preferred as a mixture of raw materials to improve the quality of Biodiesel. This research formulated the mixing of Nannochloropsis sp (MO1) microalgae oil and/or Chlorella vulgaris (MO2) microalgae oil to palm oil (PO). The oil mixing scheme is carried out with 4 variations namely mass ratio 0:30 (MO: PO); 1:30 (MO1: PO); 1:30 (MO2: PO); 1: 1: 30 (MO1: MO2: PO). The transesterification occurred at 65°C with the addition of KOH catalyst with oil : methanol (1 : 15) : @250 mL during 1,5 hours. The FAMEs were analysis according to SNI 7182:2015 with three main parameters including water content (ASTM D 6304), density (ASTM D1298), and cloud point (ASTM D 2500). Based on research conducted from all variations shows that the best results recommended are the composition of a mixture of Chlorella vulgaris microalgae oil to palm oil with the density and fog point parameters respectively 859.3 kg/m3 and 11.7 °C (according SNI), although the water content parameter is still high at 973.4 mg/kg (not according SNI).

Abstrak :
Algae are a promising source of biofuel but claims about their lipid content can be ambiguous because extraction methods vary and lipid quantitation often does not distinguish between particular lipid classes. One of algae types that meet this condition is Nannochloropsis sp.

Abstrak :
ABSTRAK
Reaksi transesterifikasi langsung membutuhkan optimasi kondisi reaksi disebabkan proses ekstraksi lipid dan reaksi transesterifikasi terjadi di tempat dan waktu yang sama. Pemanfaatan co-solvent dianggap sebagai salah satu cara untuk meningkatkan yield dalam reaksi transesterifikasi langsung. Pada penelitian ini dilakukan transesterifikasi langsung pada mikroalga chlorophyta yaitu Nannochloropsis sp dan Chlorella vulgaris. Desain variasi yang dilakukan mencakup rasio volum metanol : n-heksana, rasio molar lipid : metanol, dan waktu reaksi. Yield biodiesel diukur secara gravimetri. Dari variasi ini, kondisi rasio volum metanol : n-heksana 1:1, rasio molar lipid : metanol 1:400, dan waktu reaksi 4 jam mampu meningkatkan yield biodiesel mencapai 94,93% untuk C.vulgaris dan 90,9% untuk Nannochloropsis sp. Biodiesel yang diperoleh dianalisis kandungan FAMEnya dengan Kromatografi Gas dan Spektroskopi Massa (GCMS). Kandungan asam lemak jenuh lebih dominan pada biodiesel dari Nannochloropsis sp. mencapai 52,72%.

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ABSTRACT
Direct transesterification reaction requires optimization of reaction conditions due to lipid extraction and transesterification reactions that occur at the same place and time. Utilization of co-solvent is considered as one way to increase biodiesel yield in the direct transesterification reaction. In this research, the direct transesterification was done on Chlorophyta microalgae, those are Chlorella vulgaris and Nannochloropsis sp. Design variations are including the volume ratio of methanol: n-hexane, the molar ratio of lipid: methanol, and reaction time. Biodiesel yield was measured gravimetrically. From these variations, the volume ratio of methanol n-hexane 1:1, molar ratio of lipid:methanol 1:400, and reaction time 4 hours can increase biodiesel yield until 94.93% for C.vulgaris and 90.9% for Nannochloropsis sp. FAME contents in biodiesel were analyzed by Gas Chromatography and Mass Spectroscopy (GCMS). Saturated fatty acid content is more dominant on biodiesel from Nannochloropsis sp. reached 52.72%.

Abstrak :
Medium kultur mengambil salah satu peranan penting dalam pertumbuhan mikroalga berkaitan dengan sumber nutrisi. Penggunaan limbah cair domestik maupun industri telah menjadi perhatian para peneliti sebagai medium kultivasi mikroalga. Pada penelitian ini, Nannochloropsis sp. dikultivasi dalam fotobioreaktor pelat datar menggunakan limbah cair tapioka dengan konsentrasi 10%, 20%, 30% dan 40%. Hasil penelitian menunjukkan medium limbah cair tapioka 20% menghasilkan pertumbuhan Nannnochloropsis sp. yang optimal dan memberikan produktivitas lipid tertinggi sebesar 13,00%. Pasca kultivasi Nannochloropsis sp., parameter BOD, COD, amonia dan fosfat dalam limbah cair tapioka juga menurun cukup signifikan. Studi ini dapat digunakan sebagai dasar dalam perbesaran skala industri biodiesel mikroalga berbasis limbah. ......Cultivation medium takes one important role on microalgae’s growth related to nutrient source. The utilization of domestic and industrial wastewater has attracted researchers attention as a cultivation medium of microalgae. In this study, Nannochloropsis sp. was cultivated in microalgal flat plate photobioreactor using tapioca wastewater diversing in concentration 10%, 20%, 30% and 40%. The results indicated that the optimum of Nannochloropsis sp. growth found in cultivation by 20% tapioca waste medium and gives highest lipid yield,13,00%. After the cultivation of Nannochloropsis sp., BOD, COD, ammonia and phosphates in tapioca wastewater also decreased significantly. This study can be used as a basis in the enlarged scale wastewater-based microalgae biodiesel industry.

Abstrak :
[Salah satu sumber alternatif yang dapat dijadikan sebagai bahan bakar minyak adalah mikroalga Nannochloropsis sp. yang ternyata memiliki potensi sebagai bahan pangan minyak nabati. Karakterisasi mikroalga untuk memantau pertumbuhan sel dan mengetahui jumlah lipid yang terkandung di dalamnya, secara berturut-turut menggunakan kapasitor plat sejajar dan ultrasonic cleaner. Pengukuran jumlah sel alga menggunakan kapasitor plat sejajar sebagai alternatif alat penghitung sel ini kemudian dibandingkan dengan spektrofotometer Uv-Vis dan Counting Chamber untuk mendapatkan penghitungan yang valid. Sedangkan ekstraksi mikroalga menggunakan alat pembersih ultrasonik sederhana dengan frekuensi 48kHz. Pengukuran dengan menggunakan kapasitor dianggap valid karena kurva kenaikannya sebanding dengan jumlah selnya. Sedangkan hasil ekstraksi lipid dari mikroalga Nannochloropsis sp. mencapai nilai optimum pada menit ke-60 dengan menggunakan pelarut metanol sebesar 7.50% dari massa keringnya. ......One source of alternative that can be used as fuel oil is microalgae Nannochloropsis sp which turns have the potential of food as a vegetable oil. Characterization of microalgae to monitoring the growth of cells and knowing the number of lipids contained in it, respectively the use of a capacitor plate parallel and ultrasonic cleaner.The measurement of the number of cells using algae capacitor plate parallel as an alternative instrument coalition cells were then compared with the spectrophotometer uv-vis and counting chamber to get a valid calculation. While the extraction of microalgae used a simple cleaning ultrasonic 48khz with a frequency. Measurements using a capacitor considered valid because a curve inflation figure comparable to cell number. While the results of the extraction of lipid mikroalga Nannochloropsis sp. achieve optimum on the 60th minute by using methanol solvent by 7.50 % of a mass of the drying up.;One source of alternative that can be used as fuel oil is microalgae Nannochloropsis sp which turns have the potential of food as a vegetable oil. Characterization of microalgae to monitoring the growth of cells and knowing the number of lipids contained in it, respectively the use of a capacitor plate parallel and ultrasonic cleaner.The measurement of the number of cells using algae capacitor plate parallel as an alternative instrument coalition cells were then compared with the spectrophotometer uv-vis and counting chamber to get a valid calculation .While the extraction of microalgae used a simple cleaning ultrasonic 48khz with a frequency .Measurements using a capacitor considered valid because a curve inflation figure comparable to cell number. While the results of the extraction of lipid mikroalga Nannochloropsis sp. achieve optimum on the 60th minute by using methanol solvent by 7.50 % of a mass of the drying up, One source of alternative that can be used as fuel oil is microalgae Nannochloropsis sp which turns have the potential of food as a vegetable oil. Characterization of microalgae to monitoring the growth of cells and knowing the number of lipids contained in it, respectively the use of a capacitor plate parallel and ultrasonic cleaner.The measurement of the number of cells using algae capacitor plate parallel as an alternative instrument coalition cells were then compared with the spectrophotometer uv-vis and counting chamber to get a valid calculation .While the extraction of microalgae used a simple cleaning ultrasonic 48khz with a frequency .Measurements using a capacitor considered valid because a curve inflation figure comparable to cell number. While the results of the extraction of lipid mikroalga Nannochloropsis sp. achieve optimum on the 60th minute by using methanol solvent by 7.50 % of a mass of the drying up]

Abstrak :
ABSTRAK
Minyak kelapa sawit merupakan bahan baku unggul pembuatan biodiesel di Indonesia. Meskipun Indonesia memiliki iklim tropis, Pemanfaatan biodiesel sawit diselidiki memiliki masalah dengan sifat aliran dingin terutama di daerah-daerah dataran tinggi. Masalah umum lainnya dari Biodiesel adalah kemudahan degradasi selama penyimpanan jangka panjang. Minyak kelapa sawit memiliki stabilitas oksidasi yang lebih baik, tetapi titik awannya tinggi karena tingginya kadar asam lemak jenuh. Di sisi lain, minyak mikroalga memiliki titik kabut dan titik tuang yang rendah, tetapi lebih mudah teroksidasi karena tingginya tingkat FAME tak jenuh. Oleh karena itu, kombinasi sifat tak jenuh tunggal dan jenuh antara Minyak Kelapa Sawit dan minyak Mikroalga membuatnya lebih disukai sebagai campuran bahan baku untuk meningkatkan kualitas Biodiesel. Model senyawa alga metil diformulasikan berdasarkan komposisi asam lemak Nannochloropsis sp. dari literatur. Dalam penelitian ini, skema pencampuran minyak dilakukan dengan variasi 5%, 10%, 20%, 30% penambahan Microalgae Oil ke Palm Oil. Transesterifikasi terjadi pada 60-700C dengan penambahan katalis basa 0,6%-wt dan metanol 40% -v/v selama 1-1,5 jam. FAME dianalisis berdasarkan SNI 7182:2015 dengan empat parameter utama diantaranya angka asam total (ASTM D 6644), titik kabut dan titik tuang (ASTM D 2500, dan ASTM D97), dan stabilitas oksidasi (EN 14112). Tujuan dari penelitian ini ialah menentukan rasio campuran yang optimal antara minyak kelapa sawit dan minyak mikroalga untuk produksi biodiesel. Berdasarkan percobaan, rasio campuran biodiesel yang optimal ditemukan pada 5% dengan cloud dan titik tuang masing-masing adalah 15.30C dan 120C. Stabilitas oksidasi dan angka asam 5% yang diperoleh adalah 10,58 jam, dan 0,175 mg KOH /g. Oleh karena itu, campuran biodiesel mengkonfirmasi bahwa asam lemak tak jenuh dari minyak mikroalga dapat meningkatkan sifat aliran dingin dari bahan bakar biodiesel kelapa sawit.

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ABSTRACT
Palm oil is reported as the superior feedstock of biodiesel producing in Indonesia. Although Indonesia has tropical climate, Utilization of palm biodiesel is investigated having problems with the cold flow properties particularly in the high-altitude areas. The other common issue of Biodiesel is the ease of degradation during long-term storage. Palm oil has better oxidation stability, but high cloud point due to the high levels of saturated fatty acids. On the other hand, microalgae oil has low cloud and pour point, but more easily oxidized due to the high levels of unsaturated FAME. Therefore, the combination of monounsaturated and saturated properties between Palm Oil and Microalgae oil makes it preferable as raw materials blending to upgrade the quality of Biodiesel. The model algal methyl compounds were formulated based on fatty acid compositions of Nannochloropsis sp. from the literature. In this research, the oil blending scheme was done by variations 5%,10%,20%,30% of addition Microalgae Oil to Palm Oil. The transesterification occurred at 60-700C with the addition of base catalyst 0.6%- wt and methanol 40%-v/v during 1-1.5 hours. The FAMEs were analysis according to SNI 7182:2015 with four main parameters including total acid number (ASTM D 6644), cloud point and pour point (ASTM D 2500, and ASTM D97 respectively), and oxidation stability (EN 14112). The purpose of this research was to determine the optimum blending ratio between palm oil and microalgae oil for biodiesel production. Based on the experiment, the optimum blending ratio of biodiesel was found on 5% with the cloud and pour point are 15.30C and 120C respectively. The oxidation stability and total acid number of 5% blends obtained were 10.58 hours, and 0.175 mg KOH/g biodiesel respectively. Hence, the biodiesel blends confirm that the unsaturated fatty acids of microalgae oil can enhance the cold flow property of palm biodiesel fuels.

Abstrak :
Berbagai jalur produksi biohidrokarbon telah diteliti untuk mengembangkan potensi sumber energi terbarukan. Salah satu bentuk pengembangan ini dilakukan melalui reaksi konversi asam lemak dengan bantuan biokatalis fotodekarboksilase asam lemak dari mikroalga Chlorella variabilis (CvFAP). Meski potensinya besar, kekerabatan genetik spesies serta metode ekstraksi yang tepat untuk biomassa menjadi tantangan yang cukup signifikan. Potensi isolat mikroalga lokal Nannochloropsis sp. dalam menghasilkan biokatalis serupa yang dapat membantu reaksi sintesis hidrokarbon dari asam lemak selanjutnya diteliti. Mikroalga dikultur dan dipanen pada hari ke-7 untuk kemudian diekstraksi kandungan protein targetnya melalui pemisahan tiga fasa dengan bantuan sonikasi. Pengaruh pengeringan beku dilihat terhadap biomasssa yang diekstraksi (0,17 gr protein/L) dan memberikan nilai 54,5% lebih banyak daripada biomassa kondisi segar (0,11 gr protein/L); keduanya terkonsentrasi pada fasa tengah sistem ekstraksi pemisahan tiga fasa dengan bantuan sonikasi. Analisis SDS PAGE memberikan profil berat molekul serupa terhadap protein target (CvFAP) dengan pita-pita pemisahan senilai 60 kDa; 63 kDa; 64 kDa; dan 65 kDa. Hasil uji aktivitas enzim yang dilakukan pada substrat asam palmitat menunjukkan pembentukan hidrokarbon dari analisis GCMS dengan protein fresh culture (kelimpahan 5,06% sampel) maupun dengan protein freeze dry (kelimpahan 24,55% sampel). Adapun alkana yang terbentuk terbagi menjadi dua yakni alkana aromatik dan alkana bercabang (golongan rantai pendek) serta alkana rantai panjang dari rentang C20 hingga C30. Pentadekana (C15) tidak dihasilkan dalam reaksi konversi tersebut. ......Various biohydrocarbon production lines have been investigated to develop potential renewable energy sources. One form of this development is carried out through a fatty acid conversion reaction with the help of a fatty acid photodecarboxylase biocatalyst from the microalgae Chlorella variabilis (CvFAP). Despite the great potential, the genetic kinship of species and the appropriate extraction method for biomass pose a significant challenge. This study then researched the potential of local microalgae isolates Nannochloropsis sp. in producing similar biocatalysts that can assist in the synthesis of hydrocarbons from fatty acids. Microalgae were cultured and harvested on the 7th day and then the target protein content was extracted through ultrasound-assisted three phase partitioning (UATPP). The effect of freeze drying was seen on the extracted biomass (0.17 g protein/L) and gave a value of 54.5% more than the fresh biomass (0.11 g protein/L); both are concentrated in the middle phase of the UATPP system. SDS PAGE analysis provided a similar molecular weight profile of the target protein with 60 kDa main target bands of separation; 63 kDa; 64 kDa; and 65 kDa. The results of enzyme activity tests carried out with palmitic acid as a substrate showed the formation of hydrocarbons from GC-MS analysis with fresh culture protein (5.06% sample abundance) and freeze dried protein (24.55% abundance sample). The alkanes formed are divided into two, namely aromatic alkanes and branched alkanes (short chain alkanes group) and long chain alkanes (higher alkanes group) from the C20 to C30 range. Pentadecane (C15) was not formed during the conversion reaction.