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"Background: The prevalence of Helicobacter pylori (H. pylori) infection in the world is quite high, especially in developing countries. Usually the patient shows no specific symptoms and chronic gastritis therefore becomes chronically infected The complication of the injéction is the development of peptic ulcer; which is a predisposing factor for gastric carcinoma. Early diagnosis is an important step to avoid these complications by providing immediate accurate therapy. Methods: In this study the CLO, MIU (Motility Indole Urease) tests and culture were conducted on 131 biopsy samples of the stomach antrum mucous tissue taken from chronic dyspepsia patients from several hospitals in Jakarta. In the CLO test, biopsy tissue was put in a small well agar to be incubated at room temperature. In the MIU test the biopsy tissue sample was submerged in the small MlU tube agar with a depth of approximately 2/3 rds from the surface, and then incubated at room temperature. Another piece of biopsy tissue was cultured micro-aerophylicalty The CLO and MlU tests are considered positive if the color changes from yellow to red and are considered negative if there is no color change within 24 hours. Results: Compared to culture, the CLO test demonstrated 38% sensitivity; 96% specificity, 94% positive predictive value and 52% negative predictive value, whereas the results of the MIU test against culture method showed 76% sensitivity 89% specificity 88% positive predictive value, and 78% negative predictive value. Conclusion: The MIU test that showed high sensitivity and specyficity and thus could be further developed as an alternative diagnostic method for H. pylori infection."

"Latar Belakang: Diagnosis definitif Helicobacter pylori H.pylori hingga kini masih merupakan masalah. Biakan untuk isolasi dan identifikasi bakteri ini sulit. Uji cepat urease direkomendasikan sebagai uji diagnostik lini pertama pasien dispepsia. Tujuan: Mengembangkan komposisi medium biakan dan deteksi cepat H.pylori pada spesimen biopsi lambung pasien dispepsia. Metode: Desain penelitian merupakan studi potong lintang dan eksperimental laboratorium. Sampel diambil dengan cara consecutive sampling sebanyak 68 spesimen biopsi lambung 34 antrum, 34 korpus, masing-masing untuk biakan dan uji MIU. Sebagai pembanding digunakan histopatologi dan PCR. Mula-mula dilakukan optimasi medium biakan dan MIU konsentrasi merah fenol, pH, urea dan suhu inkubasi. Selanjutnya kondisi optimal yang diperoleh diaplikasikan pada spesimen biopsi pasien dispepsia. Hasil: Medium biakan agar darah Columbia ditambah vankomisin 5 mg / 500 mL dan darah domba 7 belum optimal, namun dapat digunakan untuk isolasi dan identifikasi. Hasil MIU modifikasi sebagai berikut: konsentrasi merah fenol 0,001 ; urea 4 ; pH medium 7; Suhu inkubasi optimal 35-370 C. Proporsi positif hasil uji MIU sebesar 35,29 12/34, biakan 32,35 11/34, PCR 32,35 11/34 dan histopatologi 20,59 7/34. Kesimpulan: Pemeriksaan MIU meningkatkan positivitas hasil pemeriksaan sebesar 14,7 bila dibandingkan dengan histopatologi.

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Background: Until now, definitive diagnostic of H.pylori is still a problem. Culture for isolation and identification of this pathogen is difficult. Rapid urease test is recommended as a first line diagnostic test.

Aim: To obtain optimal composition for culture medium and Motility Indol Urease MIU test for the detection of H. pylori in dyspeptic patient biopsy specimens.

Method: A cross sectional and experimental laboratory study was performed. Sixty eight gastric biopsy samples 34 antrum, 34 corpus were collected by consecutive sampling method for culture and MIU test. Histopathology and PCR were conducted for comparison. Initially, we performed the optimation of culture medium and MIU test phenol red and urea concentration, pH, and temperature. The optimal condition obtained was then applied to the specimens.

Result: Columbia agar supplemented with vancomycin 5 mg 500 mL and 7 sheep blood was unable to create an optimal condition, but it can be used for isolation and identification. Modified MIU was performed by this following condition phenol red 0,001 urea 4 pH 7 incubation temperature 35 37oC. Positive proportion of MIU was 35.29 12 34, culture 32.35 11 34, PCR 32,35 11 34 and histopathology 20.59 7 34.

Conclusion: MIU test was able to improve the positivity rate by 14,7 compared to histopathology."

"Omotenashi dalam masyarakat Jepang merupakan suatu bentuk kebudayaan yang umum ditemukan, terutama pada bisnis ryokan dan restoran. Meskipun kerap disebut sama dengan hospitality, namun omotenashi dapat dipahami sebagai konsep yang berbeda karena kental dengan unsur sejarah dan budaya masyarakat Jepang. Tulisan ini bertujuan untuk melihat penerapan omotenashi yang merupakan budaya Jepang pada restoran otentik Jepang yang berada di luar Jepang yaitu Miu Authentic Japanese Dining Indonesia. Metode yang akan digunakan dalam penelitian ini adalah studi deskriptif dan kualitatif dengan cara observasi, wawancara serta membaca pustaka yang relevan dengan penelitian, lalu dianalisis dengan konsep omotenashi dari Al-alsheikh (2014) dan didukung oleh konsep omotenashi oleh Ichijou (2015). Analisis yang dilakukan menghasilkan temuan bahwa 1) pada Miu Authentic Japanese Dining dapat ditemukan implementasi omotenashi yang menjadi sebuah bukti bahwa omotenashi dapat diterapkan di negara selain Jepang; 2) penerapan omotenashi di Miu Authentic Japanese Dining terdapat hambatan dari segi sumber daya manusia yang sebagian belum sadar akan pentingnya omotenashi dan perbedayaan budaya antara Jepang dan Indonesia. Studi kasus Miu Authentic Japanese Dining dapat menjadi pertimbangan untuk membuktikan kebudayaan Jepang yaitu omotenashi dapat diaplikasikan di luar negara asalnya dalam wujud bisnis restoran di Indonesia.

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Omotenashi is a form of culture that is commonly found in a Japanese Society, especially in ryokan and restaurant businesses. Even though it is often referred to as hospitality, omotenashi can be understood as a different concept because it is firmly rooted with historical and cultural elements of Japanese society. This paper aims to look at the application of omotenashi which is Japanese culture in authentic Japanese restaurants outside of Japan, namely Miu Authentic Japanese Dining in Indonesia. The method used in this study is a descriptive and qualitative study by means of observation, interviews and reading literature relevant to the research, then analyzed with the concept of omotenashi from Al-alsheikh (2014) and supported by the concept of omotenashi by Ichijou (2015). The analysis carried out resulted in the findings that 1) in Miu Authentic Japanese Dining one can find the implementation of omotenashi which is proof that omotenashi can be applied in countries other than Japan; 2) the application of omotenashi in Miu Authentic Japanese Dining has obstacles in terms of human resources, some of whom are not aware of the importance of omotenashi and the cultural differences between Japan and Indonesia. The case study of Miu Authentic Japanese Dining can be considered to prove that Japanese culture, namely omotenashi, can be applied outside its home country in the form of a restaurant business in Indonesia."