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Abstrak :
Kanker serviks menjadi kanker penyebab kematian tertinggi ketiga di Indonesia setelah kanker paru-paru dan payudara. Metode pencegahan seperti vaksin HPV dan metode pengobatan seperti pembedahan, kemoterapi, dan radioterapi telah dikembangkan. Akan tetapi, masih dibutuhkan pengembangan pengobatan alternatif, salah satunya adalah dari tumbuhan herbal dengan senyawa aktif citronellol. Penelitian-penelitian terdahulu membuktikan bahwa citronellol dapat menurunkan viabilitas dengan menginduksi apoptosis pada beberapa sel kanker, seperti sel kanker paru-paru, payudara, dan darah. Tujuan penelitian ini adalah untuk mengetahui pengaruh citronellol terhadap viabilitas sel HeLa menggunakan mikroskop fluoresens. Variasi konsentrasi 10, 25, 50, dan 100 µg/mL citronellol dengan waktu inkubasi 48 jam diujikan pada sel HeLa. Uji viabilitas dilakukan dengan uji WST-1 dan deteksi apoptosis dilakukan dengan pewarnaan Annexin V-FITC/PI menggunakan mikroskop fluoresens. Hasil uji dengan ANAVA satu arah menunjukkan tidak terdapat pengaruh signifikan dari citronellol terhadap viabilitas sel HeLa. Konsentrasi 10 µg/mL cenderung menurunkan viabilitas sel HeLa dibandingkan kontrol. Pengamatan dengan mikroskop fluoresens menunjukkan terdeteksinya sel apoptosis yang lebih tinggi dibandingkan dengan sel nekrosis dan perubahan morfologi pada sel late apoptosis. Penelitian lebih lanjut diperlukan untuk mengetahui lebih jauh pengaruh citronellol terhadap sel HeLa. ......Cervical cancer is the third leading cause of cancer death in Indonesia following lung cancer and breast cancer.  Prevention methods such as the HPV vaccine and treatment methods such as surgery, radiotherapy, and chemotherapy have been developed. However, the development of alternative medicine is still needed, one of which is from herbal plants with the active compound of citronellol. Previous studies have shown that citronellol can reduce viability by inducing apoptosis in some cancer cells, such as lung, breast, and blood cancer cells. This study aims to determine the effects of citronellol on HeLa cells viability and to detect apoptosis by Annexin V- FITC/PI staining using a fluorescence microscope. Concentration variations of 10, 25, 50, and 100 µg/mL citronellol with an incubation time of 48 hours were treated on HeLa cells. A viability test was carried out by WST-1 test and apoptosis detection was carried out by Annexin V-FITC/PI staining using a fluorescence microscope. The results of the one-way ANOVA test showed that there was no significant effect of different concentrations of citronellol on the viability of HeLa cells. The concentrations of 10 µg/mL of citronellol tended to decrease HeLa cells viability. Observations with fluorescence microscope showed a higher detection rate of apoptotic cells than necrotizing cells and a more visible morphological changes in late apoptotic cells. Furthermore, fluorescence microscopy detected apoptotic cells. Further study is required to get a more comprehensive information on the effects of citronellol on HeLa cells.

Abstrak :
Kanker serviks menjadi kanker utama penyebab kematian di Afrika Timur dan Tengah. Terdapat 266.000 kematian tiap tahunnya akibat kanker serviks diseluruh dunia. Sekitar 9 dari 10 wanita di negara berkembang mengalami kematian akibat kanker serviks. Di Indonesia terdapat 91.682 kasus kanker serviks selama kurun waktu 2010 hingga 2013. Alga merah Eucheuma denticulatum yang banyak di temui di perairan Indonesia telah diketahui memiliki aktivitas sebagai agen farmatikal, salah satunya sebagai agen antikanker. Penelitian ini bertujuan untuk menganalisis komponen fitokimia dan mengetahui aktivitas antikanker Eucheuma denticulatum terhadap sel kanker serviks HeLa. Pada penelitian ini, Eucheuma denticulatum diekstraksi menggunakan empat pelarut yang berbeda, yaitu etanol, etil asetat, n-heksana dan kloroform, menghasilkan masing-masing ekstrak etanol, ekstrak etil asetat, ekstrak n-heksana dan ekstrak kloroform Eucheuma denticulatum. Hasil uji fitokimia pada keempat jenis ekstrak Eucheuma denticulatum positif menunjukkan adanya metabolit sekunder. Sedangkan uji kromatografi lapis tipis menunjukkan bahwa ekstrak Eucheuma denticulatum ini mengandung 10 komponen senyawa. Selanjutnya, dilakukan uji aktivitas antikanker pada keempat jenis ekstrak Eucheuma denticulatum tersebut terhadap sel kanker serviks HeLa dengan menggunakan metode MTT assay, aktivitas antikanker dinyatakan dengan nilai IC50. Hasil uji aktivitas antikanker menunjukkan bahwa keempat ekstrak Eucheuma denticulatum memiliki aktivitas inhibisi terhadap sel kanker serviks HeLa dengan nilai IC50

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Cervical cancer is the leading cause of death in East and Central Africa. There are 266,000 deaths annually from cervical cancer worldwide. About 9 out of 10 women in developing countries die of cervical cancer. In Indonesia there are 91,682 cases of cervical cancer during the period 2010 to 2013. Eucheuma denticulatum red algae that many encountered in Indonesian waters has been known to have activity as a pharmaceutical agent, one of them as an anticancer agent. This study aims to analyze phytochemical components and to know the activity of anticancer Eucheuma denticulatum against cervical cancer cells HeLa. In this study, Eucheuma denticulatum was extracted using four different solvents, ie ethanol, ethyl acetate, n hexane and chloroform, yielding each ethanol extract, ethyl acetate extract, n hexane extract and chloroform Eucheuma denticulatum extract. The result of phytochemical test on four types of Eucheuma denticulatum positive extract showed secondary metabolite. While thin layer chromatography test showed that this Eucheuma denticulatum extract contains 10 components of compound. Furthermore, the anticancer activity test on all four types of Eucheuma denticulatum extract to cervical cancer cells HeLa by using MTT assay method, anticancer activity expressed with IC50 value. The results of the anticancer activity test showed that the four Eucheuma denticulatum extracts had inhibitory activity against heLa cervical cancer cell with IC50 value

Abstrak :
Doksorubisin (DOX) merupakan agen kemoterapi antrasiklin yang bekerja dengan memperlambat atau menghentikan pertumbuhan sel kanker. Namun, penggunaan DOX memiliki efek toksik pada sel normal serta efek samping kardiotoksisitas. Penelitian ini bertujuan membuat sistem penghantaran DOX dengan SPION-PAMAM-HA, mengkarakterisasi, dan mengevaluasi konjugat superparamagnetic iron oxyde nanoparticle-poliamidoamin G4-asam hialuronat-doksorubisin (SPION-PAMAM-HA-DOX). Hasil karakterisasi SPION-TC menunjukkan kristal magnetit dengan ukuran 2,79 nm. Konjugat SPION-PAMAM-HA-DOX menunjukkan diameter rata-rata 292,57 ± 4,86 nm, indeks polidispersitas 0,216 ± 0,002 nm, dan nilai zeta potensial -53,37 ± 3,68 mV. Studi pelepasan pada 10 jam dapat terlihat bahwa sebanyak 99% doksorubisin dilepaskan di lingkungan pH 5, sementara kurang dari 10% doksorubisin dilepaskan di lingkungan pH 7,4. Uji sitoksisitas menunjukkan konjugat SPION-PAMAM-HA-DOX memiliki efek toksik pada sel HeLa dan sel Vero dengan IC50 2,54 ± 0,27 µM pada sel HeLa dan CC50 2,18 ± 0,019 µM pada sel Vero serta memiliki nilai SI SPION-PAMAM-HA-DOX lebih besar dibandingkan SI DOX yang menandakan bahwa konjugat SPION-PAMAM-HA-DOX masih aman digunakan dan masih memiliki efek anti kanker. ......Doxorubicin (DOX) is an anthracycline chemotherapy agent that works by slowing or stopping the growth of cancer cells. However, DOX has a toxic effect on normal cells as well as a side effect of cardiotoxicity. This study aims to develop a DOX delivery system with SPION-PAMAM-HA, characterize, and evaluate of superparamagnetic iron oxide nanoparticle-polyamidoamine G4-hyaluronic aciddoxorubicin (SPION-PAMAM-HA-DOX). Superparamagnetic iron oxide nanoparticle (SPION) could be used as diagnostic agent, polyamidoamine G4 (PAMAM) was used as carrier and hyaluronic acid (HA) was used as active targeting moiety. SPION were prepared by co-precipitation method, then SPION-PAMAM-HA-DOX were produced by conjugation of SPION with PAMAM-HA and DOX. SPION-PAMAM-HA-DOX were characterized by particle size analyzer (PSA), UV-Vis spectrophotometry, fourier transform infrared spectroscopy (FTIR), fluorescence spectrophotometry, and transmission electron microscopy (TEM). The release study was conducted by using pH 5 and pH 7.4. Cytotoxicity study was conducted on HeLa cells and Vero cells by using MTT [3-(4,5-Dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] assay. The characterization results of SPION-TC showed magnetite crystals with a size of 2.79 nm. The mean particle size, polydispersity index, and zeta potential of SPION-PAMAM-HADOX were 57 ± 4.86 nm, 0.216 ± 0.002 nm, and -53.37 ± 3.68 mV. The release study at 10 hours showed that 99% of DOX was released in pH 5, while less than 10% DOX was released in pH 7.4. The cytotoxicity study showed that SPION-PAMAM-HA-DOX has toxicity effect on HeLa cells and Vero cells with IC50 2,54 ± 0,27 µM on HeLa cells, CC50 2,18 ± 0,019 µM on Vero cells sel Vero, and SI value of SPION-PAMAM-HA-DOX greater than SI DOX which indicates that SPION-PAMAM-HA-DOX conjugate is still safe to use and has anti-cancer effects

Abstrak :
Latar belakang: Kanker serviks merupakan kanker dengan prevalensi terbanyak keempat pada wanita di dunia, sehingga tatalaksana yang tepat dengan efek samping minimal sangat dibutuhkan. Salah satu tatalaksana yang sedang dikembangkan adalah terapi menggunakan herbal jahe (Zingiber officinale) yang diketahui memiliki aktivitas antioksidan dan sitotoksik. Penelitian ini bertujuan untuk mengetahui perbandingan aktivitas antioksidan dan sitotoksik ekstrak air rebusan jahe dan minyak esensial jahe. Metode: Ekstrak jahe yang diujikan adalah ekstrak air rebusan jahe dan minyak esensial jahe. Aktivitas antioksidan ekstrak jahe ditentukan menggunakan metode DPPH [2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl], sedangkan aktivitas sitotoksiknya terhadap sel kanker serviks HeLa ditentukan dengan metode MTT (3-(4,5-dimetilthiazol-2-yl)-2,5 difenil tetrazolium bromida) assay. Hasil: Ekstrak air rebusan jahe dan minyak esensial jahe menunjukkan aktivitas antioksidan terhadap DPPH yang tergolong aktif. Minyak esensial jahe dengan nilai IC50 sebesar 51,33 µg/mL, memiliki aktivitas antioksidan yang lebih baik daripada ekstrak air rebusan jahe dengan nilai IC50 sebesar 91,79 µg/mL. Aktivitas sitotoksik ekstrak air rebusan jahe (IC50=7,33 µg/mL) dan minyak esensial jahe (IC50=7,17 µg/mL) terhadap sel kanker serviks HeLa tergolong aktif dan tidak menunjukkan perbedaan yang bermakna secara statistik. Kesimpulan: Ekstrak air rebusan jahe dan minyak esensial jahe memiliki potensi unutk dikembangkan lebih lanjut sebagai antioksidan dan agen antikanker untuk terapi pengobatan kanker serviks. ......Introduction: Cervical cancer is cancer with the fourth most prevalence in women in the world, therefore the proper management with minimal side effects is needed. One of the treatments being developed is therapy using ginger (Zingiber officinale) which is known previously to have antioxidant and cytotoxic activity. This study aims to compare the antioxidant and cytotoxic activity of ginger boiled-water extract and ginger essential oil. Method: The ginger extracts tested were ginger boiled-water extract and ginger essential oil. The antioxidant activity of ginger extracts was measured by the DPPH [2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl] method, whereas cytotoxic activities of the extracts against HeLa cervical cancer cells were evaluated by the MTT (3-(4,5-dimethylthiazol-2- yl)-2,5 diphenyl tetrazolium bromide) assay. Result: Ginger boiled-water extract and ginger essential oil showed an active antioxidant activity against DPPH free radical. Ginger essential oil with an IC50 value of 51.33 g/mL, has a greater better antioxidant activity than ginger boiled-water extract with an IC50 value of 91.79 g/mL. Ginger boiled-water extract (IC50=7.33 g/mL) and ginger essential oil (IC50=7.17 g/mL) were classified to have an active cytotoxic activity against HeLa cervical cancer cells, and both of extracts did not show a statistically significant difference. Conclusion: Ginger boiled-water extract and ginger essential oil both have are potential to be developed for as cervical cancer therapy.

Abstrak :
Penelitian ini menyintesis senyawa amida turunan asam risinoleat dengan amilamina yaitu senyawa 12-hidroksi-N-pentilstearamida. Pertama metil risinoleat dipurifikasi menjadi metil risinoleat yang lebih murni. Kemudian produk setelah pemurnian dilanjutkan dengan reaksi hidrogenasi menggunakan gas hidrogen dan katalis Pd/C 10%. Setelah itu senyawa metil-12-hidroksistearat yang terbentuk dipurifikasi kembali dan dilakukan reaksi amidasi dengan amilamina. Senyawa lipoamida yang diperoleh dipurifikasi dengan kromatografi kolom dan struktur lipoamida yang diperoleh telah dikonfirmasi dengan spektrum FTIR. Hasil FTIR menunjukan bahwa terdapat gugus fungsi amida pada sampel, ditandai dengan adanya spektrum serapan ulur N-H dan O-H yang overlapping pada bilangan gelombang 3650-2400 cm-1 dan serapan kuat pada puncak C=O amida pada bilangan gelombang 1652 cm-1. Senyawa lipoamida tersebut diuji aktivitas antikankernya terhadap sel HeLa menggunakan metode MTT. Hasilnya menunjukan bahwa aktivitas sitotoksik senyawa 12-hidroksi-N-pentilstearamida tergolong sitotoksik sedang dengan nilai IC50 yang sebesar 37,73 µM. ......This research, an amide compound derived from ricinoleic acid was synthesized with amylamine, namely 12-hydroxy-N-pentylstearamide. First, methyl ricinoleate is purified to make methyl ricinoleate which is purer. Then the product after purification is followed by a hydrogenation reaction using hydrogen gas and 10% Pd/C catalyst. After that, the methyl-12-hydroxystearate formed was purified again and amidation reaction with amylamine. The lipoamide compound obtained was purified by column chromatography and the structure of the lipoamide obtained was confirmed by FTIR spectrum. The FTIR results showed that there was an amide functional group in the sample, indicated by the presence of overlapping N-H and O-H stretching absorption spectra at wave numbers 3650-2400 cm-1 and strong absorption at the amide C=O peak at wave numbers 1652 cm-1. The lipoamide compounds were tested for their anticancer activity against HeLa cells using the MTT method. The results showed that the cytotoxic activity of 12-hydroxy-N-pentylstearamide was moderate with an IC50 value of 37,73 µM.

Abstrak :
Kanker serviks merupakan kanker dengan jumlah kasus tertinggi kedua di Indonesia. Banyaknya jumlah kasus kanker di Indonesia serta kasus kematian akibat kanker menyebabkan diperlukannya pengembangan obat-obatan untuk menyembuhkan kanker. Pengobatan yang ada dinilai cukup efektif tetapi memiliki beberapa efek samping yang berbahaya, sehingga diperlukan juga pengembangan obat antikanker dari bahan-bahan alami seperti ekstrak tumbuhan. Minyak esensial akar wangi memiliki potensi antikanker, akan tetapi pengaruh konsentrasi minyak esensial akar wangi perlu diteliti. Tujuan dari penelitian adalah untuk mengetahui pengaruh variasi konsentrasi minyak esensial akar wangi terhadap sel HeLa. Sel HeLa diberikan empat perlakuan dengan variasi konsentrasi (5 µg/mL, 10 µg/mL, 15 µg/mL, dan 20 µg/mL) minyak esensial akar wangi dan dilakukan pengulangan sebanyak lima kali. Viabilitas sel diuji menggunakan WST-1 dan Scepter cell counter. Nilai viabilitas pada metode WST-1 dihitung berdasarkan nilai absorbansi, sedangkan nilai viabilitas pada metode Scepter cell counter dihitung berdasarkan ukuran diameter sel. Semakin tinggi nilai absorbansi, maka semakin tinggi juga nilai viabilitasnya. Untuk ukuran diameter, sel HeLa yang viable memiliki rentang diameter 12—14 µm. Hasil penelitian menunjukkan tiap variasi konsentrasi minyak esensial akar wangi memiliki efek yang tidak signifikan terhadap viabilitas sel HeLa. Namun, minyak esensial akar wangi 15 µg/mL dan 20 µg/mL memiliki kecenderungan paling besar dalam menurunkan viabilitas sel HeLa berdasarkan hasil uji viabilitas dengan WST-1 dan Scepter cell counter. Kesimpulan dari penelitian adalah minyak esensial akar wangi tidak memberikan pengaruh yang signifikan terhadap viabilitas sel HeLa, akan tetapi terdapat konsentrasi tertentu yang memiliki kecenderungan dalam menurunkan viabilitas sel HeLa, yaitu konsentrasi 15 µg/mL dan 20 µg/mL. ...... Cervical cancer is the cancer with the second highest number of cases in Indonesia. The large number of cancer cases in Indonesia as well as cases of death due to cancer make it necessary to develop drugs to cure cancer. Existing treatments are considered quite effective but have several dangerous side effects, so it is also necessary to develop anticancer drugs from natural ingredients such as plant extracts. Vetiver essential oil has anticancer potential, but the effect of vetiver essential oil concentration needs to be studied. The aim of the research was to determine the effect of varying concentrations of vetiver essential oil on HeLa cells. HeLa cells were given four treatments with varying concentrations (5 µg/mL, 10 µg/mL, 15 µg/mL, and 20 µg/mL) of vetiver essential oil and repeated five times. Cell viability was tested using WST-1 and Scepter cell counter. The viability value in the WST-1 method is calculated based on the absorbance value, while the viability value in the Scepter cell counter method is calculated based on the size of the cell diameter. The higher the absorbance value, the higher the viability value. In terms of diameter, viable HeLa cells have a diameter range of 12—14 µm. The results showed that each variation in vetiver essential oil concentration had an insignificant effect on HeLa cell viability. However, 15 µg/mL and 20 µg/mL vetiver essential oil had the greatest tendency to reduce HeLa cell viability based on the results of viability tests with WST-1 and Scepter cell counter. The conclusion of the research is that vetiver essential oil does not have a significant effect on HeLa cell viability, but there are certain concentrations that have a tendency to reduce HeLa cell viability, namely concentrations of 15 µg/mL and 20 µg/mL.

Abstrak :
Telah dilakukan sintesis enam senyawa analog kurkumin monokarbonil asimetrik AKMA dengan menggunakan reaksi kondensasi Clasein-Schmidth, dimana reaksi tersebut dilakukan dalam dua tahapan, tahap pertama menggunakan basa sebagai katalisdan menggunakan katalis asam pada tahap kedua. Rendemen senyawa AKMA yang diperoleh berturut-turut untuk senyawa 1a, 1b, 1c, 1d, 1e, dan 1f adalah 50,03 ; 2,70 ;47,31 ; 6,10 ; 13,16 ; 55,53. Substitusi basa Mannich dietilamin terhadap senyawa AKMA yang telah disintesis dilakukan melalui reaksi substitusi nukleofilik, diperoleh senyawa 2a, 2b, 2c, 2d, 2e, dan 2f dengan rendemen berturut-turut 65, 51 ; 46,78 ; 33,01 ; 76,93 ; dan 50,35. Elusidasi struktur dari seluruh senyawa yang telah disintesis dilakukan dengan instrumen FT-IR, HRMS, 1H-NMR dan 13C-NMR, sementara itu untuk kemurnian senyawa dianalisis berdasarkan pengujian jarak lebur. Aktivitas sitotoksik dari seluruh senyawa yang berhasil disintesis diuji dengan menggunakan metode MTT pada sel kanker HeLa. Hasil MTT seluruh senyawa hasil sintesis terhadap sel HeLa menunjukan jika seluruh senyawa memiliki kemampuan untuk menghambat pertumbuhan sel HeLa, dan substitusi basa Mannichdietilamin kedalam molekul AKMA dapat meningkatkan aktivitas inhibisi pertumbuhan sel HeLapada senyawa 2a, 2b, 2c, 2d, dan 2f. ......Synthesis of six analogous of the asymmetric monocarbonyl analogous of curcumin asymmetric MACs using Clasein Schmidt condensation reaction were done in two stages, the first stage were using a base as a catalyst and using an acid catalyst in the second stage. The yield of the compound of asymmetric MACs obtained successively for the compounds 1a, 1b, 1c, 1d, 1e, and 1f was 50.03 2.70 47.31 6.10 13.16 55.53. Substitution of diethylamine Mannich base into synthesized asymmetric MACs compound were carried out by a nucleophilic substitution reaction, obtained bycompounds 2a, 2b, 2c, 2d, 2e, and 2f with a successive yield of 65.51 46.78 33.01 76.93 and 50.35. All of compound's structure were elucidated by FT IR, HRMS, 1HNMR and 13C NMR instruments, mean while for the purity of the compounds were analyzed based on melt range testing. The cytotoxic activity of all successfully synthesized compounds were tested using MTT method on HeLa Cells line. The MTT result of all compounds againts HeLa cells indicates if they have ability to in hibit the growth of HeLa cells lines, and substitution of diethylamine Mannich base into asymmetric MACs molecules can increase the activity of inhibition of HeLa cell grow thon compound 2a, 2b, 2c, 2d, and 2f.