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Abstrak :
Latar Belakang: Perubahan genetik pada p53 menyebabkan imortalisasi dan kecenderungan sel bertransformasi menjadi neoplasma. Imortalisasi ini berhubungan dengan pemeliharaan panjang telomer oleh telomerase. hTERT adalah komponen kunci telomerase yang aktivitasnya ditekan oleh p53. Tujuan: Menganalisis profil protein hTERT pada sel galur KSSRM HSC-3 dan HSC-4 serta jaringan mukosa mulut normal. Metode: Profil protein hTERT dianalisis menggunakan teknik SDS-PAGE dan Gel Doc, Quantity One. Hasil: Protein hTERT diekspresikan oleh sel galur KSSRM mulut tipe HSC 3 dan HSC 4 serta 2 dari 17 sampel protein jaringan mukosa mulut normal. Simpulan: Protein hTERT yang diekspresikan oleh sel galur KSSRM berhubungan dengan kondisi mutan p53. Adanya ekspresi protein hTERT pada jaringan mukosa mulut normal diperkirakan karena adanya sel keratinosit dan infiltrasi sel hematopoietik.

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Backround: Genetic alteration on p53 allows cellular immortalization and predisposes cells to neoplastic transformation. This immortalization is related to telomere length maintenance by telomerase. hTERT is a key component of telomerase, which activity is suppressed by p53. Objectives: To analyze the hTERT protein profile in HSC-3 and HSC-4 OSCC cell lines and normal human oral mucosa tissue. Methods: SDS-PAGE and Gel Doc, Quantity One were used for analyzing hTERT protein profile. Results: hTERT protein expressed in HSC-3 and HSC-4 OSCC cell lines and 2/17 protein samples of normal human oral mucosal tissues. Conclusion: hTERT protein that was expressed by OSCC cell lines is related to their status of mutant p53. The existing of hTERT protein on normal human oral mucosas tissue may be caused by keratinocyte cells and infiltrated hemapoietic cells.

Abstrak :
Latar Belakang: p73, homolog p53, diketahui memiliki kemampuan serupa dalam menekan pertumbuhan tumor. Protein p73 diekspresikan dalam berbagai level pada sel kanker dan jaringan normal yang berbeda. Belum diketahui bagaimana pola ekspresi protein p73 pada KSSRM dan pada jaringan mukosa mulut normal. Tujuan: Mengetahui profil protein p73 pada KSSRM tipe mutant p53 dan jaringan mukosa mulut normal berdasarkan berat molekul protein. Metode: Ekstrak protein dari HSC-3 dan HSC-4 serta jaringan mukosa normal dianalisa dengan teknik SDS PAGE untuk mendeteksi protein p73 berdasarkan berat molekulnya. Hasil:. pita protein p73 pada HSC-3 lebih tebal daripada HSC- 4. Terdapat variasi profil protein p73 pada mukosa mulut normal dengan pita protein tebal (8/17) dan sedang (5/17). Simpulan: Terdapat perbedaan profil protein p73 antara HSC-3 dan HSC-4 berkaitan dengan tingkat protein p53 dan SNP pada kodon 72. Kebanyakan sampel jaringan mukosa memperlihatkan ketebalan pita protein p73 yang cukup tinggi. ......Backround: p73, the homolog of p53, has a similar ability in tumor suppression. p73 protein expressed at a different level in various cancer cells and normal tissues. Profile of p73 protein in mutant p53 OSCC cell line and normal human oral mucosa have not been known. Objectives: To observe p73 protein profile in mutant p53 OSCC cell lines and normal human oral mucosa. Methods: The extracted protein of HSC-3 and HSC-4 cell lines and normal mucosal tissues were analyzed with SDS PAGE to detect p73 protein based on the molecular weight. Results:. The band of p73 protein in HSC-3 shows a higher density compared to its density of HSC-4. A thick p73 protein band was shown on 8/17 of normal mucosal tissues while medium level of p73 protein band was shown on 5/17. Conclusion: The protein profile between HSC-3 and HSC-4 were different related with p53 protein and SNP on codon 72 of each samples. Most of mucosal tissues shows a quite high density of p73 protein bands.

Abstrak :
Kanker mulut berada diurutan 32 dunia penyebab kematian yang paling sering terjadi. Pada beberapa penelitian, kitosan berefek pada beberapa jenis sel baik menaikkan maupun menurunkan viabilitas sel. Penelitian ini bertujuan untuk mengetahui efek kitosan terhadap galur sel kanker skuamosa mulut (HSC-4) dan sel epitel dental (HAT-7) in vitro dan dipajan kitosan pada konsentrasi 0,0005%; 0,0025%; 0,005%; 0,25%; 0,5%. Kemudian hasilnya diukur dengan 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay atau MTT assay. Viabilitas pada kedua jenis sel lebih tinggi pada konsentrasi 0,0005%; 0,0025%; 0,005%, sedangkan pada konsentrasi 0,25% dan 0,5% lebih rendah dibandingkan kontrol. Kitosan memiliki efek pada kedua jenis sel bergantung pada konsentrasinya.

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Oral cancer ranked 32th as most causes of death in the world. From past research, chitosan has inhibit proliferation effect. The present study examined the effect of chitosan to oral cancer squamous carcinoma cell line (HSC-4) and dental epithelial cell line (HAT-7) in vitro. Both cells were exposed by chitosan with concentration 0,0005%; 0,0025%; 0,005%; 0,25%; and 0,5%. Then, the result was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay or MTT assay. Both cells has higher viability at 0,000%; 0,0025%; and 0,005%, whereas lower viability showed at 0,25% and 0,5%. Chitosan has effect on both cells depends on concentration.

Abstrak :
Protein sel merupakan makromolekul yang terdiri dari satu atau beberapa polipeptida yang tersusun dari rangkaian asam amino yang saling berikatan. Terdapat perbedaan profil protein antara sel normal dengan sel kanker. Tujuan : Melihat ekspresi protein pada KSSRM dan mukosa mulut normal. Metode : Sel galur HSC-3 dan HSC-4 dikultur hingga confluent. Sel skuamosa mukosa normal diambil dari jaringan gingiva pasien odontektomi. Semua sampel dilakukan prosedur ekstraksi protein, Bradford protein assay, dan SDS PAGE. Hasil : KSSRM mengekspresikan protein dengan level cukup tinggi pada berat molekul 31-78 kDa. Namun, pada mukosa normal, kebanyakan mengekspresikan protein pada berat molekul antara 39 - 172 kDa. Kesimpulan : Terdapat perbedaan ekspresi protein pada sel galur KSSRM dibandingkan dengan mukosa mulut normal.

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Cells Proteins are macro molecules consist of one or several polypeptides which formed from amine acid chain that bound one another. There is a different of protein profile between normal and cancer cells. Objective : To observe the protein expression in OSCC and normal oral mucous. Method : Cell lines HSC-3 and HSC-4 were cultured until confluent. Normal squamous mucosa was taken from gingival tissues patient who had odontectomy procedure. Protein extraction, Bradford protein assay, and SDS PAGE procedure were performed for all samples. Results : Oral squamous cells carcinoma expressed rather high level of protein which have molecular weight of 31-78 kDa compared to normal gingival which express protein molecular weight ranging between 39 - 172 kDa. Conclusion : There are different protein expression between oral squamous cells carcinoma and normal oral mucous.