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"Plant quality improvement, as a source of oil, was one of the objective of oil palm plant breeding program, such as genetic engineering. Palmitoyl-ACP thioesterase (PAT) gene was an enzyme code, which influenced the synthesis of palmitic acid, where its existence was unexpected in crude palm oil (CPO), due to its negative impact on human health. The first step in genetic engineering was isolating the gene which related to the plant's negative trait. The gene silencing phase was expected to avoid the expression of gene PAT, to reduce the proportion of palmitic acid as well as increasing the content of oleic acid, which was expected to present in CPO.The aim of the research was to identify nucleoride sequence of the PAT gene of the oil palm. The procedure of the research included, extraction the DNA from leaves, gene isolation through specific primer design and amplification, cloning the PAT gene including ligation and transformation, a series of confirmation and verification, and PAT gene sequencing.The result showed that 2 fragments of DN A had been isolated by using 2 different pair of primers, they was PAT I and PAT II fragment. PAT I gene fragment was amplified by using PAT II and PAT TF primers. Recombinant plasmid carrying PAT I gene fragment had been produced through cloning, ligation and transformation inside the strain of DH5a Escherichia coli. The targeted fragment inside the plasmid then was sequence and producing 1,063 bp fragment. 750 bp length PAT II gene, was amplified by PAT U and PAT UR primer."

"Tandan kosong kelapa sawit (Elaeis guineensis Jacq). merupakan limbah dari proses pengolahan Elaeis guineensis yang berpotensi sebagai bahan pakan ternak. Penelitian bertujuan untuk mengetahui kemampuan Phanerochaete chrysosporium Burds.—biakan laboratorium Bioteknologi, BPPT, Serpong— dalam mendegradasi tandan kosong kelapa sawit. Penelitian bersifat eksperimental dengan Rancangan Acak Lengkap (RAL). Parameter hasil degradasi yang diamati adalah Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), lignin, aktivitas lignin peroksidase (LiP) dan mangan peroksidase (MnP). Fermentasi dilakukan dengan substrat padat secara still culture. Pengujian kadar NDF, ADF, lignin menggunakan metode Apriantono dkk. 1989 dan aktivitas enzim LiP serta MnP diuji menggunakan metode Fujian dkk. 2001 Hasil uji statistik anova dua faktor menunjukkan bahwa terdapat perbedaan yang signifikan antara kelompok kontrol dan perlakuan pada kadar NDF, ADF, dan lignin (α=0,05). Hasil pengukuran kadar rata-rata NDF pada kelompok perlakuan dari minggu ke-0 sampai ke-4, secara berturut-turut, adalah 0,787 g; 0,778 g; 0,774 g; 0,766 g; dan 0,761 g. Penurunan kadar rata-rata NDF selama 4 minggu adalah 40,62%. Hasil pengukuran kadar rata-rata ADF pada kelompok perlakuan dari minggu ke-0 sampai ke-4, secara berturut-turut, adalah 0,572 g; 0,565 g; 0,439 g; 0,358 g; dan 0,327 g. Penurunan kadar rata-rata ADF selama 4 minggu adalah 42,531%. Hasil pengukuran kadar rata-rata lignin padakelompok perlakuan dari minggu ke-0 sampai ke-4, secara berturut-turut, adalah 0,154 g; 0,141 g; 0,113 g; 0,063 g; dan 0,053 g. Total penurunan kadar rata-rata lignin selama 4 minggu adalah 65,359%. Hasil aktivitas enzim LiP dan MnP (U/ml) yang dihasilkan dari minggu ke-1 sampai ke-4, masing-masing, adalah 48,9 dan 134,01; 77,59 dan 163,62 ; 82,15 dan 181,70; serta 82,77 dan 186,92."