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Abstrak :
Latar Belakang: Berdasarkan data dari WHO, penderita penyakit kardiovaskular diduga akan terus meningkat. Salah satu proses patologis yang mendasari penyakit kardiovaskular adalah aterosklerosis. Disfungsi endotel yang mengawali aterosklerosis dimulai sejak anak-anak. Stres oksidatif dapat disebabkan oleh pertambahan usia. Salah satu herba yang memiliki efek antioksidan kuat dan dapat mencegah stres oksidatif adalah Hibiscus sabdariffa Linn. Metode: Penelitian eksperimental dilakukan pada 36 ekor tikus jantan galur Wistar usia 5 minggu selama 4 minggu, 8 minggu, dan 12 minggu. Hewan coba secara acak terbagi atas 12 kelompok, yaitu: kontrol (K4, K8, K12), latihan fisik aerobik (L4, L8, L12), pemberian H. sabdariffa Linn. 400 mg/kgBB/hari (H4, H8, H12) dan kombinasi latihan fisik aerobik dan pemberian H. sabdariffa Linn. 400 mg/kgBB/hari (HL4, HL8, HL12). Pengukuran kadar NO, ET-1, aktivitas spesifik SOD dan MDA menggunakan supernatan dari homogenat aorta abdominal. Hasil: Pola kadar NO kelompok K dan L menurun sesuai peningkatan usia. Terdapat perbedaan bermakna antara kadar NO kelompok K dan L, K dan H, dan K dan HL. Kadar ET-1 pada semua kelompok tidak bermakna secara statistik. Terdapat peningkatan aktivitas spesifik SOD pada kelompok L, H, dan HL dibandingkan K. Terdapat perbedaan bermakna Kadar MDA antara K dan H, L dan HL. Terdapat korelasi sedang antara NO dan aktivitas spesifik SOD. Kesimpulan: latihan fisik aerobik, pemberian H. sabdariffa Linn. 400 mg/kgBB/hari dan kombinasi latihan fisik aerobik dan pemberian H. sabdariffa Linn. 400 mg/kgBB/hari menurunkan kadar MDA dan ET-1, sebalikanya meningkatkan aktivitas spesifik SOD dan NO. Penurunan kadar MDA lebih jelas terlihat pada kelompok HL. Peningkatan aktivitas spesifik SOD meningkatkan produksi NO. Tidak terjadi disfungsi endotel dan stres oksidatif pada seluruh kelompok. ...... Background: Based on data from WHO, patients with suspected cardiovascular disease will continue to rise. One of the pathological processes underlying cardiovascular disease is atherosclerosis. Endothelial dysfunction which is the first sign of atherosclerosis begins in childhood. Increasing age is one of the cause of oxidative stress. A herb that has strong antioxidant effects and can prevent oxidative stress is Hibiscus sabdariffa Linn. Methods: Thirty six male Wistar rats aged 5 weeks were randomly divided into 12 groups consisting of control group (K4, K8, K12), aerobic exercise group (L4, L8, L12), administration of H. sabdariffa L. 400 mg/kgBW/day group (H4, H8, H12) and combination of aerobic exercise and H. sabdariffa L. 400 mg/kgBW/day group (HL4, HL8, HL12). NO, ET-1, MDA level, and SOD activity was measured from abdominal aorta homogenate supernatant. Results: NO level pattern in the K and L groups tend to decline with age. NO level in L, H and HL groups were higher than K. The difference of ET-1 level in all groups were not statistically significant. Specific activity of SOD in L, H and HL groups were higher than control. The concentration of MDA of group K is significantly lower compare to groups H, L and HL. There is a moderate correlation between specific activity of SOD and NO. Conclutions: Aerobic exercie, administration of H. sabdariffa L. 400 mg/kgBW/day, and combination of both decreases MDA and ET-1 concentration. While, specific activity of SOD and NO are increased. The decrease at MDA concentration was more prominent in HL group. An increase in spesific activity of SOD, increases the NO level. No endothelial dysfunction nor oxidative stress were observed in all groups.

Abstrak :
Latar belakang: Cisplatin diketahui sebagai agen kemoterapi yang paling sering digunakan dalam terapi keganasan hematologi dan tumor padat. Namun, efek samping cisplatin nefrotoksisitas menjadi masalah utama, sebab pemberian cisplatin sesuai dengan dosis terapeutik saja sudah menimbulkan kerusakan ginjal. Tujuan penelitian: Membuktikan efek kurkumin yang bersifat renoprotektif terhadap kerusakan ginjal yang diinduksi cisplatin melalui penurunan ekspresi endothelin-1 (ET-1). Endothelin-1 merupakan peptida yang berkaitan dengan fungsi ginjal, karena memiliki peran sebagai vasokonstriktor poten dan sebagai hemodinamik di organ ginjal. Metode: Sampel yang digunakan dalam penelitian ini berupa organ tersimpan dari penelitian Ni Made Dwi Sandhiutami, S.Si, M.Kes, Apt. Sampel dibagi dalam 4 kelompok perlakuan, yaitu kelompok tikus normal (N), kanker ovarium tanpa perlakuan (Ca), kanker ovarium perlakuan cisplatin (Cis), dan kanker ovarium perlakuan cisplatin+curcumin (Cur). Induksi kanker ovarium dengan cara injeksi 7,12-Dimethylbenz[a]anthracene (DMBA) melalui benang silk dan dirawat selama 20 minggu membentuk adanya tumor pada ovarium. Hasil: Hasil yang diperoleh memperlihatkan kelompok Ca mengalami peningkatan ET-1 sebesar 2,8555±0,69981, kelompok Cis terjadi peningkatan ET-1 sebesar 6,0965±2,1558, namun pada kelompok Cis+Cur terjadi penurunan ekspresi ET-1 sebesar 2,1616±0,71623. Meskipun, data pada pemeriksaan ekspresi ET-1 tidak berbeda bermakna secara statistik, namun pemberian kurkumin dapat dikatakan bermakna secara kondisi klinis karena menurunkan ekspresi ET-1 sebesar 64,5%. Pada pemeriksaan serum kreatinin, hanya kelompok tikus normal (N) dengan kelompok tikus kanker ovarium dengan perlakuan cisplatin (Cis) yang berbeda bermakna dan signifikan. Kesimpulan: Cisplatin bersifat nefrotoksisitas meskipun dalam dosis terapi, serta pemberian kurkumin bermanfaat sebab dapat bersifat renoprotektif bagi kerusakan ginjal yang diinduksi cisplatin. ......Introduction: Cisplatin is known as a chemotherapy agent that is most often used in the therapy of hematologic malignancies and solid tumors. However, the side effects of cisplatin nephrotoxicity are the main problem, because giving cisplatin in accordance with the therapeutic dose alone has caused kidney damage. Purpose of the study: To prove the effect of curcumin which is renoprotective against cisplatin-induced renal damage through decreased expression of endothelin-1 (ET-1). Endothelin-1 is a peptide related to kidney function, because it has a role as a potent vasoconstrictor and as a hemodynamic agent in the kidney. Methods: The samples used in this study were stored organs from research by Ni Made Dwi Sandhiutami, S.Si, M.Kes, Apt. Samples were divided into 4 treatment groups, namely normal mice (N), ovarian cancer without treatment (Ca), ovarian cancer treated with cisplatin (Cis), and ovarian cancer treated with cisplatin + curcumin (Cur). Ovarian cancer induction by injection of 7,12-Dimethylbenz [a] anthracene (DMBA) through silk thread and treated for 20 weeks to form a tumor in the ovary. Results: The results showed that the Ca group experienced an increase in ET-1 by 2.8555 ± 0.69981, the Cis group had an increase in ET-1 by 6.0965 ± 2.1558, but in the Cis + Cur group there was a decrease in ET-1 expression. equal to 2.1616 ± 0.71623. Although, the data on the examination of ET-1 expression were not statistically significant, but curcumin administration can be said to be clinically significant because it reduces the expression of ET-1 by 64.5%. In the serum creatinine examination, only the normal (N) group of mice with ovarian cancer mice treated with cisplatin (Cis) was significantly and significantly different. Conclusion: Cisplatin is nephrotoxicity even in therapeutic doses, and curcumin administration is beneficial because it can be renoprotective for cisplatin-induced renal damage