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Abstrak :
Penyakit Parkinson disebabkan oleh kerusakan neuron penghasil dopamin di batang otak, yang menyebabkan gejala khas seperti tremor, kekakuan otot, dan gangguan keseimbangan. Pembuatan biosensor untuk mendeteksi kadar dopamin menjadi upaya penting dalam deteksi dini dan pengobatan Parkinson. Pada penelitian ini amperometrik biosensor screen printed carbon electrode (SPCE) dimodifikasi dengan reduced graphene oxide (rGO), polypyrrole (PPy), dan zinc oxide nanoparticle (ZnO-NP). Hasil modifikasi dibandingkan dengan SPCE yang belum dimodifikasi dalam pendeteksian dopamin. Karakterisasi material dilakukan dengan menggunakan scanning electron microscope (SEM), fourier transform infrared (FTIR), dan x-ray diffraction (XRD). Pengukuran elektrokimia dilakukan dengan metode cyclic voltammetry (CV). Hasil penelitian menunjukkan bahwa rGO/PPy/ZnO-NP/SPCE memiliki limit deteksi 0,0464 mM, sensitivitas sebesar 62,37 µA mM^-1 cm^-2 untuk jangkauan linear 0,01 – 1 mM ...... Parkinson's disease is caused by damage to dopamine-producing neurons in the brainstem, which causes characteristic symptoms such as tremors, muscle rigidity and balance disturbances. Making a biosensor to detect dopamine levels is an important effort in the early detection and treatment of Parkinson's. In this study the amperometric biosensor screen printed carbon electrode (SPCE) was modified with reduced graphene oxide (rGO), polypyrrole (PPy), and zinc oxide nanoparticle (ZnO-NP). Modified results were compared with unmodified SPCE in the detection of dopamine. Material characterization was carried out using a scanning electron microscope (SEM), fourier transform infrared (FTIR), and x-ray diffraction. Electrochemical measurements were carried out using the cyclic voltammetry (CV) method. The results showed that rGO/PPy/ZnO-NP/SPCE had a detection limit of 0.0464 mM, a sensitivity of 62.37 µA mM^-1 cm^-2 for a linear range of 0.01 – 1 mM.

Abstrak :
Dopamin memiliki peran penting dalam fungsi sistem saraf pusat manusia. Pelepasan abnormal dopamin berhubungan dengan penyakit neurologis dan depresi. Oleh karena itu, perlu memantau kadar dopamin untuk memahami peran fisiologisnya. Pembahasan deteksi dopamin berfokus pada metode elektrokimia berbasis screen-printed carbon electrode (SPCE) yang dimodifikasi bahan ZnO/MnO2/MWCNT. Penelitian dilakukan dengan empat tahapan yang terdiri dari preparasi material, karakterisasi material dengan menggunakan Fourier Transform Infrared (FTIR) dan Raman spektroskopi, modifikasi SPCE dengan ZnO/MnO2/MWCNT, dan pengujian aktivitas elektrokimia menggunakan cyclic voltametry (CV). Modifikasi sensor dilakukan untuk mengetahui perbedaan kinerja analitik biosensor SPCE. Tujuan dari penelitian ini adalah untuk mengetahui performa SPCE dengan pendopingan nanopartikel logam ZnO, MnO2, serta material karbon MWCNT berdasarkan linearitas, sensitivitas dan limit deteksi. Hasil pembacaan elektrokimia menggunakan CV dilakukan pada rentang deteksi 0,6 – 1,4 mM, sehingga diperoleh deteksi limit (LOD) 0,4946 mM dan sensitivitas 0,282 µA.µM.cm-2. Sensor ini menunjukkan selektivitas yang kurang baik terhadap analit dopamin ketika dideteksi bersama senyawa asam askorbat.

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The human central nervous system relies on dopamine to function properly. Neurological disorders and depression are linked to abnormal dopamine release. Monitoring dopamine levels is therefore crucial to comprehend its physiological function. The electrochemical approach of dopamine detection that utilises a screen-printed carbon electrode (SPCE) modified with ZnO/MnO2/MWCNT material is the main topic of discussion. The study was conducted in four stages, including material preparation, Fourier Transform Infrared (FTIR) and Raman spectroscopy material characterisation, SPCE modification using ZnO/MnO2/MWCNT, and cyclic voltametry (CV) electrochemical activity testing. To identify variations in SPCE biosensor analytical performance, sensor modifications were made. This study's goal was to evaluate the performance of SPCE doped with ZnO, MnO2, and MWCNT metal nanoparticles. The goal of this study was to evaluate the linearity, sensitivity, and limit of detection of SPCE doped with metal nanoparticles such as ZnO, MnO2, and MWCNT carbon materials. In order to achieve a detection limit (LOD) of 0,4946 mM and a sensitivity of 0,282 µA.µM.cm-2, electrochemical readings using CV were performed in the detection range of 0,6 - 1,4 mM. When this sensor is measured with interference-causing substances in the body such ascorbic acid, it exhibits poor selectivity for dopamine analytes.