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"Penelitian telah dilakukan pada oosit domba garut dengan menambahkan α-tokoferol ke dalam media vitrifikasi oosit dengan tujuan mengevaluasi penambahan α-tokoferol di dalam media vitrifikasi terhadap kualitas oosit domba garut. Oosit dikumpulkan & dimatangkan in vitro selama 24 jam pada suhu 38,5 ° C dan 5% CO2 kemudian di vitrifikasi menggunakan 15% EG, 15% DMSO dan sukrosa 0,5 M dan dibagi menjadi 4 kelompok: kelompok tanpa penambahan α- tokoferol (KK); dan tiga kelompok dengan penambahan berbagai konsentrasi α-tokoferol: 100 μM (KP1); 150 μM (KP2) dan 200 μM (KP3). Oosit disimpan dalam LN2 (-196 ° C) selama 7 hari dan dicairkan kembali setelahnya untuk mengevaluasi mereka menggunakan fluoresensi mikroskop dengan pewarna Hoechst & PI. Oosit normal jika mereka bulat, memiliki zona pelusida utuh, sitoplasma homogen; dan layak jika mereka memiliki warna biru pendaran. Evaluasi morfologi menunjukkan bahwa 69,40% (KK); 70.28% (KP1); 76,19% (KP2); dan 74,72% (KP3) dari total oosit vitrifikasi normal. Viabilitasnya evaluasi menunjukkan bahwa 75,52% (KK); 79,44% (KP1); 80,75% (KP2); 83,61% (KP3) dari total oosit vitrifikasi dapat hidup. Hasil statistik menunjukkan bahwa penambahan α- tokoferol dalam media vitrifikasi tidak memberikan perbedaan yang signifikan kelompok perlakuan (P> 0,05). Berdasarkan persentase, oosit normal tertinggi adalah diperoleh di KP2 dan oosit tertinggi di KP3. Hasil ini menunjukkan bahwa penambahan α-tokoferol dalam media vitrifikasi tidak berpengaruh terhadap kualitas oosit domba garut (P> 0,05).

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Research has been carried out on arrowroot oocytes by adding α-tocopherol to the oocyte vitrification media with the aim of increasing α-tocopherol funds in vitrification media on the quality of arrowroot sheep oocytes. Oocytes were collected & matured in vitro for 24 hours at 38.5 ° C and 5% CO2 then vitrified using 15% EG, 15% DMSO and 0.5 M sucrose and divided into 4 groups: groups without α-tocopherol changes (KK); and three groups contributing various α-tocopherol concentrations: 100 μM (KP1); 150 μM (KP2) and 200 μM (KP3). Oocytes are stored in LN2 (-196° C) for 7 days and thawed afterwards to return they used a fluorescence microscope with Hoechst & PI coloring. Oocytes are normal if they are round, have intact zona pellucida, homogeneous cytoplasm; and feasible if they have blue luminescence. Morphological evaluation showed that 69.40% (KK); 70.28% (KP1); 76.19% (KP2); and 74.72% (KP3) of total normal vitrified oocytes. Viability Assessment shows 75.52% (KK); 79.44% (KP1); 80.75% (KP2); 83.61% (KP3) of total vitrified oocytes can live. Statistical results showed that administration of α-tocopherol in vitrified media did not provide a significant difference in the treatment group (P> 0.05). Based on the percentage, the highest normal oocyte is obtained at KP2 and the highest oocyte is at KP3. These results indicate that opposing α-tocopherol in vitrified media has no effect on the quality of arrowroot sheep oocytes (P> 0.05)."

"Telah dilakukan penelitian untuk mengetahui pengaruh sari buah jeruk siam Citrus nobilis Lour. dalam pengencer tris kuning telur TKT terhadap kualitas spermatozoa domba garut Ovis aries L. 24 jam pascakriopreservasi. Semen ditampung dari lima pejantan domba garut satu kali dalam satu minggu menggunakan vagina buatan. Segera setelah dievaluasi, semen diencerkan dengan pengencer tris kuning telur yang mengandung sari buah jeruk 0 KK, 5 KP 1, 10 KP 2, 15 KP 3, dan 20 KP 4. Semen dikemas dalam straw 0,25 ml dengan konsentrasi akhir 50x106 spermatozoa/ml. Semen diekuilibrasi pada suhu 5oC selama dua jam kemudian dibekukan dan disimpan dalam tabung N2 cair. Semen dievaluasi terhadap parameter motilitas, viabilitas, integritas membran, integritas akrosom, dan abnormalitas. Hasil uji ANAVA satu arah menunjukkan terdapat perbedaan nyata antar kelompok perlakuan terhadap persentase motilitas, viabilitas, dan integritas membran p < 0,05. Hasil uji lanjut Duncan menunjukkan bahwa KP 2 memiliki perbedaan nyata terhadap semua kelompok perlakuan. Berdasarkan hasil tersebut, penambahan sari buah jeruk pada konsentrasi 10 KP 2 merupakan konsentrasi terbaik yang mampu memperkecil penurunan kualitas spermatozoa domba garut pascakriopreservasi berdasarkan persentase motilitas 58,68 0,68, viabilitas 59,73 6,47, dan integritas membran 54,87 5,58.

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The research aimed to find out the effect of siam orange juice in extender on garut ram spermatozoa quality 24 hours postcryopreseration. Semen was collected from five rams once a week using artificial vagina. Semen sample was diluted in tris egg yolk based extender containing 0 KK, 5 KP 1, 10 KP 2, 15 KP 3 and 20 KP 4 siam orange juice. Semen was packed in 0.25 ml straw with a final concentration of 50x106 spermatozoa ml. Sperm was equilibrated at 5 C for two hours then frozen and stored in a liquid nitrogen N2 tube. Sperm parameters including motility, viability, membrane integrity, acrosome integrity, and abnormality were assessed. One way ANAVA test results showed significant differences between treatment groups on the percentage of motility, viability, and membrane integrity."

"Telah dilakukan penelitian untuk mengetahui pengaruh berbagai konsentrasi sari buah nanas Ananas comosus L. Merr. dalam pengencer terhadap kualitas spermatozoa domba garut Ovis aries L. 24 jam pascakriopreservasi. Sampel semen ditampung dari lima ekor domba garut jantan satu kali dalam satu minggu menggunakan vagina buatan. Semen diencerkan dengan pengencer tris kuning telur yang mengandung sari buah nanas 0 KK, 5 KP 1, 10 KP 2, 15 KP 3, dan 20 KP 4. Semen dikemas dalam straw mini 0,25 ml dengan dosis 50 juta sel/ml. Semen diekuilibrasi pada suhu 5oC selama dua jam, kemudian dibekukan dan disimpan dalam nitrogen cair. Parameter yang dievaluasi adalah motilitas, viabilitas, integritas membran, integritas akrosom, dan abnormalitas. Hasil uji ANAVA satu faktor yang dilanjutkan dengan uji Duncan menunjukkan perbedaan nyata P < 0,05 antara KK dan KP 3 terhadap persentase motilitas dan integritas membran spermatozoa. Konsentrasi sari buah nanas 15 mampu memperkecil penurunan kualitas spermatozoa berdasarkan persentase motilitas 52,19 5,32 dan integritas membran spermatozoa 38,52 4,85.

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The research aimed to find out the effect of various concentrations of pineapple Ananas comosus L. Merr. juice in extender on garut ram Ovis aries L. 24 hours postcryopreservation. Semen samples were collected from five garut rams once a week using an artificial vagina. The samples were diluted in tris egg yolk extender with pineapple juice 0 KK, 5 KP 1, 10 KP 2, 15 KP 3, and 20 KP 4. The diluted semen samples were loaded into mini straws 0,25 ml with 50 million cell ml dosage. Samples were equilibrated at 5oC for two hours, then freezed and stored in liquid nitrogen. Parameters evaluated were motility, viability, membrane integrity, acrosome integrity, and abnormality. The one factor ANOVA and continued with Duncan test showed significant differences P 0.05 between KK and KP 3 on the percentage of spermatozoa motility and membrane integrity. Pineapple juice 15 was able to minimize the reduction of spermatozoa quality based on the percentage of spermatozoa motility 52.19 5.32 and membrane integrity 38.52 4.85."

"Telah dilakukan penelitian untuk mengetahui pengaruh berbagai konsentrasi kombinasi sari buah jeruk siam Citrus nobilis Lour. dan nanas Ananas comosus L. Merr. 1:1 dalam pengencer terhadap kualitas spermatozoa domba garut Ovis aries L. 24 jam pascakriopreservasi. Sampel semen ditampung dari lima ekor domba garut jantan satu kali dalam satu minggu menggunakan vagina buatan. Semen diencerkan dengan pengencer Tris-kuning telur yang mengandung kombinasi sari buah jeruk siam dan nanas 0 KK, 5 KP 1, 10 KP 2, 15 KP 3, dan 20 KP 4. Semen dikemas dalam straw mini 0,25 ml dengan dosis 50 juta sel/ml. Semen diekuilibrasi pada suhu 5 oC selama dua jam, kemudian dibekukan dan disimpan dalam nitrogen cair. Hasil uji ANAVA satu faktor yang dilanjutkan dengan uji Duncan menunjukkan perbedaan nyata.

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The research aimed to find out the effect of various concentrations of siam orange Citrus nobilis Lour. and pineapple Ananas comosus L. Merr. juices combination 1:1 in extender on garut ram Ovis aries L. spermatozoa quality 24 hours postcryopreservation. Semen samples were collected from five garut rams once a week using an artificial vagina. The samples were diluted in Tris egg yolk extender with siam orange and pineapple juices combination 0 KK, 5 KP 1, 10 KP 2, 15 KP 3 and 20 KP 4. Diluted semen samples were loaded into mini straws 0.25 ml with 50 million cells ml dosage. Samples were equilibrated at 5 oC for two hours, then freezed and stored in liquid nitrogen. The one factor ANOVA and continued with Duncan test showed a significant differences."

"Penelitian mengenai pengaruh penambahan antioksidan glutathione GSH pada medium maturasi oosit domba garut pascakriopreservasi dengan metode slow freezing telah dilakukan sejak Maret hingga Mei 2018. Penelitian bertujuan untuk mengetahui pengaruh penambahan GSH pada medium maturasi oosit terhadap jumlah oosit matur. Oosit dimatangkan dalam medium TCM-199 yang diberi penambahan GSH sebanyak 0 mM, 0,5 mM, 1 mM, dan 1,5 mM, kemudian dibekukan menggunakan metode slow freezing dengan suhu seeding -7oC. Oosit matur ditunjukkan oleh terbentuknya badan polar I dan atau terjadinya ekspansi sel kumulus. Data viabilitas oosit pascakriopreservasi diperoleh dengan menggunakan pewarna fluorescence Hoechst/PI, sedangkan data kualitas oosit pascakriopreservasi diperoleh berdasarkan morfologi oosit. Hasil penelitian menunjukkan penambahan antioksidan GSH pada medium maturasi oosit secara statistik berpengaruh nyata P le;0,05 pada data oosit matur. Data hasil viabilitas dan kualitas oosit pascakriopreservasi oosit hasil maturasi in vitro dengan metode slow freezing tidak berpengaruh nyata secara statistik P>0,05 antarkelompok perlakuan. Kesimpulan penelitian penambahan GSH pada medium maturasi oosit domba garut secara statistik berpengaruh positif terhadap jumlah oosit yang matur.

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The study on the effect of the addition of antioxidant glutathione GSH on Garut sheep rsquo s oocyte maturation medium after cryopreservation with slow freezing method has been done since March to May 2018. The study was conducted to determine the effect of the addition of GSH on medium maturation towards the percentage of mature oocyte. Oocytes were matured in a TCM 199 which added GSH of 0 mM, 0,5 mM, 1 mM, and 1,5 mM, then frozen using the slow freezing method and the seeding temperature used was 7oC. The mature oocyte indicated by the formation of polar body I and or the expansion of cumulus. Data of viability oocytes after cryopreservation was obtained by using Hoechst PI fluorescence dye, whereas data of quality oocytes after cryopreservation was obtained based on oocyte morphology.

The results showed that the addition of GSH antioxidant in oocyte maturation medium significantly affect P le 0,05 The result data of viability and quality of oocytes after cryopreservation of in vitro oocyte maturation by slow freezing method did not significantly affect P 0.05 statistically among treatment groups. The conclusion of the study is the addition of GSH on maturation oocyte garut sheep medium does have a positive effect statistically on the number of mature oocytes."

"ABSTRAKPenelitian dilakukan untuk mengevaluasi penambahan antioksidan alfa tokoferol dalam media pembekuan lambat terhadap kualitas oosit domba garut hingga kriopreservasi menggunakan metode pembekuan lambat. Sebanyak 226 oosit kualitas A dan B dimatangkan sebelumnya pada media pematangan TCM-199 dengan penambahan alfa tokoferol 150 μM, kemudian diawetkan dalam media pembekuan lambat dengan penambahan alfa tokoferol 0 μM (KK), 100 μM (KP1), 150. μM (KP2), dan 200 μM (KP3). Dalam media pembekuan lambat, etilen glikol 10% dan sukrosa 0,1 M juga ditambahkan. Evaluasi oosit dilakukan setelah 7 hari disimpan dalam nitrogen cair (-196 oC), meliputi morfologi oosit dan viabilitas oosit menggunakan pewarna Hoechst dan Propidium Iodide (PI). . Berdasarkan hasil yang diperoleh, persentase oosit normal pasca kriopreservasi adalah 0 μM (67,86%), 100 μM (68,42%), 150 μM (74,58%), dan 200 μM (61,11%). Persentase oosit hidup setelah kriopreservasi adalah 0 μM (76,79%), 100 μM (80,70%), 150 μM (86,44%), dan 200 μM (70,37%). Hasil penelitian menunjukkan bahwa penambahan antioksidan alfa tokoferol tidak berpengaruh terhadap kualitas oosit pasca kriopreservasi, namun terdapat kecenderungan peningkatan kualitas seiring dengan peningkatan dosis alfa tokoferol. Pada penelitian ini penambahan 150 μM alfa tokoferol dalam media pembekuan lambat merupakan dosis terbaik dalam menjaga kualitas oosit hingga pasca kriopreservasi, walaupun tidak signifikan.ABSTRACTThe study was conducted to evaluate the addition of alpha tocopherol antioxidants in slow freezing media on the oocyte quality of arrowroot sheep until cryopreservation using the slow freezing method. A total of 226 oocytes of A and B quality were pre-ripened on the TCM-199 ripening medium with the addition of 150 μM alpha tocopherol, then preserved in slow freezing media with the addition of alpha tocopherol 0 μM (KK), 100 μM (KP1), 150. μM (KP2) , and 200 μM (KP3). In slow freezing medium, 10% ethylene glycol and 0.1 M sucrose were also added. Oocyte evaluation was carried out after 7 days of storage in liquid nitrogen (-196 oC), including oocyte morphology and oocyte viability using Hoechst and Propidium Iodide (PI) stains. . Based on the results obtained, the percentage of normal oocytes after cryopreservation were 0 μM (67.86%), 100 μM (68.42%), 150 μM (74.58%), and 200 μM (61.11%). The percentage of live oocytes after cryopreservation was 0 μM (76.79%), 100 μM (80.70%), 150 μM (86.44%), and 200 μM (70.37%). The results showed that the addition of alpha tocopherol antioxidants did not affect the quality of post-cryopreservation oocytes, but there was a tendency to increase in quality along with the increase in alpha tocopherol doses. In this study, the addition of 150 μM alpha tocopherol in slow freezing medium was the best dose in maintaining oocyte quality until post cryopreservation, although it was not significant."

"Telah dilakukan penelitian tentang penambahan antioksidan glutathione ke dalam medium pembekuan lambat terhadap kualitas oosit domba garut (Ovis aries) pascakriopreservasi. Tujuan dari penelitian ini adalah untuk mengevaluasi pengaruh penambahan antioksidan glutathione dalam media pembekuan lambat dengan konsentrasi 0,5 mM; 1 mM; 1,5 mM melawan kualitas oosit domba garut. Penelitian dilakukan di Lab. Reproduksi, Pemuliaan dan Kultur Sel Hewan LIPI, Cibinong. Kriopreservasi oosit dari 136 oosit dilakukanmenggunakan krioprotektan 10% etilen glikol dan sukrosa 0,1 M. Evaluasi oosit dilakukan setelah 7 hari penyimpanan dalam nitrogen cair (-196°C), meliputi: morfologi oosit dan viabilitas oosit menggunakan pewarna Hoechst dan propidium pewarna iodida. Berdasarkan hasil penelitian didapatkan persentase oosit normal normal pascakriopreservasi yaitu 0 mM (68,40%), 0,5 mM (66,98%), 1 mM (73,05%), dan 1,5 mM (80,58%). Persentase oosit yang layak pasca-kriopreservasi adalah 0 mM (68,40%), 0,5 mM (69,76%), 1 mM (75,55%), dan 1,5 mM (83,08%). Berdasarkan uji statistik ANOVA, didapatkan hasil bahwa tidak ada perbedaan yang signifikan antar kelompok perlakuan (P > 0,05), namun grafik persentase menunjukkan pola yang cenderung meningkat dengan penambahan konsentrasi antioksidan glutathione. Kesimpulan penelitian berdasarkan hasil yang diperoleh yaitu penambahan antioksidan glutathione dalam media pembekuan lambat tidak berpengaruh terhadap kualitas oosit domba garut pasca-kriopreservasi.Research has been carried out on the addition of glutathione as an antioxidant in slow freezing medium on the quality of post-cryopreserved arrowroot sheep (Ovis aries) oocytes. The aim of this study was to evaluate the effect of adding the antioxidant glutathione in slow freezing medium with a concentration of 0.5 mM; 1 mM; 1.5 mM against arrowroot sheep oocyte quality. The research was conducted in the Lab. Animal Cell Reproduction, Breeding and Culture LIPI, Cibinong. Oocyte cryopreservation of 136 oocytes was performedusing cryoprotectant 10% ethylene glycol and 0.1 M sucrose. Evaluation of oocytes was carried out after 7 days of storage in liquid nitrogen (-196°C), including: oocyte morphology and oocyte viability using Hoechst stain and propidium iodide dye. Based on the results, the percentage of post-cryopreserved normal oocytes was 0 mM (68.40%), 0.5 mM (66.98%), 1 mM (73.05%), and 1.5 mM (80.58%). . The percentage of viable post-cryopreservation oocytes were 0 mM (68.40%), 0.5 mM (69.76%), 1 mM (75.55%), and 1.5 mM (83.08%). Based on the ANOVA statistical test, it was found that there was no significant difference between the treatment groups (P > 0.05), but the percentage graph shows a pattern that tends to increased with the addition of the antioxidant glutathione concentration. The conclusion of the study based on the results obtained was that the addition of the antioxidant glutathione in the slow freezing medium had no effect on the post-cryopreservation quality of arrowroot sheep oocytes."

"Penelitian pemberian maltosa pada visualisasi inti oosit domba garut (Ovis aries L.) setelah maturasi dan pascakriopreservasi menggunakan metode pembekuan lambat telah dilakukan. Tujuan penelitian adalah mengevaluasi kemampuan maltosa dan menentukan konsentrasi maltosa optimum yang menginduksi inti oosit swollen pascamaturasi dan pascakriopreservasi. Pada penelitian ini digunakan domba garut (Ovis aries L.) sebagai hewan model. Penelitian disusun berdasarkan Rancangan Acak Lengkap yang terdiri atas dua uji coba, empat kelompok perlakuan, dan enam kali ulangan. Pada percobaan pertama, dilakukan pematangan oosit hingga tahap Metafase II serta dilakukan pengamatan dengan penambahan berbagai konsentrasi maltosa (0%, 1%, 3%, dan 5%). Kedua, oosit M-II yang diperoleh dilanjutkan ke tahap kriopreservasi dengan metode pembekuan lambat. Selanjutnya, dilakukan pencairan (thawing) serta dilakukan pengamatan dengan penambahan berbagai konsentrasi maltosa (0%, 1%, 3%, dan 5%). Parameter yang digunakan untuk maturasi oosit adalah Metafase II yang ditandai dengan pembentukan badan polar I. Viabilitas inti oosit swollen diamati dengan menggunakan pewarna Hoechst & PI. Sedangkan, inti oosit swollen yang ditandai dengan terdapat zona bening merupakan parameter yang digunakan pascamaturasi dan pascakriopreservasi. Hasil penelitian menunjukkan terdapat perbedaan nyata antarkonsentrasi secara statistik berdasarkan uji normalitas Shapiro-Wilk, uji homogenitas Levene, dan uji sidik ragam ANOVA (P ≥ 0,05) dan konsentrasi maltosa 3% menunjukkan persentase inti oosit swollen pascamaturasi yang tertinggi (66,14%) dan persentase inti oosit swollen pascakriopreservasi yang tertinggi (70,96%). Oleh karena itu, konsentrasi maltosa 3% merupakan konsentrasi optimum yang menyebabkan inti oosit swollen pascamaturasi dan pascakriopreservasi.

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Research on maltose administration on the oocyte nuclear visualization of garut sheep (Ovis aries L.) after maturation and post-cryopreservation using the slow freezing method has been carried out. The aim of this study was to evaluate the ability of maltose and determine the optimum concentration of maltose that induces post-maturation and post-cryopreservation of swollen oocyte nuclear. In this study, garut sheep (Ovis aries L.) were used as animal models. The study was compiled based on completely randomized design consisting of two trials, four treatment groups, and six replications. In the first experiment, to ripen the oocytes in the Metaphase II stage, observations were made with the addition of various concentrations of maltose (0%, 1%, 3%, and 5%). Second, the Metaphase II oocytes obtained were continued to the cryopreservation stage with the slow freezing method. Furthermore, it was thawed and observed by adding various concentrations of maltose (0%, 1%, 3%, and 5%). The parameter used for oocyte maturation was Metaphase II which was characterized by the formation of a Polar Body I. The nuclear viability of swollen oocytes was observed using Hoechst & PI dyes. Meanwhile, swollen oocyte nuclear which is marked by the presence of a clear zone is a parameter used post-maturation and post-cryopreservation. The results showed that there were differences between concentrations statistically based on the Shapiro-Wilk normality test, the Levene homogeneity test, and ANOVA variance test (P ≥ 0.05) and the 3% maltosa concentration showed the highest percentage of post-maturation swollen oocyte nuclear (66.14%) and the highest percentage of post-cryopreservation swollen oocyte nuclear (70.96%). Therefore, the 3% maltose concentration is the optimum concentration to cause swollen post-maturation and post-cryopreservation of oocyte nuclear."

"Penelitian ini menganalisis pengaruh pemberian sukrosa pada visualisasi inti oosit domba garut (Ovis aries) pascamaturasi dan pascakriopreservasi. Koleksi ovarium domba garut dilakukan di rumah potong hewan Sentul, Jawa Barat. Slicing ovarium dilakukan untuk dikoleksi oositnya. Grading dilakukan pada oosit yang terkoleksi. Oosit dengan grade A dan B dimaturasi menggunakan media maturasi TCM-199 dan diinkubasi selama minimal 24 jam di dalam inkubator. Total jumlah oosit yang telah mencapai metafase II (M II) setelah maturasi dibagi menjadi dua untuk dilanjutkan ke tahap kriopreservasi dalam waktu minimal satu minggu dan untuk diberi perlakuan inkubasi pada media sukrosa dengan konsentrasi 0%, 1%, 3%, dan 5% dalam waktu ±10 menit persetiap perlakuan. Setelah diberi perlakuan, oosit diamati pembengkakan pada bagian intinya dengan menggunakan mikroskop fluoresens. Hasil uji statistik Kruskal-wallis menunjukkan (P ≤ 0,05). Konsentrasi sukrosa 3% menjadi konsentrasi yang optimum untuk menginduksi pembengkakan inti oosit domba garut pascamaturasi dan pascakriopreservasi.

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This study analyzed the effect of sucrose addition on sheep oocyte (Ovis aries) for visualization of oocyte nucleus after maturation and post-cryopreservation. Sheep ovaries was collected from rumah potong hewan Sentul, Jawa Barat. Ovaries were sliced and oocyte was collected from ovaries. The collected oocytes were grading. Grade A and B oocytes were matured using maturation media TCM-199 and incubated for at least 24 hours in the incubator. The total number of oocytes that have reached metaphase II (M II) after maturation is divided into two for development to the cryopreservation stage in at least one week and to be given an incubation treatment on sucrose media with concentrations of 0%, 1%, 3%, and 5% in time ± 10 minutes per each treatment. After being treated, the oocyte was observed for swelling at its core using a fluorescence microscope. Kruskal-wallis test showed that (P ≤ 0,05). The concentration of sucrose 3% becomes the optimal concentration to induce swelling of the oocyte core of garut sheep after maturation and post-cryopreservation. "

"Penelitian ini menganalisis pengaruh pemberian sukrosa pada visualisasi inti oosit domba garut (Ovis aries) pascamaturasi dan pascakriopreservasi. Koleksi ovarium domba garut dilakukan di rumah potong hewan Sentul, Jawa Barat. Slicing ovarium dilakukan untuk dikoleksi oositnya. Grading dilakukan pada oosit yang terkoleksi. Oosit dengan grade A dan B dimaturasi menggunakan media maturasi TCM-199 dan diinkubasi selama minimal 24 jam di dalam inkubator. Total jumlah oosit yang telah mencapai metafase II (M II) setelah maturasi dibagi menjadi dua untuk dilanjutkan ke tahap kriopreservasi dalam waktu minimal satu minggu dan untuk diberi perlakuan inkubasi pada media sukrosa dengan konsentrasi 0%, 1%, 3%, dan 5% dalam waktu ±10 menit persetiap perlakuan. Setelah diberi perlakuan, oosit diamati pembengkakan pada bagian intinya dengan menggunakan mikroskop fluoresens. Hasil uji statistik Kruskal-wallis menunjukkan (P ≤ 0,05). Konsentrasi sukrosa 3% menjadi konsentrasi yang optimum untuk menginduksi pembengkakan inti oosit domba garut pascamaturasi dan pascakriopreservasi.

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This study analyzed the effect of sucrose addition on sheep oocyte (Ovis aries) for visualization of oocyte nucleus after maturation and post-cryopreservation. Sheep ovaries was collected from rumah potong hewan Sentul, Jawa Barat. Ovaries were sliced and oocyte was collected from ovaries. The collected oocytes were grading. Grade A and B oocytes were matured using maturation media TCM-199 and incubated for at least 24 hours in the incubator. The total number of oocytes that have reached metaphase II (M II) after maturation is divided into two for development to the cryopreservation stage in at least one week and to be given an incubation treatment on sucrose media with concentrations of 0%, 1%, 3%, and 5% in time ± 10 minutes per each treatment. After being treated, the oocyte was observed for swelling at its core using a fluorescence microscope. Kruskal-wallis test showed that (P ≤ 0,05). The concentration of sucrose 3% becomes the optimal concentration to induce swelling of the oocyte core of garut sheep after maturation and post-cryopreservation. "