Ditemukan 2 dokumen yang sesuai dengan query
Ni Gusti Agung Ayu Putu Tika Andayani
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ABSTRAKPendahuluan: Kanker kolorektal masih menjadi penyebab kematian yang tinggi di dunia. Tatalaksana kanker kolorektal masih memiliki banyak efek samping. Perikarp buah Garcinia mangostana banyak mengandung xanthone yang memiliki efek antioksidan dan antikanker. Pada penelitian eksperimental ini, mikropartikel ekstrak Garcinia mangostana dibungkus kapsul kitosan alginat yang bersifat mukoadesif terhadap kolon dan protektif terhadap asam lambung. Metode: mencit BALB/c dibagi menjadi tiga kelompok, yaitu: kontrol, perlakuan yang terdiri dari tiga dosis ekstrak Garcinia mangostana (2 g/kgBB, 1 g/kgBB, dan 0,5 g/kgBB), dan satelit (kontrol dan 2 g/kgBB). Fungsi ginjal mencit dilihat dari kadar BUN dan kreatininnya setelah diberikan perlakuan selama 14 hari untuk kelompok kontrol dan perlakuan serta selama 28 hari untuk kelompok satelit untuk melihat efek reversibelnya. Sebagai data tambahan, berat ginjal setelah perlakuan dan gejala toksisitas klinis yang muncul selama perlakuan juga diamati pada penelitian ini. Hasil: Kadar BUN dan kreatinin ditemukan signifikan pada mencit jantan (p = 0,021 dan p = 0,083) pada dosis 2 g/kgBB jika dibandingkan dengan kelompok kontrol. Efek reversibel terlihat pada nilai kadar kreatinin mencit jantan. Garcinia mangostana tidak berpengaruh pada berat ginjal. Tanda toksisitas klinis tidak tampak selama proses perlakuan. Kesimpulan: Pemberian sediaan mikroenkapsulasi dari fraksi etil-asetat ekstrak Garcinia mangostana Linn meningkatkan kadar BUN dan kreatinin jika diberikan pada dosis 2 g/kgBB selama 14 hari pada mencit jantan.
ABSTRACTntroduction: Colorectal cancer is one of the highest caused of death in the world. Colorectal cancer managements still have many side effects. The pericarp of the Garcinia mangostana contains xanthones that constitute antioxidant and anticancer effects. In this experimental study, the microparticles of Garcinia mangostana extract were wrapped in chitosan alginate capsules which are mucoadhesive against the colon and protective against gastric acid. Methods: BALB/c mice were divided into three groups, they were: control, treatment consisting of three doses (2 g/kg BW, 1 g/kg BW, and 0.5 g/kg BW) of Garcinia mangostana extract, and satellite (control and 2 g/kg BW). Renal function of mice was seen from BUN and creatinine levels after being treated for 14 days for the control group and treatment group, and for 28 days for the satellite group, to see their reversible effect. As additional data, kidney weight after treatment and symptoms of clinical toxicity that emerged during treatment were also observed in this study. Results: BUN and creatinine levels were found to be significant in male mice (p = 0.021 and p = 0.083) at a doses of 2 g/ kg BW when compared to the control group. Reversible effect seen on creatinine levels in male mice. Garcinia mangostana does not affect kidney weight. Signs of clinical toxicity were not seen during the treatment process. Conclusion: Microencapsulation of the ethyl-acetate fraction of Garcinia mangostana Linn increased levels of BUN and creatinine if given at 2g/ BW dose for 14 days in male mice."
Depok: Fakultas Kedokteran Universitas Indonesia, 2019
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Ardhea Pramesti Ningrum
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ABSTRAKAcute Kidney Injury (AKI) merupakan sindrom penurunan fungsi ginjal dalam mengatur keseimbangan cairan dan elektrolit tubuh, serta eksresi zat sisa metabolisme secara tiba-tiba, yang ditandai dengan penurunan fungsi ginjal dalam beberapa hari. Dalam patofisiologinya,terdapat 3 jenis AKI yaitu AKI pra-renal, intrinsik, dan post-renal. Salah satu penyebab AKI adalah kondisi Ischemia Reperfusion Injury (IRI). IRI merupakan kondisi kerusakan jaringan yang disebabkan aliran darah balik ke jaringan setelah terjadi iskemia (anoksia, hipoksia). Iskemia yang terjadi pada jaringan ginjal menyebabkan berbagai kondisi yang berakibat pada stres oksidatif dan inflamasi. Penelitian ini bertujuan untuk mengetahui profil BUN dan kreatinin pada tikus model Renal Ischemia-Reperfusion Injury. Sebanyak 24 ekor tikus jantan galur Sparague Dawley yang dibagi menjadi 4 kelompok yaitu kelompok normal (sham), iskemia 15 menit, 30, dan 45 menit. Setiap kelompok terdiri atas 6 tikus dengan berat badan antara 150-200gram. Induksi Renal Ischemia-Reperfusion Injury dilakukan dengan metode bilateral renal pedicle clamping. Pengamatan dilakukan sebelum dilaksanakan perlakuan atau jam ke 0 serta di jam ke 24, 48, dan minggu kedua setelah induksi melalui kadar kreatinin serum dan kadar BUN serum. Data diolah secara statistik secara SPSS dengan one way ANOVA method. Induksi Ischemia Reperfusion Injury selama 15 menit menyebabkan peningkatan kadar serum kreatinin dan BUN pada jam ke 24 (p<0,05), 48 (p<0,05) serta penurunan pada minggu ke 2 (p>0,05). Sedangkan pada Induksi Ischemia Reperfusion Injury selama 30 menit, peningkatan kadar serum kreatinin kadar BUN baru terjadi di jam ke 48 (p<0,05) serta penurunan di minggu ke dua (p>0,05). Berdasarkan hasil tersebut, induksi Ischemia Reperfusion Injury menyebabkan peningkatan kadar kreatinin dan BUN pada 24 jam setelah reperfusi serta penurunan pada 14 hari setelah reperfusi
ABSTRACTAcute Kidney Injury (AKI) is a syndrome of decreased kidney function in regulating the body's fluid and electrolyte balance, as well as sudden excretion of metabolic waste, which is characterized by a decrease in kidney function within a few days. In its pathophysiology, there are 3 types of AKI namely pre-renal, intrinsic, and post-renal AKI. One of the causes of AKI is the condition of Ischemia Reperfusion Injury (IRI). IRI is a condition of tissue damage caused by blood flow back to the tissue after ischemia (anoxia, hypoxia). Ischemia that occurs in kidney tissue causes various conditions that result in oxidative stress and inflammation. This study aims to determine the profile of BUN and creatinine level as a biochemical marker for kidney disease in Renal Ischemia-Reperfusion Injury rat model. A total of 24 Sparague Dawley male rats were divided into 4 groups: normal (sham), ischemic 15 minutes, 30, and 45 minutes. Each group consists of 6 rats weighing between 150-200 gram. Induction of Renal Ischemia-Reperfusion Injury is performed using bilateral renal pedicle clamping method. Observations were made before the treatment or the 0th hour and at 24, 48, and the second week after induction through creatinine serum levels and BUN serum levels. The data is processed statistically by SPSS with the one way ANOVA method. Induction of Ischemia Injury Reperfusion for 15 minutes caused an increase in serum creatinine and BUN levels at 24 hours (p <0.05), 48 (p <0.05) and replacement at week 2 (p> 0.05). Whereas in the induction of Ischemic Reperfusion Injury for 30 minutes, the increase in serum BUN creatinine levels occurred at 48 hours (p <0.05) and decreased in the second week (p> 0.05). Based on these results, the induction of Reperfusion Ischemia Injury caused an increase in creatinine and BUN levels 24 hours after reperfusion and decreased 14 days after reperfusion."
2020
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